Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

... lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin Gustav Vaaje-Kolstad, Anne C. Bunæs, Geir Mathiesen and Vincent ... instructions, containing ends compatible with the expression vector (forward primer, 5¢-GGTATTGAGGGTCGCCATGGTTATGTTC AATCACCA-3¢; reverse primer, 5¢-AGAG...

Ngày tải lên: 18/02/2014, 08:20

... immunoprecipitations and can activate tran- scription of the promoter in transient transfection assays through the upstream part of the SIRF element. Mutational analysis of a putative CDE ⁄ CHR site in the ... usually lack a TATA-box and employ multiple transcriptional start sites [10,14]. Mutation of either a CDE or a CHR in a promoter leads to the activation...

Ngày tải lên: 16/02/2014, 09:20

... phosphorylation. The data presented here suggest that the pro-domain of BMP-2 can alter the signalling properties of the growth factor by modulating the ability of the mature part to interact with the ... Ghayor C, Suzuki A, Palmer G & Caverzasio J (2003) Activation of p38 mitogen- activated protein kinase and c-Jun-NH 2 -terminal kinase by BMP-2 and their i...

Ngày tải lên: 18/02/2014, 06:20

... picolinic acid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzyme stands at a branch point of the tryptophan to NAD pathway, and deter- mines the final fate of the amino acid, i.e. transformation ... insertion domain that comprises a short a- helix and a three-stranded anti-parallel b-sheet; the remaining protein residues form the (a ⁄ b) 8...

Ngày tải lên: 18/02/2014, 06:20

... 5¢-TAA TAT TAG CAT ATG GTG ACC GCT TTC TAT TGG GCG CTG CGT AAA CGT CGT AAA CCG GGT AGC- 3¢ and 5¢-TAG TAG GGA TCC TCA TTA AAC GAT GTA TTC CAT ACG GTT CAG GCT-3¢. The forward primer contains a NdeI ... ligands are membrane-spanning proteins made of a large extracellu- lar region, a transmembrane segment, and a  100–200 residue cytoplasmic tail. The intracellular region of...

Ngày tải lên: 18/02/2014, 16:20

... literature [8,37]. The errors are calculated from the propagation of fitting errors. DC p ¢ was obtained from fitting of the thermal- and urea-denaturation data (using the approach of Pace and Laurents ... calorimet- rically. In both cases, data analyses involves the extra- polation of the thermodynamic parameters to standard conditions, usually 298 K in the absence o...

Ngày tải lên: 19/02/2014, 12:20

... extrusion and uptake of Na + and K + ions across plasma membranes of mammalian cells. The enzyme is a heterodimer of a 100 kDa catalytic subunit and a heavily glycosylated b subunit of about 55 kDa [1–3]. Ouabain, ... were usually complementary. The primer sequence of the N398D construct was GCTGACACCA CAGAG GATCAGAGTGGGGTCTCC and that of the D36 9A constr...

Ngày tải lên: 19/02/2014, 16:20

... structure and immediately suggested several important new RNA folds and rationales of RNA tertiary and quater- nary structure that had not hitherto been appreciated [5,7,8]. A wealth of new information ... whether the functional effects of these mutations were due to the architectural and sta- bilizing role of the proteins alone, i.e. that perturba- tion of prot...

Ngày tải lên: 19/02/2014, 17:20

... values of the lattice as a measure of the a nity of attachment phrase and attachment node. The intuition is that we are looking for the strongest evidence available for the attach- ment. The strongest ... high attachment (highA), low at- tachment (lowA), verb attachment (verbA), and noun attachment (nounA) according to the gold standard. All instances of RC and...

Ngày tải lên: 20/02/2014, 12:20

... compiled into an efficient C program encoding a deterministic finite state transducer. The analyser takes a word form and CLAWS tag and returns a lemma plus any inflec- tional affixes. The type and token ... bootstrapping methods which utilise an unlabelled bracketing of the Susanne Treebank (Watson et al., 2006). This makes the system more easily retrainable after changes...

Ngày tải lên: 20/02/2014, 12:20