Tài liệu Báo cáo khoa học: a-Defensins increase lung ﬁbroblast proliferation and collagen synthesis via the b-catenin signaling pathway doc

... among lung ﬁbroblasts.Inhibition of the b-catenin signaling pathway using quercetin blocked the a-defensin-induced increase in lung ﬁbroblast proliferation and collagen synthesis To investigate the ... compilation ª 2009 FEBS a-Defensins increase lung ﬁbroblast proliferation and collagen synthesis via the b-catenin signaling pathway Weihong Han1, Wei Wang2, Kamal A. Mohammed2,3 and Yunchao Su1,4,5,61 ... proliferation and collagen synthesis involve the b-catenin signaling pathway. Inhibition of b-catenin signaling pathway usingquercetin blocked the a-defensin-induced increase in collagen release from lung...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... chromatography and subsequent throm-bin cleavage [41]. The use of thrombin signiﬁcantlyincreases the cost of the puriﬁcation, and the require-ment for HPLC increases the length and complexity of the ... 5¢-CCTGCCGAGCTCCTATTACACAACGCCACCAACCATCAG-3¢. The PCR solution was prepared in the buffer suppliedwith the enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 ... as with the studies by Lee et al. [21] and Subramanian and Shree[42], there was no rigorous assessment of the purity of the product or the correctness of the sequence. In con-trast, the puriﬁcation...

Tài liệu Báo cáo khoa học: Is ATP binding responsible for initiating drug translocation by the multidrug transporter ABCG2? docx

... malignancies. One of the most prevalent primary cellular defence mechanismsagainst chemotherapeutic agents is the membrane-bound transporter [1]. The deﬁning feature of thesetransporters is their ability ... speciﬁcity compared with other multidrug trans-porters. In particular, the protein confers resistance to the anticancer drugs mitoxantrone [17], methotrexate[18] and the camptothecins [19]. Although ... best evidence for an interaction between the two domains is the ability of numerous substrates and modulators of ABCG2 (and the R482G isoform)to stimulate the rate of ATP hydrolysis [21,24,25],albeit...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... consumption and to examine the possible role played by cells and mole-cules of the immunological system in hepatic injury and repair. Understanding the interdependence between the metabolism of iron and ... localizes in the cytoplasm ofhuman epithelial cells of the gastrointestinal tract and liver, two central organs in the regulation of ironmetabolism [20,21]. After their synthesis in the endo-plasmic ... receptor and ferritin; (b) developed iron accumulation and (c) increased the prooxidant state and the level ofoxidatively modiﬁed proteins. These results are inaccordance with previous in vitro and...

Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt

... nervous system injury, and reducing the biosynthesis ofICAM-1 and other cytokines by blocking the cell signal pathway mightprovide a new strategy against inﬂammatory and immune reaction afterperipheral ... expression via activation of the JNK pathway and activator protein-1 [45], the pres-ent research suggests that the JNK pathway also plays asigniﬁcant role in the signaling cascade leading to ... occurred via activation of MAPKs, especially the p38 and SAPK ⁄ JNK pathways. Activation of MAPK pathwaysmight be a precondition for induction of ICAM-1expression. Reducing the biosynthesis of...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... individualswith mild disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of defective BTK signaling in humanshas been described ... COS7 and 293Tcells and did not vary like BTK–SYK; therefore, the differences in the PH–TH domains remain the decisivefactor for this variation. The B-cell adapter protein BLNK (SLP-65) and itsT-cell ... phosphatidylino-sitol-3,4,5-trisphosphate, thereby bringing them inclose proximity to other membrane-tethered signaling proteins. In SYK, the linker B region contains keytyrosines that are subject to auto- and ⁄ or transphosph-orylation,...

Tài liệu Báo cáo khoa học: a-enolase: a promising therapeutic and diagnostic tumor target ppt

... metalloenzyme that catalyzes the dehydra-tion of 2-phospho-d-glycerate to phosphoenolpyruvatein the second half of the glycolytic pathway. In the reverse reaction (anabolic pathway) , which occurs ... involved in the synthesis of pyru-vate. It also acts as a plasminogen receptor and thus mediates activation ofplasmin and extracellular matrix degradation. In tumor cells, EMOA isupregulated and supports ... genes, ENO1, ENO2 and ENO3 encode forthree isoforms of the enzyme, a-enolase (ENOA),c-enolase and b-enolase, respectively, with highsequence identity [4–6]. The expression of these iso-forms...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR-targeted anticancer therapy doc

... PDGFRA and T670I inKIT) [27–29]. The conserved threonine residues inthese different kinases are located near the kinaseactive site and appear to be critical for the binding ofATP and the corresponding ... genotype-directedtherapy in this disease.KRAS mutation as a mechanism ofresistance to EGFR-targeted therapy The KRAS protein is localized to the inner surface of the cell membrane. The binding of ligand ... residues surrounding the ATP-binding cleft of the tyrosine kinasedomain of the receptor and thereby stabilize the interaction with EGF-TKIs.Table 2. Prospective study of EGFR-TKI monotherapy for NSCLCpatients...

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... protection, deﬁne the core of the protein’s Nstate [21]. For the T domain, these were the center ofTH5 and TH8, and the N-terminal half of TH9. Alla-helices (TH1, 3, 4, 5’, 6, 7 and the remaining ... highlighting the regions of the protein with either higher stability and ⁄ or lower accessi-bility to the solvent. The regions exhibiting the lowestexchange (below 40% D occupancy), in other words, the ... In the case of the T domain, the MGstate corresponds to the functional state, which initi-ates the translocation of the catalytic domain. Here, the data allowed identiﬁcation of the core of the...

Tài liệu Báo cáo khoa học: Paradoxical interactions between modiﬁers and elastase-2 Patricia Schenker and Antonio Baici docx

... with the modiﬁers separately and then in various combinations of concentrations. In the case of the sulfated polysaccharides, the effectormolecules are constituents of the same sample, and their ... Equation (1) was ﬁtted to the data, and the solid lines represent the best ﬁt. Parameters from regressionanalysis are shown together with theirstandard errors in (A–D). The substrate wasMeOSuc-AAPV-pNA ... blue, and the active site is shown in red. The positive charges form three beltsaround the enzyme molecule, which is shown from the front(A) and the back (B). Arg217A is positioned along the...

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