Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
... indicate bands of recombinant calpain 7MIT and GST, GST IST1, GST–IST1MIM, and GST–IST1MIM L326D,L353A , respectively. Enhancement of calpain 7 autolysis by IST1 Y. Osako et al. 4416 FEBS Journal 277 (2010) ... GST-fused proteins (GST– IST1, GST–IST1MIM, GST–IST1MIM L326D,L353A , GST– CHMP6NT, GST–pHis and GST–MIT–pHis). Expression of GST IST1, GST–IST1MIM and GST IST1 L326D...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... A328F, and 28 70 0 ± 70 0 min )1 for A328W. This activity is titratable, with pK a values of 6.0–6.6, suggesting that the catalytic histidine is protonated at pH 5. The existence of activity when ... bk cat are consistent with titration of the catalytic histidine, H438(440). The persistence of activity in the presence of the protonated form of the catalytic histidine...
Ngày tải lên: 20/02/2014, 23:20
Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt
... of Biochemistry, Lund University, Sweden During biosynthesis of chlorophyll, Mg 2+ is inserted into protoporphyrin IX by magnesium chelatase. This enzyme consists of three different subunits of ... AAA + proteins represent one type of molecular chaperones and their function is to control the fate of proteins or DNA. This is done by facilitating protein folding and unfol...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... Structures of thymidine kinase 1 of human and mycoplasmic origin. Proc Natl Acad Sci USA 101, 179 70– 179 75. 15 Sabini E, Ort S, Monnerjahn C, Konrad M & Lavie A (2003) Structure of human dCK ... the synthesis of dTTP by transfer of a c-phosphate group from a nucleoside triphosphate to the 5¢-hydroxyl group of thymidine, forming dTMP. Human TK1 is cytosolic and its...
Ngày tải lên: 18/02/2014, 12:20
Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... Yoshida T & Kikuchi G (1 979 ) Mechan- ism of increase of heme oxygenase activity induced by hemin in cultured pig alveolar macrophages. Arch Bio- chem Biophys 1 97, 6 07 6 17. 9 Okinaga S, Takahashi ... Shibahara S (1988) Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur J Biochem 171 , 4 57 461. 26 McCoubrey WK Jr, Huang TJ & Maines MD (19 97) Heme ox...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... Germany In humans, isolated deficiencies of each of the six dif- ferent steps within the leucine degradation pathway (Fig. 1) cause their own characteristic disease [1]. The enzymes of this pathway ... aerobically grows on isovalerate as sole car- bon and energy source. Isovalerate is activated by a CoA-transferase (2.8.3.–) to give isovaleryl-CoA (Fig. 1). Isovalerate is metaboliz...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc
... carbonyl group of Asn7 of Tat(1–9) and the side chain of Lys554 of DP IV and finally, between the carbonyl group of Ile8 of the peptide interacting with the Tyr752 hydroxyl group of the enzyme. ... hydrolysis of IL-2(1–12) and the inhibition of mitogen-induced prolifer- ation of human T cells by Tat(1–9) [22]. Amino acid substitutions at positions 5 and 6 of Tat(1–9)...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... dopamine receptor/G protein interaction. Evidence for agonist selection of G protein subtype. J. Biol. Chem. 276 , 286 67 28 675 . 31. Gazi, L., Nickolls, S.A. & Strange, P.G. (2003) Functional cou- pling of ... SEM, fmolÆmg )1 of protein) pK d (mean ± SEM, K d ,p M ) Sf9-HIV-D 2L 1445 ± 2 17 10.14 ± 0.06 (72 ) Sf9-FLAG-D 2L 72 8 ± 88 10.01 ± 0.08 (100) Sf9-HIV-D 2S 178 5...
Ngày tải lên: 21/02/2014, 00:20
Tài liệu Báo cáo khoa học: "The Structure of User-Adviser Dialogues: Is there Method in their Madness?" pdf
... dialogue structure on the basis of an analysis of the task and an analysis of the users' and adviser's plans and goals. BOUNDARY MARKERS The analysis of boundary markers revealed ... distinguishes three structures in a discourse structure: 1) the structure of the sequence of utterances, 2) the structure of the intentions conveyed, and 3) the attentional state. D...
Ngày tải lên: 21/02/2014, 20:20
Báo cáo khoa học: The activity of Plasmodium falciparum arginase is mediated by a novel inter-monomer salt-bridge between Glu295–Arg404 doc
... manganese cluster of arginase I. Biochemistry 42, 77 48 77 58. 48 Carvajal N, Venegas A, Oestreicher G & Plaza M (1 971 ) Effect of manganese on the quaternary structure of human liver arginase. ... Curr Opin Infect Dis 21, 468– 475 . 67 Sali A (1993) Comparative protein modelling by satis- faction of spatial restraints. J Mol Biol 234, 77 9–815. 68 Marti-Renom MA, Stuar...
Ngày tải lên: 29/03/2014, 23:20