Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... 93,395–402.Structure of C-peptide oligomeric states J. Lind et al.3768 FEBS Journal 277 (2010) 3759–3768 ª 2010 The Authors Journal compilation ª 2010 FEBS Structural features of proinsulin C-peptide oligomeric ... lM C-peptide in the presence of insulin (C) and of 100 lM C-peptide in the presence of NaCl and formamide (D) under native conditions.J. Lind et al. Structure of C-peptide oligomeric states FEBS Journal ... time and analyzedunder native conditions. Oligomer distribu-tion of 100 lM proinsulin C-peptide in thepresence of divalent Ca2+ and Mg2+ionsunder native conditions (B), and of 100 lMC-peptide...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... selection of recombinants. The sitesincorporated were HaeIII, NcoI and BamHI (Table 1). ThePCR mix contained 200 ng of each primer, 20 ng of the tem-plate, 200 lm of each dNTP and 5 units of Taq ... Department of Biotechnology(DBT), Department of science and technology (DST),Council of Scientiﬁc and Industrial research (CSIR) and senior research fellowship from CSIR, Govern-ment of India.References1 ... Two features of the Y74W* mutant of PfTIM need to be rationalized: (a) the reduced stability of the dimeric structure, and (b) the signiﬁcantly lowervalue of kcat, suggesting an impairment of...

Tài liệu Báo cáo khoa học: Unique features of recombinant heme oxygenase of Drosophila melanogaster compared with those of other heme oxygenases studied docx

... His25works as the proximal ligand of heme iron. The His39residue of DmHO corresponds to His25 of rat HO-1 and therefore is likely to be the proximal ligand. The crystalstructure of human HO-1 [39] shows ... and two peaks at538 and 575 nm in the visible region, indicating formation of an oxy form. Formation of the oxy form is faster than itsfurther degradation reaction, and loss of the Soret band ... with NdeIandHindIIIandthenclonedintotheNdeIandHindIII sites of thepMW172 expression vector. The constructs encoding the fulllength and C-terminally truncated DmHO were namedpMWDmHO and pMWDmDHO,...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... consisting of four copies of foursubunits, a, b, c and d. The a and b subunits are regu-latory and are the sites of phosphorylation and meta-bolite binding and are also regulated by the binding of ... R2–1.079|cN ⁄ cH |R1(1–NOE), and cN and cH are the gyro-magnetic ratios for1H and 15N. The calculation of R2¢ and R1¢ subtracts contributions from the high frequency com-ponents of local motions. The ... (1992) High and intermediate afﬁnity calmodulin binding domains of the alpha and beta subunits of phosphorylase kinase and their potential role in phosphorylation-dependentactivation of the holoenzyme....

Tài liệu Báo cáo khoa học: "Structural Definition of Affixes from Multisyllable Words" docx

... L. Resnikoff and J. L. Dolby, "The Nature of Affixing in Written English," an algorithm for the structural definition of affixes was developed and applied to data consisting of all ... criterion of four or more words in establishing an affix probability and of two or more consonant strings in defining an affix from a prob- ability was established by Dolby and Resnikoff. This ... perhaps, is the structural classification of the weak suffixes by degree of weakness as a means of approaching the suffix-in- context problem. Table 2, which reviews the prefixes and suffixes...

Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... initiation and development of both ﬁbrinolysis and cell detachment-induced apopto-sis and may therefore be of relevance during athero-thrombosis and inﬂammatory states. ResultsContribution of plasminogen ... 6, Glu-Pg- and mAb 34D3-trea-ted cells. Bands in lanes 1 and 2 correspond to forms I and II of plasmin(ogen). High molecular weight bands in lanes 5 and 6 correspond tomAb A10.2- and mAb 34D3-plasminogen ... the presence of varying concentrations of mAb A10.2 or mAb 34D3 (0–1 lM for cells and 0–0.25 lM for ﬁbrin) and ﬁxedconcentrations of Glu-Pg (200 nM for cells and 50 nM for ﬁbrin) and CBS0065...

Tài liệu Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents pptx

... developed and showedboth b chains and equivalent levels of laminin c1chainsinEDTA/detergent extracts of various mouse tissues. Thecontent of b1 chains in these extracts exceeded the conten t of b2 ... each other and allowedestablishment of b chain-speci®c radioimmunoassays and light and electron microscopic immunostaining of t issues.This demonstrated a 5±25-fold lower content of b2com-pared ... synapses and glomer-ular ®ltration [23,24].In this study, we have used recombinant production of domain IV in mammalian cells to explore further structural and tissue differences of the b1andb2...

Tài liệu Báo cáo khoa học: "Generative Power of CCGs with Generalized Type-Raised Categories" pptx

... gories (GTRC) and each ai of a GTRC an argument (of the GTRC). The introduction of GTRCs affects the use of combi- natory rules: functional application ">: z/y + y , z" and generalized ... below where g, ta and ~a,rc re- present the classes of the instances of CCG-Std and CCG- GTRC, respectively: Definition 1 Gatrc is the collection of G's (extension of a G E G, ta) such ... Linear Index Grammars and Tree Adjoining Grammars (Joshi, Vijay- Shanker, and Weir, 1991; Vijay-Shanker and Weir, 1994). But CCG-Std cannot handle the generalization of type raising that has...

Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf

... thecleavages of the sites were in the order: Tyr146/Asn147 !Phe114 ! other sites. The weakness of bands of fragments I and IV and the appearance of fragment II in the degradation of the Tyr146!His/Asn147!Ser ... amount of trypsin.DISCUSSIONThe structural determinants of autolysis and autolyticinactivation of rat D-chymotrypsin (a propeptide deﬁcientvariant of the wild-type enzyme, see Materials and ... studies of a number of proteolytic sites in various proteins, Hubbard and coworkersFig. 4. Cleavage order of autolytic sites of rat D-chymotrypsin. Thetwo domains of the molecule are shaded and...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... Rouvinen1 and Nina Hakulinen11 Department of Chemistry, University of Eastern Finland, Joensuu, Finland2 VTT Technical Research Centre of Finland, Espoo, FinlandKeywordsascomycete; C-terminal plug; ... buf-fer at pH 4.5 and in 40 mM Mes buffer at pH 6.0 (25 °C). Structural formulas of the substrates are presented. Redox potentials (E°)ofT1coppers of the laccases and redox potentials of the substrates ... round of reﬁnement were R = 26.7% and Rfree= 34.9%. When atwin operator was included, R-value and Rfree-value weredecreased to 19.2% and 25.2%, respectively. Reﬁnement of the model and twin...

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