Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... 93, 395–402. Structure of C-peptide oligomeric states J. Lind et al. 3768 FEBS Journal 277 (2010) 3759–3768 ª 2010 The Authors Journal compilation ª 2010 FEBS Structural features of proinsulin C-peptide oligomeric ... l M C-peptide in the presence of insulin (C) and of 100 l M C-peptide in the presence of NaCl and formamide (D) under native conditions. J. Lin...

Ngày tải lên: 18/02/2014, 04:20

... selection of recombinants. The sites incorporated were HaeIII, NcoI and BamHI (Table 1). The PCR mix contained 200 ng of each primer, 20 ng of the tem- plate, 200 lm of each dNTP and 5 units of Taq ... Department of Biotechnology (DBT), Department of science and technology (DST), Council of Scientific and Industrial research (CSIR) and senior research fellowship from...

Ngày tải lên: 18/02/2014, 11:20

... His25 works as the proximal ligand of heme iron. The His39 residue of DmHO corresponds to His25 of rat HO-1 and therefore is likely to be the proximal ligand. The crystal structure of human HO-1 [39] shows ... and two peaks at 538 and 575 nm in the visible region, indicating formation of an oxy form. Formation of the oxy form is faster than its further degradation reaction,...

Ngày tải lên: 19/02/2014, 12:20

... consisting of four copies of four subunits, a, b, c and d. The a and b subunits are regu- latory and are the sites of phosphorylation and meta- bolite binding and are also regulated by the binding of ... R2– 1.079|cN ⁄ cH |R1(1–NOE), and cN and cH are the gyro- magnetic ratios for 1 H and 15 N. The calculation of R2¢ and R1¢ subtracts contributions from the high fr...

Ngày tải lên: 19/02/2014, 17:20

... L. Resnikoff and J. L. Dolby, "The Nature of Affixing in Written English," an algorithm for the structural definition of affixes was developed and applied to data consisting of all ... criterion of four or more words in establishing an affix probability and of two or more consonant strings in defining an affix from a prob- ability was established by Dolby and...

Ngày tải lên: 19/02/2014, 19:20

... initiation and development of both fibrinolysis and cell detachment-induced apopto- sis and may therefore be of relevance during athero- thrombosis and inflammatory states. Results Contribution of plasminogen ... 6, Glu-Pg- and mAb 34D3-trea- ted cells. Bands in lanes 1 and 2 correspond to forms I and II of plasmin(ogen). High molecular weight bands in lanes 5 and 6 co...

Ngày tải lên: 20/02/2014, 01:20

... developed and showed both b chains and equivalent levels of laminin c1chainsin EDTA/detergent extracts of various mouse tissues. The content of b1 chains in these extracts exceeded the conten t of b2 ... each other and allowed establishment of b chain-speci®c radioimmunoassays and light and electron microscopic immunostaining of t issues. This demonstrated a 5±25-fold lo...

Ngày tải lên: 21/02/2014, 03:20

... gories (GTRC) and each ai of a GTRC an argument (of the GTRC). The introduction of GTRCs affects the use of combi- natory rules: functional application ">: z/y + y , z" and generalized ... below where g, ta and ~a,rc re- present the classes of the instances of CCG-Std and CCG- GTRC, respectively: Definition 1 Gatrc is the collection of G's (ext...

Ngày tải lên: 22/02/2014, 03:20

... the cleavages of the sites were in the order: Tyr146/Asn147 ! Phe114 ! other sites. The weakness of bands of fragments I and IV and the appearance of fragment II in the degradation of the Tyr146!His/Asn147!Ser ... amount of trypsin. DISCUSSION The structural determinants of autolysis and autolytic inactivation of rat D-chymotrypsin (a propeptide deficient variant of t...

Ngày tải lên: 22/02/2014, 07:20

... Rouvinen 1 and Nina Hakulinen 1 1 Department of Chemistry, University of Eastern Finland, Joensuu, Finland 2 VTT Technical Research Centre of Finland, Espoo, Finland Keywords ascomycete; C-terminal plug; ... buf- fer at pH 4.5 and in 40 m M Mes buffer at pH 6.0 (25 °C). Structural formulas of the substrates are presented. Redox potentials (E°)ofT1 coppers of the laccases and...

Ngày tải lên: 14/02/2014, 18:20