Tài liệu Báo cáo khoa học: Amprenavir complexes with HIV-1 protease and its drug-resistant mutants altering hydrophobic clusters docx
Tài liệu Báo cáo khoa học: Amprenavir complexes with HIV-1 protease and its drug-resistant mutants altering hydrophobic clusters docx
... authors.
HIV protease mutants altering hydrophobic clusters C-H. Shen et al.
3714 FEBS Journal 277 (2010) 3699–3714 ª 2010 The Authors Journal compilation ª 2010 FEBS
Amprenavir complexes with HIV-1 protease ... PR
V32I
,PR
I50V
,PR
I54M
,PR
I54V
,PR
I84V
and
PR
L90M
complexes with APV.
Table S1. Hydrogen bond interactions between HIV-1
protease and APV.
Table...
Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
... interactions of cystatin F with several
of its potential targets, cathepsins F, K, V, S, H, X and C, were studied
in vitro. Cystatin F tightly inhibited cathepsins F, K and V with K
i
values
ranging ... cystatin F was compared with that of
the cathepsins, cystatin F was found to be colocalized
with cathepsins X and H (Fig. 2C,D), but not with
cathepsins L (Fig. 3A), B, C an...
Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... staining
with Coomassie blue showed single bands for the
wild-type and mutants D392N and E315M, migrating
with an M
r
of 63 000 (Fig. 4A). In the case of the
E315Q mutant, an additional faint band ... chitin
polymer was incubated with the mutants E315Q and
E315M, even after 60 min. On the other hand, the
D392N mutant was able to hydrolyze chitin with
20% residual activit...
Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx
... loss-of-function
of BTK and ITK and the gain-of-function fusions ITK–SYK
and BTK–SYK
Alamdar Hussain
1,2,
*, Liang Yu
1,3,
*, Rani Faryal
1,2
, Dara K. Mohammad
1
, Abdalla J. Mohamed
1,4
and C. I. Edvard ... [26,27], and
therefore we will only review this topic very briefly.
Patients with XLA have a differentiation block result-
ing in an almost complete absence of B lymphocytes...
Tài liệu Báo cáo khoa học: a-enolase: a promising therapeutic and diagnostic tumor target ppt
... mam-
mals, and its sequence is highly conserved [3]. In mam-
mals, three genes, ENO1, ENO2 and ENO3 encode for
three isoforms of the enzyme, a-enolase (ENOA),
c-enolase and b-enolase, respectively, with ... expression is correlated with
greater tumor size, poor nodal status and a shorter dis-
ease-free interval [78]. In head and neck and nonsmall
cell lung cancer, patients...
Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx
... spec-
trometer with an inverse broadband probe installed with
z-gradient. The 1D spectra
1
H and
13
C; the homonuclear
2D spectra DQF-COSY, TOCSY, NOESY and ROESY;
the
1
H–
3
C HSQC and HMBC; and the
1
H–
15
N ... 6084 g and 4 °C for 30 min. The supernatant was sep-
arated from the cell pellet and incubated with XAD16
resin (4.0 gÆL
)1
). The resin was collected by filtration...
Tài liệu Báo cáo khoa học: Paradoxical interactions between modifiers and elastase-2 Patricia Schenker and Antonio Baici docx
... poly-
disperse, and the chondroitin sulfates exist as co-poly-
mers of the 4- and 6-sulfate isomers (Ch4S, Ch6S) with
various compositions and mean molecular masses that
depend on animal species and tissue. ... larger mean molecular mass,
with about 130 disaccharide units per chain, compared
with only 46 for the 4-isomer (Table 1), and more favor-
able electrostatic interactio...
Tài liệu Báo cáo khoa học: How disorder influences order and vice versa – mutual effects in fusion proteins containing an intrinsically disordered and a globular protein docx
... PNT and SIC1 share a high con-
tent of unordered stretches (50–59%) and a low con-
tent in a-helices and b-strands (2–3% and 22–29%,
respectively), whereas GFP is rich in b-strands (46%)
and ... and compactness and investigated the
properties of these fusion proteins using biochemical
and biophysical methods. GFP is a globular protein
with a stable fold and known 3D struct...
Tài liệu Báo cáo khoa học: The heat shock factor family and adaptation to proteotoxic stress pdf
... HSF1 and a second HSF gene, HSF2,
were isolated by screening a library with degenerate
oligonucleotide probes [20,21], and the mouse HSF1
and HSF2 genes were isolated by cross-hybridization
with ... develop-
mental processes such as gametogenesis and neuro-
genesis, in the maintenance of sensory and ciliated
tissues, and in immune responses [12–14]. HSF1- and
HSF3-mediated m...
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ADR), the 2008 cell line and its sublines over-expressing
MRP1, MRP2 or MRP3, and the cell line HEK293 and its sublines over-expressing ... continuously with an excitation wavelength of 555 nm and an emission wavelength of
575 nm. Inhibition of the NBD-Cl-sensitive efflux allowed the uptake of TMRM and its qu...