Tài liệu Báo cáo khoa học: The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity docx

... Journal compilation ª 2010 FEBS 3189 The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity Andre ´ T. Fernandes 1 , Joa ˜ o M. Damas 1 , Smilja ... transfer; DSC, differential scanning calorimetry; MCO, multicopper oxidase; McoP, multicopper oxidase from Pyrobaculum aerophilum ;N 2 OR, nitrous oxide...

Ngày tải lên: 18/02/2014, 04:20

... full- length DAPK-1, although the effect was weaker (Fig. 4B). Given the biological activity of s-DAPK-1 in the membrane-blebbing assay, we evaluated the activity of the mutant with the tail deletion (Flag-TD; ... supporting the existence of a protease that cleaves s-DAPK-1 protein in vivo. The reason why the cleavage band was not observed in this assay may be the rapi...

Ngày tải lên: 18/02/2014, 17:20

... and other proteins involved at the biochemical level. Such a situation now exists for dis- covery of genes involved in PUFA biosynthesis follow- ing the completion of the genome sequence of the ... in the TpdesO gene. The amino acid sequence of PtDEL5 and TpDESO exhibited 63% identity, sug- gesting that TpDESO could catalyse the D 5 -desatura- tion step in the PUFA biosyntheti...

Ngày tải lên: 20/02/2014, 01:20

... thetase to esterify the 13cROL generated in the reac- tion. Therefore, 13cROL is accumulated in the absence of LRAT in the reaction (Fig. S3B). Under the in vitro assay conditions of the ... the entire thickness of the inner retina. The Mu ¨ ller cell constitutes an ana- tomical link between the retinal neurones and supports their activities by exchanging molecules between...

Ngày tải lên: 14/02/2014, 14:20

... mitochondria during apoptosis. They induce the permeabilization of the mitochondrial membrane with the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic protein Bax ... mitochondria and then translocate to the nucleus where they induce DNA fragmentation in a caspase-independent manner. Whether apoptosis is induced or not depends on the a...

Ngày tải lên: 14/02/2014, 22:20

... enthalpy is the sum of two terms: the reaction enthalpy, independent of the buffer used in the experiment, and another term representing the contribution of the proton ionization of the buffer, which ... HCl, 1 mM MgCl 2 , 100 mM NaCl. The upper panels show the raw data for the heat effect during the titrations; the lower panels are the binding isotherms. Table 2. Th...

Ngày tải lên: 16/02/2014, 14:20

... signal within the GBD part of the protein. Another possible explanation for the observed autoinhibitory activity is that the Hth domain mediates some intramolecular interaction, or affects the confor- mation ... 1D). To further define the Meis2d transcriptional AD, we tested two other GBD fusion proteins, which contained either the Meis2 HD and C-terminal region, or just the...

Ngày tải lên: 16/02/2014, 15:20

... A from the com- plex with cathepsin H are shown in cyan (PDB code: 1NB3) [28]. The two structures of stefin A from the complex with cathepsin B are shown in red. The stefin B structure from the ... residues, A49 from the tip of the first binding loop and L73 from the second binding loop, fill the places that the two histi- dines occupy in the native structure. Besi...

Ngày tải lên: 18/02/2014, 04:20

... Val24 at the membrane–water inter- face, the other with Lys28 at the interface [4,5]. The location of the peptide within the membrane may affect the types of interactions that it has with the surrounding ... distance from the protein, for simulation A1. The results are indicative of all the other simulations involving Ab, and thus are representative of the general co...

Ngày tải lên: 18/02/2014, 08:20

... 296(154) [14–17]. The high specific activity of these FVIIa variants is presumably linked to a more stable burial of the N-terminus of the protease domain in the activation pocket of the activation ... were based on the results of the titra- tion experiment. Enzymatic activity, inhibitor reactivity and sTF binding of G372A-FVIIa The very slow auto-activation of G372A-FVII, o...

Ngày tải lên: 18/02/2014, 08:20