Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx

... compilation ª 2010 FEBS The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium Sukaina Zeitoun-Ghandour1, John M. Charnock2, ... predominantlyS-bound and zinc is predominantly O-bound.C. elegans metallothioneins are not the onlyplayers in metal detoxiﬁcation and homeostasis The effects on the ligand environments of cadmium and zinc ... C. elegans. In contrast, the reduced accumulation, or excretion, of cadmium and zinc is MT-mediated, as there was a large effect on the levels of accumulated zinc and cadmium when CeMT-1and...

Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

... this sideof the enzyme is optimized for interacting with the longer (polymeric) part of the substrate. In conclusion,these experimental data and the inferences made from the sequence and structural ... [17,20] and CHB1 [23]. It is conceivable that the above-mentioned two mutations in the binding sur-face and the two insertions that are putatively close tothis surface (Fig. 3) endorse LlCBP33A and ... for binding tochitin and for the positive effect on chitinase efﬁciency[10,17] (Figs 2 and 3). Comparison of the LlCBP33A and CBP21 sequences shows two substitutions in the conserved surface...

Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... pH(6.0–8.0) and at low temperatures (0–35 °C). We focuson the function and relationship of the two singledomains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain ... (Fig. S1). There are two pairs ofcysteine residues, Cys142 and Cys193 in PhyH-DII and Cys445 and Cys495 in PhyH-DI. These residuesare at the same positions as Cys157, Cys206, Cys450 and Cys498 ... with other phytases to release 1.2–2.5-fold phosphate. This is the ﬁrst time the substratespeciﬁcities and functions of the two domains havebeen characterized in a tandemly repeated BPP. The same...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... positioned these kinases as new downstreamOXI1 components and named the proteins PTI1-1, 1-2 and 1-3. One of the prey cDNA encoded PTI1-1(At1g06700) and a second prey cDNA encodedanother member of the ... indicatedtime points. The mixes were then incubated with [c-32P]-ATP and MBP (10 l g) for 30 min. The top panel shows the kinase assayvisualized by autoradiography and the bottom panel shows the Coo-massie ... bothOXI1 and PTI1-4 expression patterns partially overlap. The two genes are strongly expressed in the root and root hairs and are induced upon oxidative stress treat-ments. These ﬁndings strengthen...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... together with the E2F site [20,21]. The DRS ⁄ CHR in the B-Myb pro-moter deviates most from other CHR sequences withits two- nucleotide exchange from the CHR consensus(Fig. 2). Changing the ... (Fig. 2).NF-Y is the main activator, and the distance between two CCAAT-boxes is31, 32, or 33 bpAlready with the discovery of the ﬁrst CDE ⁄ CHRgenes it was recognized that these promoters ... Caenor-habditis elegans synMuvB proteins and is composed ofp130, E2F4 ⁄ 5 and DP1 ⁄ 2 and a module containing the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatin-associated Lin-9 and Lin-53 ⁄...

Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... Maintaining the balance between cell survival and apoptosis is critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direction results in eitherKeywordsapoptosis; ... reagents and the recombinant humanTRAIL ⁄ APO 2 ligand were purchased from Invitrogen and Feldan Bio (St-Laurent, QC, Canada), respectively. The caspase 3 substrate (Ac-DEVD-pNA) and the inhibitorsubstrate ... [16,17]. The BH3domain-only proteins are a subfamily of the Bcl-2family involved in the initiation of apoptosis through the mitochondrial pathway. The key event in the mito-chondrial pathway is the...

Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

... of the ARE I-AUUUA and ARE III-AUUUAsequences used primers SJS172 and SJS173, and SJS174 and SJS175, respectively. Mutation of the atypical AU-richsequence used primers SJS259 and SJS260, and ... SJS260, and the creationof the ARE II ⁄ ARE IV double mutant used primers SJS261 and SJS262. The mutagenesis of ARE I and III introducedHindIII restriction sites and so the creation of the pTET-GLO3¢ ... diagrammatic representation of the PAI-2 3¢ UTR showing the location and sequence of the AU-rich regions of interest within the ‘extended ARE’, and the sequences of the various ‘extended ARE’mutants...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... isolated and analyzed by northern blot using Fluc and Rluc riboprobes. The radioactive signals were quantiﬁed by the STORM 820 equipment and IMAGEQUANT software (Molecular Dynamics). The Rluc ... [15]. On the other hand, other studies indicated that ASF ⁄ SF2activates the translation of mRNAs bearing ASF ⁄ SF2binding sites within the coding region in intact cells[16,54]. The capacity ... between the nucleus and the cytoplasm. Genes Dev 12, 55–66.31 Gui JF, Tronche`re H, Chandler SD & Fu XD (1994)Puriﬁcation and characterization of a kinase speciﬁc for the serine- and arginine-rich...

Tài liệu Báo cáo khoa học: The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity docx

... loop reﬁnement, the ﬁnal model presented 89.1% of the residues in the mostfavored regions of the Ramachandran plot, 10.9% in the additional allowed regions, and no residues in the gener-ously ... bridging ligand and therefore EPR silent, show a characteristic absorptionband at 330 nm. The T2 site lacks strong absorptionbands, and exhibits a large parallel hyperﬁne splittingin the EPR spectra ... to the space between the cytoplasmic membrane and the external protein surfacelayer [19]. The mcop gene encodes a protein with 477amino acids and a predicted molecular mass of52.9 kDa. The...

Tài liệu Báo cáo khoa học: The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress docx

... highly similar to that of IscS(Fig. 1A), with a two- domain organization and the presence of both a-helices and b-strands. On the basisof these data and the fact that alr2505-expression isspeciﬁcally ... toapoferredoxin, and the ﬁnding that slr0387 and sll0704mutants were obtained suggested that none of themwas essential for the viability of this cyanobacterium[24]. The cellular targets to which these ... (http://www.cgl.ucsf.edu/chimera/download.html) [53]. The tertiary structure of IscS monomer was predictedusing the PHYRE server. The two structures were then superposed. The IscS monomer is shown in red and the OsiS monomer in...

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