Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx

... compilation ª 2010 FEBS The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium Sukaina Zeitoun-Ghandour 1 , John M. Charnock 2 , ... predominantly S-bound and zinc is predominantly O-bound. C. elegans metallothioneins are not the only players in metal detoxification and homeostasis T...

Ngày tải lên: 16/02/2014, 15:20

... this side of the enzyme is optimized for interacting with the longer (polymeric) part of the substrate. In conclusion, these experimental data and the inferences made from the sequence and structural ... [17,20] and CHB1 [23]. It is conceivable that the above-mentioned two mutations in the binding sur- face and the two insertions that are putatively close to this s...

Ngày tải lên: 18/02/2014, 08:20

... pH (6.0–8.0) and at low temperatures (0–35 °C). We focus on the function and relationship of the two single domains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain ... (Fig. S1). There are two pairs of cysteine residues, Cys142 and Cys193 in PhyH-DII and Cys445 and Cys495 in PhyH-DI. These residues are at the same positio...

Ngày tải lên: 14/02/2014, 15:20

... positioned these kinases as new downstream OXI1 components and named the proteins PTI1-1, 1-2 and 1-3. One of the prey cDNA encoded PTI1-1 (At1g06700) and a second prey cDNA encoded another member of the ... indicated time points. The mixes were then incubated with [c- 32 P]-ATP and MBP (10 l g) for 30 min. The top panel shows the kinase assay visualized by autoradiography...

Ngày tải lên: 14/02/2014, 19:20

... together with the E2F site [20,21]. The DRS ⁄ CHR in the B-Myb pro- moter deviates most from other CHR sequences with its two- nucleotide exchange from the CHR consensus (Fig. 2). Changing the ... (Fig. 2). NF-Y is the main activator, and the distance between two CCAAT-boxes is 31, 32, or 33 bp Already with the discovery of the first CDE ⁄ CHR genes it was recognized t...

Ngày tải lên: 16/02/2014, 09:20

... Maintaining the balance between cell survival and apoptosis is critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direction results in either Keywords apoptosis; ... reagents and the recombinant human TRAIL ⁄ APO 2 ligand were purchased from Invitrogen and Feldan Bio (St-Laurent, QC, Canada), respectively. The caspase 3 substra...

Ngày tải lên: 16/02/2014, 09:20

... of the ARE I-AUUUA and ARE III-AUUUA sequences used primers SJS172 and SJS173, and SJS174 and SJS175, respectively. Mutation of the atypical AU-rich sequence used primers SJS259 and SJS260, and ... SJS260, and the creation of the ARE II ⁄ ARE IV double mutant used primers SJS261 and SJS262. The mutagenesis of ARE I and III introduced HindIII restriction sites and...

Ngày tải lên: 16/02/2014, 09:20

... isolated and analyzed by northern blot using Fluc and Rluc riboprobes. The radioactive signals were quantified by the STORM 820 equipment and IMAGEQUANT software (Molecular Dynamics). The Rluc ... [15]. On the other hand, other studies indicated that ASF ⁄ SF2 activates the translation of mRNAs bearing ASF ⁄ SF2 binding sites within the coding region in intact cells [16,54]....

Ngày tải lên: 16/02/2014, 15:20

... loop refinement, the final model presented 89.1% of the residues in the most favored regions of the Ramachandran plot, 10.9% in the additional allowed regions, and no residues in the gener- ously ... bridging ligand and therefore EPR silent, show a characteristic absorption band at 330 nm. The T2 site lacks strong absorption bands, and exhibits a large parallel hyperfine spli...

Ngày tải lên: 18/02/2014, 04:20

... highly similar to that of IscS (Fig. 1A), with a two- domain organization and the presence of both a-helices and b-strands. On the basis of these data and the fact that alr2505-expression is specifically ... to apoferredoxin, and the finding that slr0387 and sll0704 mutants were obtained suggested that none of them was essential for the viability of this cyanobacterium [2...

Ngày tải lên: 18/02/2014, 04:20