Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx

... thatincludes a DNA-binding domain, a ligand-bindingdomain and a transactivation domain. The DNA-bind-ing domain is responsible for DNA binding speciﬁcity and dimerization, and the ligand-binding ... ª 2010 FEBSMINIREVIEW Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing Khairul I. Ansari and Subhrangsu S. MandalDepartment of Chemistry and Biochemistry, ... steroid- hormone- mediated gene activation and signaling. In this minireview, wesummarize recent advances in understanding the roles of MLLs in gene regulation and hormone signaling and highlight...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf

... proteins. Thus, the MEN1 ⁄ LEDGF-interactingdomain linked to DNA-binding domains (AT-hook and MT domain) becomes disconnected from the PHDdomains, the FYRN domain, the transactivatingdomain, ... different signaling pathways,for example, protein kinase C, protein kinase A,RAS ⁄ RAF, WNT-, phosphatidylinositol 3-kinase and Hedgehog, and thus affects metabolism, the cell cycle, gene expression, ... as b-catenin and insulin signaling, as well as apoptosis. Active GSK3blocks HH signaling via SMO, and alsoblocks apoptosis and MYC-mediatedactions. Moreover, it allows clonal growth and stabilizes...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: histone H3 lysine 4 methyltransferases from yeast to human pptx

... clinical trials in epigenetic therapy. Rev Recent Clin Trials 1, 169–182.162 Ansari KI & Mandal SS (2010) Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing. ... (2000) Achromatin remodelling complex involved in transcrip-tion and DNA processing. Nature 406, 541–544.24 Conaway RC & Conaway JW (2009) The INO80 chro-matin remodeling complex in transcription, ... methylation in regulation of chromatin structure and gene expression.As in S. cerevisae, H3K4 methylation in Sch. pombe iscatalyzed by a SET domain-containing protein, Set1.The Sch. pombe Set1 protein...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... S-adenosyl-homocysteine; BD, bromo domain; CBP, CREB-binding protein; CREB, cAMP response element-binding;H3K4, histone H3 lysine 4; HMT, histone methyltransferase; MLL1, mixed lineage leukemia protein-1; ... requiredfor the structural integrity of the protein, as mutation ofany of the cysteine residues involved in zinc coordina-tion results in protein unfolding [22]. The structure con-tains a positively ... complexes.Binding of the MLL1 Win motif to the central argi-nine-binding pocket of WDR5 raises questions aboutthe proposed role of WDR5 in binding histone H3, atleast while WDR5 is incorporated into...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13 docx

... binding proﬁles in a time-dependentmanner in ERE1 and ERE2 (Fig. 5B). In agreementwith the above ﬁndings, binding of ERa and ERb wasincreased in both ERE1 and ERE2 in the presence ofE2. Interestingly, ... Binding of ERa and ERbwas increased in both ERE1 and ERE2 of theHOXC13 promoter (Fig. 5A, lanes 1–4). The levels ofE2-induced binding of ERa and ERb were higher in ERE2 than in ERE1. ERE3 and ... CPF3¢-end processing machinery in RNAP II-dependent gene looping. Genes Dev 19, 2969–2978.46 Singh BN & Hampsey M (2007) A transcription-inde-pendent role for TFIIB in gene looping. Mol...

Tài liệu Báo cáo khoa học: What MAN1 does to the Smads TGFb/BMP signaling and the nuclear envelope Luiza Bengtsson pdf

... Prelamin A and BAF are also bindingpartners of emerin [39]. Interestingly, the N-terminusof human MAN1 binds the human emerin itself(Fig. 1; [8]). Emerin is an integral membrane protein and ... forbinding to emerin in vitro. J Biol Chem 278, 6969–6975.8 Mansharamani M & Wilson KL (2005) Nuclear mem-brane protein MAN1: Direct binding to emerin in vitro and two modes of binding ... domain adopt a stable a ⁄ b-foldfound in several DNA-interacting transcription factors[45]. Indeed, a MAN1 fragment consisting of the WHdomain binds DNA with nanomolar afﬁnity and thebinding...

Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

... con-taining DNA spots (5 lg each) corresponding to inter-nal fragments of ADC or cruzipain genes. The latter(used as a control) is a housekeeping gene that encodesthe main cysteine proteinase ... Analysis of a cDNA-encoding arginine decarboxylase from oat reveals simi-larity to the Escherichia coli arginine decarboxylse and evidence of protein processing. Mol Gen Genet 224,431–436.31 ... to a-amanitin of the transcription of argininedecarboxylase (ADC) and cruzipain genes in transformed Trypano-soma cruzi. Permeable parasites were preincubated at 0 °C for5 min in the absence...

Tài liệu Báo cáo khoa học: Mixed-type noncompetitive inhibition of anthrax lethal factor protease by aminoglycosides ppt

... enzyme–inhib-itor binding interaction.log KðappÞi¼ log KÃþ 1: 18ZEZLﬃﬃIpð7ÞDiscussion In this study we have determined that neomycin B and its close structural analog, neamine, are mixed- typenoncompetitive ... manifes-ted in Figs 3 and 4. Note that the Lineweaver–Burkplot in Fig. 4 is distinctly nonlinear.The results are illustrated in Fig. 5, in which thewhite (open) symbols represent data points taken intothe ... 5 and 1 : 11. This again suggests thatthe inhibitors bind to the enzyme either at two distinctbinding sites, or at the same site but in two differentorientations. For neomycin B, the principal...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... shown that interactions betweenKCs and endotoxin comprise the initiating event lead-ing to hepatotoxicity in liver injury, including endotox-emia and ischemia ⁄ reperfusion injury [14]. In ourstudies, ... factor-a and interleukin-6, and increasedhepatic mRNA levels of tumor necrosis factor-a and interleukin-6 weredecreased by depletion of KCs. Our ﬁndings suggest that ablation of KCsprotects against ... marker gene CD163. In humans and animals, infections or in ammatorystimuli cause changes in the activities and expressionlevels of various forms of CYP in the liver. In mostcases, CYPs and their...

Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx

... of openingof the initial double strand and the annealing of thenew duplex ﬁnally results in either the replacement ofthe original strand or the expulsion of the invadingstrand, according to ... unwinding. High-resolution X-ray struc-tures have given insights into the mechanism(s) ofDEAD-box helicases. The binding sites for double-stranded RNA and ATP overlap, resulting in coupledbinding ... domains [27], CCHH-zinc ﬁngers[28], dsRBDs [29], and PAZ domains [30], the inter-faces are tightly packed and provide perfect comple-mentarity between the binding partners. In contrast,interfaces...

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