Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

... between the EAD and RNA polymer- ase II subunit [11,45]. The interaction between the EAD and RGG3 might inhibit the high-affinity Htelo binding of RGG3. To gain further insight into the induction of ... Htelo (Fig. S6). These findings indicate the importance of the guanidinium group of the Arg in RGG3 for binding to the G-quadruplex. In conclusion, EWS appears...

Ngày tải lên: 15/02/2014, 01:20

... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein inter- action domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger ... FEBS Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the...

Ngày tải lên: 16/02/2014, 09:20

... bacteria. The difference between these two mutants in bacterial clearance might reflect differences in the integrity of their outer membrane, binding activity and sensitivity of the murine comple- ment-mediated ... containing the various agents were plated on the lawn in triplicate at 37 °C for 18 h. Sensitivity was assessed by measuring the diameter of the zone of...

Ngày tải lên: 18/02/2014, 14:20

... representing the 5¢- and 3¢-UTRs are shown in white in the genomic gene and the cDNA. Intron size in bp, domain size in bp and domain size in amino acids are indicated at the bottom of each schematic ... [15]. The locations of the exon–intron junctions in SmSmad1B are shared by Smad genes from other species. For example, the loca- tion of the intron wi...

Ngày tải lên: 18/02/2014, 16:20

... CA protein sequences. Iden- tical and similar amino acids shared by at least 50% of the isoforms are shown in black and grey, respectively. Histidine residues involved in zinc binding in the catalytic ... 94, 96 and 119 in CAII from Homo sapiens) which are directly involved in binding the zinc cofac- tor, are conserved in the two R. pachyptila sequences (positi...

Ngày tải lên: 18/02/2014, 16:20

... number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tubulin myosin and profilin) were differentially expressed in C. ... assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these chaperones c...

Ngày tải lên: 18/02/2014, 16:20

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat ... present in the region encoding the N- terminal portion of the protein (including the signal peptide and small tandem repeats), a large exon com- prises the middle r...

Ngày tải lên: 18/02/2014, 17:20

... [4–6]. In order to improve the biological production method, a detailed understanding of the biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions ... reactions and the genes encoding these enzymes. The biosynthesis of triterpene saponins involves the initial cyclization of 2,3-oxidosqualene, a common pre- curso...

Ngày tải lên: 19/02/2014, 07:20

... in the absence of the STAT2 DNA binding domain as revealed by real-time PCR To more quantitatively examine the expression of IFN- regulated genes in the absence of the STAT2 DNA binding domain, ... Thus, IFN-inducible expression of these genes is impaired in the absence of the STAT2 DNA binding domain, sug- gesting that their IFN-inducible transcription require...

Ngày tải lên: 19/02/2014, 07:20

... indicate the molecular sizes of marker proteins in (A), the 110-, 95- and 75-kDa bands of matriptase in (B), and the 33-kDa, uncleaved form and the 25-kDa, cleaved form of IGFBP-rP1 in (C). The ... under reducing conditions it was split into a major 75-kDa band of the single-chain, latent enzyme and a minor 50 kDa band of the two-chain, active enzyme (Fig...

Ngày tải lên: 19/02/2014, 07:20