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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... between the EAD and RNA polymer-ase II subunit [11,45]. The interaction between the EAD and RGG3 might inhibit the high-affinity Htelobinding of RGG3.To gain further insight into the induction of ... Htelo(Fig. S6). These findings indicate the importance of the guanidinium group of the Arg in RGG3 for binding to the G-quadruplex. In conclusion, EWS appears to be a DNA- and RNA-binding protein that ... DNA-binding motif in nucleolin [21–27]. The RGG domain of FMRP, which is an RNA-binding protein involved in nerve cell differentiation,interacts with the G-quartet forming RNA [22–25]. In addition,...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

... seen in the putative T-boxDNA-binding domain of t-bet, the STAT protein inter-action domain, STAT protein all-alpha domain, STATprotein DNA-binding domain and SH2 domain of stat6, and the zinc-finger ... FEBS Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio Suman Mitra, Ayham Alnabulsi, Chris J. Secombes and ... on the expression of these genes within humans and mice, of T-bet within Ginbuna crucian carp [27] and STAT6 in mandarin fish [28] have shown similar widespreadexpression. In the case of STAT6, ...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... bacteria. The difference betweenthese two mutants in bacterial clearance might reflectdifferences in the integrity of their outer membrane,binding activity and sensitivity of the murine comple-ment-mediated ... containing the various agents were plated on the lawn in triplicate at37 °C for 18 h. Sensitivity was assessed by measuring the diameter of the zone of growth inhibition in two axes, and the ... 24 h of incubation, the resulting Zeorcolonies were selected for PCR identification using primers b1X and b1B, and the inactivated lpxXmutant was verified by sequencing.Cloning of lpxL and construction...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... representing the 5¢- and 3¢-UTRs areshown in white in the genomic gene and the cDNA. Intron size in bp, domain size in bp and domain size in amino acids are indicated at the bottom of each schematic ... [15]. The locations of the exon–intron junctions in SmSmad1B are shared bySmad genes from other species. For example, the loca-tion of the intron within the MH1-encoding region isconserved in the ... the human Smad4 gene, whereas the intron within the linker-encoding region of SmSmad1Bis shared by human Smad3 [16]. The location of the intron within the MH2-encoding region is highly con-served...
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Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... CA protein sequences. Iden-tical and similar amino acids shared by at least 50% of the isoforms are shown in black and grey, respectively. Histidine residues involved in zinc binding in the catalytic ... 94, 96 and 119 in CAII from Homo sapiens)which are directly involved in binding the zinc cofac-tor, are conserved in the two R. pachyptila sequences(positions labeled ‘Z’ in Fig. 3). These ... proteins: one of 27 kDa in the branchialplume, and another of 28 kDa in the trophosome.From the differential expression results we obtained,Fig. 3. Alignment of complete RpCAbr and RpCAtr amino...
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Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx

Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx

... number of genes encoding structural components of the cytoskeleton and proteins involved in contractilefunctions (including actin, tubulin myosin and profilin)were differentially expressed in C. ... assisting the folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu-lation of all of these chaperones confirms the severity of the thermal ... tothermal stress are known to involve major adjustments in the expression of ribosomal genes and genes codingfor proteins involved in RNA metabolism and proteinsynthesis. In fact, protein...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf

Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. SixGST-fused recombinant proteins containing the three distinctivedomains of tandem repeat ... present in the region encoding the N-terminal portion of the protein (including the signalpeptide and small tandem repeats), a large exon com-prises the middle region of the ORF, which encodes the ... proteinsinduce aragonite crystals [4–7]. We therefore exam-ined proteins contained in aggregates within the otolith matrix and identified a protein contained in the HMW protein–glycosaminoglycan...
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... [4–6]. In order to improve the biological production method, adetailed understanding of the biosynthesis of triterpenesaponins is required, including the enzymes catalyzing the sequence of reactions ... reactions and the genes encoding theseenzymes. The biosynthesis of triterpene saponins involves the initial cyclization of 2,3-oxidosqualene, a common pre-cursor of all the sterol and triterpene ... Kirakosyan A, Inoue K, HiraokaN, Ikeshiro Y, Kushiro T, Shibuya M & Ebizuka Y(2001) Cloning and characterization of a cDNA encod-ing b-amyrin synthase involved in glycyrrhizin and soyasaponin biosynthesis...
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Tài liệu Báo cáo khoa học: Identification of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc

Tài liệu Báo cáo khoa học: Identification of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc

... in the absence of the STAT2 DNAbinding domain as revealed by real-time PCRTo more quantitatively examine the expression of IFN-regulated genes in the absence of the STAT2 DNAbinding domain, ... Thus,IFN-inducible expression of these genes is impaired in the absence of the STAT2 DNA binding domain, sug-gesting that their IFN-inducible transcription requiresISGF3-independent STAT2-containing ... expression is IFN-inducible and independent of ISGF3 but highly dependent on the STAT2 DNAbinding domain. This report is the first analysis of the contribution of the STAT2 DNA binding domain to IFN...
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Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc

Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc

... indicate the molecular sizes of marker proteins in (A), the 110-, 95- and 75-kDa bands of matriptase in (B), and the 33-kDa, uncleaved form and the 25-kDa, cleaved form of IGFBP-rP1 in (C). The ... underreducing conditions it was split into a major 75-kDaband of the single-chain, latent enzyme and a minor50 kDa band of the two-chain, active enzyme (Fig. 7C)[10]. Based on the relative band intensity, ... determine the class of the IGFBP-rP1-cleavingproteinase, OVISE cells were cultured in the presence of various proteinase inhibitors and the processing of IGFBP-rP1 was analyzed. As shown in Fig....
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