Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... 4919 Proteomic analysis of dopamine and a- synuclein interplay in a cellular model of Parkinson’s disease pathogenesis Tiziana Alberio 1 , Alessandra Maria Bossi 2 , Alberto Milli 2 , Elisa Parma 1 , ... Statistical analysis, by two-way analysis of β β -Actin α -Synuclein Fig. 1. Relative expression of a- synuclein in b-gal and a- syn cells in r...

Ngày tải lên: 15/02/2014, 01:20

... Gelatinase-mediated migration and invasion of cancer cells. Biochim Biophys Acta 1755, 37–69. 4 Chen JM, Fortunato M, Stevens RA & Barrett AJ (2001) Activation of progelatinase A by mammalian legumain, ... I, Iwata K, Yamashita K & Hayakawa T (1992) Matrix metalloproteinase 9 (92-kDa gelatinase ⁄ type IV collage- nase) from HT1080 human fibrosarcoma cells. Purifica- tion and a...

Ngày tải lên: 18/02/2014, 11:20

... proposals of Dowty, Reape, and Kathol, and to the particular analysis implemented here, is the characterization of nat- ural language syntax in terms of two interrelated but in principle distinct ... advantage of the information that a semantic head provides. For example, a head usually provides information about the remaining daughters that the parser must find,...

Ngày tải lên: 20/02/2014, 18:20

... cell carboxyles- terases and albumin, was controlled by nondenaturing PAGE and activity staining [26,68]. Kinetics of substrate hydrolysis in the absence of ligand Kinetic assays with neutral and ... solution of ATMA was in phosphate buffer, that of o-NAC was in methanol, and those of o-NTFNAC and NATAc were in 50% acetoni- trile. Assays with ATMA were carried out...

Ngày tải lên: 18/02/2014, 17:20

... thylakoids and 1 lm dPQ-treated thylakoids are shown in Fig. 5. E A was unaffected by the addition of dPQ: all data remained in the error bar range for control and dPQ-treated thylakoids for all ... well- distinguished phases in terms of amplitude and half- time (Table 1). In contrast with the I peak observed in FI of intact leaves, the middle step J–I is not usually a...

Ngày tải lên: 19/02/2014, 05:20

... undergoing phase II/III human clinical trials as an antitumor drug. Native onconase from Rana pipiens and its amphibian homologs have an N-ter- minal pyroglutamyl residue that is essential for obtaining fully ... post-translational modifications that can modulate a peptide activity and/ or resistance to degradation, as is the case with acetylation, pyroglutamyl formation or C-terminal ami...

Ngày tải lên: 19/02/2014, 12:20

... lectins s uch as concanavalin A ( ConA), pea lectin, jack fruit (Artocarpus integrifolia) agglutinin (jacalin), snake gourd (Trichosanthes anguina) seed lectin (SGSL) and Trichosanthes cucumerina ... 2,6-toludinylnaphthalenesulfonic acid, auxins and cytokinins, to a variety of p lant lectins [ 40–44] and the binding of H 2 TPPS to human serum albumin and b-lactoglobulin at neu...

Ngày tải lên: 19/02/2014, 16:20

... steel tray. The tray was then placed on top of a hot plate and the temperature maintained at 9 0 °C for 5 min to aid the staining of protein spots. The tray was then placed on a laboratory shaker ... models of neuromuscular diseases are an indispensable tool in modern myology research. They are employed fo r studying the molecular and cellular factors leading to necrotic cha...

Ngày tải lên: 19/02/2014, 16:20

... CAGCAGGATCCTCTAGAGAGTTTAGTCTT TG-3¢)anda5¢-terminus primer (one of 5¢-AAGCT TCACCATGTACCCTGCCCACATGTACCAAGTG TAC-3¢,5¢-AAGCTTCACCATGCCGCACCGG CTC ATCGAGAAAAAGAG-3¢,5¢-AAGCTTCACCATG GCAGTGGTTCTTGAACTTACCTTGAAGC-3¢ or 5¢-AAGCTTCACCATGATTGCCCTGCAGAGTGG TTTACAAGCTG-3¢) ... FLAG-tagged DEC1:2–412 and BMAL1, two sets of primers ( 5¢-AAGC TTGAGCGGATCCCCAGCGCGCAACCACC-3¢ and 5¢-GCAGCAGGATCCTC...

Ngày tải lên: 19/02/2014, 16:20

... CP-pyk (5¢-ACGACTAGTGGATCCATNNNNNAGTTTATTCTT GACANNNNNNNNNNNNNNTGRTATAATNNNNAA GTAATAAAATATTCGGAGGAATTTTGAAATGAATA AACGTGTAAAAATCG-3¢)(N¼ A, T, G, C) and pyk- back (5¢-CTCTACATGCATTTCAACAATAGGGCCTG TC-3¢) ... (5¢-TGGTACTCGAG CAATTTCTGAAGGTATCGAAG-3¢) and pyk2 (5¢-GG AAGGATCCTTGTGTTTTTCTCCTATAATG-3¢) and downstream to pyk using primer pyk3 (5¢-GGAAGGA TCCTTTGTCAATTAATGATCTTAAAAC-3¢) an...

Ngày tải lên: 19/02/2014, 17:20