Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx

... Secondary substrate binding in GH11 xylanases FEBS Journal 278 (2011) 1098–1111 ª 2011 The Authors Journal compilation ª 2011 FEBS 1111 Secondary substrate binding strongly affects activity and binding ... and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases Sven Cuyvers, Emmie Dornez, Mohammad N. Rezaei, Annick Pollet,...

Ngày tải lên: 14/02/2014, 19:20

... efficient binding, complex assembly and enzyme catalysis in vivo. Membrane affinity of a Gla protein often correlates with its membrane localized activity. Strategies used to increase the affinity of ... influence the interaction to the cofactor and thus influence the anticoagulant activity of APC. An influence of position 23 on the interaction to APC substrates FVa and FVIIIa...

Ngày tải lên: 18/02/2014, 06:20

... production of proinflammatory cytokines, to pro- mote the recruitment of dendritic cells and monocytes to the site of injury, and to enhance phagocytosis and the maturation of dendritic cells. All of ... the triggering of an immune response and in the natural process of elimination of activated antigen-presenting cells and termination of the immune response [90]. He...

Ngày tải lên: 18/02/2014, 06:20

... A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. Isomerohydrolase activity of purified ... retinyl ester substrate is essential for the efficient isomerohydrolase activity of RPE65. Kinetics of the isomerohydrolase activity of purified RPE65 To determine the stea...

Ngày tải lên: 18/02/2014, 08:20

... nozzle-skimmer dissociation spectra of 1 : 1, 2 : 1 and 3 : 1 mixtures of 144 and 116 kDa proteins showed the corresponding molecular ions and, for each, 11–17 different mass values of 1–10 kDa that represented ... a molecular mass of 16 309.7 Da, but this matched none of the DNA-predicted proteins. MS ⁄ MS of these ions gave the C-terminal sequence of Fig. 5. These and a...

Ngày tải lên: 18/02/2014, 16:20

... immune and endocrine systems, components of signaling pathways and of the ubiquitin–proteasome pathway, and genes of tumor viruses. Activation of cell-cycle regulators, proto-oncogenes and genes involved ... rate and stability of TATA- binding protein and TFIIB binding. J Biol Chem 276, 6260–6266. 48 Fairley JA, Evans R, Hawkes NA & Roberts SGE (2002) Core promote...

Ngày tải lên: 19/02/2014, 05:20

... site of covalent attachment of FAD and confirmed Trp66 as essential for FAD binding, f or enzyme activity and for the spectral properties o f the wild-type enzyme. A K m of 140 l M and a k cat of ... Biochemistry and Molecular Biology, University of Freiburg, Freiburg, Germany; 2 Laboratory of Biochemistry, University of Groningen, the Netherlands; 3 Institute of Org...

Ngày tải lên: 19/02/2014, 16:20

... are shown as the averages of two individual experiments. Fig. 3. Comparison of affinity for SQAG between the clone-14 and unselected clone. Binding strengths of clone-14 and unselected clone on ... immobilize on the control well. Increase rate of recovery for control ¼ titer of SQAG immobilized well ⁄ titer of control well. Table 1. SPR analysis of the binding of SQAG...

Ngày tải lên: 19/02/2014, 17:20

... model of single stranded nucleic acid binding [25]. (A) Structure of hnRNPA1 RRM 2 in com- plex with single stranded telomeric DNA and scheme of the b-sheet annotated with the conserved RNP 1 and ... contribute to RNA binding, namely RNP 1 positions 1, 3 and 5 and RNP 2 position 2 (see the following section and Fig. 1). The RNP 1 and RNP 2 motifs are located in the centra...

Ngày tải lên: 19/02/2014, 17:20

... yield infor- mation on the specificity of binding of particular vs. bulk lipids, the number of their binding sites and the occupancies and the cooperativity of their potential interactions [1]. ... considered. Microscopic lipid and inhibitor binding constants are derived and allow a close fit to dose–response curves of tadpole narcosis on the basis of a preferential di...

Ngày tải lên: 19/02/2014, 18:20