Tài liệu Báo cáo khoa học: Seeking the determinants of the elusive functions of Sco proteins pptx

... from Sco2 to Sco1 and toward apo-Cox2. Cys-reduced states of Sco1 and Sco2 are also able to exchange Cu(I) between each other. L. Banci et al. Determinants of the elusive functions of Sco proteins FEBS ... Cox17 to the apo-Cu A site passing through Sco1 and Sco2 proteins (Fig. 5), but the molecular details of the role of Sco proteins in the copper ins...

Ngày tải lên: 14/02/2014, 18:20

... thetase to esterify the 13cROL generated in the reac- tion. Therefore, 13cROL is accumulated in the absence of LRAT in the reaction (Fig. S3B). Under the in vitro assay conditions of the ... that the enzy- matic activity of RPE65c is iron-dependent. Characterization of the kinetic parameters of the enzymatic activity of zebrafish RPE65c To determine the steady-s...

Ngày tải lên: 14/02/2014, 14:20

... mice [28]. The instability of DA at the neutral pH of the cytosol causes the forma- tion of toxic aminochromes, and these species are not formed when DA is protonated at the acidic pH of the storage ... counter. Values are the mean of six replicates. The specific uptake was determined by subtracting the uptake counts in the absence of inhibitor from the uptake...

Ngày tải lên: 14/02/2014, 21:20

... observation). In the ternary complex, the histone H3 peptide binds in a deep chan- nel that divides a pair of acidic lobes, one of which is composed of residues from the SET-I region and the other of residues ... structures of other SET domain proteins that also contain the canonical post-SET domain [43–46], formation of the ternary complex results in ordering of...

Ngày tải lên: 16/02/2014, 14:20

... intense interest in the roles of the entire MT family in the brain, with particular focus on the role of these proteins in the injured or neurodegenerative brain. Discovery of GIF AD is a neurodegenerative ... in all of these conditions [22]. In the trau- matically injured brain, however, the synthesis of MT1 and MT2, as well as the synthesis of GIF, are up-regu-...

Ngày tải lên: 16/02/2014, 15:20

... A comparison of the structure of the unli- ganded cathepsin B with the structure of the proenzyme, its complexes with chagasin and stefin A shows that the magnitude of the shift of the occlud- ing ... 1) between stefin A and the different enzymes is the average distance between the centre of mass of CA atoms of the stefin molecule and the centre of mass...

Ngày tải lên: 18/02/2014, 04:20

... 296(154) [14–17]. The high specific activity of these FVIIa variants is presumably linked to a more stable burial of the N-terminus of the protease domain in the activation pocket of the activation ... study of FVIIa [13], and investigated the effects of the mutation on the enzymatic maturation of FVIIa and on the response of FVIIa to TF. Our measurements, in...

Ngày tải lên: 18/02/2014, 08:20

... carrying the respective myc 3 -tagged RIO1- alleles under the control of the GAL10 promoter in the genomic context (the respective alleles were integrated at the URA3 locus with the help of the integration ... suggesting the absence of CK2 sites N-terminal of position 402 in the catalytic domain and the presence of several phosphorylation sites for CK2 in the...

Ngày tải lên: 18/02/2014, 16:20

... reaction of the luciferin ⁄ luciferase mixture, energy donor (ATP) and oxygen results in the emission of light. The intensity of the bioluminescence was detected using a luminometer, and the mitochondrial ... were approved by the University of Cape Town’s Animal Research Ethics Committee, and the investigation conforms to the Guide for the Care and Use of Laborator...

Ngày tải lên: 18/02/2014, 16:20

... inside the hepatocyte to the plasma mem- brane [39], which depends on a ROS-mediated tyrosine phosphorylation of the epidermal growth factor (EGF)-receptor, the association of CD95 with the EGF-receptor, ... summarizes recent progress in the understanding of how osmosensing and osmosignaling integrate into the overall context of growth factor signaling and the executio...

Ngày tải lên: 18/02/2014, 16:20