nghien cuu tach chiet va su dung neng bie: cac thanh phin co hcai tinh sinh hoc caa noc ran, dac biei la cac toxin de su dung bao che -cam xoa bop
dieu tri dau day than k i n h , viem ca, dau k h a p ' K e m toxin: Toxin 0.0 Ig; M a n e n lOOOg kinh m e n , rich loi b a o chi t h u o c tiem tir toxin ^au k h i d a b a o c h e cac che p h a m xoa b o p or kinh m e n , rich loi b a o chi t h u o c tiem tir toxin ^au k h i d a b a o c h e cac che p h a m xoa b o p or tuih sach. toxin, d u n g p h u a n g p h a p d o d o chiu dau b ^ n g lam
n - N g u y e n lieu va p h u a n g p h a p n g h i e n c u u n o n g d a n h gia tac d u n g g i d m dau c u a c a c c h e
p h ^ n a y
^- Nguyen lieu, thiet bi va hod chat
Danh gia tac dung g i a m dau c u a k e m toxin.
a - N g u y e n lieu . , . , . . , ị - ^ - u • - , - j N g u y e n tac c u a p n u o n g p h a p d a n h gia tac d u n g N g u y e n tac c u a p n u o n g p h a p d a n h gia tac d u n g
^ N o c rdn hC m a n g (Naja naja) d o n g kho Mang ^ ^ ^ ^^^^ ^ ^ 3 ^^.^ c a ché tie c h e than kinh t n i n g o n g 5 g) m u a a lang n g h e n u o i ran Vmh San, i^^mg (co tac d u n g g i a m dau kieu m o r r h m ) [8 J o n g 5 g) m u a a lang n g h e n u o i ran Vmh San, i^^mg (co tac d u n g g i a m dau kieu m o r r h m ) [8 J
h u y e n Vinh T u o n a , tinh Vinh Phuc. r ^ ^ , . •- - / ^ -
D a n h gia tac dung g i a m dau d u a c thuc hien tren
2 - Cac phuang phap nghien ciai m a y d o dau t a m n6ng"cua hang U g o - Basile
a - Phuong phap n g h i e n cuu x a c d m h prctem ELỊ K e t q u a v a b a n l u a n
- P h u a n g phap x a c d m h protem / - Phan tdch cac thanh phan co hoal tinh sinh Ham luang protem trong cac m^u noc ran va ^'^^ ^^^ ^^^^ ^^^ ^^^'i col sac kv vai Superdex-75 Ham luang protem trong cac m^u noc ran va ^'^^ ^^^ ^^^^ ^^^ ^^^'i col sac kv vai Superdex-75 dung dich^ d u o c x a c d m h bang phuang pnap Tren ca so n h u n g 1-cet o u a ve chiet lacn lam sach
quang pho h a p thu cue dai c u a dung dich c a c thanh phan trong noc ran_ ho m a n g bang cac protem 6 cac b u a c song 2S0 n m d u o c d o tren p h u a n g p h a p khac nhau, chiing toi x a > " d u n i r u u y protem 6 cac b u a c song 2S0 n m d u o c d o tren p h u a n g p h a p khac nhau, chiing toi x a > " d u n i r u u y m a y quang pho tu n g o a i LẤieOl Shimazụ sau trinh tach n e n g biet timg thanh - h a n c:; hoâ linh do h a m luang protem d u a c xac dinh iheo bica s^j^h hoc lii nóc ran voi m u c dich ;hu d u o c nieu ••^" [^1- q u a cao ahaị Do dat d u r e m u c JIL." n a \ . ^huniz - Phucmg phap tacn cac pnan doan prcfcx; '^^' ^^ ^^^ ^"^^^ SuperGex-:-^ lam ..lat mang . n o
cot sac kv 6 0 x l C m m duoc can baniz boi d u n " d;ch
Tach cac phan dc:an prciem d u o c ticn hann j ^ ^ ^,^f^^_ ^^^ ^^ ^uacW^n hanh k-n m a v ' : ? L C
boi cac pnuong phap sac ky nhu sac ky ;rao doi f p i ^ h Periormance L:ou:d Chrcmaic^rarnv Lon ircn cot v6i C \ [ - va DEAiZ-c^iluiosc. sac Lon ircn cot v6i C \ [ - va DEAiZ-c^iluiosc. sac
kv saniz loc phan tu tren coi voi Seonoex G-T5 , ^^'- " ^ " ^ . P " ^ ^-^ ^''^ ^ ^ '^^^ ^ ^ ^ ' ^ ^^'
superdcx-75 va sac kv hao phu tren 2ei Ca-P '°r^'"' '™"§ ' " " ^ ^"= " S " - ^ ^^'" ^^'^^ - " - ' ^ •<>'
^ ' ' - tjcu duac kicm ira ham luang proicji c ITJnh I j.
n - Phuang phap thu hoai tinh smh hoc Dua vao do thi proiem cho phep ihu cac phan TT • i_ u u • , ^.; - tk'.r.^ nh-.n T-^,-^ n doan chiia cac thanh chan co hoai ::r.n iinh hoc TT • i_ u u • , ^.; - tk'.r.^ nh-.n T-^,-^ n doan chiia cac thanh chan co hoai ::r.n iinh hoc Hoat tmh sinh hoc cua cac tnann pnan tacn ra ^ ^
tir npc ran hO mang duoc tien hinh theo phuang *^^^^ ^ ^ ^ • phap Vanden Bergher va Vlietmck (";. tai phap Vanden Bergher va Vlietmck (";. tai
phong Sinh hoc thuc nghiem, Vien Hoa hoc cdc o,4 -htTp chat Tliien nhien, Vien Khoa hoc va Cong htTp chat Tliien nhien, Vien Khoa hoc va Cong
nshe Viet Nam. I c - Phuong phap thu duac ly cua cac che pnam § J : - ,|^ «^. *• ^ c - Phuong phap thu duac ly cua cac che pnam § J : - ,|^ «^. *• ^ xoa bop tir toxm
Phuons phao bao che va thu duoc ly caa cac che phiim'xoa bop tu toxm duoc tien hann tai che phiim'xoa bop tu toxm duoc tien hann tai Khoa duac ly, Vien Duac lieu Bo y tẹ
- Cong thuc b a o c h e k e m xoa bop: ^_^^ ^^ ^^^^^ ^^^ ^ .^ ^^^^^ ^^^^^^^ ,^^^^ ^^^ ^^^^ ^^^^^
Dd so sanh tac d u n g giam dau cua toxm, ^j^- .^y
chiing toi bao c h e 2 Icai k e m . Mot loai tir toxin ị^.^oauciease; 2-?hosphoiipase; 3-Proieaie; -UTjxm tmh sach, m o t loai tir n o c ran va tinh toan sao , ^ . .. . . , . ; / tmh sach, m o t loai tir n o c ran va tinh toan sao , ^ . .. . . , . ; /
"•^ ..T^ „ ^,^r r-in"' Tuong - - Xuc dinh trorvj luan'' pnan tu cua cac ilianii
cho l u a n e t o x m t r o n e K e m noc ran luuuu ^ ^ ^ ^ ^ K
• . , . ^. ••k'^rn T n v n " pnan iron''noc ran no man^
d u a n g voi luang t o x m trcng K e m toxm K ^
K e m n o c rdn: N o c ran d o n e kho 0.02g; m o Kích thuoc phan tir c i a cac 'iiann m a n trong
• ^^^f^(^r. HOC ran ho m a n e d u o c xac dinh dua vao cac
nen l u u u g .
,J - • —
0 iO 10 :o •iC 5 0 •:0 •u
"0
Marker chu^n: Ribonuclease A (dimer, 27,4 kDa), Trypsin (24 kDa), Trypsin Inhibitor kDa), Trypsin (24 kDa), Trypsin Inhibitor
(20kDa) va Insulin (5,75 kDa). Ket qua sic ky
cac Marker chuin duoc trinh hav o hinh 2.
Hinh 2. D6 ihi chuan de xac dmh trong luong phan
tir cac che phdm.
Markers: 1- Ribonuclease Ạ 2- Trypsir^ 3- Trypsm Inhibitor, 4- Insulin Inhibitor, 4- Insulin
Tir cac gia tn trong luang phan tir cua cac Marker chuan. xay dung do thi chuan de xac Marker chuan. xay dung do thi chuan de xac dinh trong luang phan tir cac che pham trong noc ran ho manẹ Ket aua cho thav toxin co
trong luong phan tir chimg 6-7 kDạ Ket qua nav
phu hap voi ket qua cua mot so tac gia da cong bo tnxac day ve toxin tir noc ran ho mang Naja naja tnxac day ve toxin tir noc ran ho mang Naja naja
Viet Nam [9]
Ket qua khao sat hoat tinh smh hoc cac che phim trong noc r ^ h6 mang cho ihay cung nhu phim trong noc r ^ h6 mang cho ihay cung nhu phospholipase, toxm co hoat tinh khang vi smh vat kiem dinh (bang 1).
Bang 1. Hoal linh khang vi smn vat kâm dmh ciia cdc che phaim tir noc ran hO mang che phaim tir noc ran hO mang
No Mau
Nong do (iz cne toi thieu MIC, ugymj
Vi khuan Qr{-: Nam men j
P S ' £. coil ' ' ' C albicansi £. coil ' ' ' C albicansi
Berjc:rass cer^vtsiae
1 1 PhosDHoiiDase 50 [-1 Toxin L', I Toxin L', I
3 - Sudung toxin tinh sach tic noc rdn de san xuat kem xoa bop
Bang 2. St) sanh tac dung duac ly ciia che pham xoa bop tu loxm VCTI che pham \ca hop \u noc ran
(T= Thai ;:ian chiu dau ciia chuol )
Lo thi nghiem % Uing so voi To
Tj I giay)
% uing so voi To
Doi chirng uang
Q = 18
Doi chung aen D= 19 D= 19 Mo noc ran Mo I ox in : i , 3 r 1.: 23.1 r 2 . 1 Tang 8,4'^c P-.*-> 0,05 Tang 4.:^c ?*.**-> 0.0: 21.5 = 2.0 Giam 5.3'^c P-,">0,05 23.1 =2.2 Tang l.S'~c >*,"""> 0.0: ,3-1 = 1-5 25-4=1.7 Tang 9.8^0 ?*.•"*> 0.05 29.6=3.0 Tang 28.4f-c P*.-""*=0.05 13.8 = 1.: 37.5=59 Tang 51,6% PV"<0.05 45.2 = 9. 2 Tang 90.2% ?*,***< 0.05 23.0 = 2,6 Tang S.Q^c '.*•-"> 0.05 23.3 = 2.6 Tan 2 2,6^ '>0.'J: 28.5=3.0 Tano 23.5^ ?*.•""•> 0.05 49.5=9,9 Tang 108,1^^0 ? • - • " < 0.05
Di nang cao hieu qua sir dung ciia noc ran,y^goai da bang toxm tmh sach tach tir noc ran ho
chiine toi thir nehiem san xuat kem xoa bop mang Naja naja cimg voi '^ duoc tan trong nudẹ
lay ten la "Natox". Ket qua khao nghiem dixac ly IV - Ket luan caa kem xoa bop "Naiox" dupe trinh bay irang caa kem xoa bop "Naiox" dupe trinh bay irang
bang 2, cho thay: