xylanase assay using the somogyi nelson method 120

... determinate the annual gamma dose in thermoluminesccence dating In the consistency with the experimental results it was shown that the more accurate the beta dose evaluation is, the more reliable is the ... of thermoluminecence dating [5,6] Fig the measurement of the gamma dose rate using the radiometer CΡΠ-68-01 This paper presents a new method to evaluate the annual beta dose (Dβ)by measuring the ... 0.073 2.283 ± 0.073 The equivalent dose (Q) was evaluated applying the additive dose method: The thermoluminescence intensities of the samples were measured after the exposure to the radiation from...

Ngày tải lên: 13/02/2014, 04:20

... As the medium contains serum, these spectral changes can be attributed to the interaction of the various proteins present with the QD surface These interactions not lead to the degradation of the ... to the removal of water molecules Following on, we can now see the weight loss due to the removal of the organic molecules from the QD surface We can see a clear difference in the profiles of the ... alter the physical structure of the QDs Consequently, as can be seen from the resulting QY’s, the gelatine must act solely as a co-capping agent for the protection of the QD surface and the reduction...

Ngày tải lên: 11/08/2014, 00:22

... the reference and test diets, the feces collection was started The feces were collected for 10 days from each tank For each diet, fecal samples were collected daily and frozen At the end of the ... at the end of the digestive process (Sullivan and Reigh, 1995) Up to now, only few feed ingredients currently used for grass carp in Vietnam have been assessed for their digest value Thus, the ... stored at 4°C Table shows the ingredients used in the reference and three test diets The digestibility experiment was carried out with three replicates per treatment in a thermoregulated water system...

Ngày tải lên: 28/08/2013, 09:14

... with the existing Japanese Standard Methods for the Examination of Water We therefore suggest that the SPE-derivatization-GC/MS method should be considered for the Japanese Standard Methods for the ... Examination of Water as an EDTA analytical method The next stage to add this new SPE-derivatization-GC/MS method to the Japanese Standard Methods for the Examintion of Water would be to perform ... Japan’s recommended method of EDTA determination by using gas chromatography-mass spectrometry, we focused on the pretreatment of EDTA by using solid-phase extraction in the present study In...

Ngày tải lên: 05/09/2013, 10:15

... structural components The force (and therefore the amplitude of the vibrations) is proportional to the square of the current, and the radiated sound power is proportional to the square of the vibrational ... principally on the ratio of the sound absorption area of the test room, A, to the area of the measurement surface, S The calculated magnitude of K does not depend strongly on the location of the test ... synchronous In these cases, the use of this method is not appropriate The attenuation of the ambient noise, N, depends on the number of averages, n, that are included in the measurement The signal-to-noise...

Ngày tải lên: 25/12/2013, 10:36

... sulphur dioxide and other greenhouse gases • carbon dioxide traps heat in the atmosphere and causes the temperature of the earth to rise • This leads to disruption of the weather patterns eg drought, ... that will damage crops • They also kill harmless insects or can get washed into rivers and pollute the water • They may even end up in the food chain In the 1960s, DDT in the food chain threatened ... added and it then rains, the fertiliser finds its way into rivers and lakes • This causes the water plants to grow and as there is competition for light, some will die • Bacteria decay the dead plants...

Ngày tải lên: 26/01/2014, 10:20

... directly from the data, using an inductive method to identify the themes and subthemes that emerged To ensure rigour and reliability of analysis, the first three transcripts were coded in their entirety ... out the advantages of their implementation in improving services [24,55,56] On the other hand, Ritchie and Dale suggest the existence of some obstacles to implementing these initiatives within the ... to the management, employment conditions, relationships between peers and the organisational role in the community will be more prone to improve the quality of the PA programme; therefore, the...

Ngày tải lên: 14/02/2014, 06:20

... is the seal between the stem and housing Place the probe at the interface where the stem exits the packing gland and sample the stem circumference Also, place the probe at the interface of the ... at the outer surface of the pump or compressor shaft and seal interface If the source is a rotating shaft, position the probe inlet within cm of the shaft-seal interface for the survey If the ... at approximately the center of the exhaust area to the atmosphere 8.3.1.5 Process Drains For open drains, place the probe inlet at approximately the center of the area open to the atmosphere For...

Ngày tải lên: 14/02/2014, 08:20

... Table 1, the demographic statistics displayed there are fairly selfexplanatory; I discuss their relevance in the following Section Turning to the career statistics, the average actor in the sample ... in the film industry live either in southern California or in New York All four Senators and a majority of the U.S Representatives from these states are Democrats; therefore, to access power there, ... I estimate the determinants of contributions using data on the 865 actors in the sample Coefficients significant at the 10% level are indicated in bold The first three columns use the indicator...

Ngày tải lên: 19/02/2014, 10:20

... termination of the degradation of the enzyme [12], because the inhibition of the reaction also blocks the formation of free radicals [28] Thus, the inactivation of the enzyme can be ascribed to the presence ... the origin of the phenomenon The reason is of course that the changes in concentrations are inherent to the reaction system (i.e they depend on the entire network of elementary reactions) rather ... crucial role in the dynamics of the peroxidaseoxidase reaction [13], and therefore they not appear in most detailed models of the reaction The pronounced dependence of the stability of the enzyme...

Ngày tải lên: 20/02/2014, 11:20

... to binding by analyzing the surface of the residues covered by the protein They also see an interaction of the C-terminal part RPAP with the surface of the antibody In the shorter peptide that ... less to the ligand The C-terminal part of the peptide (RPAP) has close contacts to the next protein in the crystal cell and is therefore stabilized on the surface of SM3 It is unclear whether this ... caused by the GalNAc residue and/or by the binding contribution from the GalNAc residue Docking the glycopeptide to the antibody the sugar ring has little contact to the protein surface Only the N-acetyl...

Ngày tải lên: 21/02/2014, 15:20

... the reference and test diets, the feces collection was started The feces were collected for 10 days from each tank For each diet, fecal samples were collected daily and frozen At the end of the ... at the end of the digestive process (Sullivan and Reigh, 1995) Up to now, only few feed ingredients currently used for grass carp in Vietnam have been assessed for their digest value Thus, the ... stored at 4°C Table shows the ingredients used in the reference and three test diets The digestibility experiment was carried out with three replicates per treatment in a thermoregulated water system...

Ngày tải lên: 25/02/2014, 10:20

... However, further research is necessary to consider the implications of the cause and severity of the disability, the influence of the competitive level of sport, the mechanisms that explain the relationship ... are “acceptable” for them and how they should control their bodies through diet and exercise to enhance their “feminine” qualities as they become women Within the context of the gendered experience ... the effect of neutralizing their femininity (and) creating the perception they are genderfree” [81; p 166] Nosek [81] argues that using a wellness model that emphasizes the role of harmony, the...

Ngày tải lên: 05/03/2014, 13:20

... when the homeowner was able to obtain refinancing The rescuer recorded the quit claim deed and then sold the property Whereas in these instances, the borrower was the victim of the fraud, another ... told that if they signed a quit claim deed for the benefit of the rescuer, the mortgage would be paid and the homeowner could continue living in the house with the promise that the property would ... names to an account, then drops them shortly after the bank responds to a VOD In these cases, the account holder may have added the loan applicant’s name to the account to boost the latter’s (apparent)...

Ngày tải lên: 06/03/2014, 19:20

... constructed using the Build Model module from Discovery Studio (Accelrys, San Diego, CA, USA) The model with the best loop conformations was then selected using the Profiles-3D verification method, and the ... phenol-lyase the basis of energy minimization in the DS CHARM module employing the steepest descent method followed by the conjugate gradient method During the minimization process, the protein ... further away from the interface, as the activityrelated mutations near the interface were seemingly deleted during the reassembly–screening steps, thereby allowing the directed co-evolution of the...

Ngày tải lên: 07/03/2014, 00:20

... expressed Only the structures of GluRS from thermophilic bacteria have been solved The Thermus thermophilus enzyme (Tt-GluRS) is the structural model for D-GluRS [5,1214], and the Thermosynechococcus ... reaction at 37 C as described above, except that the concentration of one of the substrates was varied and the levels of the others xed In these assays the amount of enzyme was chosen in order to ... Rv3012c) predict the presence of the three subunits of the GlutRNAGln amidotransferase These observations suggest that the Mt-GluRS is of the nondiscriminating type Finally, genes encoding the putative...

Ngày tải lên: 07/03/2014, 03:20

... 365 480 nm The GSH content of the plasma was derived from the standard curve and the regression equation The average recovery test was made using the standard addition method, and the RSD was ... solution Thus the optimal pH of the system was in the range 4.009.00 The results are shown in Fig As can be seen, the uorescence intensity was relatively high and remained almost constant over the pH ... range 5.006.50 Therefore, a pH of 6.00 was xed using NaCl Pi buffer The effect of the buffer is lost if too small a quantity is used Whereas if the amount of buffer is excessive, the ionic strength...

Ngày tải lên: 07/03/2014, 05:20

... [17,44,45] In all of these cases, the activity was very weak and the activated state was not stable, therefore limiting the enzymatic analysis Therefore, the enzymatic properties of the phenoloxidase ... were analyzed by tting the catalytic efciency (kcat KM) in the linear part of the curve (Table 1) The lines connecting the data are shown to facilitate the identication of the different substrate ... min, and then the reaction was started by the addition of substrate The reaction was followed for by measuring the absorption at 475 nm The critical micellar concentration of SDS was varied using...

Ngày tải lên: 07/03/2014, 05:20

... adhere to the agar medium in agreement with low cellular PKA activity On the other hand, S1278b wild-type control cells showed the normal, strong invasive growth In fact, the extent of the reduction ... was constructed to contain SUT2 as the only open reading frame present in the plasmid in order to confirm the role of SUT2 as a high copy suppressor of the synthetic phenotype Ó FEBS 2004 1286 ... were then used to obtain the corresponding Dras2 (AR3) and Dsut2 (AR4) reporter strains by standard genetic methods High copy suppressor screen The Yep351-based yeast genomic library used for the...

Ngày tải lên: 07/03/2014, 15:20