words and their stories clothing expressions part one
Words and Their Stories: Number One potx
... Words and Their Stories: Numbers Part TwoNow, the VOA Special English program WORDS AND THEIR STORIES. Last week, I told about the number one. Today, I will tell about expressions ... They gave praise and approval for a job well done.Some of my friends were confused and did not understand their schoolwork. They were at sixes and sevens. In fact, they did not care if they ... can kill two birds with one stone. That is, we can complete two goals with only one effort or action. But we must remember that two wrongs don’t make a right. If someone does something bad...
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NTC's pocket dictionary of words and phrases part 75
... someone’severy word→ have one s words stick in one sthroat→ keep one sword→ not utter a word→ one sword→ one s words stick in one sthroat→ put in a good word (for someone)→ ... someone)→ put words into someone’smouth→ take the words (right) out of one smouth→ true to one sword→ twist someone’s words (around)theword 1. n. an order; a com-mand. (No plural. ... forcesomeone or something loose,down, away, etc., with force. 2.iv.to participate in a sport in whichones wrestles one s opponentdown as in Q.wringer ["rIN #] n. an old-fash-ioned...
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Báo cáo khoa học: "Extracting Opinion Expressions and Their Polarities – Exploration of Pipelines and Joint Models" pot
... segmenter and polarity classifier are based on pairs of opinions: ba-sic features extracted from each expression such aspolarities and words, and relational features describ-ing their interaction. ... ReferencesStephan Bloehdorn and Alessandro Moschitti. 2007a.Combined syntactic and semantic kernels for text clas-sification. In Proceedings of ECIR 2007, Rome, Italy.Stephan Bloehdorn and Alessandro Moschitti. ... expressionsegmentation and polarity labeling and comparethem to the joint opinion extractor and polarity clas-sifier by Choi and Cardie (2010). Secondly, we ex-tend the global structure approach and add featuresreflecting...
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Báo cáo khoa học: "Self-Organizing Markov Models and Their Application to Part-of-Speech Tagging" potx
... ti and is estimated by using Good-Turingestimation (Gale and Samson, 1995). If the word, wiis an unknown word, kiis set to 0 and the first termis ignored. eirepresents suffix of wi and ... (C),prepositions (P) and verbs (V). The dollar sign ($)represents sentence initialization. On the left handside is the graph representation of the Markov model and on the right hand side is the ... techniques and with handling of unknown words. While global optimization is the strong point ofHMMs, developers often complain that it is difficultto make HMMs incorporate various features and toimprove...
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small gtpases and their regulators, part a
... removal of the AAX residues, and finally, carboxyl i H. R. Bourne, D. A. Sanders, and F. McCormick, Nature (London) 348, 125 (1990). 2 H. R. Bourne, D. A. Sanders, and F. McCormick, Nature ... for Immunol- ogy and Respiratory Medicine, Denver, Colorado 80206, and Department of Phar- 64 EXPRESSION, PURIFICATION, AND MODIFICATION [7] prolonged incubations alone do not yield an ... 118 and 220 mg, respectively. The GDP- binding stoichiometries and GTPase activities for soluble Ras and refolded Ras isolated from the same cells were measured. Refolded H-Ras-bound GDP and...
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small gtpases and their regulators, part b
... GDP-bound form and an active GTP-bound form, and their interconversion occurs through a cycle of guanine nucleotide exchange and GTP hydrolysis. The intrinsic nucleotide exchange and GTPase activities ... M. Sizeland, and R. A. Weinberg, Nature 363, 45 (1993). 16 y. Zheng, R. A. Cerione, and A. Bender, J. BioL Chem. 269, 2369 (1994). 17 T. Miki, C. L. Smith, J. E. Long, A. Eva, and T. Fleming, ... transduction in their own right. 6-s The i S. E. Neal, J. F. Eccleston, and M. R. Webb, J. Biol. Chem. 263, 19718 (1989). A. Hall and A. J. Self, J. BioL Chem. 261, 10963 (1986). 3 L. Buday and J....
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small gtpases and their regulators, part c
... separate components: a catalytic component (originally named component B) and an accessory subunit (also called component A). 6-1° The catalytic component is a heterodimer composed of a and/ 3 subunits. ... transformants and plasmid DNA is extracted. Coexpression of Bet2p and Bet4p in E. coli and Preparation of Cell Lysates In order to coexpress Bet2p and Bet4p in E. coli, plasmids pSFN171 and pSFN173 ... U/ml penicillin, and 100/~g/ml streptomycin and are incubated for 3 days at 27 °. The cells are harvested and pelleted by centrifugation at 1000g for 10 min at 4 °. After one wash with 50 ml...
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small gtpases and their regulators, part d
... Triton X-100 and 1/~M GDP and twice in buffer GSH containing 1 ~M GDP. The GST-GTPases are liberated by two sequential elutions in 1 ml of buffer GSH containing 10 mM reduced glutathione and incubation ... KENT L. ROSSMAN (3), Department of Bio- chemistry and Biophysics, University of North Carolina, Chapel Hill, North Caro- lina 27599-7260 LURAYNNE C. SANDERS (28), Department of Immunology, ... White, and D. Broek, Science 279, 558 (1998). ~' S. Katzav, D. Martin-Zanca, and M. Barbacid, EMBO J. 8, 2283 (1989). 7 M. J. Hart, A. Eva, T. Evans, S. A. Aaronson, and R. A. Cerione,...
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small gtpases and their regulators, part e
... (DeepVent, New England Biolabs, Frankfurt/Main, Germany) and high concentration of DNA template are used. Each gene (or fragment) is amplified in triplicate, and PCR products are cloned and tested ... cDNAs of p130 and p150 have been cloned from a human brain cDNA library, and the encoded proteins show no homology to any known protein. TM By Northern blot analysis, both p130 and p150 are ... made by mixing Vasoline, lanolin, and paraf- fin in a 1:1:1 ratio and liquefies when heated to 40-50 °. On cooling, Valap solidifies and creates a hardened, air- and watertight seal around the...
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small gtpases and their regulators, part f
... density values and standard error are shown. The synthetic peptide ligands of the Src and Abl SH3 domains are SGSGVLKRPLPIPPVTR and SGSGSRPPRWSPPPVPLPTSLDSR, respectively. The peptide ligands of ... PROTEIN EXPRESSION AND PROTEIN-PROTEIN INTERACTIONS [ 11 and human epithelial cell types including DLD-1 and HCT116 colon cancer cells and MCF-10A breast cells. Retroviral Vectors and Viral Packaging ... digestion with BgllI and ApaI are added to each tube and incubated at 14 ° for 12 hr. The complementary strands anneal to form linear double-stranded DNA (dsDNA) and are subsequently ligated...
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small gtpases and their regulators, part g
... either Rat-1 cells (a and b) or NIH 3T3 cells (c) and tested using myelin basic protein (MBP) as a substrate. The upper band is the PAK autophosphorylation band and the lower band is MBP. The radioactivity ... blot of 25 ~±g of lysate from PFSK (lanes I and 4), newborn mouse brains (lanes 2 and 5), and A673 (lanes 3 and 6), probed as described in (C) and (D) with either ShcA or ShcC antibodies. methionine ... 23298-0058 GEORGE F. VANDE WOUDE (28), Van Andel Researeh hls'titute, Grand Rapids, Michigan 49503 MIRANDA VAN TRIEST (30), Del?atTnlent ~}[ Physiological Chemistry and Centre for Bionledical...
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Báo cáo hóa học: " One-Pot Synthesis of Biocompatible CdSe/CdS Quantum Dots and Their Applications as Fluorescent Biological Labels" doc
... Program for Graduates (2008022), and the foundation of 2008DFR50250.Author details1State Key Lab of Silicon Materials and Department of Materials Science and Engineering, Zhejiang University, ... due to their suitable and tunable band gap throughout the visible spectrum[3]. The high-temperature chemica l reaction was a well-known approach for the synthesis of highly crystalline and monodisperse ... cooled to room t empera-ture, and 19 mg of Se powder was added. Finally, themixture was heated to 240°C and kept for a certainperiod of time such as 1, 5, 60, and 120 min.Synthesis of CdSe/CdS...
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