wnt catenin signaling pathway target genes

... dephosphorylated b -catenin without changes in total b -catenin, suggesting that a-defensins activate the b -catenin signaling pathway We then studied the role of the b -catenin signaling pathway in a-defensininduced ... kinase and PI3K ⁄ Akt The Wnt ⁄ b -catenin signaling pathway is required for proper lung mesenchymal growth and vascular development [12,28] Up-regulation of the Wnt ⁄ b -catenin pathway was observed ... fibroblasts Cell proliferation mediated by the b -catenin signaling pathway is attributed to the direct target genes of b -catenin- TCF ⁄ LEF-1, such as the genes for cyclin D, fibroblast growth factor,...

Ngày tải lên: 18/02/2014, 06:20

... the PCP pathway [7,8], this observation suggests that DsNLSm can respond to Frizzled signaling independent of ␤ -catenin (a) Dsh Volume 4, Article Figure Activation of the Wnt/ ␤ -catenin pathway ... ␤ -catenin pathway Volume 4, Article Itoh et al 3.5 Not only was the function of DsNLSm in the ␤ -catenin pathway impaired, but we found that this construct behaved as a dominant inhibitor of Wnt ... critical components of the Wnt/ ␤ -catenin pathway, arguing that defective nuclear translocation of DsNLSm is likely to be responsible for its inability to activate ␤ -catenin signaling Suppression of...

Ngày tải lên: 06/08/2014, 18:21

... et al 2004) 1.3 Wnt/ β -catenin pathway 1.3.1 Association of Wnt/ β -catenin pathway with chondrocyte dedifferentiation Dedifferentiation has been linked to Wnt/ β -catenin signaling pathway (Hwang, ... dedifferentiation 15  1.3  Wnt/ β -catenin pathway 17  1.3.1  Association of Wnt/ β -catenin pathway with chondrocyte dedifferentiation 17  iii 1.3.2  Curcumin as a Wnt/ β -catenin antagonist ... of β -catenin transcriptional activity and rescued the suppression of collagen II induced by Wnt/ β -catenin activation (Hwang, Ryu et al 2004) β -catenin is an effector in the Wnt signaling pathway...

Ngày tải lên: 16/10/2015, 15:38

... known genes and 19 ESTs (supplementary tables S2, S3) Nineteen of 1601 Wnt target genes and the regulation of their promoters these candidate genes have already been described as target genes in Wnt- activated ... oncogenic Wnt- 1 [13–15] The resulting C57MG ⁄ Wnt- 1 cells have been used in many studies to characterize the Wnt pathway and to identify novel Wnt target genes [16–20] The culture of C57MG ⁄ Wnt- 1 ... insights into the regulation of promoters of potential target genes of the Wnt pathway, which we identified by microarray screening Results Wnt target genes and the regulation of their promoters A B...

Ngày tải lên: 30/03/2014, 16:20

... behaviors of this pathway and how it interacts with other pathways [2,10-13] The wnt pathway is one of the computationally best-studied signaling pathways [14-16] The kinetics of β -catenin, axin, ... http://www.tbiomed.com/content/6/1/13 -catenin* + -catenin* -catenin* /APC*/Axin*/GSK3 -catenin/ APC*/Axin*/GSK3 wnt DSHI DSHA APC/Axin/GSK3 18 GSK3 APC*/Axin*/GSK3 GSK3 APC/Axin Axin APC/ -catenin -catenin/ TCF -cateni ... Lee-Heinrich model of the wnt pathway itself, this model has been used and extended to examine the effect of Apc mutations on the wnt signaling pathway [10], cross-talk with the ERK pathway [11], and...

Ngày tải lên: 13/08/2014, 16:21

... activated ERK (Fig 5C) Analysis of GBP signaling pathways It is now apparent that the GBP signaling pathways diverge from a certain component into at least two pathways: one towards ERK activation, ... the GBP signaling pathway requires MEK activity (Fig 5B) Furthermore, to test whether the GBP-induced expression of TH and DDC genes requires ERK activation, expression of both enzyme genes was ... transcriptional enhancement of TH and DDC genes (Fig 7) Although the GBP signaling pathways were characterized by analyzing MaBr4 cells, it is reasonable to assume that these pathways are employed in armyworm...

Ngày tải lên: 30/03/2014, 04:20

... that these genes clustered in several signaling pathways, including the insulin, MAPK, Wnt, TGF-beta, Notch and mTOR signaling pathways, and apoptosis Five of the differentially expressed genes involved ... LMP1 regulates mTOR signaling in NPC tumorigenesis Deregulation of the mTOR signaling pathway is reported in many malignancies, and some of the signaling molecules in this pathway are predictors ... activate the PI3K/AKT/ mTOR signaling pathway in B lymphocytes [21], and the mTOR signaling pathway has been identified as a downstream component of the PI3K/AKT pathway in the LMP2A-transfected...

Ngày tải lên: 18/06/2014, 16:20

... TGF-β signaling pathways (Bollmann 2008) Apart from the TGF-β signaling pathway, hTERT was also found to be part of the Wnt/ β -catenin signaling pathway Initial documentation of a link between catenin ... 3.2 3.2.1 3.2.2 3.3 3.3.1 3.3.2 3.3.3 Telomerase related screen Wnt signaling pathway Canonical Wnt pathway Non-canonical Wnt pathway Material and Methods Material Cell line Inhibitor library ... pathway either by Wnt ligand (Wnt- 3a) or LiCl (activates VII Wnt signaling by inhibiting GSK-3β) treatment as well as overexpression of a constitutively active form of β -catenin (Δ-N β -catenin) up...

Ngày tải lên: 02/10/2015, 17:14

... known NF-jB targets by collecting known NF-jB targets mentioned in an NF-jB review paper [25] matching ensembl entries, resulting in 91 genes Joining the 91 target genes with the genes in the ... all NF-jB target genes mentioned in an NF-jB review paper [25] These genes were matched to human ensembl gene entries, resulting in 81 NF-jB seed genes Joining those genes with available genes in ... sizes than the 81 genes used in the NF-jB seed The list of predicted NF-jB targets and experimental verification We assembled a list of predicted NF-jB target genes by selecting all genes that showed...

Ngày tải lên: 18/02/2014, 18:20

... of a substantially larger subset of genes Identification and characterization of a subset of ISGF3-independent STAT2-dependent target genes To identify those genes whose expression was exclusively ... ISGF3-independent STAT2-regulated signaling pathways that might influence IFN-inducible outcomes, by generating pathway networks downstream of IFNAR that highlighted genes cited in this study (Fig ... the most differentially regulated genes Finally, these genes were 1576 validated by real-time PCR and placed into the context of IFN-related biological pathways Target gene binding by STAT complexes...

Ngày tải lên: 19/02/2014, 07:20

... Ó FEBS 2002 5862 G Pintus et al (Eur J Biochem 269) through tumor-induced angiogenesis [12] Therefore, targeting endothelial cells with antiangiogenic drugs may represent a useful implementation ... selectively inhibit the PMA-dependent signaling leading to the phosphorylation of ERK1/2 isoenzymes (Fig 2B) To verify the involvement of a PKC-dependent pathway in both serum- and PMA-generated ... line, has been recently proposed as a suitable model for the investigation of the signaling mechanisms governing angiogenesis under both normal and pathological conditions [6,15] A recent investigation...

Ngày tải lên: 21/02/2014, 01:21

... Role of Bud32p in a new signaling pathway in yeast C Peggion et al polypeptide in some archaeans, and their human homologs are ... revealed with specific antibodies for Bud32p or Grx4p (right panel) Role of Bud32p in a new signaling pathway in yeast ity of native Bud32p This behavior could be related to subtle differences ... 5919–5933 ª 2008 The Authors Journal compilation ª 2008 FEBS 5921 Role of Bud32p in a new signaling pathway in yeast C Peggion et al A C B D Fig Bud32p coimmunoprecipitates with and phosphorylates...

Ngày tải lên: 07/03/2014, 04:20

... variety of components of the NF-jB signaling pathway in single cells [11,12] These results agreed with in silico simulations of the downstream region of the pathway that was modeled, as well as ... nucleus where it induces the transcription of a large number of target genes encoding regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In ... of a known inhibitor of IKK2, 4-amino-2,3Â-bithiophene-5-carbox- In vitro analysis of NF-jB signaling pathway amide (SC-514) [38] We initially performed an in vitro study which conrmed the competitive...

Ngày tải lên: 16/03/2014, 11:20

... investigated the signaling pathway of b-ARinduced STAT3 activation It is well known that b-AR stimulation can activate the mitogen-activated protein kinase (MAPK) signaling pathway [15,16] On ... gp130 signaling pathway in STAT3 activation Consistent with our hypothesis, ERK1 ⁄ is required for the production of many cytokines in a wide variety of cells [21–23] The gp130 ⁄ STAT3 signaling pathway ... Cruz, CA, USA), and anti-phospho-tyr705-STAT3 (Cell Signaling Technology, Beverly, MA, USA) Luciferase reporter assay Fig gp130 ⁄ JAK2 signaling pathway was involved in b-ARinduced ANF expression...

Ngày tải lên: 23/03/2014, 07:20

... and then, downstream of the signaling pathways, branches into two pathways: the mitochondria-independent path way and the mitochondria-mediated pathway The former pathway involves the proteolytic ... development of PPC enables us to pinpoint the signaling pathways The steps in the pathway have mainly been disclosed in trials using experimental animals In IPC, key signaling elements are mitochondrial ... discuss the mitochondria-mediated pathway in intrinsic and extrinsic apoptosis in more detail As noted above, mitochondria are deeply involved in the apoptotic signaling pathways via the release of...

Ngày tải lên: 23/03/2014, 07:20

... mRNA levels of both SREBPs and their target genes became significantly elevated at and 12 h (Fig 4) Based on the fact that the increase in the mRNA of these genes was nearly simultaneously observed ... the expression of the genes involved in cholesterol homeostasis The results clearly demonstrate that glutamine stimulates SREBP processing and the gene expression of SREBP targets The same concentrations ... activities and stimulates the gene expression of SREBP targets Results and Discussion Glutamine stimulates the promoter activities of SREBP targets More than 50 years ago, Eagle et al [8] reported...

Ngày tải lên: 28/03/2014, 22:21

... regulator of the Wnt signaling pathway downstream of Wnt and upstream of Dvl, axin and b -catenin CKIe and Dvl physically interact in vivo, and CKIe phosphorylates Dvl in response to Wnt signaling [9–12] ... modified by wingless signaling in Drosophila Genes Dev 9, 1087– 1097 Kuhl M, Sheldahl LC, Park M, Miller JR & Moon RT (2000) The Wnt ⁄ Ca2+ pathway: a new vertebrate Wnt signaling pathway takes shape ... The results provide molecular insights into the mechanism by which Wnt signaling to CKIe regulates the b -catenin signaling pathway Results and Discussion CKIe phosphorylates mouse Dvl-1 (mDvl-1)...

Ngày tải lên: 30/03/2014, 10:20

... on more than one signaling pathway for their growth and survival Therefore, investigators pursue different strategies to inhibit multiple signaling pathways by developing multitargeted agents ... one such multitarget agent that targets both the antiapoptotic and cell cycle progression pathway in NPC cells by blocking antiapoptotic Bcl-2 proteins and the c-Myc oncogenic pathway These findings ... not affect cell cycle progression or the p53 or p21/WAF signaling pathway in A549 human alveolar lung cancer cells [10] Different oncogenic pathways are activated in different types of cancer, and...

Ngày tải lên: 18/06/2014, 15:20

... and expression of protein Cyclin A via the TK signaling pathway Taking together, PAP promotes chondrocyte proliferation by activating TK signaling pathway Acknowledgements This study was supported ... promoting effects of PAP on chondrocyte proliferation and implicates the involvement of TK -signaling pathway The proliferation of chondrocytes was evaluated directly with MTT assay, and indirectly ... suggest that PAP promotes chondrocyte proliferation by TK-mediated signaling Future experiment will be focused on the downstream signal pathway of TK and its regulatory mechanism(s) Author details...

Ngày tải lên: 20/06/2014, 00:20

... Cur61414 Membrane Hip1 Gas1 Ptc Smo Cos Forskolin CK1 PKA GSK3 Rab23 Dyrk1 Nucleus Gli Target genes Target genes (b) Hh signaling Gli-rep of Hh, Gli3 is processed into a smaller, nuclear transcriptional ... harbor an active pathway - and Ptc+/- mice can develop medulloblastomas ([15-19]; reviewed in [13]) Fu SuFu http://jbiol.com/content/1/2/9 Gli-act Target genes Figure The Hh -signaling pathway (a) ... diagram of the Hh -signaling pathway, showing the site of action of the agonists (green) and antagonists (red) discussed in the text, as well as many additional factors that affect the pathway Abbreviations:...

Ngày tải lên: 06/08/2014, 18:20