whole cell organism sensors and reporter genes

... fish and meat wastes) Indeed, they can solubilize proteins present in waste streams and generate products with added nutritional value (9) Similarly, cellulases may hydrolyze lignocellulose and cellulose ... studies performed on whole cells and extracts of D tiedjei cells Sulfate, sulfite, and thiosulfate showed separate mechanisms of inhibition, depending on the growing conditions and the presence or ... 2,3,5,6-tetrachlorophenol, and 2,3,4,5,6-pentachlorophenol (158) The ToMO genes were cloned in E coli and the expressed enzyme degraded TCE, 1,1-DCE, and chloroform very efficiently (i.e., very low K m values and high...

Ngày tải lên: 11/08/2014, 15:20

... (filer paper), BWX and PWSD The enzyme kinetic studies for cellulase and xylanase (Km and Vmax) were performed with 1-10 mg/ml of CMC and BWX, respectively [35] The crude cellulase and xylanase were ... that cellulases exhibit maximum activity at both acidic and alkaline pH [52, 53] Figure Effect of pH (at 600C) on the cellulase and xylanase activity of the carboxymethyl celluloseenriched and ... showed both cellulase and xylanase activity A 35 kDa protein was reported for a T aurentiacus cellulase [62], a B subtilis B230 xylanase [63] and a Postia placenta multienzyme cellulase and xylanase...

Ngày tải lên: 05/09/2013, 16:11

... malignant cells [4] However, it is still unclear whether the unusual intracellular trafficking of the endocytosed RNases in transformed cells [6] or the altered cell surface carbohydrate and lipid ... [18], and dimerization by 332 domain swapping has been suggested to cause cytotoxicity by improved cellular uptake [19] Tandemization considerably enhances endocytotic internalization into the cells ... substrate] and cytotoxic (IC50 values between 12.9 lm and 40.0 lm with mammalian K-562 cells) All three variants showed thermodynamic stabilities similar to that of monomeric RNase A, and were...

Ngày tải lên: 22/03/2014, 16:21

... r) D0 with Ki ∈ X and G = K1 , , Kr ; X = (G : G = H1 × · · · × Hr with each Hi ∈ X); EΦ X = (G : there exists N G with N ≤ Φ(G) and G/N ∈ X) The operations Sn and Q, and N0 and R0 are dual ... the first and third properties leading to the theory of saturated formations and Schunck classes and the associated projectors and the second property to the theory of Fitting classes and injectors ... π-special and π -special irreducible KG-modules, then U ⊗W is irreducible Moreover, if U and W are respectively π-special and π -special irreducible KG-modules, and U ⊗ W ∼ = U ⊗ W , then U ∼ U and...

Ngày tải lên: 12/05/2014, 01:59

... sealed, the autoclave was put in an oven and heated at 130°C for h After reaction, the foil was taken out of the autoclave and washed with water for several times and then dried in air X-ray diffraction ... distribution, and reproduction in any medium, provided the original author(s) and source are credited References Fig a Typical Ids–Vds curves obtained at different gate voltages and b Ids–Vg curve ... the [0001] orientations These micro/nanotubes show strong nearband-edge emission at 387 nm and weak defects-related emissions at 422 and 485 nm, respectively Contact angle result indicates they...

Ngày tải lên: 22/06/2014, 00:20

... and Construction Influence on the Tire Safety 265 Pavel Koštial, Jan Krmela, Karel Frydrýšek and Ivan Ružiak Chapter 14 Friction and Wear of Polymer and Composites Dewan Muhammad Nuruzzaman and ... functional and structural nanocomposites, numerical and theoretical modelling of various damages in textile and long fiber reinforced composites, design, processing and manufacturing technologies and ... breaker construction and rubber blend Chapter 14 Friction and Wear of Polymer and Composites In this chapter, friction coefficient and wear rate of different types of polymer and composite materials...

Ngày tải lên: 28/06/2014, 10:20

... John Smith and Claire Simons Cardiff Univeristy Cardiff, UK Carbonic Anhydrase: Its Inhibitors and Activators Edited by Claudiu T Supuran, Andrea Scozzafava and Janet Conway Enzymes and Their ... Example Claudiu T Supuran, Andrea Scozzafava, and Angela Casini 2.7 Proteases Example André J Niestroj and Hans-Ulrich Demuth Chapter Kinetics Irene Lee and Anthony J Berdis Chapter Enzyme Inhibitors ... Cataloging-in-Publication Data Enzymes and their inhibition: drug development / edited by H John Smith and Claire Simons p.; cm — (Enzyme inhibitor) Includes bibliographical references and index ISBN 0-415-33402-0...

Ngày tải lên: 02/07/2014, 11:20

... trafficking, cell growth and cytoskeletal rearrangements (Carman and Henry, 1999; Dowhan, 1997; Odorizzi et al., 2000) Considering their multi functional roles in the cells, a deeper understanding ... falcon tubes The cells were grown to an O.D of 0.8-0.9 and harvested at 3500 rpm for The cells were washed in 1ml of Milli-Q water and transferred to a 2ml eppendorf tube The cells were then spun ... Lithium acetate and transferred to a 1.5 mL microfuge tube The cells were pelleted down at 8000 rpm for 15 sec and supernatant discarded The cells were resuspended in 400 µL of 100 mM LiAc and vortexed...

Ngày tải lên: 04/10/2015, 16:03

... kox and kred are kcat values for alcohol oxidation and ketose reduction; KiNADH and KiNAD are dissociation constants for NADH and NAD+; and KmRO and KmROH are Michaelis constants for ketose and ... reported random binding of NAD+ and Man-ol1P [29] It is reasonable to assume that random binding of reactants by AfM1PDH and AfM2DH occurs in rapid equilibrium, and the absence of curvature in ... binding Discussion Kinetic mechanism of Af M1PDH and Af M2DH The theory developed by Cook and Cleland is used to deduce the kinetic mechanism of AfM1PDH and AfM2DH from KIE data in Table [28] The pattern...

Ngày tải lên: 14/03/2014, 23:20

... for sandpaper The chief types of abrasive sand include sandblast sand, glass-grinding sand, and stone-cutting sand Sand for stone sawing and for marble and glass grinding is usually ungraded, with ... a solvent for nitrocellulose and cellulose acetate, for gums and resins, in lacquers and thinners, and in ink, paint, and varnish removers Because of its low freezing point and miscibility with ... have tough, uniform grains Chats are sand tailings from the Missouri lead ores, used for sawing stone Banding sand is used for the band grinding of tool handles and for the grinding of plate glass,...

Ngày tải lên: 13/02/2014, 09:20

... extended towards Cys183 in ChiC (and in the plant enzymes) Compared to the barley enzyme, ChiC and ChiG lack three loops (A, B and C) and a C-terminal extension (Figs and 4B) In addition, one other ... [39] and scaled and merged with scala in CCP4 [40,41] The crystals belong to space group P21 with cell ˚ ˚ ˚ dimensions a ¼ 48.67 A, b ¼ 74.38 A, c ¼ 64.18 A and ˚ resolution b ¼ 108.6°, and diffracted ... experiments and helpful discussions, and May Bente Brurberg and Svein Horn for helpful discussions The authors acknowledge the beamline staff at ID14-EH3 for technical assistance, and the ESRF and the...

Ngày tải lên: 07/03/2014, 11:20

... expansins loosened cellulosic paper [14] and that proteinases did not mediate plant cell wall extension in vitro [19,20] EXPERIMENTAL PROCEDURES Site-directed mutagenesis and subcloning Phl p ... identity between Hol l and CP2 was  22%, between Phl p and CP1 as well as CP3 19%, and between Phl p and CatB  15% Moreover, the putative active-site amino acid Cys72 of Phl p and Hol l is very ... cysteine residues and other essential amino acids are numbered Areas of high similarity exist around Cys69 and Cys72 and around Trp186, Trp193 and Trp197 The positions of Cys41, 57, 69 and 72 are especially...

Ngày tải lên: 08/03/2014, 22:20

... seawater handling within offshore, desalination, and coastal located process industries Some crevice corrosion has still been reported and for more severe situations, i.e severe crevice geometries and ... welded with preheating and postweld annealing Today’s ferritic steels of type S44400 and S44635 have considerably better weldability due to low carbon and nitrogen contents and stabilization with ... petrochemical sector, seawater-bearing systems and the offshore industry COMPOSITION AND MECHANICAL PROPERTIES The mechanical properties, corrosion resistance and weldability of a steel are largely determined...

Ngày tải lên: 14/03/2014, 12:21

... for sandpaper The chief types of abrasive sand include sandblast sand, glass-grinding sand, and stone-cutting sand Sand for stone sawing and for marble and glass grinding is usually ungraded, with ... a solvent for nitrocellulose and cellulose acetate, for gums and resins, in lacquers and thinners, and in ink, paint, and varnish removers Because of its low freezing point and miscibility with ... have tough, uniform grains Chats are sand tailings from the Missouri lead ores, used for sawing stone Banding sand is used for the band grinding of tool handles and for the grinding of plate glass,...

Ngày tải lên: 14/03/2014, 16:20

... clearly indicates that these nanowires are of uniform size and smooth surface, and the average diameter and length of these nanowires are 80 nm and ␮m, respectively The energy dispersive X-ray spectroscopy ... single crystalline and grows along [12-1] direction, which is different from common [1 1] and [1 0] growth direction [26,27] No obvious defects and dislocations are observed, and the interplanar ... decomposed into Sn and SnO vapor Sn and SnO vapor subsequently are transported to low temperature zone and form liquid droplets The liquid droplets gradually absorb oxygen and are further oxidized...

Ngày tải lên: 19/03/2014, 16:48

... mortadella and galantines) from different commercial brands were purchased in retail shops in Spain The 24 samples were 691 bought three times (January, May and October) maintaining brands and meat ... behaviour between profiles and with similar (p > 0.05) values of BS and hardness to textural profile 1, and similar values (p > 0.05) of cohesiveness and springiness to profile 3, and intermediate values ... mean of each sample and the standard deviation (SD) of the mean Results and discussion 3.1 Physico-chemical analysis Dry matter, fat content (% dry matter), water activity (aw) and pH values of...

Ngày tải lên: 05/05/2014, 08:43

... multiple stands from chair, standing balance, step-up and ambulation Approximately 10 minutes [29] 8.6 minutes (SD = 3.6 minutes) [41] A bed, chair, stop watch, standardised step and gait aid ... person hands-on, person standby, independent with aid, independent BALANCE: impaired static sitting, stable static sitting, stable dynamic sitting, stable static standing, stable dynamic standing, ... scores (and standard deviations) and between groups comparison data were extracted as evidence of 'known groups' validity Data that indicated a relationship between mobility instrument scores and...

Ngày tải lên: 18/06/2014, 22:20

... and average surface roughness (Sa) of all pipettes have been measured Figures 3, 4, 5, and show correlations between pulling parameters and Dt and Sa As it can be seen from Figures 3, 4, 5, and ... leakage between cell membrane and glass surface Cell membrane is sucked from to 100 μm into a pipette in patch clamping Optical and electron microscope images of patches show that membrane and pipette ... angles -5° (a), 0° (b), and 5° (c), DEM created using MeX (d) For surface texture parameters of this pipette please see Tables and The bar represents 10 μm Cell culture and patch-clamping experiments...

Ngày tải lên: 21/06/2014, 03:20

... 0.05 1.116 Spherical and square [19] Not available 0.05 1.036 - 130-200 75-100 0.05 0.1 1.085 1.238 Spherical and square Spherical and square 50-100 0.1 1.238 Spherical and square 100-300 0.1 ... volume fraction, and temperature [66] Xue and Xu derived a model which consists of the thermal conductivity of the solid and liquid, their relative volume fraction, the particle size and interfacial ... the temperature and the volume fraction dependence of the thermal conductivity of nanofluids for both non-flowing and flowing fluids [57] Murugesan and Sivan developed lower and upper limits...

Ngày tải lên: 21/06/2014, 04:20

... 500 and 700 cm-1, which is different from Fe3O4 [40, 41] The IR analysis combined with TEM images and XRD pattern can confirm the synthesis of a-Fe2O3–Au and Fe3O4–Au hybrid nanorods Fe3O4–Au and ... resonance band at around 520 nm [44] The exact absorption varies with nanoparticles morphology and particle surface coating Herein, compared to pure Fe3O4 and aFe2O3 nanorods, both Fe3O4–Au and a-Fe2O3–Au ... process (a) and with two-layer (PAH/PSS) assembly (b) from iron oxide nanorods and Au nanoparticles, respectively Figure shows the room-temperature magnetization curves of Fe3O4–Au and a-Fe2O3–Au...

Ngày tải lên: 21/06/2014, 07:20