... carnitine: 1 mm (for standard comparison withother published data), and a concentration equal tothe Km for carnitine of each enzyme (for comparisonbetween mutants). In this article, we show ... sequence as being responsiblefor the pecu-liar kinetic characteristics of this enzyme, acting as anegative determinant for malonyl-CoA sensitivity.Asp17 may account, at least in part, for the highdegree ... nat-urally occurring variation, Glu17, isresponsiblefor both the low malonyl-CoA sensitivity and high carnitine affinity characteristics of the pig enzyme.This is the first demonstration that a single...
... active conformation, Val371 forms contactswith neighboring residues that are important for stabi-lizing the active state (e.g. Asp189, which is part of theS1 pocket responsiblefor the binding ... sequence identity of 30–45%), thisposition is occupied solely by a valine. This conservedamino acid is replaced by isoleucine in the Val371IleFXI mutant. Interestingly, an isoleucine residue natu-rally ... thesubstrate) [26]. Consequently, substitution of Val371 toisoleucine might prevent the full development of theactive conformation. This hypothesis is further con-firmed by the results of FIX proteolytic...
... factor that favors lysogeny and is responsiblefor primary control of the lambda develop-mental decision for lysis or lysogeny [4,7–9]. In theabsence of CIII, CII is rapidly degraded by the ATP-dependent ... However, this proteolysis is muchslower in vitro, because of a lack of chaperone machin-ery. The in vitro proteolysis requires ATP [14]. Weexamined the proteolysis of purified r32-C-his (1.2 ... CIII-mediated inhibition of proteolysis of r32by HflB is unknown.We tried to address this issue by studyingthe effects of CIII and CIIIC on the proteolysis ofKeywordsantiproteolytic activity;...
... synthesis, it might be involved in the stearicacid incorporation after the de novo synthesis of thislipid (i.e. the second hypothesis). According to thishypothesis, previously raised for mammals ... fromthe European S. cerevisiae Archive for Functional Analysis(EUROSCARF) library. BY4742 (MATa; his3D1; leu2D0;lys2D0; ura3D0) is a wild-type strain and psi1D (MATa;his3D1; leu2D0; lys2D0; ... strongly that PI is remodeled in microsomalmembranes before being transferred to mitochondriaor, in other words, that Psi1p is located in the micro-somal membranes. This finding is in agreement...
... prior to hydrolysis. The low-affinity state wasobserved to be retained immediately post-hydrolysis. Therefore, the dissoci-ation of phosphate and ⁄ or ADP is likely to be responsiblefor resettingof ... of ATPper se is the ‘power stroke’ for drug translocation, andthat energy obtained from the hydrolysis process is used to reset the transporter. That ATP binding is responsiblefor the shift ... thatABCG2R482Gremains in a conformation that containsa low-affinity binding site for [3H]daunomycin imme-diately post-nucleotide hydrolysis.DiscussionA precise molecular mechanism for substrate translo-cation...
... hydrolysis), corresponding to that expected for theF variant. This result is the first evidence for the heterozy-gous status (M/F) of the individual goat milk analysed.In addition to this, t ... subsequently distin-guished after a second amplification which allows discrim-ination between the alleles on the basis of the f ragment sizes(Fig. 8 , Step IIA).In the first step, a 266-bp (265 bp for ... the origin of the as1-CasM allele. This is, to our knowledge, t he first hypothesis o f a genomicrecombination event to account for genetic polymorphismat a locus encoding a milk protein.MATERIALS...
... emission at 340 nmwhen Fura-2 is bound to calcium; B, excitation and emis-sion at 380 nm when Fura-2 is free). Intracellular calcium levels can then be calculated from Eqn (1) where Kd is ... clinical model that is giving novel molecularmechanistic insight is adenocarcinoma of the oesopha-gus [2]. This cancer is one of the fastest rising cancersin the west, is taking the place ... MoravianBiotechnologies. Calcium determinationsThe fluorescent indicator Fura-2 (Molecular Probes) wasused to measure cytosolic-free calciumlevels in cells. WhenFura-2 is bound to calcium it is excited...
... receiving no additional information,except for the list of ingredients. The written informationwas provided on the front page of the home-use booklet.Preliminary data analysis revealed no significant ... presence of health-related information. Since the effectsof information were negligible, and not relevant enough for this study, the data were pooled together over theinformation conditions.Olfactory ... especially the managing director Merja Scharlin, is thanked for providing samples and for co-operation. Thisstudy was financially supported by the European Commis-sion’s Quality of Life Fifth Framework...
... healthy adults may be at increased risk for problems. What Is Avian Flu? Avian flu, also called bird flu, is a disease found in wild and farm birds. There is a concern that the bird flu will ... 3. Listen for Information Stay calm and get as many facts as you can. ã Listen for reports on the television, radio or Internet. ã Go to www.pandemicflu.gov or www.ohiopandemicflu.gov for ... prepare: 1. Make a plan for you and your family at home. Plan for how a pandemic might affect your work. 2. Prepare an emergency kit of supplies. 3. Listen for information about what to do and where...