Molecular Farming of Antibodies in Plants
... expressed in plants. Heavy-chain derivatives are shown in gray and light-chain derivatives in pink.Solidblack lines indicate continuation of the polypeptide backbone (simple lines indicate the ... in gray and light chains in pink.Solidblack lines indicate continuation of the polypeptide backbone (simple lines indicate the constant parts of the antibody, curly lines indicate the variable ... of other valuable proteins using this system, including avidin and β-glucuronidase. Initial findings for the expression of a secretory IgA in maize showed that the four chains were expressed, directed...
Ngày tải lên: 25/10/2013, 05:20
Regulating Phytonutrient Levels in Plants – Toward Modification of Plant Metabolism for Human Health
... others being delphinidin, peonidin, pelargonidin, petunidin, and malvidin. Of the flavonols, quercetin, kaempferol, myricetin, and isorhamnetin are common in fruits, quercetin being the predominant ... organisms. The methods for increasing, preventing, or redirecting flux into or within the path- way include increasing levels of a rate-limiting biosynthetic enzyme, inhibition of the activity ... third predominant flavonoid group in fruits is proanthocyanidins and their monomer units, catechins (procyanidin) or gallocate- chins (prodelphinidins). Delphinidin-derived anthocyanins are known...
Ngày tải lên: 25/10/2013, 05:20
Đề tài " Energy Sector Liberalisationand Privatisation in Thailand - Tự do hóa ngành năng lượng và Tư nhân ở Thái Lan "
... baht/share) National Energy Policy Office Energy Industry Act ã In order to introduce retail competition and create a power pool, a new energy law will be needed. ã Energy Industry Act is being drafted ... Policy Office Competition in Gas Supply Industry ã NEPOs plan for regulatory & industry structure of GSI – division of transmission and trading – licensing all operators – independent regulatory ... National Energy Policy Office Structure of PTT Gas PTT/PTT Co., Ltd./(Plc) PTT Gas Supporting Units GSP Supply & Marketing ã Supply ã Marketing ã Operation ã Marketing PTT Trans....
Ngày tải lên: 07/11/2013, 02:15
Tài liệu Phytohormones and Abiotic Stress Tolerance in Plants ppt
... Furthermore, minimal binding domain of BRI1 was determined using binding assays and recombinant BRI1 fragments (Kinoshita et al. 2005). In other plant species, it was also shown that mutations in BRI1 ... receiver domain and a long C-terminal part containing a single-repeat MYB-type DNA- binding domain (Sakai et al. 1998) called a GARP domain (Riechmann et al. 2000) and the proline- and glutamine-rich ... et al. 2002) were determined as positive regulators in ABA signaling. Gene encoding ABA-induced protein kinase 1 (PKABA1), which is a serine– threonine type protein kinase, was isolated from...
Ngày tải lên: 14/02/2014, 12:20
Tài liệu What to do after a death in England or Wales pptx
... and belongings 39 The will 39 Jointly-owned property 40 Getting permission to deal with the estate 40 What does the executor or administrator need to do? 42 Distributing the estate and dealing with ... will appoint an administrator to deal with the estate. The administrator will usually be someone who is a beneficiary of the will, or who is entitled to inherit under the intestacy rules. What to ... administrator, your role is to administer the estate. 40 Jointly-owned property Two or more people may own the home together as beneficial joint tenants or tenants in common. ã Beneficial joint...
Ngày tải lên: 16/02/2014, 10:20
Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf
... thought by providing just such an interdisciplinary forum, in which research results, including novel concepts such as environmen- tally induced increases in mutation rates in bacteria and a ... gene regulons and regulatory pathways involved in freezing tolerance in Arabidopsis. Freezing tolerance increases in many plants in response to low, non-freezing temperature, a process known as ... profiling revealed 57 transcripts specifically upregulated in acclimated plants, including heat shock proteins, transcription factors and immunophilins, as well as downregulated transcripts, including...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Dissociation of DNA polymerase a-primase complex during meiosis in Coprinus cinereus pptx
... proteins. Immunostaining of meiotic C. cinereus nuclei Immunostaining of meiotic C. cinereus nuclei was perfomed as described in previous reports [29–31] with minor modi- fications. C. cinereus ... needs to catalyze the incorporation of 1 pmol of [ 3 H]-d TTP into a DNA polymer in 30 min. Protein concentrations were determined using the Coomassie Brilliant Blue binding technique. ND, not ... was found in the meiotic prophase I including non-S phase stages, in the basidiomycetes, Coprinus cinereus. To study DNA poly- merase a during meiosis, we cloned cDNAs for the C. cinereus DNA...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx
... due to long range coupling involving vicinal isoprenoid moieties. Due to the line broadening, the precision of signal integration is substan- tially reduced. However, within the experimental limits, ... the two competing deprotonation paths is illustrated in Fig. 8 for the predominant ES complex A of a sample of IPP carrying a 13 C label in its methyl group; a similar scheme involving a less stable ... known precursor of the vitamins thiamine [11] and pyridoxol [12], could be incorporated into terpenoids by E. coli [9] as well as by higher plants [7]. More specifically, plants were shown to utilize...
Ngày tải lên: 22/02/2014, 07:20
Uptake of organic chemicals in plants Human exposure assessment ppt
... variance in 141 the measured concentration in plants is explained by the variance of the 142 concentrations in soil. 143 In case of an insignificant correlation, the concentration in plant ... uptake of organic contaminants from soil by plants. The goal was to gain insight into both experimental data and predictive methods. Knowledge of uptake of con- taminants in plants is relevant for ... SPME sam- pling kinetics were studied for a number of sample types in order to (1) determine the sampling time needed to ensure equilibrium sampling, (2) select the best mode of sampling (static...
Ngày tải lên: 05/03/2014, 20:20
Báo cáo khoa học: Transmembrane helix 12 plays a pivotal role in coupling energy provision and drug binding in ABCB1 pot
... examined by intro- ducing cysteines at distinct positions in TM12 and assessing their accessibility to covalent modification with thiol-reactive probes. In order to determine how changes in the ... state intermedi- ate [22,24,25]. Fluorescence labelling was subsequently car- ried out as detailed in the preceding paragraph. The extent of labelling was determined by examining the gel using ... transitions during the catalytic cycle. The previous section outlined the overall topog- raphy of TM12, by examining the accessibility of introduced cysteines to maleimide-containing probes. The...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants potx
... forma- tion by introducing the oxygen between carbon 9 and 10 of oleic acid before incorporation of the monomer into the cutin. In this context, it is interesting to note that inhibition studies ... encoding a fatty acid x-hydroxylase, a putative epoxide hydrolase, several genes encoding enzymes involved in the synthesis of fatty acids in plastids, including the stearoyl acyl carrier protein ... represents a highly diversified set of heme-containing proteins found in bacteria, fungi, animals and plants [7]. In animals, members of the CYP4A gene subfamily mainly cata- lyze the formation of x- and...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf
... indicating the presence of two catalytically independent domains, the N-terminal domain, display- ing ALDH activity, and the C-terminal domain, con- taining an iron-binding domain, which is involved ... 3387 extract during the O 2 exposure was involved in EhP- FOR inactivation (and hence avoiding the arbitrary selection of ROS-testing concentrations), the samples were incubated in the O 2 -saturated ... present during the incubation (data not shown). As Cat only partially prevented enzyme inactivation, EhPFOR inactivation by H 2 O 2 was examined in detail. The enzyme was strongly inhibited in a...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo khoa học: Improvement of ecdysone receptor gene switch for applications in plants: Locusta migratoria retinoid X receptor (LmRXR) mutagenesis and optimization of translation start site pptx
... DNA-binding domain with CfEcR ligand-binding domain cloned under the control of the 35S promoter in the pKYLX80 vector LmRXR A fusion of VP16 activation domain and LmRXR ligand-binding domain cloned ... Arabidopsis plants were selected by germinating the seeds collected from the inltrated plants on medium con- taining 50 mgặL )1 kanamycin. The analysis of transgenic plants for luciferase induction ... Kozak sequence. Interestingly, an increase in the ligand- induced activity of this switch containing the improved Kozak sequence was not accompanied by an increase in background activity in the absence...
Ngày tải lên: 15/03/2014, 23:20
Báo cáo khoa học: Photosynthetic acclimation: State transitions and adjustment of photosystem stoichiometry – functional relationships between short-term and long-term light quality acclimation in plants ppt
... reduction in seed production, indicating synergistic effects when both kinases are lacking [9]. Additional interesting results were obtained by some gene-expression studies. The lack of LTR in the ... ferre- doxin ⁄ thioredoxin system (Fig. 3). The interplay between the kinases and their substrates integrates these varying redox signals and initiates appropriate molecular acclimation responses in ... within the canopy or stand. In these layers, mainly high light intensities, which may harm the photosynthetic apparatus, must be counteracted, for example by NPQ. The incident light intensity in the...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants pdf
... of foreign genes in plants. Gene 61, 1–11. 44 Tavva VS, Collins GB & Dinkins RD (2006) Targeted overexpression of the Escherichia coli MinC protein in higher plants results in abnormal chloroplasts. ... Arabidopsis plants were transformed using the whole plant-dip method [42]. Transgenic Arabidopsis plants were selected by germinating the seeds collected from the inltrated plants on a medium containing ... expression in the absence of methoxyfenozide was indistinguishable from the back- ground readings detected in the transgenic plants that were transformed with a two-hybrid gene switch con- taining wild-type...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Cytochrome P450 metabolizing fatty acids in plants: characterization and physiological roles pptx
Ngày tải lên: 22/03/2014, 16:21