well defined flow and cell visualization

... BiFC signals and CT-B staining in live cells (C) Gag-VN/IMP1-VC BiFC signals and CT-B staining in live cells (D) HeLa cells or (E) Jurkat T cells were co-transfected with pCMV-Rev and Rev-dependent ... associations [13,58,59], vRNA encapsidation [49] and Gag trafficking Methods Cell culture and transfections HeLa cells and Jurkat T cells were maintained under standard conditions at 37°C with in Dulbecco's ... min) The cells were then fixed, stained for Caveolin-1 and Gag and visualized by laser scanning confocal microscopy (C) Hela cells were co-transfected with pCMV-Rev and either Gag-VN and Staufen1-VC...

Ngày tải lên: 12/08/2014, 23:23

... microspheres, and (e) and (f) Ag@air@P(MAA-co-DVB)-click-PNIPAM hybrid microrattles Figure 5.22 XPS wide-scan and C 1s core-level spectra of the (a and b) Au@SiO2@P(MAA-co-DVB), and (c and d) Au@SiO2@P(MAA-co-DVB)-click-PEG ... by Figure 3.1 FESEM and TEM micrographs of (a) and (b) 3-(trimethoxysilyl) propylmethacrylate-silica (SiO2-MPS) seeds, (c) and (d) SiO2-PMAA core-shell microspheres, and (e) and (f) SiO2-PMAA-SiO2 ... PMAA hollow microspheres, and (e) concentric hollow silica microspheres Figure 3.4 FESEM and TEM micrographs: (a) and (b) pH-responsive PMAA hollow microsphere, and (c) and (d) concentric hollow...

Ngày tải lên: 10/09/2015, 08:40

... bearing Strand A or strand B Lane P1 and Q1 correspond to the conjugates bearing strands of digested Strand A and Stand B respectively; Lane P2 and Q2 correspond to conjugates bearing strands of ... bearing Strand A Lanes A1, B1 and C1 correspond to EcoR V digested conjugates bearing 4, and strands of Strand A respectively; Lanes A2, B2, C2 correspond to the conjugates bearing 4, and strands of ... EcoR V digested conjugates bearing 4, and strands of Strand B respectively; Lanes I2, J2 and K2 correspond to the conjugates bearing 4, and strands of Strand B without EcoR V digestion respectively;...

Ngày tải lên: 12/09/2015, 11:29

... macromolecule with a brush-shaped hydrophilic head (rod) and a hydrophobic tail (coil) The formation of well- defined and uniform micelles from the present well- defined block-graft copolymers was also demonstrated ... with well- defined molecular weights and the study of the micelle formation in these materials will be of great interest The fluoropolymers and copolymers with well- controlled molecular weight and ... by AFM The formation of well- defined and uniform micelles from the present well- defined block-graft copolymers was demonstrated CHAPTER LITERATURE REVIEW 2.1 Applications and Physicochemical Properties...

Ngày tải lên: 16/09/2015, 08:31

... the velocity and temperature of the flow field are solved employing perturbation technique and the effects of the flow parameters on the velocity and temperature of the flow field and also on ... velocity and temperature of the flow field and also on skin friction and the rate of heat transfer at the wall The effect of growing permeability parameter is to accelerate the velocity of the flow ... scholars and presently guiding 15 Ph D scholars Now he is carrying on his Post Doc Research in MHD flow through Porous Media His major fields of study are MHD flow, Heat and Mass Transfer Flow through...

Ngày tải lên: 05/09/2013, 14:58

... suppression and cell mortality Cell 85:27–37 Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW (1997) Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a Cell ... human fibroblasts by p16 and the polycomb protein Bmi-1 Mol Cell Biol 23:389–401 Itahana K, Campisi J, Dimri GP (2004) Mechanisms of cellular senescence in human and mouse cells Biogerontology 5:1–10 ... in cultured mouse cells p19ARF is predominantly upregulated during senescence by oncogene activation and/ or oxidative stress Senescence and Cell Cycle Control 265 In human cells undergoing replicative...

Ngày tải lên: 25/10/2013, 21:20

... the daughter cells at the end of cell division and in cellular autolysis [9], where it mediates the release of toxins that damage the host tissues and allows the entry of pneumococcal cells into ... analysis of ligand binding to C-LytA (A) Conformational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM choline; ... analogs on cell growth and viability Fig Effect of choline and analogs on the activity of cell wall lytic enzymes Data are shown as percentage of activity with respect to nonligated enzyme, and are...

Ngày tải lên: 19/02/2014, 05:20

... lymph node, spleen, liver and lung, as well as in pancreatic acinar cells, adrenal gland, spermatogonia and spermatids of testis, and in Purkinje cells and in the granular layer of cerebellum ... endothelial cells, pancreas, liver astrocytes Acts on activated T cells Acts on lymphocytes, dendritic cells, haematopoetic cells LD78b (CCL3 ⁄ L1) Eotaxin (CCL11) Expressed by T cells, B cells and monocytes ... recruitment of CD26 and CD45 to these rafts [80] and induces T -cell activation DPIV family in cancer and cell biology Fig Features of the effects of DP upon T -cell activation ⁄ function Cell- surface...

Ngày tải lên: 06/03/2014, 09:22

... incomes, and broadband and wireless users—report more frequent chance encounters with news as well as more frequent hunting and gathering experiences The youngest adults (those under age 30) and cell ... groups: women, African-Americans, and older Americans (those 65 and older) By comparison, those who are internet users and those who have a cell phone but no landline are less likely to get local ... the cell- only population (those who have dropped their landline and rely exclusively on their cell phone) get radio news on a typical day, compared with 39% of those who rely exclusively on landlines...

Ngày tải lên: 06/03/2014, 21:20

... were chosen, and their total wing area, anterior (A) and P compartments area and cell density were measured (see Experimental procedures) Cell size was then estimated as the inverse of cell density ... from defects in cell adhesion and ⁄ or cell communication mfl silencing triggers apoptosis and sorting out of D cells towards the V compartment To assess the specific effects on cell apoptosis, ... cells expressing different levels of Mfl or still unexplained cell nonautonomous effects, such as defects in cell communication and ⁄ or cell affinity In order to evaluate the growth of mfl 05 cells...

Ngày tải lên: 06/03/2014, 22:21

... translocation experiments, cells were removed with 0.05% trypsin EDTA, and plated with cell solution of ã 106 cellsặmL)1 (in a 50 mL ask) and grown until 80% conuency Cellular assays A549 cell solution (100 ... sources Cell culture A549 cells (human lung carcinoma epithelial cell line SNB0000178-CE A549) were passaged every days in DMEM (+ mm l-glutamine and 5% fetal bovine serum) and maintained at 37 C and ... regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report the results of cell- based, in vitro and in silico experiments...

Ngày tải lên: 16/03/2014, 11:20

... control and mutant DNAzyme-treated cells (Fig 5) Inhibition of Saos-2 cell invasion by Dz720 The plasminogen activator system can promote the breakdown of ECM and facilitate cell invasion and metastasis ... controls (P < 0.01) (Fig 6) Saos-2 cells treated with the active Dz720 had 42% and 17% fewer cells on the lower side of the filter compared with untreated control cells and cells treated with Dz720 mutant, ... uPA and uPAR to further inhibit cancer cell invasion and metastasis complex, the medium was replaced with DMEM containing 10% fetal bovine serum Cells were collected and assayed by northern and...

Ngày tải lên: 16/03/2014, 22:20

... eastern Massachusetts Fine to coarse sand and gravel Silt and clay Sand and gravel Medium to coarse sand and gravel Sand and gravel Fine to coarse sand and gravel AN-11G BSC1 ARNWR2 CN-01G HD-01G ... which it occurs and the timing and quantity of recharge Impoundments, ponds, and wetlands, as well as climate and topography, affect surface-water flow Ground-water- and surface-water -flow systems ... for medium to coarse sand and gravel, 0.21 to 0.33 for fine sand, and 0.02 to 0.08 for silt and clay (Johnson, 1967; Morris and Johnson, 1967; Moench and others, 2000; Kontis and others, in press)...

Ngày tải lên: 17/03/2014, 15:20

... with glands in the fundus and body that produce mucus (surface and neck cells), pepsinogen (chief cells), and acid and intrinsic factor (parietal cells) Intrinsic factor binds to vitamin B12 and ... (humoral immunity), T lymphocytes (cellular immunity), macrophages (phagocytic and one of several types of antigen-presenting cells), and mast cells Immunity and T and B cells There are two types of ... infection and release histaminases to counteract basophils and mast cells; and basophils, which contain histamine and heparin and assist mast cell function Bone marrow is the site of blood cell development...

Ngày tải lên: 22/03/2014, 21:20

... glutamine, cysteine, and glycine It is the most abundant intracellular small molecule thiol present in mammalian cells and it serves as a potent intracellular antioxidant protecting cells from toxins ... 28 Targeting New Pathways and Cell Death in Breast Cancer Despite its well- documented association with transformation of normal breast epithelial cells and metastasis and poor outcome of breast ... tumor suppressors p53 and PTEN, and cell cycle inhibitors p21 Cip1/WAF1 and p27Kip1 (Park et al, 2008) Whereas the molecular machinery utilized by HER2 to promote cell migration and invasion remains...

Ngày tải lên: 23/03/2014, 17:20

... Daniels MA (2009) ERK and cell death: ERK location and T cell selection FEBS J Martin P & Pognonec P (2009) ERK and cell death: cadmium toxicity, sustained ERK activation and cell death FEBS J Taylor ... ⁄ and MEK5 in those cells FEBS Journal 277 (2010) 2–21 ª 2009 The Authors Journal compilation ª 2009 FEBS 13 ERK and cell death S Cagnol and J.-C Chambard Fig The hallmarks of ERK-mediated cell ... of cell death that correlated with extensive cell rounding and the formation of cytoplasmic macrovacuoles, which pushed the nucleus and the cytoplasm to the side of the dying cell Although cell...

Ngày tải lên: 29/03/2014, 08:20

... membrane damage and independently of caspase-3 J Cell Biol 165, 357–369 35 Mebratu Y & Tesfaigzi Y (2009) How ERK1 ⁄ activation controls cell proliferation and cell death: is subcellular localization ... hand, in non-neuronal cells, cytoplasmic retention of ERK1 ⁄ is required for death-associated protein kinase-mediated cell death [35] Thus, ERK1 ⁄ might promote cell death depending upon the cell ... multiple activators and targets, it is possible that activation and its cell death-promoting function may involve multiple partners and regulations [5] ERK1 ⁄ in neurodegeneration and ischemia ERK1...

Ngày tải lên: 29/03/2014, 08:20

... problem Interestingly, JNK activity and subcellular location are the same regardless of the ligand strength, ERK location and T cell selection whereas ERK activity and location change depending of ... a cell It was recently shown, that in thymocytes, positive and negative selecting ligands induce the localization of the components of the Ras ⁄ ERK cascade and active ERK into distinct subcellular ... negative selecting ligand Note the separate location of pJNK and pERK, and Ras-GRP1 ⁄ Grb2 ⁄ SOS ⁄ Ras ⁄ Raf at the plasma membrane lead to such distinct cell fates as survival and death Much is...

Ngày tải lên: 29/03/2014, 08:20