wax from stems and branches of euphorbia cerifera

... of gelatin; production of lecithinase; and a positive reverse CAMP test RESULTS AND DISCUSSION result Also, H2S production properties of 3.1 Prevalence of C perfringens isolates from cattle and ... Genotyping of Clostridium perfringens isolated from cattle and pigs with diarrhea in Hanoi and surrounding areas, Vietnam 3.2 Genotyping of C perfringens the toxicity of C perfringens isolates and the ... Typing of isolates of Clostridium perfringens from healthy and diseased sheep by multiplex PCR Vet Med - Czech 50, 439-442 631 Genotyping of Clostridium perfringens isolated from cattle and pigs...

Ngày tải lên: 29/08/2013, 08:14

... elution using ml of methanol, 10 µL of the methanol eluent was added to 10 ml of CB medium (Watanabe and Satake, 1991) in a test tube, and after that M aeruginosa - 56 - Journal of Water and Environment ... metabolites produced by plants and other organisms that influence the growth and development of biological and agricultural systems (Marick, 2005) Since certain kinds of macrophytes have been reported ... (Oryza satives) seedlings by water extracts of C rotundus and leachate of its leaves, and found the existence of phenolic compounds such as 4-hydroxy benzoic and 3,4-dihydroxy benzoic acids Additionally,...

Ngày tải lên: 05/09/2013, 09:38

... composition of silt and clay The low recovery rate was also because of the inhibition of nucleic acids extraction and PCR by humic substances extracted from sediment by high pH as Tsai and Olson ... (1996) and Watson and Blackwell (2000) reported The color of supernatant after vortex mixing and centrifugation was brown and that of the concentrate after membrane filtration was yellow, both of ... directly from 0.5 g wet sediment samples using ISOIL for Beads Beating (Nippon Gene, Tokyo) Detection of pathogens and fecal indicators by PCR and qPCR methods The concentration of (c)DNA of Poliovirus...

Ngày tải lên: 05/09/2013, 10:15

... (mg/L) (mg/L) of Ca and Mg added Amount of Ca and Mg added (mg/L) (a) (b) Adsorbed Ca, Mg (mg/g) Fig - Concentration profiles of (a) As and (b) Fe in leachate with the addition of Ca and Mg in different ... (mg/g) a average ± standard deviation of samples However, upon addition of 100 mg/L each of Ca and Mg leaching of As decreased to 24.6 and 23.3 µg/L, respectively Leaching of As was decreased ... < 11.5 and might form at pH 12 and 12.5 Leachate pH obtained ranged between 7.0 and 8.0 which might not support the first hypothesis On the other hand, adsorption of Ca and Mg from GW and subsequent...

Ngày tải lên: 05/09/2013, 10:15

... expanded since the discovery of the photocatalytic properties of TiO2 and the demonstration of its effectiveness to generate hydroxyl radicals in the presence of UV light [8] The more activity of ... treatment using nanoparticles of TiO2 and UV light The main feature of this work is the use of inexpensive and recoverable catalyst This sort of processes for degradation of organic compounds may ... black run) and with UV light (both with 100 mg L−1 of TiO2, and temperature of 303 K) Figure shows, very low reduction of COD which was found in the absence of light The increase of temperature...

Ngày tải lên: 05/09/2013, 17:03

... mail service, on the other hand, is a large, complex piece of software that accepts data (mail) from and returns data to the client, as well as reads and stores data and configuration information ... Programmability and Configurability—Low Standard Unix Services Unix has a bevy of simple legacy services from early in the development of the Internet—when security was less of an issue and it was ... Internet name is not proof−positive of hacking, it is indicative of a domain with lax administration If the protocols you support allow it, and your network can handle the overhead of looking up every...

Ngày tải lên: 29/09/2013, 13:20

... Do's and Don'ts for More Professional Speaking and Writing Tips and tricks gleaned from UGA’s Foundations of Leadership and Management Program Business Skills Quiz Sixty-seven percent of first ... with his/her hands for the most energy and sense of professionalism? a b c d A A It A speaker should keep hands clasped behind their back speaker should keep hands clasped in the front of their body ... Dark-colored suits (for both men and women) convey a sense of authority and power Managing a Meeting           Do create an environment of equality Stand to shake hands when someone walks into...

Ngày tải lên: 20/12/2013, 23:15

... Norman and Angevin kings, is the foundation of the common law of England, and the common law of England is the law of more than half of the civilized world Whatever the varieties and distinctions of ... hordes; of the long fight between popes and emperors over the right of investiture; of Rudolph of Hapsburg; of the throwing off of their allegiance to the Empire of the Kings of Burgundy, Poland, ... Palatine of the Rhine, the Duke of Saxony, the Margraf of Brandenburg, and the three Archbishops of Mayence, Treves, and Cologne; of the independence of the great cities of northern Italy; of Otto...

Ngày tải lên: 16/02/2014, 04:20

... classified correctly in 85% of cases and Table Effect of cut-off value on the prediction accuracy The prediction accuracy, specificity, sensitivity, number of mutants classified and MCC values on training ... shown using difference sizes of the safety margin By increasing the safety margin, we can go from 77% accuracy and an MCC value of 0.52 to 88% accuracy and an MCC value of 0.74 The drawback is that, ... From Table 10, it can be seen that p53R2 possesses a few mutants that behave differently from the rest of the promoters Of these, amino acid residues 243 and 275 are involved in DNA binding and...

Ngày tải lên: 18/02/2014, 11:20

... concentrated and used for biochemical analysis SDS/PAGE of purified BHs Fig Effect of camalexin and 3-phenylindole on the activity of BH from mycelial cultures of Leptosphaeria maculans (A) and Alternaria ... 85% inhibition of BHLmL2 and BHAb, respectively, while mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively The influence of pH on the activities of the BH enzymes ... BHLmL2 for the BH from L maculans L2 ⁄ M2, and as BHAb for the BH from A brassicicola Table summarizes the purification procedure used for BHLmL2 and indicates the degree of purification and yield achieved...

Ngày tải lên: 18/02/2014, 14:20

... BMP-R-Smad ortholog from S mansoni Fig Structure of the SmSmad1B gene, cDNA and protein (A) Schematic representations of the SmSmad1B gene, cDNA and protein and the amino acid sequence of SmSmad1B protein ... properties of SmSmad1 and SmSmad1B as described above However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of ... 4087 SmSmad1B, a BMP-R-Smad ortholog from S mansoni J M Carlo et al enhanced the interaction of SmSmad2 and SmSmad4 and resulted in the formation of a stable and functional Smad complex [6,7] The...

Ngày tải lên: 18/02/2014, 16:20

... studies, high affinities and activities of CA had been found in the plume and in the trophosome CA from the branchial plume tissue had an affinity of 13.9 mmolÆL)1 and an activity of 253.7 lmol CO2Æmin)1Æg)1 ... (RpCAbr and RpCAtr) not appear to be membrane-bound isoforms The RpCAbr and the RpCAtr transcripts are phylogenetically related and both distant from the vertebrate membrane-bound (CAIV) isoforms, and ... downstream from the in-frame stop codon and 19 nucleotides upstream from the poly(A) tail Search of motifs with the PROSITE server (ScanProsite) [18] showed the presence of an a-CA signature from amino...

Ngày tải lên: 18/02/2014, 16:20

... exemplified by a comparison of the catalytic-site structure and characteristics of cathepsin B and papain pH-dependent kinetics of the reactions of cathepsin B from bovine spleen and from rat liver with ... The overall structure and fold of ervatamin-A is similar to that of ervatamin-B, ervatamin-C [27,35] and other members of the papain family, and is made up of two domains, L and R, with a V-shaped ... rounds of positional refinement, fitting, and introduction of water molecules and SO42), followed by manual fitting of the model by quanta (Accelrys Inc.), led to an R-factor of 19.78% and Rfree of...

Ngày tải lên: 18/02/2014, 16:20

... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... mistletoe lectin I [viscumin] from Viscum album [7], modeccin [8] and volkensin [9] from the roots of Adenia digitata and Adenia volkensii, respectively, and pulchellin from Abrus pulchellus seeds...

Ngày tải lên: 18/02/2014, 16:20

... X Huo et al site involving heme a3 and CuB by means of protons extracted from the matrix side of the mitochondrial inner membrane and electrons from the cytochrome c complex, in a reaction ... Characterization of respiratory complex II Fig Sequencing of porcine heart mitochondrial complex II Total RNA was extracted and purified from the fresh porcine heart, and genes of four complex ... G250, and the four bands corresponding to subunits of porcine complex II were cut out and N-terminal sequenced by the Edman degradation method and amino acid analyzer in the Department of Biology,...

Ngày tải lên: 19/02/2014, 02:20

... disappearance of the Soret band, and the quantity of bilirubin was determined from the A468 with e468 ¼ 55 mm)1Æcm)1 [43] Detection of CO To each solution of heme–GmHO-1 (5 lm) and FNR ⁄ Fd (0.22 and lm, ... Soret band maxima of the azide-free form at 405 nm and of the azide-bound form at 421 nm, so the relative amounts of the nonbound and azide-bound forms were estimated directly from the values of ... time, indicating the formation of biliverdin In this case, neither bands of the oxy form (at 541 and 578 nm) nor bands of verdoheme and CO–verdoheme (at  690 and at  640 nm, respectively) are...

Ngày tải lên: 19/02/2014, 05:20

... Da) The identity of this peptide was then confirmed by MALDI-TOF ⁄ TOF fragment ion analysis, where partial sequences of this peptide were obtained from the ladders of b-fragment and y-fragment ... The met state is the resting state of the enzyme, and in the absence of substrate about 85–90% of the enzyme is in this state [16] Both the met and oxy states of tyrosinases can catalyze the diphenoloxidase ... bottom of a large vacant space and one of the six histidine ligands appeared to be highly flexible, was determined with a help of a caddie protein, ORF378, at ˚ 1.2–1.8 A resolution [17] Knowledge of...

Ngày tải lên: 19/02/2014, 06:20

... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O) Panel M is the control ... Figure 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nucleus, and Fig 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respectively ... sequencing and digestion with NcoI and EcoRI to give a band of 678 bp (Fig 6E, lane 2) On the other hand the complete ORF of PsCIPK (1.5 Kb) was cloned into the EcoRI and XhoI sites of yeast two-hybrid...

Ngày tải lên: 19/02/2014, 07:20

... deletion mutants of the 3¢ terminus of the minus-strand RNA but deletions were made on the basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minusstrand RNA being ... Binding and replication of 3¢-end of HCV minus RNA Fig Secondary structure of RNA mutated in the SL-A1 and SL-B1 domains Only the secondary structure of the 151 nt from the 3¢-end of the minus-strand ... 3873 Binding and replication of 3¢-end of HCV minus RNA T Astier-Gin et al Fig Secondary structure of the 3¢ terminal sequences of HCV minus-strand RNA Model structures of domain I (A) and domain...

Ngày tải lên: 20/02/2014, 01:20

... mapping On the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productive complex is independent of the length of the substrate, Km and Vmax were used ... pET24d from Novagen (Madison, WI, USA) PGA was obtained from ICN (Zoetermeer, the Netherlands) Saturated oligoGalpA (DP 28) and unsaturated oligoGalpA (DP 37) were prepared and puried from polygalacturonase ... values A-1 and kint were derived from a plot of exp(An+1 RT) against (1 kcat)n: expAnỵ1 =RTị ẳ ẵkint =kcat ịn expA1 =RTị in which A-1 and kint are determined from the vertical and horizontal...

Ngày tải lên: 20/02/2014, 03:20