watermarking an image and the concept of testability seams

... party in the browser’s Referer header Membership, Roles, and Profiles Another one of the great conventions of the Web is user accounts Where would we be without them? Then there’s all the usual ... using IIS and some of these authentication mechanisms aren’t available, you’ll need to enable them on your server Go to Control Panel ® Programs and Features ® “Turn Windows features on and off” ® ... expiration and memory management facilities already built in, and your controllers can easily access an instance of it via HttpContext.Cache You will probably want to use Cache for the results of any...

Ngày tải lên: 06/08/2014, 08:22

... discussion and Patrice Bouchard and Betty Zhu for technical assistance We also thank Andy Ng and Evelyne Copeland for critical reading of the manuscript This work was supported by a Genomics and Health ... a constant dependent on the properties of the ligand, [L]T is the total ligand concentration, [P] is the concentration of free SH2 protein, and Kd is the equilibrium dissociation constant Average ... Initiative of the National Research Council of Canada (NRCC Publication No 46198), sponsored by the Government of Canada References Kullander, K & Klein, R (2002) Mechanisms and functions of Eph and...

Ngày tải lên: 19/02/2014, 12:20

... of the donor and a reference compound, and ARF and AD are the respective absorbances of the reference compound and donor at the excitation wavelength QRF is the quantum yield of the reference ... change in Trp13 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used ... increase in the uorescence quantum yields from the value of 0.09 for apo-CRP to the value of 0.094 for the CRP(cAMP)2 complex, and no substantial changes in the shape of the emission spectra of the donor...

Ngày tải lên: 20/02/2014, 23:20

... rather than the substrate per se To better understand why these analogues have high affinity and to help in rational drug design, we undertook a thermodynamic characterization of substrate and analogue ... brucei 6PGDH The first observation focuses attention on the role of entropy and the phosphate group The major contribution to the binding energy of 6PG and its analogues comes from entropy and, in ... low and positive, 0.174 kcalÆ mol)1, and the binding is totally entropy driven The buffer-independent enthalpy change for the binding of 5PR and 4PE is negative (Table 1), with the release of...

Ngày tải lên: 07/03/2014, 05:20

... curve The plots of the inverse time constant (kobs) are linear (Fig 9) and the values of koff and the association-rate parameter, kon, listed in Table 3, were determined as the intercept and the ... Cys178 of CRP and uorescently labeled DNA sequences (Fig 1) The mechanism of the cAMP-induced long-distance structural communication within the CRP remains an important part of our understanding of ... as [CRPAEDANS] An equilibrium binding constant can be calculated from the ratio of the rate constants kon and koff as follows: Ka ẳ kon koff 2ị Association constants (Ka) of CRP with the three...

Ngày tải lên: 07/03/2014, 16:20

... complexes in the absence of drugs (A) and presence of abacavir (B), nevirapine (C) and atazanavir (D), at pH 7.0 and 25 °C In all panels, the binding isotherm measured in the absence of either drugs ... complexes in the absence of drugs (A) and in the presence of abacavir (B), nevirapine (C) and atazanavir (D), at pH 7.0 and 25 °C In all panels, the NMRD profile measured in the absence of either drugs ... drugs with the concomitant intoxication of the patient [7,35,51] Moreover, binding of drugs to FA2 reduces the affinity of FA7 and increases the affinity of FA1 ligands Eventually, FA6 ligands are...

Ngày tải lên: 23/03/2014, 07:20

... kobs(rapid) and kobs(slow) are the observed association rate constants for the rapid and slow association phases, respectively The values for the constants are means ± standard deviations of three ... Characterization of the binding of PCSK9 to intact cells S A Mousavi et al Fig Dissociation time courses and effects of unlabeled ligands and low pH on the dissociation rates of [125I]TC-PCSK9 (A) and [125I]TC-PCSK9-D374Y ... dissociation, but also of the endocytic rate constant, and measurements of these rate constants require a method to discriminate between the surface-bound and internalized ligand [43] However, an accurate...

Ngày tải lên: 14/02/2014, 14:20

... filtration and dynamic light scattering of the apo and holo forms of bA3- and bB2-crystallins There was no significant change in the stability and hydrodynamic radius of the both forms of proteins ... proteins and induce luminescence peaks at 492 nm and 547 nm via energy transfer from Trp and Tyr residues [32] Terbium binds to bB2- and bA3-crystallins and induces luminescence peaks at 492 and 547 ... indicating the contribution from aromatic amino acids and Cys (there are Trp, Tyr, Phe and Cys in bB2-crystallin and Trp, 11 Tyr, Phe and Cys in bA3-crystallin) (Fig 8) There is no significant change...

Ngày tải lên: 18/02/2014, 16:20

... acid binding and transport properties of HSA [1–8] Fatty acids (FAs) are required for the synthesis of membrane lipids, hormones and second messengers, and serve as an important source of metabolic ... G Fanali et al responsible for the transport of lipophilic compounds and drugs and of medium and long chain fatty acids; among them, myristic acid is a stereotypic ligand to investigate ... on the conformational state of HSA and on the occupancy of the myristate binding sites The relaxation rate change is highest between pH 5.5 and 8.0, where HSA is in the native form (N state) The...

Ngày tải lên: 20/02/2014, 02:22

... the effect of divalent cations on the binding of ICAM-4 transfectants to the I domains of CD11a and CD11b and compared the results to the divalent cation requirements of ICAM-1 and ICAM-2 transfectants ... to the ICAM-1 transfectants The presence of both MgCl2 and CaCl2 gives better binding of ICAM-2 L cell transfectants to the I domain of CD11b as well as for adhesion of ICAM-4 transfectants and ... per well (B) Shows the effect of anti-ICAM-4 (BS46 and BS56) and anti-CD11a I domain (TS1/22 and MEM83) mAbs on the binding of ICAM-4 positive and negative red cells to lg of coated CD11a/CD18...

Ngày tải lên: 20/02/2014, 11:20

... 36% of the interaction of the full peptide with the binding cavity of SM3 The sizes of the surfaces were determined using distances between ligand ˚ atoms and protein atoms of less than A These ... from the 1D spectrum 25% relative intensity in the STD spectrum Obviously, Pro1 and Asp2 get more saturation from the protein than the remaining residues of the ligand and therefore have more and ... consequence of this exchange phenomenon there are no NOE contacts between Pro1 and Asp2 This segment of the ligand is thus ill defined and was manually adjusted to fit the X-ray structure of the peptide...

Ngày tải lên: 21/02/2014, 15:20

... [20]: koff ẳ koff2ị ẵibuprofen=K2 ẵibuprofenị ỵ koff3ị ẵibuprofen=K3 ẵibuprofenịị ỵ kỵ off 7ị where koff(2) and koff(3) indicate values of koff occurring at K2 < [ibuprofen] < K3, and at ... K3 < [ibuprofen], respectively, and kỵ is the koff value obtained in the off absence of ibuprofen Data analysis Kinetic and thermodynamic data were analyzed with the matlab program (The Math Works, ... study and [20,21]) This indicates that the removal of domain I from HSA does not signicantly affect the functional and structural properties of domains I and II [i.e of the 1382 region of (t)HSA]...

Ngày tải lên: 06/03/2014, 01:20

... described in the secretion, stability and oligomerization of proteins [38,39], the bioactivities of enzymes [40] and the binding affinities of ligands and receptors [41] In many instances, specific ... response We propose that glycosylation is important for the folding of HFE and is essential for transport and exit of the protein from the ER The importance of glycosylation is cumulative, however, ... successfully transit the ER HEK293T cells were transfected to express wild-type or mutant forms of the HFE protein Cells were lysed and an anti-b2M Ig used to immunoprecipitate b2M and any interacting...

Ngày tải lên: 06/03/2014, 22:21

... GST-N and GST-C34, an hCaM band is only seen in the presence of Ca2+ (lanes 5, and 9, 10) In the absence of Ca2+ (lanes 3, and 7, 8) and for the GST control (lanes and 2) there was no retention of ... [21–25], and voltage-gated channels of the KCNQ [26] type are known to interact with CaM hEAG1 channels studied here share some properties of CNG channels and of KCNQ channels On the one hand they ... hCaM-EF34 and hCaM-EF1234) EF-hand mutants of hCaM were also employed for functional tests on hEAG1 channels expressed in Xenopus oocytes The channel-inhibiting potency of wildtype hCaM and the EF-hand...

Ngày tải lên: 07/03/2014, 12:20

... II; HP, structure of human plectin and 58 solvent molecules in the asymmetric unit and in the Ramachandran plot there were 88.2% of the residues in the most favored region and 11.8% in additionally ... structure and the corresponding subdomains of the A and B molecules of utrophin and dystrophin probably have not arisen by chance and may have significance for functional properties of these ABDs The ... in which the CH1 and CH2 subdomains were facing each other through helices a1 and a5, and helices a10 (including the subsequent loop) and a11 Molecules A and B in the asymmetric unit of the pleABD/2a...

Ngày tải lên: 07/03/2014, 15:20

... of porcine odorant binding protein with halothane and isoflurane are shown in Figs and Each peak in the binding isotherm (upper panels, Figs and 5) represents a single injection of halothane and ... injection of anesthetic was plotted vs the anesthetic ⁄ porcine odorant binding protein molar ratio (lower panels, Figs and 5), and the DH°, Kd, the free energy change associated with binding (DG°), and ... mechanism of volatile general anesthetic action Results Binding of the volatile anesthetic halothane to the hydrophobic core of porcine odorant binding protein The binding of halothane to the...

Ngày tải lên: 07/03/2014, 16:20

... concentration of the proteinligand complex, and [P] and [L] are the concentrations of the free protein and free ligand (i.e inhibitor), respectively [68] Expressed in terms of r, dened as the number of ... which IP and IPLn are the intensities of a free protein and different proteinligand complexes summed over the charge states and the isotopic distributions The free ligand concentration was then: ... as a function of the free ligand concentration and the t to Eqn (3) (solid line), obtained in the case of TRX I and S-Xyl5-Me On the basis of the Ka obtained, one can calculate the binding free...

Ngày tải lên: 07/03/2014, 17:20

... determine the accuracy of the model Usually, the given data set is divided into training and test sets, with training set used to build the model and test set used to validate it © Tan,Steinbach, ... collection of records (training set ) – Each record contains a set of attributes, one of the attributes is the class Find a model for class attribute as a function of the values of other attributes ... partitions, A < v and A ≥ v Simple method to choose best v – For each v, scan the database to gather count matrix and compute its Gini index – Computationally Inefficient! Repetition of work © Tan,Steinbach,...

Ngày tải lên: 15/03/2014, 09:20

... demonstrating the antagonism of the two effectors When the ratio of GTP to UTP was varied over the same range, the effects were similar to the effects of GMP and UMP; the values for the apparent ... and a definition of the specificity of nucleotide effects on RNA binding The implications of the the current findings for the physiological regulation of pyrimidine biosynthesis are presented; the ... at high and low protein concentrations and in the presence and absence of 0.1 m NaCl The results of the sedimentation velocity studies are summarized in the (supplementary Table S3) The calculated...

Ngày tải lên: 16/03/2014, 06:20

... specific interactions to the sugar and the base of the substrate The 3¢-oxygen of deoxyribose is hydrogen bonded to Tyr70 and Glu172, and the 5¢-oxygen is hydrogen bonded to Glu52 and Arg105 A key interaction ... to the loss of hydrogen bonds The substitution of the 3¢-oxygen with an azide group in AZT apparently destabilized part of the structure The only substitutions of the pyrimidine ring of the analogs ... Glu52 in the dNK–dTTP structure, as shown in Fig In the structure of the dGTP complex, the guanosine base occupies approximately the same geometrical space as the base in the dCTP and dTTP ligands...

Ngày tải lên: 16/03/2014, 06:20