vitamin e tocopherols and tocotrienols as potential radiation countermeasures

... D6desaturases (PtD5 and PtD6) were expressed in the yeast Saccharomyces cervisiae to determine their role in EPA biosynthesis and no ARA was recorded in this case While co-expressing both PtD5 and ... characterized the three cDNAs coding for 6-desaturase (MpDES6), 6-elongase (MpELO1), and 5-desaturase (MpDES5) from M polymorpha The presence of LA and ALA in the wild-type yeast Pichia pastoris encouraged ... the coexpression of elongase and D5-desaturase genes from M alpina in yeast could produce 1.32 lg endogenous ARA Seedspecific expression of D6, D5 desaturase and GLELO elongase genes from M alpina

Ngày tải lên: 14/01/2020, 01:14

... behavior and molecular biology of these cancers, comprehensive investigations are needed to guide the clinical management of UTUC In recent years, massively parallel sequencing has allowed insights ... these markers with overall survival using Kaplan-Meier model and Cox regression Results: Functional enrichment of significantly deregulated genes revealed that expression patterns of UTUC were ... using massively parallel sequencing, and identify potential molecular markers for prognosis in patients with UTUC Methods: We compared the genome-wide mRNA expression profile of cancer and matched

Ngày tải lên: 30/09/2020, 14:59

... singlet state less, and therefore their fluorescence is more intense 2.4 Photochemical properties Singlet oxygen may be determined by main methods: using chemical quenchers or using luminescence ... phthalocyanine derivatives is presented in this work The novel complexes were characterized by elemental analyses and spectral data such as infrared, nuclear magnetic resonance, ultraviolet visible, and ... Std are the respective absorbances of the samples and standard at the excitation wavelengths n2 and n2Std are the refractive indices of solvents used for the sample and standard, respectively ZnPc

Ngày tải lên: 12/01/2022, 23:24

... lipid peroxidation2, thereby helping plants to defend against various stresses and extending the shelf life of meat via keeping it fresh3 Furthermore, vitamin E deficiency has been shown to result ... normal conditions, as well as under NaCl and ABA treatments Meanwhile, the expression level of several genes associated with ABA biosynthesis (NCED3, NCED5 and NCED9) and the ABA signaling pathway ... β-tocotrienol and the total vitamin E content in Arabidopsis seeds Furthermore, these transgenic Arabidopsis seeds exhibited an accelerated germination time, compared with wildtype seeds under normal

Ngày tải lên: 04/12/2022, 15:54

... complete inactivation was observed (Figure 4A). The thermostability over time was examined at different temperatures in a 3-hour period. These proteases were highly stable at tempera- ture below ... storage of these enzymes was also investigated using dis- tilled water and 50 mM sodium phosphate buffer pH 7.5 as preservative solvents. The freeze-dried enzymes were dissolved in these solvents containing ... obtained in our study was expected to be higher because of the close evolutionary relationship between these earthworm species. Cho et al. reported extremely high conservation of the N- terminal

Ngày tải lên: 20/06/2014, 23:20

... represent the mean ± SE (mean standard error) of three independent experiments in triplicates. * Different capital letters denote statistical difference between viability in the different times ... phosphatidylserine. Data were normalized with the control (cells without treatment) and expressed as percentage of the mean ± SE of three experiments that were independently performed in triplicate. One or ... Nanoparticles and Magnetoliposomes SPIOs were obtained in the maghemit e (g-Fe 2 O 3 )phase and presented the characteristic diffraction patterns of inverse spinel structure when compared to reference

Ngày tải lên: 11/08/2014, 00:23

... confluence, FLSs were washed three times with phosphate-buffered saline (PBS) and cultured in 2 ml of serum-free DMEM for 48 h. Serum-free medium was used to exclude the contamination of miRNAs ... differences in analyzed miRNAs between RA and OA. However, radar charts of the mean concentra- tion of each miRNA showed the difference in secretion patterns of miRNAs between tissues (Figure 3D). ... verified that all the inserted size was approximately 60 nucleotides by electrophoresis, and that sequences were correct. These results were probably attributed to low abundance of pre-miRNAs and

Ngày tải lên: 12/08/2014, 12:20

... significantly reduced the infarct area, decreased the number of cells positively stained with anti-cleaved caspase-3 and anti-Cox-2 antibodies, suggesting that Luteolin might exert neuroprotective effects ... the shortest possible time from the onset of stroke, as well as to reduce ischemia-caused tissue damage Unfortunately, despite decades of research, alteplase (a tissue plasminogen ... equipment, and Dr Koh Hwee Ling for her kind agreement for me to use her Blood Coagulation Analyzer. Their expertise advice on equipment techniques and analyzing methods accelerated my research

Ngày tải lên: 11/09/2015, 10:06

... vice versa. The side effects of the anticancer drugs not only decrease effective chemotherapy, but also reduce the life of patients. Chemotherapeutic engineering represents a new challenge for ... polymers, peptides and unstable molecules requires methodologies to reduce this effect. The most preferred method to decrease steric hindrance has been to alter the synthesis approach either by ... transformed cells. These genetic abnormalities in cancer affect 2 types of genes namely Tumor suppressor genes and Oncogenes. In cancer, the oncogenes are activated and the tumor suppressor genes are inactivated.

Ngày tải lên: 16/10/2015, 15:36

... associated with neurodegenerative diseases such as Alzheimer’s diseases Parkinson’s diseases, strokes and neurotoxicity.[56-58] Because eNOS is important in regulating the blood pressure in the ... compounds could be done (i .e respective IC50), for a more conclusive comparison between the results in order to achieve the objectives completely Keywords: Nitric Oxide Synthase; Selective inhibition ... generated under these conditions does not decompose to give equal amounts of NO 2and NO3-... calmodulin-recognition sequence between the oxygenase domain and the reductase domain is the striking

Ngày tải lên: 26/11/2015, 23:06

... and were The following parameters were recorded to evaluate the effect of arginine and vitamin E supplementation on the interaction between T-2 toxin and S gallinarum: Growth rate study Body weights ... compared to the negative controls However, a significant increase in FCR of broiler birds after second week of salmonella challenge was reported by earlier workers (Ramesh et al., 2000a) The systemic ... they are reared are not optimal The stress will be further amplified, if the feed is contaminated even with low levels of immunosuppressive agents like T-2 toxin leading to lowered immunity and

Ngày tải lên: 09/01/2020, 17:15

... was performed using a 7900HT Fast Real-Time PCR System (Applied Biosystems) Predesigned and validated gene-specific probe-based Taq-Man Gene Expression Assays were used and relative gene expression ... for some genes as observed in the box plots (Fig and Table 2) Regarding the methylome, median and third quartile were zero while the average was between 0.8 and 1.8 readings per gene revealing again ... Nine genes were identified overexpressed in both basal-like breast cancer cells and BRCA1 mutated cells Amongst them, at least genes code for proteins implicated in epithelial cell migration and

Ngày tải lên: 15/01/2020, 07:00

... Prefecture Although my office was more than 400 km apart from the epicenter of the earthquake, the quake was at a level that I have never experienced before When I checked the news about the earthquake ... does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use The publisher, the ... Yet, when I announced to the registered members of the internet survey company about the survey, I was able to gather more than 8,000 respondents Hence, I realized how interested people were about

Ngày tải lên: 17/01/2020, 13:56

... Prefecture Although my office was more than 400 km apart from the epicenter of the earthquake, the quake was at a level that I have never experienced before When I checked the news about the earthquake ... does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use The publisher, the ... Yet, when I announced to the registered members of the internet survey company about the survey, I was able to gather more than 8,000 respondents Hence, I realized how interested people were about

Ngày tải lên: 20/01/2020, 13:05

... Prefecture Although my office was more than 400 km apart from the epicenter of the earthquake, the quake was at a level that I have never experienced before When I checked the news about the earthquake ... does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use The publisher, the ... Yet, when I announced to the registered members of the internet survey company about the survey, I was able to gather more than 8,000 respondents Hence, I realized how interested people were about

Ngày tải lên: 02/03/2020, 15:44

... Coumarin, Neurodegenerative disorder, Alzheimer’s disease, Acetylcholinesterase, Butyrylcholinesterase, Monoamine oxidase Introduction Alzheimer’s disease (AD) is the most common form of neurodegenerative ... [7] Razavi et  al designed and synthesized a series of 4-hydroxycoumarin derivatives [27] They screened them towards electrophorus electricus acetylcholinesterase (eelAChE) and horse serum butyrylcholinesterase ... the progression of Alzheimer’s disease [4] (AChE), APP, β-secretase, γ-secretase, monoamine oxidase (MAO) and metal chelators [5] The first line treatment that is given to AD patients is AChE

Ngày tải lên: 29/05/2020, 12:25

... Corporation, Germany) at room temperature with DMSO-d6 as a solvent Mass spectra were recorded on a TRACE 2000 spectrometer (Finnigan Corporation, America) Elemental analyses were determined on an Elementar ... natural heterocycles plays a important role in the searching for bioactive lead compounds [2, 3] As attractive nitrogenous heterocycles, tetramic acid derivatives are widely researched for some reasons ... protons were 5.39, 10.10 and 7.26  ppm in the NOESY spectrum of compound 5f (DMSO-d6), respectively The obvious NOE phenomena between ­Hj and ­Hf, and between ­Hj and ­Hk indicated that these protons

Ngày tải lên: 29/05/2020, 13:13

... the oven temperature was increased to 240 °C at a rate of 5 °C min−1 and then kept for 5 min The inlet and FID temperatures were 230 and 250  °C, respectively, and the total analysis time was 64 min ... SAS Institute, Inc.) using the general linear model procedure In addition, the least significant difference (LSD) test was conducted at the 0.05 probability level to determine the differences ... S1 and S2 Extraction and? ?analysis of? ?vitamin E Vitamin E molecules, including tocopherols and tocotrienols, were extracted by a previously reported method [17] For the purpose of vitamin E extraction,

Ngày tải lên: 29/05/2020, 13:40