visualization of mrna expression in the zebrafish embryo

... control (C) The expression of ICN protein is elevated in ICC cell lines Notch1 staining was observed in normal liver tissue; only weak staining was observed in the cell membrane and cytoplasm of a few ... up-regulated in ICC tissues and cell lines The exogenous expression of Notch1 in glioma cells increased their migratory and invasive capacity Similarly, the suppression of Notch1 expression inactivated ... Denmark) for h The degree of immunostaining was reviewed and independently scored by two observers based on the proportion of positively stained tumor cells and intensity of staining Migration

Ngày tải lên: 05/11/2020, 06:29

... annotations in the zebrafish brain atlas We refer to this latter region as Sup in our scoring of expression patterns In cluster 2, most of the TR genes are detected in the Dc which contains more cells in ... treated in parallel in the same staining experiment Tissue annotations of in situ expression data were transformed into a matrix of gene expression (0 no expression, 0.5 moderate expression, high expression) ... differences in gene expression For example, the mRNA of the bHLH TF Olig2 is expressed in two sub-regions of the proliferative zone of the telencephalon, one of which is located in the RMS and

Ngày tải lên: 01/11/2022, 09:08

... includes the named veterinary surgeons of the College of Medicine and Veterinary Medicine, the University of Edinburgh, and by the UK Home Office prior to the commencement of experimental work Funding ... be distinguished in both the wild type and mutant forebrain [22, 44], making it possible to compare the expression of Barhl2 in these regions Nestin staining for radial glial cells in the ventricular ... did the absence of Pax6 prevent the loss of Barhl2 expression from the prethalamus Absence of Pax6 did, however, increase Barhl2 expression in the thalamus and pretectum This interesting finding

Ngày tải lên: 24/11/2022, 17:49

... To investigate their functional activities in the context of the developing embryo we generated discrete germline targeted deletions (Fig 2A, Fig S2) Surprisingly, homozygous mice lacking the ... (EB) and then differentiated in N2B27 medium, 20ng/ml ActivinA and 20ng/ml EGF for a further days (B) qPCR of Eomes mRNA demonstrates a dramatic increase in expression over the course of the day ... However, when we engineered germline deletions to evaluate their functional contributions in vivo, surprisingly, only the VPE was found to influence expression in the early embryo We also exploited

Ngày tải lên: 04/12/2022, 10:32

... HIF-1α may have an important role in chondrocyte viability as a cell survival factor during the progression of osteoarthritis (OA) The expression of HIF-1α mRNA in human OA cartilage samples was ... involvement of HIF-1α in the pathogenesis of osteoarthritis Kazuo Yudoh, Hiroshi Nakamura, Kayo Masuko-Hongo, Tomohiro Kato and Kusuki Nishioka Department of Bioregulation, Institute of Medical ... online http://arthritis-research.com/content/7/4/R904 Research article Open Access Vol No Catabolic stress induces expression of hypoxia-inducible factor (HIF)-1α in articular chondrocytes: involvement

Ngày tải lên: 09/08/2014, 06:23

... binding parameters were determined using Scatchard analysis [32]. The specific binding was deter- mined by subtracting non-specific binding from the total. The binding parameters, maximal binding ... the fold change in expression. This was done by normalizing the resulting threshold cycle (CT) values of the target mRNAs to the CT values of the internal control β-actin in the same sam- ples ... counter. The non-specific binding determined showed 10% in all our experiments. Protein determination The amount of protein was measured by the method of Lowry et al., 1951 [31] using bovine serum

Ngày tải lên: 10/08/2014, 05:21

... promoter and the GFP tag, but without the Shh insert Therefore, presence of GFP in prostates indicated successful introduction of pCX-shh-IG vector and expression of Hh protein Intraprostatic injection ... whether P63+ basal/stem cells were activated under the influence of Hedgehog overexpression, we examined the pCX-shh-IG-injected prostates during the progression of PIN toward CaP to gain further ... expressed in the P63+ basal/stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells in regions of primary PIN lesions (Figure 3B; arrow in (b) indicated

Ngày tải lên: 10/08/2014, 05:21

... than other endothelial cells in the body In keeping with the theory of endothelial cell activation having a role in ANFH, coagulation-related gene expression in particular serine (or cysteine) ... development of early steroid-induced ANFH Determination of whether A2M over -expression in our study is either the result or the cause of the apoptosis found in our rats developing early ANFH following ... μl of the sample including any precipitate that may have formed was transferred into an RNeasy Mini Spin Column The column was then processed according to the RNeasy Mini kit manufacturer instruction

Ngày tải lên: 12/08/2014, 14:22

... combining in silico search for env genes within the rabbit genome, RT-PCR assays for their in vivo tran- scriptional activity in a large panel of tissues including the placenta, cloning of the ... lack of cell-cell fusion observed in vivo at the level of the placenta of the knockout embryos, resulting in impaired maternal-fetal exchanges and death of the embryos at mid-gestation [8]. Therefore, ... haematoxylin and eosin staining of a day 12 placenta section (left) with the 3 main layers of the placenta indicatedFigure 6 Structure and in situ hybridization for syncytin-Ory1 expression of day

Ngày tải lên: 12/08/2014, 23:22

... whether therapeutic hypothermia alters this inflammatory response. This study sought to examine whether hypothermia or the combination of hypothermia with anesthetic post-conditioning with sevoflurane ... mentation in mitochondria of the cerebral cortex [51]. Besides the regulation of inflammatory molecules, mild therapeutic hypothermia significantly attenuated the mRNA expression of the apoptosis-regulating ... proteins towards a reduction of active programmed cell death by increasing the hippocampal concentration of the anti-apoptotic proteins Bcl-2, and by inhibiting the ischemia-induced upregulation of

Ngày tải lên: 13/08/2014, 20:21

... glycine to L-serine prior to its potential conversion to pyruvate, was found only in late phase growth Intriguingly, the enzymes involved in biosynthesis of the amino acids arginine, lysine and ... demand for these amino acids, it is of note that the co-product of arginine synthesis is fumarate, which has the dual role of being an intermediate in both the TCA cycle as well as in gluconeogensis ... change in protein expression between proteins from the two growth conditions can be seen in Table Taking the ≥20% cut-off value, 44 proteins significantly changed in expression; at the ≥50% cutoff

Ngày tải lên: 14/08/2014, 07:21

... early in the IDC, the actual protein expression profiles of these components remain to be measured Furthermore, the expression of the proteins themselves may not be coordinated with their activation, ... decapping Lsm1 PF11_0255† Involved in decapping Lsm2 PFE1020w Involved in decapping Lsm3 PF08_0049 Involved in decapping Lsm4 PF11_0524 Involved in decapping Lsm5 PF14_0411 Involved in decapping ... study, including ribosomal proteins, proteins involved in energy metabolism, and proteins involved in rRNA processing These authors also measured a general increase in mRNA stability during the

Ngày tải lên: 14/08/2014, 07:22

... domain at different stages of differentiation, in the retina or ectopically in the spinal cord. The red box represents the 5'-untranslated region and the first nine nucleotides of the olSix3.2 ... 5c). This reca- pitulates the endogenous expression of the gene (Figure 1q). To determine the minimal region of module I involved in the control of this expression, we engineered five 5' to ... or in the hypothalamus and telencephalon (cXXIX; Figure 5g), clearly indicating that deleted elements are the main driver of olSix3.2 expression in these regions. The elements contained in the

Ngày tải lên: 14/08/2014, 07:22

... determine whether we could detect any changes in gene expression before appearance of symptoms, we examined the gene expression profile of peripheral blood in the early preclinical stages of infection ... to the process of fibrin dissolution, including those for urokinase plasminogen activator (uPA) and uPA receptor, as well as the plasminogen activator inhibitor type of the plasminogen-cleaving ... subtracting the average of the pre-infection baseline expression level from that animal, so that the values of each time point represent changes relative to the uninfected samples The genes whose expression

Ngày tải lên: 14/08/2014, 08:20

... .. expression in treatment 2 minus the expression in treatment 1; see Figure 2), the expression due to nitrogen (the expression in treatment 2 minus the expression in ... examine the reg- ulation of these funcats in all of the sub-models. This analysis enabled us to determine into which sub-models the genes from these funcats fell and to determine whether the ... from the analysis. AlignAce motifs were tested to determine whether they are over-represented in the promoters of genes whose promoters contain any of the C-elements and are in the C-only inductive

Ngày tải lên: 14/08/2014, 14:21

... distribution of its protein 3.2.3 Cos2 as a repressor in Hh signaling pathway affecting myotome of zebrafish embryo 3.2.4 Cos2 inactivation affects the developing neural tube 3.2.5 The activity of zebrafish ... microtubules in mammalian cells 55 3.3 3.4 57 Loss of Cos2 function results in de-repression of the Hh pathway in the myotome of the zebrafish embryo Inactivation of Cos2 function induces ectopic ... through to the vertebrates However, amongst them, the kinesin-like protein Costal2 (Cos2), which plays an important role in controlling the transcriptional activity of the Hh pathway, remained elusive...

Ngày tải lên: 10/09/2015, 15:49

... kinesin-like protein of 1363 amino acids was assembled Like Drosophila Cos2 and other members of the kinesin heavy chain (KHC) superfamily of proteins, the N-terminal and C-terminal regions of ... has a minimal role in the Drosophila Hh pathway, vertebrate Su(fu) behaves otherwise Morpholino knockdown against the initiation codon of Su(fu) in zebrafish resulted in a gain of Hh signaling as ... When staining reaction reached the right level of staining, staining solution was washed off with PBT and the stained embryos were stored in Fish Fix 2.5.2 Fluorescent in situ hybridization The protocol...

Ngày tải lên: 10/09/2015, 15:49

... (Dsh) The amino-terminal extracellular Wnt binding domain and the 18 19 carboxy-terminal PDZ-binding domain of the Fz protein are known to be essential for Fz function Deletion of the PDZ-binding ... finally folds into a tube, what was initially the margin of the neural plate now becomes the most dorsal portion of the neural tube (the roof plate) and the epidermis from both halves of the embryo ... incorporation into the posterior structures of the embryo This result highlights the early commitment of cell fate during the blastula period, and links the positions of cells in the gastrula to their...

Ngày tải lên: 16/09/2015, 17:12

... restricted feeding restores the rhythms of expression of the clock protein, Period2, in the oval nucleus of the bed nucleus of the stria terminalis and central nucleus of the amygdala in adrenalectomized ... either side of the blue line Therefore, in the actogram-style plots of the same data, the dark sections (indicating higher temperature) should also be symmetrical on either side of the blue line ... autoradiographs of Bmal1 expression in one or more whole coronal sections of the brain, showing expression in the target structure and no expression outside of this structure in brain regions that...

Ngày tải lên: 10/08/2014, 09:20

... http://respiratory-research.com/content/11/1/150 The data demonstrate there is expression of genes for a complete GABAergic system in the airway epithelium Interestingly, the expression of GAD67 was markedly modified by smoking, with increased ... protein concentration To assess the Western analyses quantitatively, the film was digitally imaged, maintaining exposure within the linear range of detection The contrast was inverted, the pixel intensity ... that incubation of rhesus macaque bronchial epithelial cells with nicotine for 48 hr significantly increased mucin mRNA levels Interestingly, the effect of nicotine was blocked both by the nicotinic...

Ngày tải lên: 12/08/2014, 11:22