visualization of mrna expression in the zebrafish embryo
Hedgehog signaling in the zebrafish embryo role of kif7 and DZIP1 1
Ngày tải lên :
10/09/2015, 15:49
... distribution of its protein 3.2.3 Cos2 as a repressor in Hh signaling pathway affecting myotome of zebrafish embryo 3.2.4 Cos2 inactivation affects the developing neural tube 3.2.5 The activity of zebrafish ... microtubules in mammalian cells 55 3.3 3.4 57 Loss of Cos2 function results in de-repression of the Hh pathway in the myotome of the zebrafish embryo Inactivation of Cos2 function induces ectopic ... through to the vertebrates However, amongst them, the kinesin-like protein Costal2 (Cos2), which plays an important role in controlling the transcriptional activity of the Hh pathway, remained elusive...
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Hedgehog signaling in the zebrafish embryo role of kif7 and DZIP1 2
Ngày tải lên :
10/09/2015, 15:49
... kinesin-like protein of 1363 amino acids was assembled Like Drosophila Cos2 and other members of the kinesin heavy chain (KHC) superfamily of proteins, the N-terminal and C-terminal regions of ... has a minimal role in the Drosophila Hh pathway, vertebrate Su(fu) behaves otherwise Morpholino knockdown against the initiation codon of Su(fu) in zebrafish resulted in a gain of Hh signaling as ... When staining reaction reached the right level of staining, staining solution was washed off with PBT and the stained embryos were stored in Fish Fix 2.5.2 Fluorescent in situ hybridization The protocol...
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Molecular mechanisms involved in the specification of embryonic ectoderm in the zebrafish embryo
Ngày tải lên :
16/09/2015, 17:12
... (Dsh) The amino-terminal extracellular Wnt binding domain and the 18 19 carboxy-terminal PDZ-binding domain of the Fz protein are known to be essential for Fz function Deletion of the PDZ-binding ... finally folds into a tube, what was initially the margin of the neural plate now becomes the most dorsal portion of the neural tube (the roof plate) and the epidermis from both halves of the embryo ... incorporation into the posterior structures of the embryo This result highlights the early commitment of cell fate during the blastula period, and links the positions of cells in the gastrula to their...
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Báo cáo khoa học: " On the visualization of universal degeneracy in the IMRT problem" ppt
Ngày tải lên :
09/08/2014, 10:21
... However, this does not mean imum, then that the minimum is attained in a single point In the case of degeneracy, as commonly present in the IMRT setting, the set of minimizers is a sizeable, high ... be the set of all beamlets of an arrangement of intensity modulated beams, or the set of all arclets of radiosurgery arcs The basis set defines the parameter space of the optimisation as the ... In ∗ ∗ a convex setting, it is possible to go from D1 to D2 along a straight line without leaving the solution space, but the gradient of F in the direction of ηΔ has to increase when ∗ ∗ going...
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Báo cáo y học: "Standards of evidence in chronobiology: critical review of a report that restoration of Bmal1 expression in the dorsomedial hypothalamus is sufficient to restore circadian food anticipatory rhythms in Bmal1-/- mice" pot
Ngày tải lên :
10/08/2014, 09:20
... restricted feeding restores the rhythms of expression of the clock protein, Period2, in the oval nucleus of the bed nucleus of the stria terminalis and central nucleus of the amygdala in adrenalectomized ... either side of the blue line Therefore, in the actogram-style plots of the same data, the dark sections (indicating higher temperature) should also be symmetrical on either side of the blue line ... autoradiographs of Bmal1 expression in one or more whole coronal sections of the brain, showing expression in the target structure and no expression outside of this structure in brain regions that...
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Báo cáo y học: " Smoking-mediated up-regulation of GAD67 expression in the human airway epithelium" doc
Ngày tải lên :
12/08/2014, 11:22
... http://respiratory-research.com/content/11/1/150 The data demonstrate there is expression of genes for a complete GABAergic system in the airway epithelium Interestingly, the expression of GAD67 was markedly modified by smoking, with increased ... protein concentration To assess the Western analyses quantitatively, the film was digitally imaged, maintaining exposure within the linear range of detection The contrast was inverted, the pixel intensity ... that incubation of rhesus macaque bronchial epithelial cells with nicotine for 48 hr significantly increased mucin mRNA levels Interestingly, the effect of nicotine was blocked both by the nicotinic...
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Báo cáo y học: "Mouse maps of gene expression in the brain" pot
Ngày tải lên :
14/08/2014, 07:21
... consistent, indicating that the pattern is due to the promoter elements of the gene of interest rather than the genomic insertion point The anatomical annotators of the GENSAT data have embraced the ... distributed through the Mutant Mouse Research Resource Centers [8,9] In addition, for some of the most interesting expression patterns, the project is developing lines where the BAC containing the gene ... sites, resulting in a recombinant strain of mouse that eliminates a gene’s expression only in the cells that express the Cre These lines will be a valuable resource for investigators looking to understand...
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Báo cáo y học: "Cell-specific microarray profiling experiments reveal a comprehensive picture of gene expression in the C. elegans nervous system" ppsx
Ngày tải lên :
14/08/2014, 07:22
... observed in nine embryonically derived DA motor neurons at this stage (unc-4 is strongly expressed in the DAs in the embryo and in L1 larvae.) To increase the sensitivity of the mRNA- tagging method ... number of genes in a given neuron and are, therefore, incomplete To test the applicability of the mRNA- tagging strategy for obtaining a comprehensive gene expression profile of a specific subset of ... transport in neurons [68] Other members of this interacting complex, MKK-4 (MAP kinase kinase) and JNK-1 (Jun kinase) are also required for maintaining normal synaptic structure [69,70] These findings...
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Báo cáo y học: "The LeFE algorithm: embracing the complexity of gene expression in the interpretation of microarray data" pps
Ngày tải lên :
14/08/2014, 08:20
... value of the training samples in the case of continuous variables The aggregate forest's output is then determined by averaging the regression values of the trees or using a weighted voting process ... basis, the random forest determines the importance score of each gene (variable) in the multivariate model The distribution of importance scores of the genes in the category is then compared with the ... applications of LeFE, to gene expression in three breast cancer classes and to in vitro gefitinib sensitivity (see Results), provide further proofs of principle The findings highlight the distinctions...
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Báo cáo y học: "The LeFE algorithm: embracing the complexity of gene expression in the interpretation of microarray data" potx
Ngày tải lên :
14/08/2014, 08:20
... SLA2/Huntingtin-interacting protein Hip1 Y66H1B.3 462 784 Actin-binding cytoskeleton protein, filamin Y66H1B.2 119 902 Actin-binding cytoskeleton protein, filamin 766 20 Actin-binding protein Coronin, contains WD40 ... Actin regulatory proteins (gelsolin/villin family) Y71G12B.11 122 Talin W03F11.6 afd-1 - 393 Actin filament-binding protein Afadin F08A8.6 tag-138 - 474 Actin-binding protein SLA2/Huntingtin-interacting ... encoding proteosome subunits show elevated expression in the M24 dataset whereas none of these transcripts are enriched in the M0 profile This finding could be indicative of the general lack of innervation...
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Báo cáo y học: " Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo" pptx
Ngày tải lên :
14/08/2014, 08:20
... shown) The thioredoxin-like mRNA is also expressed in selected areas of the brain These regions show also increased levels of expression in response to Cd (data not shown) In summary, these in situ ... number of neurons in the brain In in situ hybridization experiments, strong elevation of thioredoxin-like mRNA levels in response to Cd and MeHg was also noticed in the hair cells of the lateral line ... studies exist [1,21,22] Dioxin (TCDD) impairs fin regeneration in adult zebrafish, and expression profiling revealed TCDD-induced changes in the expression of genes involved in extracellular matrix...
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Báo cáo y học: "Microarray analysis of microRNA expression in the developing mammalian brain" ppt
Ngày tải lên :
14/08/2014, 14:21
... interactions Figure Profile of microRNA expression in the developing mouse brain Profile of microRNA expression in the developing mouse brain (a) Relative expression levels for the 66 microRNAs that ... by the RNAse Drosha in the nucleus, cleaving the RNA at the base of the stem-loop [25] This cut defines one end of the microRNA The precursor microRNA is then exported by Ran-GTP and Exportin-5 ... microarray to monitor expression of microRNAs during mouse brain development We determined the temporal expression pattern of 138 microRNAs in the mouse brain and found that the levels of 66 microRNAs...
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Báo cáo y học: "Global analysis of mRNA stability in the archaeon Sulfolobus" pps
Ngày tải lên :
14/08/2014, 17:22
... out of 7) As a final example, within the group of predicted nucleic acid-binding proteins containing a PIN domain, 11 out of 12 transcripts displayed half-lives above median In S solfataricus, these ... transcripts in one-, two- and three-gene runs; 50% of the genes are included in the boxes, and 80% between the whiskers; the line within the box represents the median half-life (c) Scatter plot of mRNA ... constitute the 'toxin' in the 'toxin-antitoxin' loci of vapBC-type that recently were found to be abundant in archaeal species [21] The cellular target(s) of the VapC toxins is not yet clear, but in...
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Báo cáo khoa học: "Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis" pdf
Ngày tải lên :
07/08/2014, 17:22
... were found to express nestin and/or vimentin in rats with EAE This finding is in part Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental ... region showed intense nestin immunostaining At the recovery stage of EAE (day 21 PI), the nestin immunostaining pattern was similar to the pattern at the peak stage of EAE, with fewer nestin-positive ... Tae-kyun Shin, Yong-duk Lee and Ki-bum Sim nestin, vimentin and GFAP in EAE lesions in the spinal cords of Lewis rats to evaluate the activation capacity of brain cells during autoimmune inflammation...
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Báo cáo khoa học: EcR expression in the prothoracicotropic hormoneproducing neurosecretory cells of the Bombyx mori brain ppt
Ngày tải lên :
16/03/2014, 13:20
... unique expression profile indicates the importance of EcR isoform expression in TCCs and OLCs during larval-pupal metamorphosis, although the roles of the individual isoforms in these cells remain ... arrows in (D) and (E) indicate the EcR-A- and EcR-B1-producing cells in the tritocerebral region of the pupal brain, respectively Red arrows indicate the EcR-Aor EcR-B1-producing cells in the subesophagal ... reorganization of the brain 20E induces differentiation of the optic lobe and adult eye in Manduca [5,36], while EcR is expressed during the puparium stage in the optic lobe of Drosophila [16] In the present...
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Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc
Ngày tải lên :
17/03/2014, 17:20
... protein and the mRNA were faint Effects of inhibitors of cGCS and GR on primary cultured testicular cells To investigate the contribution of de novo synthesis and the recycling of GSH to the intracellular ... protein metabolism in these cells Histone is rich in the basic amino acids, lysine and arginine, but contains no cysteine Protamine, which is replaced for hitstone via transit proteins during ... level of GR in the epithelia of the epididymis (Fig 1) As the epididymis is rich in sulfoxidase, the oxidation of sulfhydryls in the sperm head and tail would be mediated by this enzyme in a specific...
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Báo cáo khoa học: Expression, localization and potential physiological significance of alcohol dehydrogenase in the gastrointestinal tract pdf
Ngày tải lên :
23/03/2014, 17:22
... found in the epithelial cells lining the surface of gastric pits of the gastric body (Fig 3F) Detection by IHC confirmed expression of ADH4 in the mucus-secreting cells of pylorus (Fig 3G) Therefore, ... immunostaining was detected in the stratified squamous epithelium (Fig 2I) Interestingly, a strong ADH4 protein signal was observed in the keratinized layer of epithelium, where the ADH4 mRNA was ... gradually appeared in the mucussecreting epithelium as well (cf Fig 3B,C,D) In contrast, ADH4 mRNA was detected in the mucus-secreting cells, in some of the inner cell layers, and in muscularis mucosae...
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báo cáo hóa học:" Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs" pot
Ngày tải lên :
20/06/2014, 00:20
... and plateau, and cut to match the surface area of the condyle The areas of India ink staining were outlined using a permanent marker Tracings of the India ink-stained tibial and femoral condyles ... assessments The lamina splendens was not disrupted in any location based on lack of India ink staining, indicat- ing that AC surface integrity was maintained for two weeks in the dogs in this study ... influence the "point of no return" in the progression of OA are chondrocyte viability and phenotype During the development of OA, there is often a focal increase in cell death[15-18] Since it is theorized...
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Báo cáo khoa học: "Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages" pot
Ngày tải lên :
07/08/2014, 15:20
... level of each cytokine gene was normalized against that of the cyclophilin A gene The final expression of each cytokine gene was determined by subtracting the expression level of a cytokine in control ... 48, and 72 h of incubation To normalize the expression levels of each cytokine, the ratios of each cytokine/cyclophilin A were calculated, and the ratios of cytokine/cyclophilin A in the control ... stimulate expression of both cytokines in vivo Taken together, the mRNA expression profiles of IL-6, IL-12, and other cytokines examined in this study would be useful references for the diagnosis of...
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Báo cáo khoa học: "Changes in orexin-A and neuropeptide Y expression in the hypothalamus of the fasted and high-fat diet fed rats" doc
Ngày tải lên :
07/08/2014, 18:20
... results and those of Mondal et al [26] in terms of the number and the contents of the OXA-IR neurons, the increase-tendency in the number of the OXAIR neurons in the LHA of the fasting rats was consistent ... starvation for survival The increase of NPY expression in the ARC may be stimulated by the decrease of leptin in blood at 84 h of fasting, but not on the OXA The expression of OXA and NPY may rise ... study is to investigate the effect of the high-fat diet on the expression of OXA and NPY in the hypothalamus of the induced SD obese rats as well as the effect of the fasting on normal SD rats...
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