viruses infection of mice and tissue collection

... the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses Table 1: ... evidence for the presence of both RV1 and RV2 macaque rhadinoviruses, RFHVMn and MneRV2, respectively, in RF tumor and spleen tissue of this animal [5] Fresh frozen RF tumor tissue from Macaca mulatta ... interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques Only two healthy macaques had detectable RRV DNA with levels of 320 and 880 genomes...

Ngày tải lên: 18/06/2014, 22:20

... the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses Table 1: ... evidence for the presence of both RV1 and RV2 macaque rhadinoviruses, RFHVMn and MneRV2, respectively, in RF tumor and spleen tissue of this animal [5] Fresh frozen RF tumor tissue from Macaca mulatta ... interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques Only two healthy macaques had detectable RRV DNA with levels of 320 and 880 genomes...

Ngày tải lên: 20/06/2014, 04:20

... IL-17A (10 and 20 ng/ml) and 4.4 fold by TNF-α from basal Page of 12 (page number not for citation purposes) of 9202.64 ± 8806.31 ng/mg of tissue to 16,711.80 ± 15,535.07 ng/mg of tissue (P < ... cohort and participated in study design and data analysis JMcC, MC and OS performed some experiments BB and OF participated in study design and data analysis UF and DV conceived the study and developed ... therapy Materials and methods Synovial tissue, serum and synovial fluids All patients fulfilled the 1987 American College of Rheumatology criteria for a diagnosis of RA [30] and had actively inflamed...

Ngày tải lên: 09/08/2014, 14:22

... One of the hallmarks of tumor development and maintenance is defiance of tumor cells to execute death signals (Thompson CB, 1995) Thus, a combination of increased proliferation and lack of cell ... driven by chemical gradients of Na+ and H+ and not require energy, the increase in transport activity of NHE1 is often associated with phosphorylation of a number of serine residues within the ... transfection mixture, µg of DNA, (5 µg of the required plasmid and µg of the pCMV β-gal plasmid) was diluted in sterile ddH2O in a microfuge tube, followed by the addition of 12.4 µl of 2.5 M CaCl2 in...

Ngày tải lên: 16/09/2015, 08:31

... inefficient and of inadequate clinical value to permit its use The widespread availability and applicability of safe and effective human germline gene therapy would require the development of gene ... and maintenance of transgenes Several of the studies report the subsequent generation of viable progeny animals, the cells of which contain transgene DNA sequences While a minority of studies have ... advantages of simplicity and cost-effectiveness, in contrast with more established methods of transgenesis such as pronuclear micrinjection However, despite the above successes and regardless of its...

Ngày tải lên: 03/11/2012, 10:01

... with tourist We ordered a bottle of orange juice, drank and wait for a chance to access tourists And we got it Most of tourists here were very cheerful, very humor and very easy to get an open talk ... Therefore, they spend more time and money for travelling, and for relaxing in some Southern Eastern Asian countries: Thailand, Singapore, Laos, Cambodia, and Vietnam And the reason why the north ... interview one to two travelers Most of them seemed too closed, to be friendly and warmhearted, and welcome us They told us everything they did in Vietnam and they thought about Vietnam in their...

Ngày tải lên: 25/04/2013, 15:42

Ngày tải lên: 20/07/2013, 01:27

... HAc RESULTS AND DISCUSSIONS Figure shows a typical CO2/pH profile of sodium acetate addition in the CO2 profile; the rising time between the left foot of the slope and right edge of the peak ... maintained within the range of 50 to 60 ºC and volatile solid destruction averaged about 30% at days of HRT Digested sludge was centrifuged for 10 minutes at 3,000 rpm and supernatant was withdrawn ... test, a certain volume of sodium acetate (1,000 mg/L as HAc) was added and the carbon dioxide profiles were monitored until the reaction completed Unknown concentrations of sodium acetate were...

Ngày tải lên: 05/09/2013, 08:40

... Variations of these fluxes give the shape of the backelectromotive forces e,, e,, ,ec Using the extension of the Park's transformation, @Ira , @Irp and the variations of the angle p and of versus ... is a function of We can verify t a , in the sinusoidal case, ht p=O p depends only of the value of the variations of rotor fluxes, witch can be easily known through a measuring of back electromotiveforces, ... inkrval of electrical angle where Wra is near of its maximal value @', supplied with a negative voltage -E during the 120" interval of eltvtrical angle where @Im is near of its minimal value -@I, and...

Ngày tải lên: 03/01/2014, 19:50

... end of an a-helical region of the HFE a1 domain (V100 and W103A) as being of particular importance for TfR1 interactions [16,17,20] The effect of HFE binding to TfR1 is to lower the affinity of ... sites of HFE N-glycosylation and examine the importance of glycosylation on parameters of protein localization and function Results Tunicamycin treatment results in a reticular pattern of HFE ... mutant forms of HFE and incubated the cells in the presence and absence of tunicamycin Drug treatment resulted in the accumulation of an unglycosylated lower molecular mass form of the wild-type...

Ngày tải lên: 06/03/2014, 22:21

... vector (ZBP-89i) increased expression of p16 and induced cell senescence Moreover, overexpression of HDAC3 and HDAC4 resulted in repression of p16 expression, and HDAC3 was recruited to the p16 ... expression of the ZBP-89 vector (Fig 1A), and the efficiency of inhibition of ZBP-89 expression by ZBP89i (Fig 1B) The transfected NCI-H460 cells were then lysed and assayed for the activity of senescenceassociated ... HDAC3 and HDAC4 were recruited to the p16 promoter by ZBP-89, we examined the binding of HDAC3 and HDAC4 in different regions of the p16 promoter upon knockdown of the endogenous ZBP-89, and the...

Ngày tải lên: 07/03/2014, 02:20

... Parkinson’s and Creutzfeldt–Jakob diseases) or a variety of peripheral tissues and organs (systemic amyloidoses and type II diabetes) [1] Since 1998, a growing number of peptides and proteins ... avoid the appearance of misfolded proteins and their initial aggregates in tissue Notwithstanding the validity of such an approach, better knowledge of the biochemical basis of cell vulnerability ... aggregates and cells, and the response of cells to the presence of aggregates [14,31] According to our previous data, the differing extent of alterations in ROS and free Ca2+ in IMR90 and Hend...

Ngày tải lên: 16/03/2014, 13:20

... regulation of a variety of cellular processes, including pericellular proteolysis, cell–cell or cell–ECM adhesion, and regulation of membrane architecture [13–16] One of the important functions of extracellular ... Immunoblotting of natural annexin II purified from CaR-1 cells under nonreducing ()) and reducing (+) conditions 2ME, 2-mercaptoethanol The bands at 72 and 36 kDa correspond to dimeric and monomeric forms of ... immunoblotting of the culture supernatant showed two close bands at 36 and 37 kDa under nonreducing conditions, but a single band of 36 kDa under reducing conditions The 37 kDa band appeared to...

Ngày tải lên: 17/03/2014, 17:20

... Protein and total RNA were extracted from various EOC cell lines, HEK293 and LNCaP cell lines (A, B) Expression levels of protein and mRNA, and levels of p53, ANX4 and the known p53 targets, p21 and ... cell lines and tissue compared to a mucinous adenocarcinoma-type cell line and tissue Subsequently, Zhu et al [14] compared proteomic patterns in 16 CCA and eight serous tissue samples, and also ... Protein and RNA were extracted from two CCA (OVTOKO and OVISE) cells lines and one non-CCA (MCAS) cell line, and then ANX4 protein (A) and mRNA (B) levels were compared by western blotting and real-time...

Ngày tải lên: 22/03/2014, 16:20

... scale of the cancer problem and the impact it has on the lives of hundreds of thousands of people every year We have made huge steps forward in improving survival and in preventing thousands of ... from 46% in the 1970s Our work has been at the heart of this progress and has saved the lives of hundreds of thousands of people in the UK and many millions more around the world • We were the ... services Our brand and reputation The Cancer Research UK brand enjoys high levels of awareness, emotional support and trust The advances we make set us apart and deliver a message of hope But our...

Ngày tải lên: 22/03/2014, 16:21

... SP1 and AP-2a regulate KCTD10 R Liu et al A B Fig Genomic structure of human and mouse KCTD10 genes, and the 5¢-flanking region sequence of human KCTD10 (A) Genomic organization of human and mouse ... FEBS R Liu et al SP1 and AP-2a regulate KCTD10 Roles of Sp1 and AP-2a in the regulation of the promoter activity and the expression of human KCTD10 Fig Transcription start site of human KCTD10 Antisense ... Alignment of the 5¢-flanking sequences upstream of the start codon of human and mouse KCTD10 B Fig In vivo ChIP assays of Sp1.1 and AP-2.2 binding sites in the proximal promoter region of human...

Ngày tải lên: 30/03/2014, 02:20

... CPT, the rate of relaxation of LdTOPIL-fus-S was greater than that of LdTOPIL-fus-D(1–210)S (compare lanes and of Fig 4B with lanes and of Fig 4C) In the presence of CPT, it can be seen that ... doublestranded DNA in the presence of vanadate The vanadate forms covalent linkages between the catalytic Tyr222 residue of the small subunit (LdTOPIS) and the nicked ends of the scissile DNA strand, ... reconstituted LdTOPIL and LdTOPIS were used both in the absence and the presence of Mg2+-containing buffer Effect of CPT on the relaxation activity and equilibrium cleavage activity of fused topoisomerase...

Ngày tải lên: 30/03/2014, 10:20

... 2.27 The set of p-adic integers Zp is a subring of Qp Every element of Zp is the limit of a sequence of (non-negative) integers and conversely, every Cauchy sequence in Q consisting of integers ... reminiscent of the decimal expansion of a real number but with possibly infinitely many positive powers of p This is the (standard ) p-adic expansion of α ∈ Zp and the αn are known as the (standard ... the set of units in Z/n and {zero divisors} the set of zero divisors Furthermore, (a) z is a zero divisor if and only if gcd(z, n) > 1; (b) u is a unit if and only if gcd(u, n) = Proof If h =...

Ngày tải lên: 31/03/2014, 15:19

Ngày tải lên: 18/04/2014, 08:21