virus like particle vaccines advantages and challenges

... VP1 assemble into virus- like particles (VLPs) when expressed in insect cells infected with a recombinant baculovirus [4] VP1 folds into two major domains termed the shell (S) and protruding (P) ... prevent virus spread and contain epidemics Results VL and VH domains of mAb 54.6 and design of scFv54.6 Anti-rNV mAb 54.6 recognizes non-denatured VP1, inhibits VLP-mediated hemagglutination, and ... M-MLV RT (Invitrogen) and 3' oligonucleotides MVK-R and MVH-R (Table 1) for first-strand synthesis of cDNA corresponding to the variable regions of the light and heavy chains, VL and VH, respectively...

Ngày tải lên: 20/06/2014, 01:20

... Hình 1.3 Sơ đồ minh họa cấu trúc hạt virus tự nhiên (native virus) hạt giả virus (virus – like particle) VLP gọi hạt giả virus hình thái cấu trúc tương tự virus tự nhiên Phía ngồi lớp vỏ chứa ... phóng virus, ức chế lan nhiễm virus 1.2 Virus cúm A/H5N1 gây bệnh gia cầm 1.2.1 Đặc điểm cấu tạo Virus cúm H5N1 thuộc type A, họ Orthomyxoviridae Họ gồm nhóm virus: Nhóm virus cúm A, nhóm virus ... nhóm Thogotovirus Các nhóm virus khác kháng ngun bề mặt capsid, virus cúm A B Hemagglutinin (HA), virus cúm C Hemagglutinin Esterase Fusion (HEF), Thogotovirus Glycoprotein (GP) Nhóm virus cúm...

Ngày tải lên: 07/07/2014, 19:31

... ACCEPTED MANUSCRIPT Advantages and challenges of the spray-drying technology for the PT production of pure drug particles and drug-loaded polymeric carriers SC RI Alejandro Sosnik1* and Katia P Seremeta2,3,4 ... production of pure drug particles and drug-loaded polymeric particles and discusses the potential of this technique and the more advanced equipment to pave the way toward reproducible and scalable processes ... production of submicron particles with high yield, even for small sample amounts, have been introduced into the market This review describes the most outstanding advantages and challenges of the spray-drying...

Ngày tải lên: 02/09/2015, 13:31

... Incorporation of fowl plague virus hemagglutinin into murine leukemia virus particles and analysis of the infectivity of the pseudotyped retroviruses J Virol 1998, 72:5313-5317 Sandrin V, Boson B, Salmon ... influenza A virus H1 and H2 strains J Virol 1993, 67:2552-2558 Yao Q, Kuhlmann FM, Eller R, Compans RW, Chen C: Production and characterization of simian human immunodeficiency virus- like particles ... influenza virus RNAs are isolated, could therefore provide a useful method to obtain in a timely manner a set of efficient immunological reagents such as sera, antibodies and influenza virus- like particles...

Ngày tải lên: 20/06/2014, 01:20

... was purified and identified by SDS-PAGE (A) and Western blot (B) Lane M: molecular weight marker; Lane VLPs: JCV virus- like particles The rabbit antibody reactive to the JC virus- like particle is ... contributions MIC and YFH contributed equally to this work MIC and YFH interpreted the data and drafted the manuscript MIC, MW, DC and JTC designed and performed the RNAi and JC virus experiments MZ and GJT ... that the recombinant JC virus major capsid protein, VP1, is able to self-assemble to form a virus- like particle (VLP) when expressed in the baculovirus [4], E coli [14] and yeast [12] In addition,...

Ngày tải lên: 10/08/2014, 05:21

... Figure capsids virus- like particles under reducing (+DTT) and under minutes at the indicated temperatures fraction of The stability of PP7 virus- like particles under reducing (+DTT) and under non-reducing ... stability of 2PP7 virus- like particles Denaturation of 2PP7 virus- like particles after two minutes at a variety of temperatures is shown in Figure With disulfide bonds intact the particle was stable ... PP7 and 2PP7 virus- like particles confirmed that the 2PP7 capsid was cross-linked into a covalent structure of high order (Figure 4) The unreduced PP7 particle yields two closely spaced main bands...

Ngày tải lên: 11/08/2014, 00:22

... G, Johnson DA: Dose-ranging studies of the safety and immunogenicity of human papillomavirus Type 11 and Type 16 virus- like particle candidate vaccines in young healthy women Vaccine 2004, 22:2943-2952 ... epitopes on virus- like particles for human papillomavirus types 6, 11, 16, and 18 by a multiplexed Luminex assay Clin Diagn Lab Immunol 2003, 10:108-115 O'Connell MA, Belanger BA, Haaland PD: The ... human serum; VLP, virus- like particles; HPV, human papillomavirus; GMT, geometric mean titers; Ig, immunoglobulin; Ab, antibody 10 Competing interests W Ruiz, W McClements, K Jansen and M Esser were...

Ngày tải lên: 11/08/2014, 10:23

... involves the bypass of a drug-sensitive assembly and budding pathway by the virus or virus- like particle progeny The results of our study suggested that DSB and other betulinic acid derivatives could ... anti-Gag and anti-Vif rabbit primary antibodies and 35S-labelled secondary anti-rabbit IgG antibody After autoradiography of the blots, bands of Pr55Gag and Vif proteins were excised and their ... indicated on top of panels (a) and (b), and on the x-axis of panel (c) Cells were harvested at 48 h pi, and whole cell lysates (WCL) and extracellular VLP analyzed by SDS-PAGE and immunoblotting, using...

Ngày tải lên: 12/08/2014, 04:21

... areas in highlands and mountains aiming at satisfying local water demand and reducing poverty; • Program for Large Multipurpose Water Systems with big reservoirs (V=0.3÷1.5 billion m3 ) and high dams ... centuries and also directly protects the delta from floods Central Vietnam stretches from the North ( parallel 20o) to the South ( parallel 11o) and is composed of two subregions: the East Coastland and ... where there are sandy islands, large salty ponds, typhoonal and monsoon regime Ecosystem Ecosystem, which changes in basin from the river source to the estuarial zone during and after dam construction,...

Ngày tải lên: 01/04/2013, 22:47

... PSTN signaling and call control infrastructures, (c) service and feature extraction and insertion servers and/ or GWs for delivering the caller’s name and number, voice message, and so on to an ... (www.ansi.org) and 136 CONCLUSIONS AND CHALLENGES ITU-T, work groups of IETF, MSF, and the International Softswitch Consortium (ISC), IEEE (standards.ieee.org), Cable Labs (www.cablelabs.com/ projects/), and ... flexibility, and widespread availability of the IP-based network and services are fueling the unification of distributed computing and communications (datacom and telecom) infrastructures, and their...

Ngày tải lên: 30/09/2013, 07:20

... to getthere and back Big stores are also quite dangerous for all small shops owned by private people And those shops may be the only source of living of then To conclude I would like to express ... other hand shopping in supermarkets can be annoying beckause there is a big choice which can cause confusion People often buy unnecessary things beckause they seeing to have a good price and you ... Attractive packing and displays versuade people to buy things they not actually need For many of us the major disadvantage of shopping in supermarkets id that it is time consuming and it takes a...

Ngày tải lên: 23/10/2013, 02:15

Ngày tải lên: 01/11/2013, 14:49

... fewer and cheaper resources The situation is however more complex in the case of CDN since higher bandwidth and lower bandwidth cost also have as a side effect that customers develop more and more ... such as streaming and ondemand Important research questions are, e.g., scalability, overlay traffic measurements and modeling, data search and retrieval, load balancing, churn handling, QoS provisioning ... between the origin server and the surrogate server and the other between the surrogate server and the client On top of that, questions related to QoS, content multicasting and multipath routing heavily...

Ngày tải lên: 09/12/2013, 21:15

... productivity and efficiency gains (Amit and Zott, 2001; Lucking-Reiley and Spulbur, 2001; Wigand and Benjamin, 1995) E-commerce also is expected to facilitate entry into new markets and the extension ... S.K and Gupta, J.N.D (2001) E-Commerce Opportunities and Challenges In M Singh & T Thompson (Eds.), E-Commerce Diffusion: Strategies and Challenges Australia: Heidelberg Press Sharma, S K and ... extension of existing markets (Garicano and Kaplan, 2001), and greater integration of systems with suppliers and customers (OECD, 1999; Timmers, 1999; Wigand and Benjamin, 1995) As e-commerce continues...

Ngày tải lên: 10/12/2013, 11:16

... inefficiencies and bottlenecks and enhancing workflow using automation and other information technologies, can add additional cost savings as well as faster cycle times and other business benefits Challenges ... long-term costs such as unemployment insurance and employee benefits, and implementing the software development and quality assurance processes and practices of the offshore suppliers as a way ... management needs to thoroughly understand the challenges to successful offshoring Major risks and issues include differences in language, culture, politics, regulations, and work practices—all of which...

Ngày tải lên: 20/01/2014, 07:20

... convenient and enjoyable method I thing think that is has the there are advantages and disadvantages when who of traveling by airplane.( theo viết However, there are advantages and disadvantages ... Internet has great potential and lot to offer… however, like every single innovation in science and technology, internet has its own advantages and disadvantages Advantages Communication: The ... from government law and services, trade fairs and conferences, market information, new ideas and technical support, the list is end less Advantages and disadvantages Students and children are among...

Ngày tải lên: 26/01/2014, 01:20

... Switzerland, United Kingdom, Holland, USA and Japan) Imports were directed mainly to Germany, the Netherlands and Switzerland German and Dutch traders imported mainly spices, fruits, tea, and nuts ... mature organic markets like Austria and Denmark show poor growth rates (Hamm et al 2002) Switzerland and Denmark have the highest consumption per capita, whereas France, Holland and Italy have low ... manufacturers and exporters Large and medium-sized processors Details Travel and inspection Report preparation Certification Travel and inspection Report preparation Certification Travel and inspection...

Ngày tải lên: 14/02/2014, 03:20

... (5¢-CTTCCTCCGCTTCCATGA-3¢) and QaCgClp1 (5¢-CCATGAAGTCCGCGAATC-3¢); and QsCgClp2 (5¢-GCATAGCGATGTGGACGA-3¢) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢) The abbreviations ‘Qs’ and ‘Qa’ refer, respectively, to sense and ... edge and postspawning gonads Metamorphosis represents the ultimate stage of oyster development, and is characterized by the degeneration of larval tissues, such as the velum and the foot, and ... Cg-Clp1 (and most likely Cg-Clp2) is known to bind tightly and specifically to chitin [5] strongly supports a role of this lectin in the immune response to chitinous pathogens, such as fungi and nematodes,...

Ngày tải lên: 19/02/2014, 00:20

... observations and lessons learned from the evaluation, and suggested some possible research directions to address these challenges which may be helpful for current and new participants, or IE and QA ... Daniel Moder Henry was born Monday in Los Angeles and weighed 8? lbs Roberts, 39, and husband Danny Moder, 38, are already parents to twins Hazel and Phinnaeus who were born in November 2006.” the ... (source, anchors, redirects and disambiguation) to extend the KB and compute entity cooccurrence estimates Many other teams including CUNY and Siel used redirect pages and disambiguation pages for...

Ngày tải lên: 20/02/2014, 04:20

... several minutes and can be distributed over long distances, (e.g foot-andmouth disease (FMD) virus more than 50 km, and staphylococcae up to 500 m) This paper describes the complex nature and composition ... intensification, future, climate 47 Do old and new forms of poultry go together? Jan Slingenbergh1 and Marius Gilbert2 Animal Production and Health Division, Food and Agriculture Organization of the ... other hand, may be more capable of resisting parasites and infectious disease agents, but it should be noted that there are a growing number of exceptions, including HPAI and other, mainly RNA, viruses...

Ngày tải lên: 21/02/2014, 01:20