virus induced cell surface antigens

... for infectious virus production and virus- induced cell fusion, while the 216t coding for a aa truncation enabled virusinduced cell fusion, but failed to complement for infectious virus production ... infectious virus production and virus- induced cell fusion The results suggest that virus- induced cell fusion mechanisms are not required for cytoplasmic virion envelopment Materials and methods Cells ... for virus egress and cell- to -cell fusion [28,29] Recently, we delineated via site-directed mutagenesis the functional domains of UL20p involved in infectious virus production and virus- induced cell...

Ngày tải lên: 18/06/2014, 18:20

... simultaneously analyze 135 different cell surface antigens (128 cluster of differentiation antigens plus other surface antigens) on peripheral blood CD4+ and CD8+ T cell subsets from HIV+ and HIV- ... technology, by identifying new cell surface antigens that may potentially be associated with HIV disease stages Simultaneous screening for a large number of cell surface antigens revealed that changes ... of cell membrane receptors for cytokines and chemokines were more pronounced in CD4+ T cells Results The pairwise comparisons of groups identified 17 statistically differential cell surface antigens, ...

Ngày tải lên: 13/08/2014, 05:22

... CD28changes in the PBMC cell surface antigens in HIV patients on HAART: (A) CD2, CD3epsilon, CD5 and CD95; (B) Time related and CD36 Time related changes in the PBMC cell surface antigens in HIV patients ... to HAART, cell surface expression of adhesion molecules and levels of circulating adhesion molecules requires further investigation Three cell surface antigens were associated with cell activation ... and its expression on CD8+ T cells identifies the mature cytolytic effector cells [37,38] CD57 expression on CD8+, CD4+ T cell and NK cells is a general marker of cell proliferative inability...

Ngày tải lên: 13/08/2014, 06:20

... sensitivity of cell surface transported Lassa virus Figure glycoprotein Glycosidase sensitivity of cell surface transported Lassa virus glycoprotein At 48 h post-transfection, Vero cells expressing ... also transported to the cell surface of SKI-1/S1P deficient cells (data not shown) The finding that only an endo H-sensitive form of Lassa virus GP-C was found at the cell surface suggests that ... N-glycosylation sites of Lassa virus GP-C for intracellular trafficking we analysed cell surface expression of the mutated glycoproteins using a biotinylation approach Transfected Vero cells were labelled...

Ngày tải lên: 20/06/2014, 01:20

... virus and the cell surface The options to use unpurified viruses, low MOI and cell numbers indicate the opportunity to study virus host cell interaction even when the amount of cells and viruses are ... between virus and cells even when low amounts of cells or viruses are available Investigating viral binding using labeled and purified viruses is usually conducted with high virus to cell ratio, ... is a characteristic feature of many enteroviruses including enterovirus 70 and echovirus [13,14,21-25] Interactions between a virus and the host cell surface are generally studied using purified...

Ngày tải lên: 12/08/2014, 04:21

... the cases Virus culture was done for adenovirus, influenza A and B viruses, parainfluenza virus (PIV) types 1-3, RSV, enteroviruses, RV and human metapneumovirus (hMPV) [4,24] Viral antigens were ... RV: rhinovirus; RSV: respiratory syncytial virus; RNA: ribonucleic acid; Th: T-helper cell; Treg: T-regulatory cell; IgE: immunoglobulin E; hMPV: human metapneumovirus; PIV: parainfluenza virus; ... detection of entero- and RV, RSV, coronaviruses (229E, OC43, NL63 and HKU1), hMPV, human bocavirus, influenza A and B viruses, adenovirus and PIV 1-4 All rhino-enterovirus PCR positive samples could...

Ngày tải lên: 12/08/2014, 14:20

... measured in NaF-treated intact cells results from the cleavage of the disaccharide at the cell surface by an extracellular ‘acid trehalase’ pool [14] The cell- surface localization accounts for ... place in this subcellular compartment In contrast, targeting this enzyme at the cell surface is indispensable for growth of yeast cells on trehalose Ath1 domains relevant for cell- surface targeting ... found at the cell surface Controversy concerning the localization of Ath1 has been raised in two recent papers In a previous study, we suggested a localization for Ath1 at the cell surface based...

Ngày tải lên: 18/02/2014, 06:20

... starvation stress induced limited cell growth and death in SP1 cells (Fig 2Ac), yet treatment with lm LD inhibited this cell death (Fig 2Ad) Furthermore, as determined using a cell proliferation ... or inhibited cell death induced by serum starvation Next, we investigated the participation of Cer in cell survival promoted by PPARb, by examining intracellular Cer levels Total cellular lipids ... reduced cell survival and enhanced apoptosis induced by serum starvation [5] Although these previous reports suggest that CerK and C1P are involved in the regulation of cell survival or cell proliferation,...

Ngày tải lên: 18/02/2014, 18:20

... the surface of a variety of cell lines [42,48,52,55,56] and on the surface of endothelial cells [57,58] To address whether nucleo4674 lin is also present on the surface of developing muscle cells, ... the surface of myotubes (arrow in Fig 6D), as well as on the surface of nonmyosinexpressing cells Some of the punctate localization also occurred at the cell cell interface between these cells ... and incubated with the cells for h at room temperature Cells were washed, mounted and analyzed as described above To semipermeabilize cells for intracellular staining, cells were incubated in...

Ngày tải lên: 19/02/2014, 05:20

... control cells (B, C) Cells incubated with Glu-Pg: (B) residual adherent cells; (C) detached cytospun cells (D, E) Cells incubated with Glu-Pg and 0.5 lM of mAb A10.2: (D) residual adherent cells; ... cytospun cells (F) Cells incubated with Glu-Pg and 0.5 lM of mAb 34D3 No cells were recovered after cytospining the supernatant of control and Glu-Pg ⁄ mAb 34D3-treated cells *P < 0.0001 vs control cell- bound ... control plasmin; lane 3, untreated control cells; lane 4, Glu-Pg-treated cells; lane 5, Glu-Pg- and mAb A10.2-treated cells; lane 6, Glu-Pg- and mAb 34D3-treated cells Bands in lanes and correspond...

Ngày tải lên: 20/02/2014, 01:20

... Sertoli cell cultures (2525 ‹ 257 and 2538 ‹ 246 ng per well in three different cell cultures) Cell surface HSPG are bFGF partners during Sertoli cell postnatal development Developing Sertoli cells ... In conclusion, bFGF requires cell surface HSPG for the inhibition of steroidogenesis in developing Sertoli cells Cell surface HSPG are bFGF targets during Sertoli cell postnatal development As ... Sertoli cells Our present study demonstrates that bFGF in¯uences this expression in 20-day-old-rat Sertoli cells During testicular development, the physiology of Sertoli cells is modi®ed The cell...

Ngày tải lên: 21/02/2014, 03:20

... membrane to the nucleus 1.2 Signal Processing Cell communications with other cells and the extracellular matrix involve cellsurface proteins, such as receptors, cell adhesion molecules, ion carriers, ... any multicellular organism, the body’s cell communicate Cell signaling enables the coordination of cell activities, from basic life to tissue development and remodeling These basic cellular activities ... hand, surface signaling, signaling during endocytosis into the receiving cell and receptor recycling for further delayed signaling are crucial steps of cell signaling In polarized cells, the cell...

Ngày tải lên: 05/03/2014, 22:21

... and cell- surface expression but is unable to regulate cellular iron uptake like the wild-type HFE protein [4,15] Although much insight into HFE function has been gained through studying the cellular ... number of cells displaying this ERC/punctate pattern simultaneously displayed reticular localization in the same cells (50 ± 2% of N110A-expressing cells, 50 ± 2% of N130A-expressing cells and ... of these cells simultaneously displaying reticular localization (55 ± 7% of NN110/130AA-expressing cells, 51 ± 4% of NN130/234AA-expressing cells and 67 ± 7% of NN234/110AA-expressing cells, n...

Ngày tải lên: 06/03/2014, 22:21

... increase of migrating cells in response to receptor agonists over cell migration in the absence of agonists *P < 0.01; significantly increased cell migration as compared with cell migration in the ... chemotactic agonists Levels of cell- surface expression of the wide-type and chimeric formyl peptide receptors were examined by measuring the capacity of HEK293 cells transfected with the receptor ... of parental HEK293 cells [16] (and data not shown) The specificity of the peptide agonists on FPR and FPRL1 was tested on cells transfected with wild-type receptors HEK293 cells expressing FPR...

Ngày tải lên: 16/03/2014, 18:20

... the surfaces of endothelial cells The presence of the F1 b-subunit in retinoblastoma Y-79 cell surfaces is consistent with previously reported expression of F1Fo-ATP synthase in tumor cell surfaces ... synthase on HUVEC surfaces, leading to inhibition of migration and proliferation of endothelial cells [30–32] HUVECs possess high ATP synthesis activity on the cell surface [33] Extracellular ATP ... a protein(s) at the surface of endothelial cells To determine whether the endothelial PEDF-binding component was related to cell- surface F1Fo-ATP synthase, we prepared subcellular fractions of...

Ngày tải lên: 22/03/2014, 21:21

... number of cell surface receptors was assessed by binding of an anti-LDL-R monoclonal Ig (IgGC7) at °C A 1-h treatment of HepG2 cells with PMA resulted in a 30% decrease in the number of cell surface ... mean decrease in cell surface receptor number following PMA treatment was also seen in CHO cells transfected with human LDL-R (CHO-715); however, in these cells the number of surface receptors ... overexpression Furthermore, the cell surface cleavage mechanism appeared more sensitive to PMA regulation in HSF than in CHO cells Enzymes responsible for ectodomain shedding of cell surface proteins have...

Ngày tải lên: 23/03/2014, 12:20

... 3) Kinetics of Bax -induced cell death varied accordingly to the extent of Bax -induced mitochondrial lipid oxidation In the minimal medium without dioctylphtalate, Bax -induced cell death kinetics ... involved in cell death kinetics Fig Respiration -induced cis-parinaric acid oxidation in isolated mitochondria Mitochondria were isolated from control cells and GAL1/GAL10-driven Bax-expressing cells ... such as heavy metals -induced stress [40] We thus investigated a possible involvement of lipid oxidation in Bax -induced cell death Phospholipids were extracted from both whole cells and isolated...

Ngày tải lên: 23/03/2014, 21:20

... senescence Mechanisms of ERK -induced senescence Cellular senescence is an irreversible form of cell cycle arrest that prevents proliferation of damaged cells or cells that have surpassed their ... hallmarks of ERK-mediated cell death: sustained and sequestered activity ROS as mediators of ERK -induced cell death In the majority of the studies related to cell death induced by the Ras ⁄ Raf ... pathway -induced senescence by inactivating effectors of senescence, such as p53 or p16 ⁄ INK4A, mechanisms involved in ERKinduced cell death might also be silenced in tumor cells Tumor cells with...

Ngày tải lên: 29/03/2014, 08:20

... binding the cell- surface expressed nucleolin leading to the inhibition of HIV-attachment to the cell surface PTN binding to cells involves high and low affinitybinding sites even in cells that ... a receptor is the cell surface- expressed nucleolin Accordingly, by confocal immunofluorescence laser microscopy, we show that cell- surface bound PTN colocalizes with that of surface- expressed ... that PTN binding and internalization in cells is mediated by the cell- surface nucleolin Cross-linking of surface- bound PTN results in the clustering of surface nucleolin In general, the cross-linking...

Ngày tải lên: 30/03/2014, 20:20