validation of differential gene expression results by qrt pcr
báo cáo khoa học: " Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues" docx
Ngày tải lên :
12/08/2014, 03:20
... validation of expression profiles RT PCR4 RT PCR validation of expression profiles Reverse transcription-polymerase chain reaction (RT PCR) was used to validate the nectary-enriched expression profiles ... Comparison of gene expression in different nectary types Comparison of gene expression in different nectary types The number of genes displaying a two-fold or greater difference in expression ... expressed genes (e.g., see Figure and Additional file 11), with 45 of these genes displaying nectary-enriched expression profiles (see Additional file 7) Members of the YABBY transcription factor gene...
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Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
Ngày tải lên :
19/02/2014, 05:20
... E-cadherin gene mutation [7], modification of b-catenin [8], or the cleavage of E-cadherin by matrix metalloproteases (MMPs) [9] Cleavage of E-cadherin results not only in the disruption of cell–cell ... indicated, followed by western blotting for Shc, phospho-Erk and total Erk levels (C) Rescue by ectopic Shc isoform expression of Erk activation that was suppressed by down-regulation of endogenous ... Decma-induced Erk phosphorylation Moreover, expression of silent mutants of Shc isoforms showed that only p46Shc and p52Shc rescued the effect of the siRNA Overexpression of p66Shc not only failed to rescue...
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Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx
Ngày tải lên :
07/03/2014, 00:20
... inhibitory effect of asparagine and histidine on the expression of ASNS and that of leucine on CHOP (also known as GADD 153) gene expression (Table 1) Indeed, the first identification of a sequence ... the stimulation of gene expression by the presence of amino acids, only one gene, Pept 1, encoding a peptide transporter, was shown to contain an AARE-like sequence activated by phenylalanine ... presence of amino acid(s) resulted in inhibition of the DNA binding of the involved transcription factors By contrast, the presence of amino acid may also result in stimulation of the DNA binding of...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
Ngày tải lên :
17/03/2014, 10:20
... N (1999) Genetic analysis of nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expression of nif genes J Bacteriol ... chromosome of RAS46 by side-specific recombination The respective chromosomal mutations were confirmed by PCR and Southern blot analysis [29] For generation of homologous primers for PCR amplification, ... anaerobic conditions and expression of NifL and NifA was induced from the tac promoter (Ptac) with 10 lM IPTG Expression of nifH¢-lacZ¢ was monitored by the determination of the b-galactosidase...
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Báo cáo Y học: Prediction of temporal gene expression Metabolic optimization by re-distribution of enzyme activities ppt
Ngày tải lên :
17/03/2014, 10:20
... (shortterm) goal of genetic regulation [23,24] Dealing with the evolutionary optimization of gene expression in mathematical terms requires substantial simplications in view of the complexity of cellular ... extended versions of the unbranched pathway model by including in some detail transcription of genes, translation of mRNAs, and proteolysis In these models the genes may exist in ễOnế or ễOffế-states ... enzymes of the central metabolism of yeast resulting in down-regulation of glycolysis and up-regulation of the TCA-cycle and gluconeogenesis [1,3] In this paragraph we report on the application of...
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Báo cáo khoa học: Differential gene expression profiles of red and green forms of Perilla frutescens leading to comprehensive identification of anthocyanin biosynthetic genes doc
Ngày tải lên :
23/03/2014, 07:20
... regulation of the expression of biosynthetic genes (regulatory genes) [4,9–11] However, the coverage of gene expression profile by cDNA -differential display seems still incomplete, given the lack of a ... crude drug Differential gene expression analysis of the two forms by cDNAdifferential display led to the identification of several genes involved in biosynthetic reactions (structural genes) and ... confirmed by experiments The expression pattern of the PfGST1 gene was investigated by semiquantitative RT -PCR for the RNA from leaves and stems of red and green perilla (Fig 3) Predominant expression...
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Báo cáo hóa học: " Differential Gene Expression to Investigate the Effects of Low-level Electrochemical Currents on Bacillus subtilis" doc
Ngày tải lên :
20/06/2014, 23:20
... follow the kinetics of viability and gene expression over time In summary, we conducted a detailed study of the effects of weak DC on viability, gene expression and morphology of B subtilis The ... products and the results of their interactions with the components of LB medium The AFM results suggest that the killing of bacterial cells by DC is not through direct physical forces of the currents ... and gene expression of B subtilis were studied There was less killing of biofilm cells by incubating in the pre-treated media than when the current was directly applied, especially for biofilms...
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Báo cáo y học: "Gene expression induced by interleukin-17 in fibroblast-like synoviocytes of patients with rheumatoid arthritis: upregulation of hyaluronan-binding protein TSG-6" doc
Ngày tải lên :
09/08/2014, 01:22
... by scanning densitometry with the software Scan Pack version 3.0 (Biometra) as an IL-17-target gene The expression of the oncogene c-myc was downregulated The differential expression of the genes ... TSG-6 mRNA expression [% of control; control = 100 %] IL-17-induced gene expression Fibroblast-like synoviocytes were cultured for 24 hours in the presence of IL-17 (20 ng/ml) Results of quantitative ... Combinations of IL-1β and IL-17, or TNF-α and IL-17, showed additive effects on TSG-6 expression of 13.6-fold and IL-17 stimulates the expression of TSG-6 mRNA (a) Time course of TSG-6 mRNA expression...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
Ngày tải lên :
09/08/2014, 01:23
... amplification efficiencies of the target and housekeeping gene are identical [23] The limitations of the C(t) methods of Q -PCR led us to consider a set of standard curves by which the assay results could ... influence, the CE values of the target genes are normalized to a reference gene using the CE for GAPDH The RE compared with GAPDH is determined by dividing the CE of the target gene by the CE for GAPDH ... chemistry and Q -PCR as described in Materials and methods Figure shows that the stimulated PBMC are a source of mRNA for the target genes of interest The expression of other relevant genes, including...
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Báo cáo y học: "Differential gene expression of bone anabolic factors and trabecular bone architectural changes in the proximal femoral shaft of primary hip osteoarthritis patients" pptx
Ngày tải lên :
09/08/2014, 08:23
... between gene expression and bone architecture were explored The results of the data are indicative of the generalised skeletal distribution of primary OA pathology and suggest that gene expression ... biosynthetic activity [18] The lack of differential gene expression of these anabolic growth factors in our study is most likely due to the gene expression being contributed by the various cell types present ... of COL1A2 gene expression, the level of COL1A1 gene expression in OA was almost double that in the controls (1.71 versus 0.91, respectively) In comparison to the controls, IGF-II/GAPDH mRNA expression...
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Báo cáo y học: "Analysis of adenovirus trans-complementationmediated gene expression controlled by melanoma-specific TETP promoter in vitro" ppsx
Ngày tải lên :
12/08/2014, 04:20
... as strong and specific delivery of therapeutic genes is one of the main goals of cancer therapy, it may be of importance for the general usage of the binary Ad expression system to investigate ... CD40L gene, no consistent influence of the IL-2 gene position was noticed for IL-2 expression in the two different cell types Of note also, detection of low IL-2 amounts in supernates of HeLa ... cell type-specific transgene expression Enhancement of GFP transgene expression by adenovirus trans-complementation To determine concentration-dependence and kinetics of Ad trans-complementation-mediated...
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Báo cáo y học: "Silencing CD36 gene expression results in the inhibition of latent-TGF-β1 activation and suppression of silica-induced lung fibrosis in the rat" pdf
Ngày tải lên :
12/08/2014, 14:20
... 10:36 Figure detected by realtime -PCR CD36 mRNA levels from the AMs of each group were CD36 mRNA levels from the AMs of each group were detected by realtime -PCR The expression of CD36 mRNA in the ... 5'-CGCTCGGTCAGGATCTTC-3'), were synthesized by Genechem (China) Real-time quantitative PCR (qRTPCR) analysis was performed as previously described Each PCR reaction mixture (20 μl) contained 10 μl of × SYBR Green Master ... percentage of active TGFβ1 Therefore, we believe that Lv-shCD36 could inhibit activation of L-TGF-β1 by decreasing expression of CD36 on the membrane of AMs to further reduce the combination of CD36...
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Báo cáo y học: "Regulation of chondrocyte gene expression by osteogenic protein-1" ppt
Ngày tải lên :
12/08/2014, 15:22
... Grouping of genes was done by computing over-representation of regulated genes in gene ontology (GO) classes [14] Statistical analysis consisted of 1) analysis of differentially expressed genes ... the expression of genes in the IL-6 signaling pathway, we found that OP-1 not only regulates expression of the IL-6 family of cytokines but also B Changes in gene expression of IL-6 family of ... stimulated expression of insulin-like growth factor (IGF)-1 and IGF-1 receptor genes [17], while inhibition of OP-1 gene expression by OP-1AS down-regulated mRNA expression of these genes We have...
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Báo cáo y học: "Effects of short-term glucocorticoid treatment on changes in cartilage matrix degradation and chondrocyte gene expression induced by mechanical injury and inflammatory cytokines" doc
Ngày tải lên :
12/08/2014, 18:20
... Effects of DEX on iNOS gene and protein expression and nitrite production A iNOS relative gene expression Values at the y-axis represent the expression level of each gene normalized to that of the ... Gift of Hope Organ and Tissue Donor Network (Elmhurst, IL, USA), approved by the Office of Research Page of 15 Affairs at Rush-Presbyterian-St Luke’s Medical Center and the Committee on Use of ... mechanistic pathways by which DEX may suppress cartilage degradation The pathways of interest included regulation of aggrecanase gene expression and the activation of aggrecanases by proprotein convertases,...
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Báo cáo y học: "Differential gene expression in anatomical compartments of the human eye" pptx
Ngày tải lên :
14/08/2014, 14:22
... results of the disease gene analysis, including the list of candidate genes for each disease genes for for disease Results of diseases genetic intervals The list intervals used in and Compartment gene ... aspects of ocular pathology deposited research One direct application of the gene expression patterns we have defined is the identification of candidate genes for genetic diseases that differentially ... molecular level by identifying clusters of differentially expressed genes, or 'gene signatures', characteristic of each eye compartment We also wanted to investigate how gene expression varies...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
Ngày tải lên :
02/11/2012, 11:08
... Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling Nature 2000; 403: 503-511 Alizadeh AA, Staudt LM Genomic-scale gene expression profiling of normal and malignant ... upregulated genes there were the a-myb oncogene, the interleukin receptor gene (IL4R), the gene of the transporter protein TAP2, the gene of protease calpain small subunit (capn4) and other (in case of ... SalI-fragments of cDNA clones generated by subtraction combined with differential screening or with [32P]-labeled PCRfragments of the corresponding genes As a control, we used a [32P]-labeled β-actin PCR- amplification...
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Báo cáo khoa học: Differential gene expression in periportal and perivenous mouse hepatocytes potx
Ngày tải lên :
23/03/2014, 10:20
... compilation ª 2006 FEBS 5053 Zonal gene expression in mouse liver A Braeuning et al Table Validation of microarray analysis data by real-time RT -PCR If genes are represented by more than one probe set ... Perivenous expression is indicated by green, and genes with preferential periportal expression are indicated by red (A) Perivenous zonation of glycolysis and periportal zonation of gluconeogenesis ... analyzed by real-time RT -PCR The PCR data closely resembled the findings of the microarray analysis A comparison of the results obtained with the two methods is shown in Table Differences in genes...
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Báo cáo khoa học: Ablation of Sax2 gene expression prevents diet-induced obesity docx
Ngày tải lên :
28/03/2014, 23:20
... food intake by suppressing the expression of NPY mRNA and by activating POMC mRNA expression, whereas low levels activate NPY mRNA expression, which in turn inhibits the expression of POMC mRNA, ... are indirectly affected by a lack of Sax2 gene expression, most likely through an impairment of food absorption 372 Results Comparison of body weight and food uptake of adult wild-type, Sax2 ... directly affected by lack of Sax2 gene expression A B C D E F Fig Analysis of blood glucose, insulin and leptin levels of female Sax2+ ⁄ + and Sax2) ⁄ ) animals (A) Blood glucose levels of Sax2+ ⁄ +...
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báo cáo hóa học:" Cyclic changes in gene expression induced by Peg-interferon alfa-2b plus ribavirin in peripheral blood " pot
Ngày tải lên :
18/06/2014, 15:20
... time of the 10 genes most differentially expressed, plotted as percentage of maximum signal with pointsexpression profileby natural cubic splines Gene were Gene expression profile over time of ... of expression across time-points, data from the top 90 genes (by p-value) across all time points were To illustrate the pattern of expression across time-points, data from the top 90 genes (by ... applicable; No fold change cutoff applied † Not applicable plus ribavirin on gene expression as a function of time in a cohort of patients infected with HCV genotype The number of genes modified and the...
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Báo cáo hóa học: " Inhibition of cytokine gene expression and induction of chemokine genes in non-lymphatic cells infected with SARS coronavirus" doc
Ngày tải lên :
20/06/2014, 01:20
... chemokines by the infected tissue cells Results Virus-induced cytokines not only play a significant role in host defense, but also in immunopathogenesis Investigations of the cytokine profiles of SARS ... Interferon-stimulated genes Interferon-stimulated genes RNA samples of Caco-2 cells (A) and HEK 293 cells (B) described in Fig were investigated by RT -PCR for the presence of ISG56 and MxA mRNAs ... 20 µl of 1×RT buffer (Invitrogen) supplied with mM each of the four deoxynucleotide triphosphates, 20 U of RNasin, and 10 mM dithiothreitol The resulting cDNA was amplified by 35 cycles of PCR, ...
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