v2 v3 region of the 16s rrna gene

... Effects of deletion of the GTPase domain on the 16S rRNA- binding activity of Era Results Effects of the deletion of the RNA- and membranebinding domains of S pneumoniae Era on the GTPase activity of ... previously, the KH domain of Era is required for the binding of the protein to 16S rRNA, and part of the domain is also required for the binding of the protein to membrane Thus, the regions critical ... that of the full-length Era proteins The C-terminal parts of both Era proteins were able to bind to 16S rRNA in the absence of the N-terminal GTPase domain and the amount of the RNA bound to the...

Ngày tải lên: 31/03/2014, 07:20

... probably also the case for other cultured fish Therefore it could be possible to lower the amount of fish meal, one of the major components of fish food, in the food for these fish Furthermore, it ... important for the health of the animal and therefore this study could be relevant for aquaculture Furthermore, the presence of different nitrogen cycling bacteria in the GI tract of fish could offer ... diversity of the microbiota in the GI tract of common carp Obtained 16S rRNA gene sequences were used to classify the different microorganisms present in the fish gut and here we will also discuss the...

Ngày tải lên: 20/06/2014, 23:20

... amplification of 16S rRNA gene inserts After cloning of the 16S rRNA gene products amplified by PCR for each sample, 50 clones from each generated library were randomly selected The 16S rRNA gene insert ... culture of the same samples The clinical interest of this study is the presence of any organism on the prosthetic hip joints and the role, if any, that they have in initiating, pro- Page of 11 ... that PCR amplification of the 16S rRNA gene, a highly conserved region within the bacterial genome, is invaluable in the detection of the bacterial types involved in prosthetic hip joint infections...

Ngày tải lên: 09/08/2014, 10:20

... indicate the number of patient set from whom bacteria were detected aAccession number of the bacterial species in the EMBL database bLength of alignment on which the 16S rDNA inserted sequence and the ... valuable overall picture of the bacterial DNA environment present in the SF of the actively inflamed joints of arthritis patients Characterization of the DNA reveals a wide spectrum of organisms so far ... system PCR was positive in 20 of the 27 (74.10%) patients indicating the presence of bacterial 16S rDNA within the synovial samples Of note, PCR was positive in five out of five (100%) patients with...

Ngày tải lên: 09/08/2014, 14:22

... Cloning of the 5¢-upstream region of the Pokemon gene To identify the regulatory sequences that control expression of the Pokemon gene, a 2204-bp section of the 5¢-upstream region of the Pokemon gene ... DNA as the Analysis of upstream region of the Pokemon gene template Figure shows the nucleotide sequence of the 2204-bp promoter region and a short stretch of the transcription region The translation ... determine the impact of the length Analysis of upstream region of the Pokemon gene between the NEG-U and NEG-D elements on the regulation of the Pokemon promoter, we inserted the NEG-U element into the...

Ngày tải lên: 30/03/2014, 04:20

... directly the role of CHD3 in the regulation of PS1 gene in vivo We first 1440 Fig Effect of N-terminal deletions of CHD3 on the inhibition of transcription by CHD3 and on the binding of CHD3 with ... indicating the crucial importance of these two Ets motifs [15] In addition to controlling the level of gene expression, Ets factors may direct the choice of the promoter elements in play, and therefore, ... CHD3 CHD3 represses the transcription of PS1 in SH-SY5Y neuronal cells We tested the effects of pC1.CHD3 on expression of the PS1 gene in SH-SY5Y cells We compared the action of the C-terminal fragment...

Ngày tải lên: 30/03/2014, 09:20

... complementary to the conserved regions of the 16S rRNA gene fragment (116 bp) of both Ehrlichia spp and Anaplasma spp were used The composition of PCR mix, reaction conditions and the sequence of PCR probe ... with 16S rRNA gene sequence of the Arkansas (AF416764), the Sapulpa (U60476) and the 91HE17 (U23503) strains of E chaffeensis, all of these originate from the USA We also performed phylogenetic ... region of 16S rRNA gene were used [1] The PCR mix for the first round PCR consisted of 2.5 µl of 10X PCR buffer, 2.5 µl of 25 mM MgCl , µl of 2.5 mM deoxynucleoside triphosphate (dNTPs), 2.5 U of...

Ngày tải lên: 07/08/2014, 18:21

... ANRr S61 a roF tcnitsid ylhgih saw nrettap noitcirtser eht hguoht ,devresnoc ylhgih ton era gnidnib remirp rof secneuqes tegrat eht taht seilpmi siht ;desu erew sremirp fo stes ruof ,]61[ tnemirepxe ... esoraga morf deifirup dna desicxe erew pb 715 erew taht stcudorp RCP nim rof Co27 ta pets noisnetxe lanif a htiw ,s 03 rof Co27 sretcarahc dlob ni nwohs era stnemgarf noitcirtser euqinU** sleg ... eht ,leg esoraga %3 gnisu nehw ,revewoH troffe dna emit erom seriuqer dna ,gnidaer dna gnildnah htiw seitluciffid yb detacilpmoc si PLFR-RCP ANRr S32 rof gniniats etartin revlis htiw sleg edimalyrcaylop...

Ngày tải lên: 07/08/2014, 18:21

... histochemical assays, the 5’upstream region of the pine gene was able to drive gene expression in pine cotyledons To further characterize the function of the 5’upstream region of the pine GS1a gene, stable ... plasmid pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing 2.2 Fusions of the 5 region of GS1a to the GUS reporter gene The 981 bp sequence upstream of the translation codon ... half of the gene including part of the coding region organized in seven exons, interrupted by introns and 980 bp upstream the translation initiation codon (figure 1) The sizes of introns in the...

Ngày tải lên: 08/08/2014, 14:20

... modulating the expression of the CD154 gene As with other cytokine genes, the 3' UTR of the CD154 mRNA contains binding sites for RNA–protein complexes that are responsible for the lability of the mRNA ... expected, the half-life of the 24CAs mRNAs was higher than that of the rest of the alleles after and 24 hours of anti-CD3 stimulation As shown in Figure 2a, scattering of the data in the non-24CAs ... study we describe the association of the microsatellite in the 3' UTR of the CD154 gene with RA in females from the Canary Islands The 24CAs allele is less represented Page of 11 (page number...

Ngày tải lên: 09/08/2014, 10:21

... with the Bv 1v3 chimera, the BR(N283T) mutant and the DS17 and DS17(N283T) The Bv 1v2 chimera containing the V1 /V2 region of the brain Env in the context of the spleen Env mediated infection of macrophages ... only the V1 /V2 gp120 fragments in the context of the spleen Env (named Bv 1v3 and Bv 1v2, respectively) and the spleen V1 /V2- C2 -V3 or only the V1/ V2 gp120 fragments in the context of the brain Env ... for the role of N283 and T283 on the phenotypes observed the identity of the gp120 and gp41 expressed from each construct Furthermore, chimeric Env containing the brain V1 /V2- C2 -V3 or only the...

Ngày tải lên: 13/08/2014, 05:21

... distance Figure Periodicity of CpG sites in the 500-bp region at the 5' portion of the intron Periodicity of CpG sites in the 500-bp region at the 5' portion of the intron Counts of each distance from ... representation of the first intron of eutherian Impact Schematic representation of the first intron of eutherian Impact The GenBank accession number and length are listed to the right of the common ... primer-walking method The identities of these amplicons as the Impact gene were confirmed by the 30-nucleotide sequences at the beginning of exon This short region was also amplified along with the first...

Ngày tải lên: 14/08/2014, 21:20

... level of fruits and green vegetables in the diet of the Egyptian population in general However, the lack of correlation between the plasma tHcy and the folate could be explained by the presence of ... 192 Figure The effect of the T allele on the plasma homocysteine in the different studied groups Bar chart illustrating the effect of the T allele on the level of homocysteine in the different ... each genotype in the three different groups There was no significant difference in the occurrence of the TT genotype in any of the studied groups (P>0.05) Also the prevalence of the different genotypes...

Ngày tải lên: 03/11/2012, 09:49

... e.g the case of the association between the N-terminal region of the membrane-bound tyrosine kinase Lck with the cytoplasmic tail of the T-cell coreceptors CD4 or CD8 [37] In solution, on the ... (supplementary Figure S1) The lack of chemical shift dispersion in the HN region as well as in the methyl region (data not shown) is an indicator of the lack of globular structure, and of little, if any, ... shown) The lack of significant chemical shift dispersion in the HN and Ha chemical shifts is an evidence of lack of tertiary structure On the other hand, NMR spectra suggest that the conformation of...

Ngày tải lên: 18/02/2014, 16:20

... To understand the regulation of KLK11 gene expression, we investigated the KLK11 gene promoter regions Here we report that the KLK11 gene has three promoter regions accompanying the corresponding ... a part of isoform mRNA The third exon, exon 1c, extended from +1379 to +1454 The noncoding exons of exons 1a and 1c contributed to the 5¢ nontranslated region of isoform mRNA in which the common ... KLK12 gene The promoter region of the KLK11 gene is believed to lie within this region, although we cannot rule out that the regulatory sequence for KLK11 expression may exist within the KLK12 gene...

Ngày tải lên: 19/02/2014, 06:20

... induction of the COX-2 gene by pro-inflammatory stimuli Discussion The regulation of COX-2 expression during inflammation has been the focus of numerous studies The heterogeneity of parameters such as the ... observed across the different studies as well as the similarity of the recognition sites of the NFAT and Ets factors led us to investigate the potential involvement of the epithelial specific ... transcription factors as the mutation of the CRE site within the COX-2 promoter or the expression of a dominant negative mutant of CREB reduces inducibility of the COX-2 promoter [9,12–14] The transcription...

Ngày tải lên: 19/02/2014, 17:20

... removal of the second half of the PolC N-terminus would help to improve the results further Therefore, we took only the fragment of the multiple sequence alignment covering the first half of the PolC ... explore the structure of the PolC N-terminal region We found that this region includes two consecutive structural domains Both domains are distantly related to the structure of domain V of the clamp ... Interestingly, the first half of the PolC N-terminal region ( 1–100) also detected the PolIIIs-V domain, albeit weakly (hhsearch probability of 16%) The structures of PolIIIs-V and DnaA-I adopt a variant of...

Ngày tải lên: 05/03/2014, 23:20

... FnBRs of FnBPB A panel of mouse mAbs was produced against the recombinant repetitive region of FnBPB Analysis of mAbs binding to the recombinant FnBR indicated the generation of two categories of ... in the presence or absence of Fn (or the NTD), all of the mAbs showed strong anti-LIBS activity However, these results not rule out the possibility that the immune system of the host might generate ... the ligand to the other repeats that share a common epitope Other inhibition mechanisms cannot be excluded; among these, there is the possibility that, through the effect of the proximities of...

Ngày tải lên: 06/03/2014, 22:21

... (1994) Identification of a GC element in the promotor of the murine L32 ribosomal protein L32 gene and the 3¢-terminal region of the human apolipoprotein A-I gene, capable of binding with murine ... affinities to the protein of interest In the case of the rpL32 fragment or the (GCC)9 sequence, there are several repeated ZF5-binding sites within a single oligonucleotide Therefore, the affinity of those ... target gene transcription Moreover, in silico content analysis of the core promoter regions of human genes revealed the presence of ZF5 consensus sites within approximately 60% of human gene core...

Ngày tải lên: 07/03/2014, 05:20

... showed that they did not contain the central region or the 5¢ end of the gene The cosmid clone containing the longest part of the new gene was named CAR1 and studied The 1719 probe and a cDNA probe ... experiments The last exon is composed of a coding sequence of 193 bp and of an untranslated region of at least 131 bp The size of the 24 introns ranges from 79 bp to about 16 kb The size of the largest ... was used in RT-PCRs with the sense oligonucleotide NAU941 from within the repeat region (see further) to obtain the cDNA corresponding to the end of the repeat region The sense oligonucleotide...

Ngày tải lên: 08/03/2014, 23:20