uv b radiation its effects and defense mechanisms in terrestrial plants

... tobacco BY2 cells (Hoque et al 2007) 2.2 Amino Acids, Proline, and Amides It has been reported that amino acids (such as alanine, arginine, glycine, serine, leucine, and valine, the nonprotein ... is suppressed and the expression of a certain set of genes including the luminal binding protein gene BiP is induced The underlying mechanisms of UPR in yeasts and mammals have been well researched ... Suprasanna UV-< /b> B Radiation, Its Effects and Defense Mechanisms in Terrestrial Plants Fernando E Prado, Mariana Rosa, Carolina Prado, Griselda Podazza, Roque Interdonato, Juan A González, and...

Ngày tải lên: 14/03/2014, 10:20

... inducible NF- B activation by inhibiting InBa kinase activation, thereby suppressing NF- B- regulated antiapoptotic and metastatic gene expression, up-regulating apoptosis, and inhibiting invasion ... SGC-7901 and BGC-823 by RT-PCR and western blotting (A) Expression of bARs in human gastric cell lines SGC-7901 and BGC-823 at the mRNA level by RT-PCR Both of cell lines expressed b1 - and b2 -AR ... of b1 - and b2 -ARs was determined at both the mRNA and protein level in SGC-7901 and BGC-823 cells by RT-PCR and western blot Our results showed that b1 - and b2 -adrenergic receptors could be detected...

Ngày tải lên: 09/08/2014, 09:20

... generated by irradiation of two rodent fibroblast and two human tumour cell lines in the presence and absence of chemical inhibitors of PARP activity The variable nature and magnitude of the effects ... hamster fibroblast cell lines indicated that, while 3AB significantly affected radiosensitivity parameters in CHO-K1 fibroblasts, any radiosensitising effects of NU1025 in V79-379A fibroblasts were ... read and approved the final manuscript References Methods Cell lines and chemical inhibitors T98G and U373-MG human glioblastoma cells and CHOK1 and V79-379A hamster fibroblast cells were routinely...

Ngày tải lên: 13/08/2014, 16:21

... calcium channel blocker and staurosporine, a serine-threonine kinase inhibitor, SB 203580, a P38 MAPK inhibitor, PD 98059, an ERKK inhibitor and SB 600125 JNK inhibitor The UV-< /b> Binduced H2O2 production ... whether suramin could inhibit the UV-< /b> Binduced medium alkalinization The results show that the UV-< /b> B- induced alkalinization was inhibited by suramin (Figure 1b) Suramin inhibited alkalinization of the ... response to UV-< /b> B irradiation and its inhibition by suramin Medium alkalinization of C roseus suspension cultured cells in response to UV-< /b> B irradiation and its inhibition by suramin (a)Sixday-old...

Ngày tải lên: 12/08/2014, 05:20

... surface water can be treated in a UV < /b> concentrating radiation System (Yves et al., 1996) In this process, major toxic compounds are mineralizaed to CO2, H2O and mineral acids such as HCl, instead of ... range (300-380 nm) and quartz tube was used for tube receiver because it has excellent UV < /b> stability and transmissivity Then, modules are connected in series and the water flow (5L/min) directly from ... 1999) and to reduce toxic metals (Cho et al., 2002) The widespread presence of chlorinated hydrocarbons such as trichloroethylene (TCE) and perchloroethylene (PCE) in both natural and drinking...

Ngày tải lên: 05/09/2013, 08:40

... readily incorporate into Myr2Gro-PGro bilayers in the liquid-crystalline state and remain incorporated in the gel state Permeabilization of phospholipid bilayers by GS ring-size analogs In order ... presumably due to its expanded ring; in contrast, GS12 exists in a distorted b- sheet and b- turn structure and is conformationally much more flexible [5,16,28] Although the intrinsic hydrophobicities ... previously by assuming that in disordered GS analogs, the absence of the interstrand hydrogen bonds present in the b- sheet ring results in solvation of the amide NH and CO groups, in turn causing a...

Ngày tải lên: 21/02/2014, 01:21

... O2 rebinding and the 6110 quantum yield of BR for the a and b subunits within the liganded dimer and tetramer of hemoglobin were determined Second, pH and NaCl effects on the total protein affinity ... different types of subunits within considered proteins Rates of O2 binding to the a and b subunits within liganded hemoglobin measured over the pH range of the alkaline Bohr effect The bimolecular oxygenation ... hemoglobin binds chloride ions at high-affinity sites with an intrinsic binding constant of $ 10 m)1 The data [35,37] have Fig The parameters of O2 binding to the a and b subunits within liganded...

Ngày tải lên: 16/03/2014, 14:20

... anti-PHB ⁄ anti-rabbit-FITC staining; (D) merge Arrows indicate staining of PHB D proteins have been shown to be involved in endocytosis and vesicle recycling [53] In summary, we found that PHB is ... staining; (B) Mitofluor Red 589 (mitochondria) staining; (C) anti-His (exogenous PHB)-FITC staining; (D) merge The arrows indicate staining of His-tagged PHB in (C) or both PHB and Mitofluor in ... mitochondria (Fig 4B, C) A western blot of total cellular protein extracts using anti-PHB serum showed two bands in cells incubated with recombinant PHB, the top band corresponding to His-tagged PHB (Fig...

Ngày tải lên: 29/03/2014, 08:20

... observed in the NOS2 promoter Interestingly, in LPS +IFNg-treated glial cultures C/EBPb binding was observed in all four promoters analyzed and, in the case of the NOS2 promoter, C/EBPb binding ... increases in C/EBPb mRNA levels h after treatment, and in nuclear levels of both activating (Full/LAP) and inhibitory (LIP) C/EBPb isoforms 24 h after treatment The increases in C/EBPb mRNA and ... extent Both stimuli also induce C/ EBPb binding to proinflammatory gene promoters but this binding is stronger when induced by LPS+IFNg Lack of C/EBPb results in attenuated expression of proinflammatory...

Ngày tải lên: 19/06/2014, 22:20

... the other hand, OPG binds to RANKL and thereby inhibits osteoclast activation The osteoblast function can be described by alkaline phosphatase specific activity [15] To closer study our in- vivo ... debris on MG63 osteosarcoma cells in vitro J Bone Joint Surg Am 1999, 81:452-461 16 Villa I, Mrak E, Rubinacci A, Ravasi F, Guidobono F: CGRP inhibits osteoprotegerin production in human osteoblast-like ... contributions to conception and design, analysis and interpretation of data, have been involved in drafting the manuscript and revising it critically for important intellectual content, and have...

Ngày tải lên: 20/06/2014, 04:20

... the other hand, OPG binds to RANKL and thereby inhibits osteoclast activation The osteoblast function can be described by alkaline phosphatase specific activity [15] To closer study our in- vivo ... debris on MG63 osteosarcoma cells in vitro J Bone Joint Surg Am 1999, 81:452-461 16 Villa I, Mrak E, Rubinacci A, Ravasi F, Guidobono F: CGRP inhibits osteoprotegerin production in human osteoblast-like ... contributions to conception and design, analysis and interpretation of data, have been involved in drafting the manuscript and revising it critically for important intellectual content, and have...

Ngày tải lên: 20/06/2014, 07:20

... curves of absorbance at 460 nm (Abs460) and (b) plots of ln (Abs0/Abst) versus time in the presence of antioxidants (10 lM), where Abs0 is initial Abs460 and Abst is Abs460 at time t Vitamin E was ... pseudofirst-order rate constants kobs of b- carotene bleaching with linoleic acid peroxyl radical at 50°C Values of kobs were obtained by monitoring the absorbance of b- carotene aqueous solution (8.2 ... confirmed its formation (obtained as a brownish water solution including an excess of free c-CD) and determined the concentration of solution using a UV-< /b> vis spectrometer by comparison of the peak absorbance...

Ngày tải lên: 22/06/2014, 01:20

... of lumbar vertebrae Fusion of thoracic vertebrae Absence of ribs Fusion of ribs Bifurcation of ribs Shortening of ribs Displasia of sternebrae Missing of sternebrae Hypoplasia of sternebrae Curvature ... X -radiation in mice and some seasonal effects in the perinatal period Radiat Res 1965, 25, 611-625 Kimmel, C A and Trammell, C A rapid procedure for routine double staining of cartilage and bone in fetal ... of brain, skull and extremities 10 11 12 13 14 15 16 17 18 References Beir, V Health effects of exposure to low levels of ionizing radiation Biological effects of ionizing radiations Washington,...

Ngày tải lên: 07/08/2014, 14:23

... ionizing radiation Biological effects of ionizing radiations Washington, National Academy Press, 1990 Brent, R D The effects of ionizing radiation, microwaves, and ultrasound on the developing embryo: ... vertebrae Fusion of thoracic vertebrae Absence of ribs Fusion of ribs Wavy ribs Hypoplasia of ribs Displasia of sternebrae Missing of sternebrae Hypoplasia of sternebrae Curvature of tibia Absence ... b 1.41±0.12 3.53±0.13 a 1.13±0.05 b 0.80±0.05 48 b b 67(60.91) b 1.34±0.15 3.41±0.16 b 1.09±0.05 b 0.76±0.04 79.09 26.36 b 80(100) b 0.92±0.08 b 2.71±0.22 b 1.02±0.04 b 0.72±0.02 72.5 98.75 b...

Ngày tải lên: 07/08/2014, 15:20

... ,OR trebliG 02 5101-9001 ,97 ,6991 icS yriaD J dleiy klim tnelaviuqe erutam dna ytirap yb detceffa sa swoc nietsloH fo erocs noitidnoc ydob ni egnahC G etnattiB ,B oreitnoC ,L inoliaB ,R inavotnaM ... detaler eb ot demuserp si noitatcal ylrae gnirud ytirap desaercni dna erocs noitidnoc ydob neewteb noitalerroc esrevni ehT ]63,91[ srehto yb detroper neeb sah sa ,ytirap yb detavargga eb ot dnuof ... tnemecalpsid lasamobA mutraptsop skeew nihtiw denrecnoc remraf eht ro/dna nairaniretev a yb devresbo sngis lacinilc yb desongaid erew )sisotek ro ,revef klim ,tnemecalpsid lasamoba( sredrosid cilobateM ]52,5[...

Ngày tải lên: 07/08/2014, 18:21

... separated by SDS-PAGE under reducing conditions before transfer onto PVDFmembranes (Roth, Karlsruhe, Germany) Equal protein loading was confirmed by Ponceau S staining Blots were blocked in TBS buffer ... cell numbers and longer incubation time with WST-1 resulted in an increased absolute absorption and smaller error bars A completely different picture was obtained when testing the antineoplastic ... 6C and 6D) Taken together, our results show that the Akt-inhibitor ErPC3 increases radiation- induced apoptosis in prostate cancer cells The most prominent combination effects were obtained in...

Ngày tải lên: 09/08/2014, 09:20

... a trypsin inhibitor that balances concentrations of trypsin and also functions as a weak inhibitor of other serine proteinases [10,11] In addition, TATI has been found to be involved in tissue ... trypsin inhibitor (PSTI) and serine protease inhibitor Kazal type (SPINK1), is a biomarker of poor prognosis in colorectal cancer patients, both as assessed in tumour tissue (t-TATI) [1] and in ... trypsin inhibitor (TATI) using the streptavidin-biotin system J Immunol Methods 1993, 161:97-106 Goldstein NS, Bosler D: An approach to interpreting immunohistochemical stains of adenocarcinoma in...

Ngày tải lên: 09/08/2014, 09:21

... patients prior to and following rituximab treatment Changes in PB and BM leukocyte populations, Ig, and RF at baseline and following rituximab treatment are presented in Table At baseline, all the ... non-specific binding was blocked with 0.1% rabbit serum The cells were incubated with dye-conjugated monoclonal antibodies (mAbs), washed, resuspended in FACSbuffer (containing PBS, 1% FCS, 0,1% NaAz and ... P = 0.004) in BM, and by an increase of immature (CD27-IgD) B cells (P = 0.01) The absolute numbers of B cells in the rituximab-treated and tituximab-naïve patients are shown in Table No correlation...

Ngày tải lên: 09/08/2014, 14:22

... protein bands was scanned by densitometry using MyImage (SLB, Seoul, Korea), and quantified by Labworks 4.0 software (UVP Inc., Upland, CA, USA) The relative density of the DNA-protein binding bands ... No Ban et al Figure Abolition of the inhibitory effect of thiacremonone by DTT and glutathione GSH, and in the cells harboring mutant p50 on NO generation and DNA binding activation of NF- B binding ... target molecules in intracellular signal transduction proteins including NF- B through cysteine-cysteine interaction or other binding ways, and thus inhibit inflammatory responses and development...

Ngày tải lên: 09/08/2014, 14:22

... cells in RA has not been completely delineated In this study, we examined chemokine receptor expression by peripheral blood in both normal donors and subjects with RA, and also synovial B cells ... stained with monoclonal antibody (mAbs) to CD19 and inducible costimulator-ligand (ICOSL), B cell-activating factor receptor (BAFF-R) or transmembrane activator and CAML-interactor (TACI), and ... for 30 minutes Then, the × 105 B cells were incubated in 96-well with the indicated chemokines with or without pre-coated anti-IgM mAb (2 μg UHB; SouthernBiotech, Birmingham, AL, USA) in RPMI1640...

Ngày tải lên: 09/08/2014, 14:22