... (Ishikawa et al 2006) Ascorbate plays an important role in plants as an antioxidant and asa cofactor of many enzymes (Ishikawa et al 2006) As an antioxidant, ascorbate protects plants from oxidative ... Iqbal R Khan, Rahat Nazar, Asim Masood, and Shabina Syeed 413 429 Contents ix 21 Role of Salicylic Acid in Alleviating Heavy Metal Stress Losanka P Popova, Liliana T Maslenkova, Albena Ivanova, ... 201 0a) , and Brassica juncea (Ahmad 2010; Ahmad et al 2011) SOD activity has also been observed to increase by the application of heavy metals such as cadmium (Shah et al 2001; John et al 2009; Ahmad...
... i is an indicator which assumes the value zero for the control case, i.e radiation alone, and one for the drug-treated case; and δx – where "x" is any ofthe parameters above – is the variation ... growth-arrested populations of glioma cells were validated by the model The observation that δz was a significant parameter in all cases, and that the magnitude and direction of this effect varied according ... effect, but the method has limitations Results Firstly, the fitting of these models has always been performed separately on the treated and untreated datasets, making direct comparison ofthe parameters...
... prior to UV-< /b> B irradiation of and 2.5 µM DCFH-DA was added to the treated cultures The ROS produced was measured as above the enzyme was estimated to be approximately 49 kDa The 49-kDa MBPK activity ... responses Both the treatments blocked the UV-< /b> B- induced stimulation of MBPK and CDPK activities and the UV-< /b> Binduced accumulation of Tdc and Str mRNAs, and catharanthine Because EGTA and verapamil are ... CDPK Then, the activated CDPK would regulate the activation of NADPH oxidase in the plasma membrane and release ROS Finally, downstream of ROS production, the UV-< /b> Binduced and -activated MAP kinases...
... effect of adsorption of TCE on the TiO2 powder), as well asthe photolysis of TCE by solar radiation only in the absence of TiO2 Figure showed the degradation ratio of TCE asthefunctionof exposed ... the byproducts from the TCE degradation were examined MATERIALS AND METHODS TCE (99+%) was obtained from Aldrich Chemical Co, and the TiO2 used was Degussa P-25, which was mostly anatase and had ... Operation ofa solar photocatalytic water: Treatment system at a superfund site Photocatalytic Purification and Treatment of Water and Air, Edited by D F Otlis and H Al-Ekabi, pp 547-556 Raquel...
... decreases the enthalpy ofthe main phase transition of Myr2Gro-PGro substantially whereas GS12 and GS10 actually appear to slightly increase the total enthalpy ofthe two-component main phase transition ... gel phase with tilted hydrocarbon chains (the Lb¢ phase) to the rippled gel phase with tilted hydrocarbon chains (the Pb¢ phase) and the main phase transition to the conversion ofthe Pb¢ phase ... antimicrobial potency of these analogs and possibly also for understanding the molecular basis for their differential antimicrobial potencies again different classes and species of bacteria, many of...
... affinity, K, can be derived for both thea and b subunits from the averaged parameters of HbA oxygenation (Table 1, Average) The association and dissociation rate constants for theb subunits are found ... yield of BR By contrast, the decrease in the association rate constant for theb subunits occurs with the increase in the quantum yield of BR The results for theb subunits show that there is an ... of Val(E11) in thea and b subunits relative to the heme plane is quite dependent on the nature ofthe anions and the pD ofthe solution as well as on the nature ofthe ligand It has been observed...
... City, CA, USA) 360 CAGCTTCCTCGTATCTACATTCAAGAGATG TAGATACGAGGAAGCTGTTTTT; 1179 CCATTCTGCCGTATATTGATTCAAGAGA TCAATATACGGCAGAATGGTTTTT; 1624 CTCAGAGATTGCCCTTTCTTTCAAGAGA AGAAAGGGCAATCTCTGAGTTTTT The ... (F) As an additional proof of apoptosis, cleaved caspase-3 was determined in cell extracts by western blot (G) Representative blot of n = experiments showing 17 and 19 kDa caspase-3 cleavage bands ... TO H Actin siR N A J H Lee et al Caspase Caspase Cleaved caspase Cleaved caspase Fig Effect of PHB siRNA on ethanolinduced apoptosis in RINm5F and INS-1E cells RINm5F (A, C,E) and INS-1E (B, D)...
... NM_021283, CgAggTCACAggAgAAgggA AAgCCCTACAgACgAgCTCACT Actin NM_007393.3 CAACgAgCggTTCCgATg gCCACAggATTCCATACCCA Rn18s NR_003286.2 gTAACCCgTTgAACCCCATT CCATCCAATCggTAgTAgCg Straccia et al Journal of Neuroinflammation ... gTttCCAATcagccc -16/-31 -173/-188 TCAggAACAgTTgCCATAgC AgACCTATACAACggCTCCT gTTgTgATTCTTTCgATgCT ggAATTgACTATCgTTCTTg TNFa agggTTtgGaAAgtt -336/-351 TCTCATTCAACCCTCggAAA CACACACACCCTCCTgATTg IL10 aggATTGaGaAATaa ... were added and the mixture was incubated at 40 rpm on a rotating wheel for at least h at 4°C Then, the tube was placed on a magnetic rack for The supernatant was discarded and 100 μL of sheared...
... each well The QuantiChromeTM Alkaline Phosphatase Assay Kit (Cat No DALP-250; BioAssay Systems, Hayward, CA) was used to measure alkaline phosphatase (AP) activity levels in lysate samples of 104 ... included a DNase treatment using the RNase free DNase Set (Qiagen, Hilden, Germany) The yield and purity ofthe RNA was measured photometrically RNA was analyzed by quantitative real time polymerase ... osteoclast progenitors, and leads to osteoclast activation On the other hand, OPG binds to RANKL and thereby inhibits osteoclast activation The osteoblast function can be described by alkaline...
... each well The QuantiChromeTM Alkaline Phosphatase Assay Kit (Cat No DALP-250; BioAssay Systems, Hayward, CA) was used to measure alkaline phosphatase (AP) activity levels in lysate samples of 104 ... included a DNase treatment using the RNase free DNase Set (Qiagen, Hilden, Germany) The yield and purity ofthe RNA was measured photometrically RNA was analyzed by quantitative real time polymerase ... osteoclast progenitors, and leads to osteoclast activation On the other hand, OPG binds to RANKL and thereby inhibits osteoclast activation The osteoblast function can be described by alkaline...
... peroxyl radical; (a) decay curves of absorbance at 460 nm (Abs460) and (b) plots of ln (Abs0/Abst) versus time in the presence of antioxidants (10 lM), where Abs0 is initial Abs460 and Abst is Abs460 ... pseudofirst-order rate constants kobs of b- carotene bleaching with linoleic acid peroxyl radical at 50°C Values of kobs were obtained by monitoring the absorbance of b- carotene aqueous solution (8.2 lM) at 460 ... evaluating antioxidant activity The method is based on the discoloration ofthe yellowish color ofa b- carotene solution due to the breaking of p-conjugation by the addition of lipid peroxyl radical...
... lumbar vertebrae Abnormal arrangement of lumbar vertebrae Fusion of thoracic vertebrae Absence of ribs Fusion of ribs Bifurcation of ribs Shortening of ribs Displasia of sternebrae Missing of ... from the tip ofthe snout to the base ofthe tail The longitudinal distance from the tip ofthe snout to the base ofthe skull was recorded as head length The distance between the two ears was ... Internal malformation Fetuses examined Dilatation of cerebral ventricle Stenosis of nasal cavity Cleft palate Dextrocardia Levorotation of heart Abnormal lobation of lung Detect of diaphragm Diaphragmatic...
... vertebrae Fusion of thoracic vertebrae Absence of ribs Fusion of ribs Wavy ribs Hypoplasia of ribs Displasia of sternebrae Missing of sternebrae Hypoplasia of sternebrae Curvature of tibia Absence ... ventricle, dilatation of renal pelvis and abnormalities ofthe extremities and tail With increasing radiation dose, cleft palate, dilatation of cerebral ventricle and abnormalities ofthe extremities ... distance from the tip ofthe snout to the base ofthe skull was recorded as head length The distance between the two ears was recorded as head width Measurements were made with a vernier callipers...
... ErPC3; PARP-cleavage was accompanied by a weak activation of caspase-3 detectable upon treatment with 12.5 µM ErPC3 (A) A weak cleavage of caspase-3 and PARP was also observed when LNCaP cells ... Germany) Rabbit antibodies against PARP, caspase-3, Akt, phospho-Akt (Ser473), Bax, Mcl-1, and Bcl-xL were purchased from Cell Signaling (Frankfurt, Germany), the rabbit anti-Bak NT antibody was from ... ErPC3, as indicated (D-F) 48 h after treatment a WST-1 Assay was performed The absorption correlates with the number of viable cells and was normalized to that of untreated controls PC3 (A) and...
... were analysed using a time-resolved immunofluorometric assay, with the MAb 6E8 asa capture antibody for TATI and a europium (Eu) labelled antibody 1 1B3 asa tracer [2,15] Fluorescence was measured ... s-TATI concentrations increased considerably after surgery for many ofthe short-term regimen treated patients, which supports the theory that TATI can behave as an acute phase reactant, as demonstrated ... demonstrate that concentrations of TATI in tumour tissue or serum are not affected by neoadjuvant radiotherapy in rectal cancer patients The finding of an association between both t-TATI and sTATI,...
... rituximab-treated patients as compared with rituximab-naïve The absolute numbers ofB cells in the rituximab-treated and rituximab-naïve patients are shown in Table The accumulation of immature subset ... Rituximab was provided intravenously in two doses of 1000 mg each on days and 15 The efficacy of rituximab treatment was assessed clinically by disease activity score (DAS) 28, a composite measure based ... in BM, and by an increase of immature (CD27-IgD) B cells (P = 0.01) The absolute numbers ofB cells in the rituximab-treated and tituximab-naïve patients are shown in Table No correlation was...
... perspective on garlic and cardiovascular disease J Nutr 2001, 131:977S-979S Tanaka S, Haruma K, Yoshihara M, Kajiyama G, Kira K, Amagase H, Chayama K: Aged garlic extract has potential suppressive ... Okamoto H, Iwamoto T, Kotake S, Momohara S, Yamanaka H, Kamatani N: Inhibition of NF-kappaB signaling by fenofibrate, a peroxisome proliferator-activated receptor-alpha ligand, presents a therapeutic ... NF- B was confirmed by competition assays as well as by super shift assays In the presence ofa p50 antibody, the DNA-binding activities of NF B showed a super shift However, in the presence of a...
... were analyzed with a FACSCalibur (BD Bioscience, San Jose, CA, USA) Migration assay Cell migration was assessed in 24-well chemotaxis chambers (6.5 mm diameter, μm pore polycarbonate transwell ... UK) was added and theB cells were incubated for 24 hours Afterward, the incorporated radioactivity was quantified After the 72-hour incubation, viabilities ofthe cells, determined by trypan blue ... were analyzed with a FACSCaliber Statistical analysis Paired t test was used to compare paired samples of CD27and CD27+ peripheral blood B cells, and peripheral blood and synovial B cells from the...