use of hemocytometer for determining total cell no in liquid susp
modern neuroscience research protocol
... started hardening tissue in alcohol (spirits of wine), cutting sections with his home-made microtome and staining them with various coloring agents including indigo, tincture of iodine and chrome ... detector for biotin in both light and electron microscopy This in turn allows biotin to be used as a single -cell marker, generally in the form of one of its derivatives such as Nε-biotinyl-L-lysine ... what is missing' is likely to depend on the questions being asked Certainly no Cytological Staining Methods Fig Examples of staining of single neurons by intracellular iontophoresis of horseradish...
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Báo cáo hóa học: " A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus" pdf
... tool for the subtyping all NA subtypes of influenza A viruses from a range of Mammalian and Avian species The sequence information obtained can be helpful in determining the origin of the influenza ... sequences for each subtype The standard mixed base definition was applied, and for reference "I" stands for inosine aAlignment is presented in sequence logo format [32-34] An advantage of subtyping influenza ... given for the total population of samples The standard mixed base definition was applied, and for reference "I" stands for inosine aAlignment is presented in sequence logo format [32-34] Another...
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Primer design for the PCR amplification of the coad gene
... glutamate instead of glutamine) An alternative strategy which will result in the amplification of the original gene is described in Utilisation of the Nco I site 3'-end primer No C-terminal tag is being ... However, PCR using this 5'-end primer will not result in the amplification of the original coaD gene The use of the Nco I restriction site dictates what the first nucleotide of the next triplet ... must be (G) In the coaD gene, however, the second triplet begins with a C and PCR amplification will change this codon from CAA to GAA (and the second residue in the recombinant protein will be...
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Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt
... specificity of the dengue primers was tested against 291 nondengue flaviviruses, including 67 strains of Japanese encephalitis virus, 28 strains of St Louis encephalitis virus, 172 strains of West ... phenol ⁄ chloroform extraction to obtain total RNA from both infected and control normal cells Total RNA was used as the template for real-time RT-PCR Dengue virus cDNA and RNA were tested in ... DENV-1 strains in the 163-strain design basis set, and no strain of any other serotype By selecting one primer pair from each group, nearly any of the 163 design-basis dengue strains may in principle...
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Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
... 436980 34114 Presence of NlaIII site Presence of oligo(dT) 89 211 217 189 192 254 97 232 94 101 198 165 182 184 274 317 123 Tag No No No No No No No No No No No No No No No No No Yes Yes Yes Yes ... requires more initial total RNA and poly(A)+ than SAGE, because of the loss of RNA in each step Thus, the demand for RNA restricts the application of this method during the low total RNA experiment, ... improves genome annotation: identifications of novel genes and alternative transcripts in the mouse Bioinformatics 21, 1389–1392 Saha S, Sparks AB, Rago C, Akmaev V, Wang CJ, Vogelstein B, Kinzler...
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pcr detection of microbial pathogens
... Performance of Amplification Assays When using a PCR assay for clinical samples containing only a few cells of the pathogen, one has to be aware of the special kinetic conditions prevailing in ... Relief of inhibition by meat, blood, and feces Relief of inhibition by bilirubin and humics Relief of inhibition by melanin in RT-PCR Relief of inhibition by meat, blood, and feces Relief of inhibition ... hemoglobin and lactoferrin contain iron The mechanism of inhibition may be related to the ability of these proteins to release iron ions into the PCR mixture When the inhibitory effect of iron was investigated,...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... 4:130 strain of virus and the cell type infected Thus it is not possible to identify a single housekeeping gene for use in QPCR studies of viral infections Nevertheless it is of benefit for researchers ... by determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines ... of differences in the virus strain used and a very similar pattern was also seen in VZVSchenke infected cells despite differences in the origin of the primary cell types used for infection Though...
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Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
... boundaries indicate the 5' ends of the innermost amplification primers against the genome of strain J4 been developed to prevent HCV infection The best available therapy for HCV infection is a combination ... introduced into the template DNAs during RT-PCR However, determining consensus sequence by directly sequencing uncloned templates greatly reduces the impact of this type of error because for an enzymatically-derived ... source of error in sequencing is due to primer bias Primer bias is selective amplification of a portion of the sequences in the target population and is a result of varying primer affinities for...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... 4:130 strain of virus and the cell type infected Thus it is not possible to identify a single housekeeping gene for use in QPCR studies of viral infections Nevertheless it is of benefit for researchers ... by determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines ... of differences in the virus strain used and a very similar pattern was also seen in VZVSchenke infected cells despite differences in the origin of the primary cell types used for infection Though...
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báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
... boundaries indicate the 5' ends of the innermost amplification primers against the genome of strain J4 been developed to prevent HCV infection The best available therapy for HCV infection is a combination ... introduced into the template DNAs during RT-PCR However, determining consensus sequence by directly sequencing uncloned templates greatly reduces the impact of this type of error because for an enzymatically-derived ... source of error in sequencing is due to primer bias Primer bias is selective amplification of a portion of the sequences in the target population and is a result of varying primer affinities for...
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Báo cáo y học: "Selective amplification of glucocorticoid anti-inflammatory activity through synergistic multi-target action of a combination drug" ppsx
... adjuvant-induced model of arthritis Interestingly, the effect of combination was most pronounced in the phalangeal joints, where clinically relevant suppression of histologic markers of inflammation, ... profile for this combination, in which dipyridamole amplifies the desired anti-inflammatory activity of prednisolone in immune cells without enhancing glucocorticoid action in alternative cellular ... dipyridamole in CIA models in Lewis and Louvain rats To test the activity of the combination in this model of chronic inflammation, rats were sensitized with colla- Page of 14 (page number not for citation...
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Báo cáo lâm nghiệp: "Amplification of root—fungus interface in ectomycorrhizae by Hartig net architecture" pot
... dissertation, Tubingen F.R.G Kottke I & C!berwinkler F (1987) Cellular structure and function of the Hartig net: coenocytic and transfer cell- like organization Nord J Bot 7,135-95 Mangin L (19’10) Introduction ... the a The intimate contact with the cell surface and the transfer cell- like structure of the Hartig net must therefore be considered as not only promoting absorption by but also releasing solutes ... models of area and perimeter of hyphal complex and length of hyphal walls are presented in Table I (Trinci, 1984; Prosser, 1983) The result is pinnate growth of scattered hyphae, spreading quickly...
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Báo cáo y học: "Amplification of autoimmune disease by infection" potx
... present in many sera and can be administered therapeutically in the form of intravenous immunoglobulin to immunodeficient patients [66] It is not known whether this virus uses hematopoietic cells for ... by the infection Our data from immune-suppressed mice showed extensive viral infection, with MHV-68 in the synovium involving all cell types including fibroblasts and synovial lining cells [4] ... CCchemokine gene, MCK-2 The presence of MCK-2 results in greater inflammatory responses and enables CMV shedding in the salivary glands [40,41] HHV6 infects cells of the myelomonocytic lineage...
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Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps
... http://www.wjso.com/content/6/1/56 were incubated in the dark for minutes Sections were then counterstained in Mayer's haematoxylin and dehydrated in ascending grades of methanol before clearing in xylene and mounting under ... was no significant relationship of E-cadherin, α-catenin, β-catenin and γ-catenin in breast tumours to Nottingham Prognostic Index (NPI) (E-cadherin p = 0.094, αcatenin p = 0.144, β-catenin p ... staining as a separate category Thus, it is possible that lobular carcinoma cases with cytoplasmic staining were included in the general category of reduced expression of E-cadherin staining...
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Báo cáo y học: "Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries" pps
... (NEB) and were incubated stepwise for 15 minutes at 12°C and 15 minutes at 25°C The non-templated 3’-single-dA extension was performed for 30 minutes at 37°C in 50 μl containing 1× Klenow buffer ... machine performance by adjusting the temperature ramp rates of different types of instruments We therefore optimized the reaction conditions on the PCR machine with the fastest heating and cooling ... mean normalized coverage of 50-bp windows in the human genome having the GC-content indicated on the x-axis for a PCR-free (orange dots) and a PCR-amplified (blue diamonds) Illumina sequencing...
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Báo cáo y học: " Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR" doc
... the patients as a whole instead of dividing them into subgroups according to their primary diseases, because we aimed to define a routine test competent enough for most, if not all, patients Actually, ... that PCR using PBL DNA is not suitable for diagnosing active CMV infection, because healthy adults with the latent infection may harbor CMV in their leukocytes Nevertheless, for immunosuppression ... suitable for small size of samples demanding highly sensitive detection, such as: (1) monitoring HCMV infection in immunocompromised patients; (2) diagnosing congenital or prenatal infection of HCMV,...
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Báo cáo khoa học: " Nested-multiplex PCR detection of Orthopoxvirus and Parapoxvirus directly from exanthematic clinical samples" pdf
... of viruses included in these genera directly from bovine, ovine, caprine and human exanthematic lesions with no viral isolation or DNA manipulation Sixty-eight clinical samples collected during ... performed with a DNA Mastercycler Epgradient (Eppendorf, Hamburg, Germany) using the following protocol: incubation at 95°C for min; 30 cycles of denaturation (94°C, min), annealing (45°C, min) ... Outbreak of exantemal disease caused by Vaccinia virus in human and cattle in Zona da Mata region, Minas Gerais Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 2005, 57:423-429 Inoshima...
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Báo cáo sinh học: "Rapid PCR detection of group a streptococcus from flocked throat swabs: A retrospective clinical study" ppsx
... reactions were performed in 10 μL volume, including μL of Light Scanner master mix containing the LCGreen Plus + dye (Idaho Technology Inc., Salt Lake City, UT), a 0.5 μmol/L concentration of each primer, ... rate of 9.9°C per second, with a total run time of 35 minutes After PCR, samples were transferred to the high-resolution melting instrument (HR-1) A rate of 0.3°C per second was used when measuring ... Slinger et al Annals of Clinical Microbiology and Antimicrobials 2011, 10:33 http://www.ann-clinmicrob.com/content/10/1/33 then heated at 100°C for 10 minutes, and then centrifuged again for minutes...
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Báo cáo y học: "Serologic and PCR testing of persons with chronic fatigue syndrome in the United States shows no association with xenotropic or polytropic murine leukemia virus-related viruses" pot
... and xenotropic MuLV’s are capable of infecting non-murine cells, other controls will need to be developed to rule out contamination from non-murine sources Page of In conclusion, we have used ... 22Rv1 cell line, concluding that any discovery of these conserved XMRV sequences in patient samples was due to contamination [23] Given the high degree of known risk for contamination even in laboratories ... carcinoma cells were used as positive controls in this test [15] 1.0 μg of DNA (333 ng of PBMC DNA) was used in the nested pol and gag PCR tests at the CDC for which 1,000 and 10 copies of the...
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Báo cáo sinh học: "Long telomeres in the polytene chromosomes of Drosophila melanogaster are associated with amplification of subtelomeric repeat sequences" doc
... sublines were maintained at 23°C The telomere morphology of the sublines starting examined in polytene chromosome squashes prepared (Atherton and Gall, 1972) with minor modifications was In situ ... Six sublines differing in telomere morphology with particular combinations of long, intermediate, and short telomeres are diagrammed in figure (lines A-F) The telomeres in some lines are not as ... (Kurenova, personal communication) The Drosophila melanogaster strain T-32, originally selected for increased heat tolerance, has provided an excellent experimental model for obtaining sublines...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25