use climate and hydrological change in the catchment

... extensive remodeling, suggesting the intimate interactions between the various membrane components Interest towards the understanding how lipids and their interacting partners function in such a systems ... tri), x1 and x2, the length of the sphingoid base and fatty acyl chain respectively, y1 and y2, the number of double bonds on the sphingoid base and fatty acyl chain respectively, and Z the lipid ... changing their sphingolipid composition The interactions between sterols and sphingolipids were further probed genetically by combining mutations in sterol biosynthesis with mutants in sphingolipid...

Ngày tải lên: 14/09/2015, 14:06

... with orthologs in other organisms They are the two isoforms of the ADP ⁄ ATP translocase [14–16], the carnitine ⁄ acylcarnitine [17,18], citrate [13] and mitoferrin carriers [19] In the present ... serum albumin, ovalbumin, carbonic anhydrase, trypsin inhibitor and lysozyme); lanes 1–4, E coli BL21(DE3) containing the expression vector, without (lanes and 3) and with the coding sequence ... electrophoresis Band intensities Reconstitution into liposomes and transport assays The recombinant protein in sarkosyl was reconstituted into liposomes in the presence or absence of substrates [23] The reconstitution...

Ngày tải lên: 29/03/2014, 08:20

... development was the other essential part The limiting parameter has become not the machine but the ability to analyze the reams of data that we get What was your initial reaction to the results, and how ... immersed in the life-sciences community and has a long-standing interest in SIMS instrumental developments Slodzian is an ion physicist of enormous skill and reputation who has been responsible for the ... were in place, the remaining challenge was developing the functional software and computational know-how to analyze all the data Each image pixel has an intensity that is a function of the number...

Ngày tải lên: 06/08/2014, 18:21

... hormone is the dominating hormone in ruminants (Flint and Knight, 1997); it increases the blood flow in mammary glands and has blood glucose-elevating effects Prolactin increases the intestinal uptake ... AdipoQ binds to the N-terminal extracellular domain, whereas the intracellular C terminal domain binds to APPL1 (an adaptor protein containing a pleckstrin homology domain, a phosphotyrosine binding ... 3: Adiponectin receptors and signaling AdipoR1/R2 = adiponectin receptor and 2, APPL1 = adaptor protein containing pleckstrin homology domain, phosphotyrosine binding domain and leucine zipper...

Ngày tải lên: 20/11/2015, 15:49

... disrupted, and this gives rise to an increase in Km Also, the polarity is changed, and the increase in the size of the side chain may create steric hindrance for the substrate, making the base ... because ATP is saturating in our experiments, when evaluating the impact of the mutations from the kinetic data, a change in the Km value can be interpreted as an effect on substrate binding, and ... between base and amino acid is the main reason for the increase in Km values The point mutation probably does not alter the backbone conformation of the Ca-atoms In conclusion, the kinetic and structural...

Ngày tải lên: 19/02/2014, 02:20

... 3¢-azido-2¢,3¢-dideoxythymidine), zalcitabine (ddC, 2¢,3¢-dideoxycytidine) and brivudin [BVDU, (E)-5-(2-bromovinyl)-2¢-deoxyuridine] The kinetic parameters for these are given in Table When discussing the binding and the ... space as the base in the dCTP and dTTP ligands (Fig 6) The guanosine base is in the syn-conformation, in contrast to the thymine and cytosine bases that are in the anticonformation in those complexes ... Surprisingly, there were two sulfate ions present – one bound in the P loop, as observed in the other substrate complexes, and the other located between the first sulfate ion and the substrate There...

Ngày tải lên: 16/03/2014, 06:20

... diplomacy proved wholly ineffective in promoting dialogue between Kabila and rebels based in Uganda and Rwanda, and in preventing Angola, Namibia and Zimbabwe from sending in troops to prop up his ... multilateralism as the entry point of achieving these objectives: strengthening the African Union (AU) and the Southern African Development Community (SADC) and supporting the implementation of the New ... drawing the usual criticism of meddling in the internal affairs of other states Therefore, building multilateral capacity has been accompanied by assembling and training soldiers as well as the...

Ngày tải lên: 29/03/2014, 03:20

... from proliferating and floating spherical colonies have been shown to incorporate bromodesoyuridine into the DNA indicating their proliferation ability In addition, they retain the ability to ... poly-D-lysine (Sigma) and μg/mL laminin (Sigma) After plating, dishes were maintained for two hours, at 37°C and 5% CO 2, and centrifuged at 200 × g, at 4°C, for two minutes[20] The remaining medium ... cells in the postnatal guinea pig, retaining an undifferentiated phenotype, as observed in the mouse Our results clearly show the staining for protein markers for both hair cells and supporting cells...

Ngày tải lên: 18/06/2014, 16:20

... negative one, and all the points involving a-Gpdh For s z lAdh F Est-6/-, the gametes carrying the Est-6allele and the Est-6 and Est-6 s la-Gpdh F gametes are in the positive direction of the first ... from each individual In our case the individuals are the populations, and the variables are the genotypic and gametic frequencies II Material and methods Two wine cellar populations and two field ... axis, and the alternative homozygotes are in the negative direction This means that FF changes in Adh homozygote frequencies are in the same direction as changes in SS homozygote frequencies for the...

Ngày tải lên: 09/08/2014, 22:22

... more, in that they had on hand nothing but sixty and ninety day paper They would willingly have paid a higher rate for the credit they needed On the other hand, in maintaining discount a t the ... soda, the improvements in the paper industry, and, finally, mechanical inventions for spinning and weaving-all these were the vanguard of the one supreme invention most marvelous of all, namely, the ... another The financial agents of the State could not take these notes, since they were not current in Paris; they could not send them to the Minister of Finance The Bank of France, on the other...

Ngày tải lên: 05/11/2014, 08:18

... the first corresponding to the MP analysis and the second (in brackets) to the ML analysis The Forsythia europaea and Olea woodiana sequences were used as outgroups The tree was rooted with the ... (52,165 and 83,304) are polymorphic in both clades Cp-I and Cp-II, suggesting that these sites could be under selective pressures, either maintaining polymorphism or contributing to the recurrent ... detected in wild olives than in cultivars, and this allowed some population genetic analyses, for instance in the laperrinei and guanchica subspecies from Saharan Mountains and Canary Islands, respectively...

Ngày tải lên: 11/08/2014, 11:20

... theophylline may be through the cAMP pathway and further inhibits the expression of LTC4 and LTD4 Conclusion These data may facilitate the understanding of the diverse anti-inflammatory effects of theophylline, ... expressed genes in macrophages after incubating with theophylline Our study confirmed the diverse roles of theophylline as an immune modulator, which may be helpful in improving its use in the treatment ... demonstrated that theophylline has a steroid-sparing effect [17,18] Theophylline inhibits the degranulation and release of mediators, including platelet-activating factor, LTC4, cationic proteins, and superoxide...

Ngày tải lên: 12/08/2014, 18:22

... C2-like domain (III) and a calmodulin-like calcium binding domain (IV) The main difference between mammalian and Drosophila calpains is represented by the length of the N-terminal domains; for example, ... recombinant calpain B was used as a control (C) The mass of the standards (St) is given in kDa on the left-hand side The excessive band at around 52 kDa indicates the presence of immunoglobulins ... generated (aiming to avoid unwanted autoproteolytic degradation during sample handling) by replacing Cys314 with Ala in the active center of the enzyme The active calpain B and the inactive C314A...

Ngày tải lên: 16/03/2014, 02:20

... removes the N-terminal dipeptide, and because it is inhibited by the specific DPP III inhibitor tynorphin [18] The Km values of the recombinant D melanogaster DPP III for proctolin (4 lM and lM) ... RT-PCR using RNA from transfected S2 cells after induction (lane 5) After a 5-min incubation of proctolin (25 nmol) with stably transfected and induced S2 cells in NaCl/Pi containing the aminopeptidase ... DPP III This fragment was inserted in the pMTA/V5-His B expression vector Arrows represent the primer pairs (1 and 2; and 4) used in the PCR and RT-PCR experiments, and the approximative sequence...

Ngày tải lên: 17/03/2014, 10:20

... and similar amino acid residues are boxed in black and gray, respectively The interdomain connecting loop and the C-terminal tail, known to be important for interaction of PCNA-binding proteins, ... (A) In vitro interaction of DmPCNA2 and DmPCNA1 Lanes 1–5: the indicated proteins were mixed in NaCl ⁄ Pi at °C for 12 h Lanes 6–10: the indicated proteins were mixed in NaCl ⁄ Pi containing ... nonchromatin-bound protein) and histone H4 (a chromatin-bound protein) DmPCNA2 and DmPCNA1 were present in the cytoplasmic and the whole chromatin fractions (Fig 6B) Following exposure to DNA-damaging...

Ngày tải lên: 23/03/2014, 10:20

... However, controlling the environment within these joints will be of the utmost importance in ensuring the success of ACT in OA patients Hopefully, the development of novel tissue engineering strategies, ... Fibronectin fragments and blocking antibodies to alpha2beta1 and alpha5beta1 integrins stimulate mitogen-activated protein kinase signaling and increase collagenase (matrix metalloproteinase 13) ... manufacturing engineering practice guidelines required for tissue Gene expression analysis RNA was extracted and isolated from chondrocytes using the RNeasy mini kit according to the manufacturer's instructions...

Ngày tải lên: 09/08/2014, 10:20

... the interview If the error is detected again in following interviewers, the interviewer is removed from the study Use of highly structured CATI interviews with well-trained carefully monitored interviewers ... 96-spin blood protocol The protocol entails adding protease, the sample, and lysis buffer to 96well plates The plates are then mixed and incubated at 70°C, before adding ethanol and transferring the ... clinician-administered interview; the cost per lay-administered interview from SRBI is approximately $50 per interview The use of clinician-administered interview would have mean greatly reducing...

Ngày tải lên: 11/08/2014, 17:20

... while in the fixative Following a prolonged rinse in the same buffer, the ovarian follicles were postfixed for h in 1% Osmium tetroxide in 0.1 M cacodylate buffer at pH 7.2 and rinsed again in the ... detected in a U-line bearing or not the fs(2)A17 mutation Gold grains were counted in the follicle cells and the oocyte comprised within a 0.8 × 1.6 µm reptangular frame bridging the perivitelline ... precipitate in the intercellular spaces of the follicular epithelium, giving rise to such abnormal structures as globules and crystalline fibers [14] Since the f(1)1163 mutation and the genetic determinant...

Ngày tải lên: 13/08/2014, 09:21

... during varying time periods that I am focussing on in this thesis (Mautner 2008) A stringent and meticulous process was involved in the collecting, collating, organizing, coding and analyzing ... periods: the 1960s and 1970s, the 1980s and 1990s, and the 2000s After collecting, 20 collating and organizing the data as such, I scrutinized and coded the data to identify the discourses involved ... heights and the economic life of all the islands, with the exception of Java, was focused upon Singapore” (Tregonning 1972:87) In order to meet the increasing demand of the growing population, the...

Ngày tải lên: 03/10/2015, 20:30