urinary proteomics and candidate biomarker discovery for diabetic nephropathy
Báo cáo y học: "Proliferation and differentiation potential of chondrocytes from osteoarthritic patients" ppt
... cartilage and subchondral bone and to withstand the mechanical forces applied within the joint R567 The results in this paper give hopes for finding a cell-based autologous biological treatment for ... experiments, performed immunohistochemical staining, performed the statistical analysis and drafted the manuscript C Bengtsson performed the cell culture and RNA preparations C Brantsing performed immunohistochemical ... too far and that the implant would therefore be less integrative In contrast, in the treatment of large injuries, the scaffold needs to be able to withstand mechanical load and shear forces from...
- 9
- 416
- 0
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 2
... mixed and left to stand at room temperature for 5-10 minutes The organic and aqueous phases were separated by adding 240μl of chloroform and 240 μl of 2N KCl The mixture was then vortexed, and ... were then evaluated for their efficiency and specificity to inhibit human exogenous and endogenous SPHK; the compounds were also tested for their potential cytotoxicity effects, and counter screened ... DAGK and PKC for their specificity The most widely used SPHK inhibitor DMS, also inhibits DAGK and some PKC isoforms, suggesting that any novel SPHK inhibitors may possibly also inhibit DAGK and...
- 32
- 310
- 0
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 3
... was used for master mix of PCR reagents (QIAGEN, #204143) Thermal cycling conditions for PCR and dissociation routines were: 95°C for 10 minutes, 45 cycles of 94°C for 30 seconds, 60°C for 45 seconds, ... of DMS and CP6 on Human BM- and AD- MSCs Growth CP6 and DMS were first tested at different concentrations for their potential cytotoxic and/ or anti-proliferative effects Six doses of DMS and CP6 ... This is a novel method using linear double-stranded DNA molecules as the standards instead of using the typical gene-in-plasmid format Standards for different genes were obtained from the serial...
- 58
- 249
- 0
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4
... trypsin and washed once in 2% FCS/PBS before counting Cells were rested for 40 minutes in maintenance media before 1x105 cells were then aliquoted into a 96-well plate and pelleted at 450 x g for ... subsequently added and cells were incubated on ice for 20 min, followed by two washes in 2% FCS/PBS, before resuspension in 100 μl of 4% paraformaldehyde and incubation on ice for 20 Cells were ... human BM- and AD- MSCs Total RNA was extracted from both types of cells, and cDNAs for S1PR1, S1PR2, and S1PR3 were prepared as serial controls for real time PCR Serial controls of S1PR4 and S1PR5...
- 31
- 246
- 0
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 5
... understanding of human MSCs osteogenic/adipogenic differentiation The data described in chapter might also pave the way, for a more controlled and facilitate differentiation of stem cells, for future ... Therefore, more work need to be done to further confirm our conclusions Finally, the study could be expanded to study other stem cells and other differentiations pathways, to further validate and ... amounts (1μM DMS and 5μM CP6) showed to inhibit the osteogenic differentiation in most of the experiments Interestingly, in human BM- and AD- MSCs adipogenic differentiation, DMS and CP6 showed...
- 5
- 268
- 0
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6
... (1997) "Evidence for two psoriasis susceptibility loci (HLA and 17q) and two novel candidate regions (16q and 20p) by genome-wide scan." Hum Mol Genet 6(8): 1349-56 Nirmalanandhan, V S., M Rao, ... 590-2 Safford, K M and H E Rice (2005) "Stem cell therapy for neurologic disorders: therapeutic potential of adipose-derived stem cells." Curr Drug Targets 6(1): 57-62 Safford, K M., S D Safford, ... mesenchymal stem cells and development of an immunocompetent mouse model for ex vivo osteoporosis gene therapy." Hum Gene Ther 15(12): 1197-206 Kundu, A K and A J Putnam (2006) "Vitronectin and collagen...
- 25
- 467
- 0
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation
... two isoforms may have distinct cellular functions and may be regulated by different signalling mechanisms Unfortunately, so far, no 3-dimention structure information is available for SPHK1 and SPHK2, ... to Gi; S1PR2 and S1PR3 activate Gi, Gq and G12/13; and S1PR5 is coupled to Gi and G12/13 (Spiegel and Milstien, 2003) Among all five S1PRs, S1PR1, S1PR2 and S1PR3 are most widely expressed, while ... one hand, it could act as an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and promote cell survival and cell growth On the other hand,...
- 31
- 524
- 0
Control of Cell Proliferation and Growth byMyc Proteins
... adhesion to the basal lamina and Myc disrupts this adhesion; therefore, keratinocytes respond to Myc with premature differentiation and arrest of proliferation (Gandarillas and Watt 1997) The frequent ... p53 and ARF and therefore bypass a mutational requirement that exist for wild-type Myc Does that mean that Fbw7γ is a co-factor of repression? Possibly the situation is very similar to that for ... oxygen (Soucek and Evan 2002) This is formally possible: however, several Myc-induced tumors are genomically unstable and show complex karyotypes and enhanced rates of double-strand breaks, which...
- 14
- 376
- 0
Tài liệu Báo cáo khoa học: a-Defensins increase lung fibroblast proliferation and collagen synthesis via the b-catenin signaling pathway doc
... a-defensin-1 and a-defensin-2 increases collagen synthesis, HFL-1 lung fibroblasts were incubated with a-defensin-1 and a-defensin-2 (0.5–6 lm) for 24 h and then collagen protein content and the collagen-I ... kinase and PI3K ⁄ Akt and increase phosphorylation of GSK3b on Thr390 and Ser9 SB203580 and LY294002 prevented a-defensin-induced increases in the protein contents of Thr390-phosphorylated and Ser9-phosphorylated ... PCR was performed using ABI 7500 Sequence Detector (Perkin-Elmer Applied Biosystem, Foster City, CA, USA) with the conditions: 95 °C for 10 min, 40 cycles of 95 °C for 15 s and 60 °C for All the...
- 12
- 602
- 0
Cytoskeleton reorganization mediates alpha beta integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells pdf
... Agricultural and Scientific Research agencies (approval number A 37801) and conducted in accordance with the guidelines for Care and Use of Agricultural Animals in Agricultural Research and Teaching ... pg/well) and the intra- and inter-assay coefficients of variation were less than 7% and 9% respectively The progesterone detection limit was 12 pg/tube (60 pg/ well), and the intra- and inter-assay ... staining and fluorescence microscopy After culture in chamber slides, GC were fixed for 15 with 4% paraformaldehyde in PBS Cells were then washed with PBS and left in 0.1 M glycine in PBS for 15...
- 17
- 521
- 0
Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx
... estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsible for converting androgen ... papillomas and carcinomas by dehydroepiandrosterone and 3-b-methylandrost-5-en-17-one in mice Cancer Res 45, 164–166 Schwartz A & Pashko L (1995) Cancer prevention with dehydroepiandrosterone and non-androgenic ... assess this, antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and an inhibitor of the aromatase responsible for conversion of androgen to estrogen (letrozol),...
- 12
- 534
- 0
Báo cáo khoa học: G protein-coupled receptor-induced Akt activity in cellular proliferation and apoptosis pptx
... which are probably stimulus- and ⁄ or cell type-specific This stabilizes the active conformation of Akt and allows it to translocate to the cytoplasm or nucleus to search for its many target proteins ... through the direct and indirect phosphorylation of a number of cell cycle regulatory proteins [11], and its ability to inactivate pro-apoptotic factors, such as Bad, caspase-9 and forkhead (FH) transcription ... be transactivated by GPCRs through Ga- or Gbc-dependent pathways [22,23] For example, ligands for the LPA, endothelin-1 and thrombin receptors all promote DNA synthesis in Rat1 fibroblasts by transactivating...
- 12
- 392
- 0
Báo cáo khoa học: Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression potx
... treatments are: untreated control (A), 50 lM MIBA (B) and MIBG (C), 100 lM MIBA (D) and MIBG (E), 200 lM MIBA (F) and MIBG (G), and 500 lM MIBA (H) and MIBG (I) Magnification, 120 · Fig Sensitivity ... the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also showed ... [32P]orthophosphate for h prior to treatment with MIBG (50 lM) for 10 followed by addition of PGE2 (1 lM) or TxA2 (0.1 lM) for 15 Phosphoinositides were extracted from the cells and the phosphorylated forms...
- 10
- 389
- 0
Báo cáo khoa học: Extraenzymatic functions of the dipeptidyl peptidase IV-related proteins DP8 and DP9 in cell adhesion, migration and apoptosis doc
... DP8 and DP9 D M T Yu et al Table Primers used for generating green fluorescent protein (GFP) fusion constructs and for mutagenesis Primer GFP fusion DP8SalIfus .For DP8KpnIfus.Rev DP9EcoRI .For ... Kable and Devanshi Seth for advice This work was supported by National Health and Medical Research Council of Australia project grant 142607 to GWM and MDG and PhD scholarships to DMTY and XMW ... enzymatic activities of DP8 and DP9 are not required for their effects on adhesion, wound healing and apoptosis Similarly, the enzyme activities of DPIV and FAP are not required for their 2454 102 CFP...
- 14
- 280
- 0
Báo cáo khoa học: Cleavage of focal adhesion proteins and PKCd during lovastatin-induced apoptosis in spontaneously immortalized rat brain neuroblasts ppt
... without Ca2+ and Mg2+ and then scraped and pelleted at °C Cells were lysed in Tris buffer (10 mm Tris, pH 7.4, mm EDTA, and 0.5% Triton X-100) for 60 at °C After centrifugation at 10 000 g for 30 at ... °C for 45 min, and then with proteinase K (0.2 mgÆmL)1) at 37 °C for 45 DNA was then extracted twice with phenol ⁄ chloroform (1 : 1) and precipitated with 0.1 volumes of sodium acetate (3 m) and ... least 11 isoforms of PKC have been identified and can be classified into conventional (a, bI, bII and c), novel (d, e, g, and h), atypical (f and k ⁄ i) and novel ⁄ atypical (l) based upon their cofactor...
- 13
- 364
- 0
Báo cáo khoa học: Lipid droplet and milk lipid globule membrane associated placental protein 17b (PP17b) is involved in apoptotic and differentiation processes of human epithelial cervical carcinoma cells pptx
... cells, B, D, and F paclitaxel (10 nM for 24 h) treated cells, and H dbcAMP (0.5 mM for 72 h) treated cells In A, B, G and H, cells were stained with PP17, in C and D with anti-bax, in E and F with ... purified 30-, 48- and 60-kDa proteins Protein identification and sequencing are described in Materials and methods Most of the input masses matched candidate protein sequences, and the majority ... for the ÔPP17/TIP47Õ gene; and (e) there is a common need to elucidate this controversial situation, it is therefore now proposed that the PP17 variants be renamed to sandrin A (PP17a) and sandrin...
- 13
- 371
- 0
Adhesion science and engineering volume 2, the mechanics of adhesion surfaces, chemistry applicat
... surface treatments for the purpose of eliminating unpromising candidates for what is generally tedious and costly mechanical testing The results of such attempts may vary from the formulation of ... underpinning for a relationship between practical adhesion energy and the work of adhesion is the generalized fracture mechanics theory of Gent and coworkers [23-251 and contributed to by Andrews and ... be the same as that between 6, and U for forced spreading [59] Both theoretical and empirical studies of this rate process have been reported [60-62], and most conform to what is now known as Tanner's...
- 1,226
- 1,200
- 0
Báo cáo sinh học: "Protein kinase CK2a is overexpressed in colorectal cancer and modulates cell proliferation and invasion via regulating EMT-related genes" docx
... designed and performed experiments JZ and HL performed the statistical analysis and drafted the manuscript DW and LL helped in drafting the manuscript and contributed specific information and critical ... p53, p21 and C-myc expression CK2a knockdown inhibits cell migration and invasion Migration and matrigel invasion assays were performed to examine the effect of CK2a on tumor cell migration and invasion, ... ng/ml) for h, and stained for immunofluorescence with b-catenin antibody (red) and DAPI (blue) (B) Western blot was used to detect the expression levels of CK2a, E-cadherin, b-catenin, vimentin and...
- 11
- 382
- 0
báo cáo hóa học:" Effect of borax on immune cell proliferation and sister chromatid exchange in human chromosomes Malinee Pongsavee" docx
... Medicine and Toxicology 2009, 4:27 http://www.occup-med.com/content/4/1/27 (PBS) were added to each well and incubated for hours at 37°C The medium was removed and formazan was dissolved in DMSO and ... active, and therefore conversion can be directly related to the number of living cells When the amount of purple formazan produced by cells treated with an agent is compared with the amount of formazan ... Bio-assay reader MTT assay of forty lymphocyte cultures in each borax concentration was carried out in duplicate fashion Mean absorbance was calculate for the control wells and for each borax concentration...
- 6
- 580
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25