typhimurium a1 r in pc 3 human prostate cancer cells in vitro
Báo cáo khoa học: " Radiosensitization by 2-benzoyl-3-phenyl-6,7-dichloroquinoxaline 1,4-dioxide under oxia and hypoxia in human colon cancer cells" pdf
Ngày tải lên :
09/08/2014, 10:21
... cell survival in DCQ+IR in normal FHs74Int v.s DLD-1 cancer cells confirms the greater radio-sensitizing effects of this drug in cancer cells DNA damage by DCQ in irradiated DLD-1 cells under oxia ... cycle arrest at G2/M phase In oxic cells, p-Akt protein expression levels increased upon exposure to IR; pretreatment with DCQ inhibited this increase in p-Akt protein (Figure 8C) In contrast, in ... fold increase) Exposure of HCT-116 cells to oxic or hypoxic conditions prior to IR resulted in no difference in their sensitivity to the drug (% of cells in preG1 phase was 36 % in oxia and 23% in...
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EXPRESSION ANALYSIS AND FUNCTIONAL STUDY OF HS3ST3B1 IN HUMAN PROSTATE CANCER
Ngày tải lên :
05/10/2015, 22:31
... the protein level Figure 3. 4 Immunofluorescence staining of HS3ST3B1 56 Figure 3. 5 HS3ST3B1 increased RWPE-1 proliferation 58 Figure 3. 6 HS3ST3B1 increased RWPE-1 migration 60 Figure 3. 7 HS3ST3B1 ... Clinicopathological parameters of prostate 77 cancer patients in study 3. 4.2 Expression of HS3ST3B1 in prostate cancer 79 3. 4 .3 Associations of HS3ST3B1 immune reactive 80 scores in prostate cancer ... HS3ST3B1 in 51 prostate cancer 3. 1.1 Expression of HS3ST3B1 in prostate cell lines 51 and tissues 3. 2 Functional analysis of HS3ST3B1 in prostate cancer 53 3.2.1 HS3ST3B1 is effectively silenced in...
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Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt
Ngày tải lên :
06/03/2014, 22:21
... membrane-binding protein FK506-binding protein 1A Metallothionein-1k suc1-associated neurotrophic factor target (fibroblast growth factor receptor substrate 2) Small GTP-binding protein rab22b Minichromosome ... maintenance protein homolog Ribosomal L1 domain containing Hepatocellular carcinoma-associated protein HCA10 Chromosome 20 ORF 30 Splicing factor proline ⁄ glutamine rich (polypyrimidine tract-binding ... Tca81 13 by normalizing against b-actin Bars and standard errors representing relative expression of tested genes normalized against b-actin in the data were obtained from three independent experiments...
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Báo cáo khoa học: MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells potx
Ngày tải lên :
07/03/2014, 00:20
... miR-1 43 vectors, anti-miR-1 43 inhibitor, pre-miR-1 43 and ERK5 siRNA HCT116 cells were transiently transfected with miR-1 43 overexpression vector coding for the miR-1 43 precursor (pCR3-pri-miR-1 43) ... for 72 h Mixed populations of miR-1 43 overexpressing cells (pCR3-pri-miR-1 43 mix) and respective control cells (pCR3-empty mix) were generated by transfecting HCT116 cells with pCR3-pri-miR-1 43 ... normalized to MTS for pre-miR-1 43 and anti-miR-1 43 compared to the respective controls Black bar, ratio of pre-miR-1 43 over pre-miR-control; white bar, ratio of anti-miR-1 43 over anti-miR-control...
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Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
Ngày tải lên :
07/03/2014, 05:20
... pcDNA3.1 plasmid (Invitrogen, Carlsbad, CA, USA) or with the control vector (pcDNA3.1 Zeo) using Lipofectamine PLUS reagent (Invitrogen) according to the manufacturer’s recommendations Cells were ... that there is a time-dependent increase in apoptotic cell death, reaching approximately 35 % of cells after 72 h of treatment To determine whether death receptors are involved in radiation-induced ... a pro-apoptotic kinase in response to a variety of different stimuli, including tumor necrosis factor, UV irradiation, cytokine, Role of JNK in radiation-induced mitochondrial cell death ceramide,...
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Báo cáo khoa học: Modulation of IMPDH2, survivin, topoisomerase I and vimentin increases sensitivity to methotrexate in HT29 human colon cancer cells docx
Ngày tải lên :
07/03/2014, 16:20
... Belldegrun A, Persad S & Singh G (20 03) Overexpression of vimentin: role in the invasive phenotype in an androgen-independent model of prostate cancer Cancer Res 63, 230 6– 231 1 49 Wilson KS, Roberts ... also increased in the arrays from cells treated with MTX or HT29 -R Overexpression of GARFT in treated and resistant cells was also validated by RT-PCR (data not shown) Thus, the increases in GARFT ... Apoptosis associated proteins Retinoid X receptor beta; retinoic acid X receptor b WSL-S2 protein; WSL-S1 protein; WSL- 1R protein RXRB TNFRSF12 M84820 Y0 939 2 P28702 Q 930 38 0.11 0.14 0 .30 0.11 Cell cycle...
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Báo cáo Y học: Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine pdf
Ngày tải lên :
17/03/2014, 23:20
... of DENSPM treatment There are reports of p 53- deficient cells that are inhibited in their growth with no apoptotic response by treatment with DENSPM [32 ,33 ] Other cellular defects are involved and ... (Weber, G., ed.), pp 57–79 Pergamon Press, New York Porter, C.W., Ganis, B., Libby, P .R & Bergeron, R. J (1991) Correlations between polyamine analogue-induced increases in spermidine/spermine ... (19 93) Effects of diethyl spermine analogues in human bladder cancer cell lines in culture J Urol 150, 12 93 1297 10 Bernacki, R. J., Oberman, E.J., Seweryniak, K.E., Atwood, A., Bergeron, R. J & Porter,...
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Báo cáo sinh học: " Downregulation of APOBEC3G by Xenotropic Murine Leukemia-Virus Related Virus (XMRV) in Prostate Cancer Cells docx
Ngày tải lên :
18/06/2014, 18:20
... supported in part by grants to CD (R0 0DA024558, R0 3DA30896) from NIH, (UL1 RR024975) from the Vanderbilt CTSA Grant and (U54RR026140) Meharry Translational Research Center (MeTRC) References Urisman ... a rare recombination event Host restriction factor APOBEC3G (A3G) has been reported to severely restrict XMRV replication in human peripheral blood mononuclear cells Interestingly, XMRV infects ... degrade A3G Figure XMRV induced downregulation of A3G in prostate cancer cells We used culture supernatant from chronically infected LNCaP cells with XMRV as the source of infectious XMRV Virus infections...
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Báo cáo khoa học: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells" doc
Ngày tải lên :
09/08/2014, 03:21
... improved overall survival rate in oral squamous cell carcinoma [37 ] Our finding of conformational change of Bcl-2 in SGC7901 cells following exposure to arsenic trioxide is important for founding ... Cancer Chemother Pharmacol 2008, 63( 1) :37 - 43 Florea AM, Busselberg D: Anti -cancer drugs interfere with intracellular calcium signaling Neurotoxicology 2009, 30 (5):8 03- 810 Deng Y, Lin C, Zheng J, ... cells per square centimeter and cultured for 24 hours Cells were starved for 24 hours by replacing the media with serum-free media containing 0.1% BSA, followed by exposure to different concentrations...
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Báo cáo khoa học: "Cell death by the quinoxaline dioxide DCQ in human colon cancer cells is enhanced under hypoxia and is independent of p53 and p21" pdf
Ngày tải lên :
09/08/2014, 09:20
... culture and treatments FHs74Int normal human intestinal cells were cultured in Hybri-Care medium supplemented with 30 ng/ml epidermal growth factor HCT116 (p 53+ /+) human colon cancer cells were ... correlated with an increase in the pro-apoptotic caspase-2 protein, inhibition of the pro-survival protein PIDD-C, and increase in p-ATM expression, a major protein kinase involved in repair of DSB DCQ ... of p 53, p21, PIDD-C and caspase-2 proteins Cells were exposed to or 10 μM DCQ and protein changes were monitored hrs post-treatment under normoxia or hypoxia The p 53 protein increased in response...
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Báo cáo khoa học: "The Akt-inhibitor Erufosine induces apoptotic cell death in prostate cancer cells and increases the short term effects of ionizing radiation" pot
Ngày tải lên :
09/08/2014, 09:20
... paradigmatic intravenously applicable alkylphosphocholine ErPC3 potently induces apoptosis in prostate cancer cells in vitro These findings corroborate earlier reports on high efficacy of ErPC3 ... protein family members in response to ErPC3-treatment and irradiation PC3 and LNCaP cells were treated with 0-25 µM ErPC3 or irradiated with or 10 Gy Cells lysates were generated 48 h after treatment, ... The AKT inhibitor perifosine in biochemically recurrent prostate cancer: a phase II California/ Pittsburgh cancer consortium trial Clin Genitourin Cancer 2007, 5: 433 - 437 Alessi DR, James SR, Downes...
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Báo cáo khoa học: "The alkylphospholipid, perifosine, radiosensitizes prostate cancer cells both in vitro and in vivo" docx
Ngày tải lên :
09/08/2014, 09:20
... Pefirosine 30 M Figure Perifosine increases prostate cancer radiosensitivity in vitro A, CWR22RV1 cells were irradiated in the absence (control) or the presence of 10 μM perifosine for 24 hours and the ... alkylphospholipid perifosine in CWR22RV1 prostate cancer both in vitro and in vivo In vitro, perifosine reduced cell viability and clonogenic survival, and enhanced apoptosis after radiation In vivo, substantial ... http://www.ro-journal.com/content/6/1 /39 Page of Perifosine enhances prostate cancer radiosensitivity in vivo A Control IR perifosine Combined Additive effect D B Control Perifosine 15 M Pefirosine 30 ...
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Báo cáo khoa học: "In vitro studies on the modification of low-dose hyper-radiosensitivity in prostate cancer cells by incubation with genistein and estradiol" pot
Ngày tải lên :
09/08/2014, 09:22
... expression controls genistein or estradiol RT genistein or estradiol RT RT + genistein or estradiol G0/G1 arrest G0/G1 arrest G0/G1 arrest no influence G2/M arrest G2/M arrest reduced PC- 3 FACS ... endocrine therapy in breast cancer Endocr Relat Cancer 20 03, 10 :37 5 -38 8 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Villalobos M, Becerra D, Nunez MI, Valenzuela MT, Shiles E, Olea N, Pedraza ... after incubation with genistein (LNCaP 48 h incubation, PC- 3 24 h) Survival was expressed relative to untreated controls Error bars represent standard errors In both cell lines a significant reduction...
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báo cáo khoa học: " XAF1 expression and regulatory effects of somatostatin on XAF1 in prostate cancer cells" docx
Ngày tải lên :
10/08/2014, 10:20
... Controls were untreated cells RNA extraction and RT-PCR XAF1 mRNA was detected using reverse transcription PCR (RT-PCR) Total cellular RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, ... a promising strategy for prostate cancer therapy Abbreviations List NE: neuroendocrine; RWPE-1: human prostate epithelial cell line; SSTR: somatostatin receptor; XAF1: X-linked inhibitor of apoptosis ... regulatory effects of XAF1 in many different types of human cancers Three prostate cancer cell lines LNCaP, DU145 and PC3 were well established in laboratory experiments Their invasive characteristics...
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báo cáo khoa học: " Salivary a-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells" docx
Ngày tải lên :
10/08/2014, 10:21
... histocompatible inbred rat strains originating from the same background strain [28], but with differing responses towards stress [30 ,41], indicating a stronger stress response of F344 compared to Lewis rats ... to alter growth of mammary epithelial cells by using primary cultures of rat origin For this purpose, we used primary mammary epithelial cells from two inbred rat strains, Fischer 34 4 (F344) and ... McEwen BS, Spencer RL: Stress response, adrenal steroid receptor levels and corticosteroid-binding globulin levels - a comparison 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49...
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báo cáo khoa học: "Possible immunotherapeutic potentiation with D-Fraction in prostate cancer cells" pps
Ngày tải lên :
10/08/2014, 22:20
... serine/threonine protein kinase DNA-PK Crit Rev Eukaryot Gene Expr 1992, 2:2 83- 314 Kramer G, Steiner GE, Sokol P, Handisurya A, Klingler HC, Maier U, Foldy M, Marberger M: Local intratumoral tumor necrosis ... Haelst-Pisani CM, Richardson RL, Su J, Buckner JC, Hahn RG, Frytak S, Kvols LK, Burch PA: A phase II study of recombinant human alpha-interferon in advanced hormone-refractory prostate cancer Cancer 1992, ... effects of brefeldin A (BFA) on cell cycle progression involving the modulation of the retinoblastoma protein (pRB) in PC- 3 prostate cancer cells J Urol 1998, 159:275-279 References 10 11 12 13 14 15...
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báo cáo khoa học: "Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP" pptx
Ngày tải lên :
11/08/2014, 00:22
... Tumor Angiogenesis in Gastric Cancer Cancer Microenviron 2009 in press 14 Waugh DJ, Wilson C: The interleukin-8 pathway in cancer Clin Cancer Res 2008, 14(21):6 735 -41 15 Nishimoto N: Interleukin-6 ... and interpretation and in writing and revising the manuscript RPR assisted in the design of all experiments and in writing and revising the manuscript All authors have read and approved the final ... Interleukin-6 in rheumatoid arthritis Curr Opin Rheumatol 2006, 18 (3) :277-81 16 Dayer JM, Choy E: Therapeutic targets in rheumatoid arthritis: the interleukin-6 receptor Rheumatology (Oxford) 2010,...
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Báo cáo y học: "Analysis of XMRV integration sites from human prostate cancer tissues suggests PCR contamination rather than genuine human infection" ppt
Ngày tải lên :
13/08/2014, 01:20
... possibly the result of somatic mutation in the cell line during its replication or the result of PCR error during amplification PCR error is not unlikely given the three step nested PCR protocol and ... to contamination from human tumour cell lines infected with XMRV (e.g 22Rv1) or other xenotropic MLVs acquired by xenografting in mice [6] Integration of XMRV into human chromosomes Central to ... liquid chromatography; LTR: long terminal repeat; MLV: murine leukaemia virus; PCR: polymerase chain reaction; XMRV: Xenotropic murine leukaemia virusrelated virus Acknowledgements and funding This...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
Ngày tải lên :
14/08/2014, 08:21
... tumor signatures are found in human prostate cancers containing a minimum Gleason score pathology Distinctive stem cell and tumor signatures are found in human prostate cancers containing a minimum ... seen in prostate tumors The cancer stem cell content was not, however, dependent on prostate tumor clinical stage or grade Numerous groups have profiled prostate cancer using DNA microarrays (reviewed ... (hormone refractory (HR)) by androgen blockade therapy turer's instructions The RNA was reverse transcribed using random hexamers and reverse transcriptase (Superscript III, Invitrogen, Paisley,...
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