twilight of the gods and tally ho

... of doze, but it was only for a moment, and then he dreamt he had dug a deep hole into the earth and poured in tons and tons of the Food of the Gods, and the earth was swelling and swelling, and ... in hand— to commence the scooting of the giant hens They drove them into Urshot, where there was a Rural Fête, and Urshot took them as the crowning glory of a happy day They began to be shot ... But Skinner, standing at the bar and drinking his hot gin and water, with one eye roving over the things at the back of the bar and the other fixed on the Absolute, missed the psychological moment...

Ngày tải lên: 22/03/2014, 23:20

... Following the suggestions of scientific literature on the advantages of the compactness, reliability, ease of use and affordability of the 980 nm Diode Lasers, we evaluated the efficacy and safety of ... question the efficacy of the 980nm Laser for photosclerosis of lesions and areas with little melanin and hemoglobin pigmentation [7] Furthermore, to avoid exaggerated fluences and thermal damage to the ... are reduced [17,18] The lasers normally adopted offer the enormous advantage of being conveyed in a fibre, which serves as the working tip at the end of the handpiece The use of flexible, length-adjustable...

Ngày tải lên: 26/10/2012, 09:48

... PS-2 (Fig 6) The proton spectrum of PS-2 was more homogeneous and had sharper lines than the proton spectrum of PS-1 As the spectrum of PS-2 was less complex than the spectrum of the native PS-1, ... spectrum of the ring protons In (g) is the HMBC spectrum showing the C¼O region for assignment of C-6B and C-7C The HMBC spectrum in (h) shows the interglycosidic 3J(C,H) correlations In (i) the proton ... version of the gene (results not shown) Examination of LOS cores isolated from growth of single colonies of our NCTC 11168 by deoxycholate-PAGE and MS yielded structures with and without the terminal...

Ngày tải lên: 21/02/2014, 01:21

... that the activity of SFKs is tightly regulated by the reversible phosphorylation of Y527 in chicken c-Src in vivo The phosphorylation of Y527 may decrease the activity of SFKs, with dephosphorylation ... reduces auto-phosphorylation by modulating the kinase activity of the enzyme To determine the roles of the SH3 and ⁄ or SH2 domains in Src regulation of NMDAR phosphoryla- tion, the protein fragment ... Dysfunction of the SH3 and ⁄ or SH2 domains affected the phosphorylation process of NR2A C-tail proteins by active n-Src and reduced the n-Src activity on NMDARs, with the greater effect produced by the...

Ngày tải lên: 06/03/2014, 01:20

... The Future of the Internet— And How to Stop It The Future of the Internet And How to Stop It Jonathan Zittrain With a New Foreword by Lawrence Lessig and a New Preface by the Author Yale ... attractive aesthetic, and it bottles some of the best innovations from the PC and Internet in a stable, controlled form The PC and Internet were the engines of those innovations, and if they can be ... become, Zittrain puts the crud right in the center And he then builds an understanding of the Net, and the computers that made the Net possible, that explains how so much good and so much awful could...

Ngày tải lên: 07/03/2014, 03:20

... bind to all of the tRNA binding sites on the ribosome (i.e A, P and E sites) [13,14] As expected, the addition of SF to the system resulted in pppGpp synthesis (Fig 2) The speed of synthesis, calculated ... From these results it can be concluded that tRNAs were bound in classical states with the tRNAMet bound f in the P-sites of the 30S and 50S subunits and the tRNAPhe bound in the A-site of the ... 2005 FEBS R.-M Knutsson Jenvert and L Holmberg Schiavone Fig Autoradiograms showing the template and tRNA-dependence of the pppGpp-synthesis reaction The activity of TC-ribosomes (0.67 lM, lane...

Ngày tải lên: 07/03/2014, 16:20

... bound to the s-site only, and inactive mR14b, with d-ATP bound to the s- and a-sites [2] Addition of l-ATP should lead to saturation of the a-site and complete conversion to mR14b, leading to the ... demonstrating the considerable stringency at the active site of mR1 with respect to catalytic activity On the other hand, our results show that l-ADP binds to the active site and l-ATP binds to both the ... Dutschman G & Cheng YC (2002) Phosphorylation of pyrimidine deoxynucleoside analog diphosphates: selective phosphorylation of 1-nucleoside analog diphosphates by 3-phosphoglycerate kinase J Biol Chem...

Ngày tải lên: 07/03/2014, 17:20

... to the next, the levels of +2 and +3 ion species of phosphorylated fragment were expressed as a percentage of the combined ion count from both the phosphorylated and unphosphorylated forms of ... show the different mass species present in sample phosphorylated for (left panel) and 30 (right panel) of phosphorylation The modal mass of the nonphosphorylated His-cTCRf is 14 274 Da, and the ... and their masses determined to assess the level of phosphorylation For the unphosphorylated sample shown in Fig 3, the predominant mass was 14 274 Da (24 Da less than the mono-isotopic mass of...

Ngày tải lên: 08/03/2014, 02:20

Ngày tải lên: 08/03/2014, 10:20

... Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt of the N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending on the position of the ... from the orientation of (A) The colours of the two monomers (yellow and grey) and of the hydrophobic residues (green) are as in (A) The dense hydrophobic face (green atoms) is the binding site of ... interface of the two monomers (Fig 6B) A further rotation of the orientation of RIIa D/D of Fig 4B by 180° about the x-axis shows the bottom face of RIIa D/D (Fig 4C) There is some hydrophobic character...

Ngày tải lên: 08/03/2014, 22:20

... change in the conformation of this loop Changes in the conformation of the backbone at this point and changes in the orientation of other side chains, as a result of the introduction of the large ... introduced alanine at these two positions, with the aim of determining whether any of the observed effects were a result of changes in the size and charge of the amino acid at these positions Results ... In view of the structural similarities between yeast and mammalian enolases and the high degree of sequence conservation, we believe that the effects of these mutations on the structure and function...

Ngày tải lên: 16/03/2014, 06:20

... around the ligand-binding site of liganded hemoglobin The conformation of Val(E11) in the a and b subunits relative to the heme plane is quite dependent on the nature of the anions and the pD of the ... The time course and the yield of the geminate phase are both sensitive to the immediate environment of the heme, and to the dynamics of structural changes in the protein Hence, the analysis of ... yield of BR (the efficiency of the ligand escape) for the a and b subunits within the oxygenated tetramer and dimer of human hemoglobin were revealed As a consequence, the regulation of the affinity...

Ngày tải lên: 16/03/2014, 14:20

... near the N-terminus of helix I of the domain and the three residues in loop L2 contributing to the E3-binding site Backbone dynamics of PSBD The region (Met132 to Ser134) before the start of helix ... Number Fig NMR spectroscopy of the interaction of PSBD and E3int Plots of the (A) 15N and (B) 1HN chemical shift changes between the [15N,1H]-HSQC spectra of free PSBD and the PSBD–E3int dimer (10 ... complex of E coli [12] and of the dihydroacetyltransferase chain of the PDH complex of B stearothermophilus [13] The domain consists of two a-helices comprising residues Ser134 to Lys142 (helix I) and...

Ngày tải lên: 16/03/2014, 18:20

... and acetate (200 mM) at 25 °C Note that the activity in the cell free extract is the sum of CoA-transferase and phosphotransacetylase and that the latter enzyme is completely separated from the ... subunit of glutaconate CoA-transferase are given in black, those homologous to the b subunit in white The a helices are shown by circles and the b sheets by triangles The position of the catalytic ... search, this part of the sequence showed similarity to the smaller subunits of the latter enzymes The catalytic glutamate residue of hetero-oligomeric enzymes belonging to the 3-oxoadipate CoA-transferase...

Ngày tải lên: 17/03/2014, 17:20

... visible (B) regions The solid line shows the spectrum of ferric H42A; the dotted and dashed lines show the intermediate spectra derived from the model, and represent the spectra of the proposed [H42A–H2O2] ... for the Compound I intermediates of H42A and H42E were similar to those of rAPX and are also consistent with a porphyrin p-cation formulation The decay of Compound I of rAPX in the absence of ... in the absence of exogenous imidazoles Formation of Compound I again showed saturation kinetics (Fig 6), and a nonlinear least-squares fit of the data to Eqn (7) in the presence of imidazole and...

Ngày tải lên: 18/03/2014, 01:20

... at the two spatial tips of the molecule, at the N-terminus with Arg1 and in the turn in the presence of Arg7 and Arg9 (see Figs and 4) The shorter analogue, BM-2, was the most flexible of all the ... formed the second face of the peptide Two hydrophilic regions were located at the two spatial tips of the molecule, at both termini with Arg1 and Arg17, and in the turn in the presence of Arg8 and ... hydrophobic [46] There is a large difference in the distribution of hydrophobic ⁄ hydrophilic potentials on the b-sheet surface between the tails and the turn The central portion of IB-367, BM-1 and...

Ngày tải lên: 22/03/2014, 21:20

... Structure of human GCPIII K Hlouchova et al of the S1-bound chloride anion and ‘pushes’ the Cl) out of the bottom of the S1 pocket (the occupancy of the Cl) in this structure is 0.7) The alternate ... with the occupancy of the S1 pocket by a ligand and the ‘open–close’ transition exploits the hinges on the two sides of the lid made up of Asn540 ⁄ Trp541 and Gly548 (GCPII numbering) In the GCPIII ... Asn628-NAG2 of one monomer and the side chain of Glu266 of the apical domain of the second monomer The binuclear metal center Electron density maps reveal the presence of two metal ions in the active...

Ngày tải lên: 23/03/2014, 05:22

... Encouraged by the results obtained, and to investigate the mechanism of macrocyclization, the steric demand of the C-terminal amino acid was further increased by the incorporation of l-Met4 (TL4) showing ... [11] The resulting structure can be either branched-cyclic, as in the case of the last-line antibiotic daptomycin, or closed, as in the case of the antibiotic tyrocidine A [12,13] The nature of the ... cyclization ratio of : Additionally, the formation of the linear octapeptidyl-SNAC Lig10 was detected, reflecting the steric demand of l-Met4 To investigate the influence of the chromophore moiety on...

Ngày tải lên: 23/03/2014, 06:20

... models of the elements representing the ligand-binding domain and EGF precursor homology domain of locust LpR bear a striking resemblance to those of mammalian LDLR [10] On the other hand, the ligand-binding ... resecretion The stability of the complex and the modulation thereof may be determined by secondary contacts between HDLp and nonconserved residues of LpR Although the function of recycling of endocytosed ... proportional to the amount of LDLR on the plasma membrane However, in the case of the hybrid receptor LDLR(1–251)LpR(302–850) (Fig 4F), the ligand-binding domain of which is composed of the six most...

Ngày tải lên: 23/03/2014, 07:20

... of HU proteins from B stearothermophilus [20] and T maritima [18] The upper part of the figure shows the aligned amino acid sequences of each monomer within the homodimers, the ribbon models of ... dependence of the heat capacity of the initial and final conformations (Cp,N2 and Cp,U), the heat capacity of the native state was taken to be a linear function of temperature and that of the unfolded ... some of the experimental and mathematical bases for future studies into strategies for increasing the thermal stability of proteins Materials and methods Overproduction and purification of HU...

Ngày tải lên: 23/03/2014, 12:20