transport across cell membranes table 1 1

... the cell, thus the net flow of oxygen is into the cell Movement of selected molecules across the cell membrane The above image is from http://www.biosci.uga.edu/almanac/bio _10 3/notes/may _13 .html ... or transport chemicals out of the cell or to allow them to enter a cell Exocytosis is the term applied when transport is out of the cell ► Endocytosis is the case when a molecule causes the cell ... major step in the evolution of the cell Cell membranes are differentially (or semi-) permeable barriers separating the inner cellular environment from the outer cellular (or external) environment...

Ngày tải lên: 24/07/2014, 19:20

... translocation Cell 73, 11 01 11 15 10 Matlack KE, Misselwitz B, Plath K & Rapoport TA (19 99) BiP acts as a molecular ratchet during posttranslational transport of prepro-a factor across the ER membrane Cell ... membrane Cell 75, 615 –630 Simon SM & Blobel G (19 91) A protein-conducting channel in the endoplasmic reticulum Cell 65, 3 71 380 Crowley KS, Liao SR, Worrell VE, Reinhart GD & Johnson AE (19 94) ... Nature 418 , 662–665 13 Plath K, Mothes W, Wilkinson BM, Stirling CJ & Rapoport TA (19 98) Signal sequence recognition in posttranslational protein transport across the yeast ER membrane Cell 94,...

Ngày tải lên: 07/03/2014, 06:20

... J Cell Biol 10 1, 11 98 12 11 16 Kiang JG, Carr FE, Burns MR & McLain DE (19 94) HSP-72 synthesis is promoted by increase in [Ca2+]i or activation of G proteins but not pHi or cAMP Am J Physiol Cell ... Dead cells were excluded by forward and side scatter gating JC -1 aggregates were detectable in the FL2 (585 ± 21 nm), and JC -1 monomers were detectable in the FL1 (530 ± 15 nm) channel Data on 10 4 ... survival of heat shock J Appl Physiol 92, 17 43 17 48 10 Park HG, Han SI, Oh SY & Kang HS (2005) Cellular responses to mild heat stress Cell Mol Life Sci 62, 10 –23 11 Carratu L, Franceschelli S, Pardini...

Ngày tải lên: 07/03/2014, 12:20

... 10 0 10 1 10 2 10 3 10 4 10 0 10 0 Ex vivo 10 1 10 2 10 3 10 4 10 0 10 0 10 1 OML/Tax 10 2 10 3 10 4 Peptide 10 0 10 0 10 1 10 2 10 3 10 4 None CD8 B 60 % specific lysis 50 40 HTLV -1 CMV T2-A2 only 30 20 10 1: 1 5 :1 ... T2-A2 cells, whereas only low-level background lysis was observed in the absence of Tax A 10 4 10 4 10 4 0. 41% 10 4 3.46% 0.02% 0 .1% 10 3 10 3 10 3 10 2 HTLV -1 tetramer 10 3 10 2 10 2 10 2 10 1 10 1 10 1 10 1 10 0 ... 0. 01 vs unpulsed iDC controls OML/Tax Peptide PBS 30 OML ⁄ Tax (%) 20 MHC Class I HLA-A02 MHC Class II CD86 10 2.5 :1 5 :1 PHA (%) 208.5 12 1.0 11 5.2 13 1.8 12 9.8 10 2.7 10 3.3 10 9.0 652.6 17 6.2 13 0.1...

Ngày tải lên: 14/03/2014, 23:20

... SDF -1/ CXCL12 A B N Charnaux et al C 32 32 IgGl (SD-4ds RNA) SD -1 (SD-4 ds RNA) IgGl (mocktransfected) 0 10 10 1 10 2 10 3 10 0 SD -1 (mocktransfected) 10 1 10 2 10 3 10 4 E D 32 0 10 10 1 10 2 10 3 10 4 Fig ... 2 411 3–2 412 3 15 Carey DJ (19 97) Syndecans: multifunctional cell- surface co-receptors Biochem J 327, 1 16 16 Couchman JR, Chen L & Woods A (20 01) Syndecans and cell adhesion Int Rev Cytol 207, 11 3 15 0 ... 272, 11 805 11 811 David G, Bai XM, Van der Schueren B, Cassiman JJ & Van den Berghe H (19 92) Developmental changes in heparan sulfate expression: in situ detection with mAbs J Cell Biol 11 9, 9 61 975...

Ngày tải lên: 16/03/2014, 18:20

... phospholipase A2 J Cell Biol 14 5, 12 19 12 32 17 Rizo, J & Sudhof, T.C (19 98) C2-domains, structure and function of a universal Ca2+-binding domain J Biol Chem 273, 15 879 15 882 18 Davletov, B., Perisic, ... X -11 4 was performed A solution of Triton X -11 4 is homogenous at temperatures Fig EGTA-resistant binding of cPLA2-a to EA.hy.926 cell membranes (A) Cells were stimulated with lM A2 318 7 for 10 ... Biol Chem 275, 11 61 11 68 41 Croxtal, J.D., Newman, S.P., Choudhury, Q & Flower, R.J (19 96) The concerted regulation of cPLA2, COX2, and lipocortin expression by IL-1b in A549 cells Biochem Biophys...

Ngày tải lên: 30/03/2014, 13:20

... (semen:extender) Sperm density (cells/mL) 1: 2 1: 3 8.0 × 10 8–2.5 × 10 9 4.0 × 10 9–8 × 10 9 1: 5 1: 7 9.0 × 10 9 1. 5 × 10 10 1. 0 × 10 10–2 × 10 10 be adjusted to different distances (0 10 cm) above the surface ... (3–4) × 10 6 Fertility at sperm-to-egg ratio (1. 5–2) × 10 6 Fresh Frozen/Thawed 4.8 ± 3.3a 9.5 ± 4.4a 85.7 ± 12 .2a 52.8 ± 10 .6a 29 .1 ± 8.0a 18 .1 ± 11 .1a 90.3 ± 17 .2la 79.6 ± 12 .2a 79.8 ± 10 .5a 71. 3 ... Chemotaxis 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 19 Rotary evaporator Freeze-dryer Centrifugal vaporizer Centrifuge Artificial seawater (ASW): 462 mM NaCl, mM KCl, 11 mM CaCl2,...

Ngày tải lên: 11/04/2014, 09:43

... xv Chapter 1. 1 1. 2 1. 3 1. 4 Examples of Contaminated Sediment Sites 1. 1 .1 Hudson River 1. 1.2 Lower Fox River 1. 1.3 Passaic River/Newark Bay 1. 1.4 Palos Verdes ... Flow Rate (m3/s) 15 00 10 00 500 19 40 19 50 19 60 19 70 19 80 19 90 Year FIGURE 1. 5 Saginaw River: flow rate as a function of time from 19 40 to 19 90 (Source: From Cardenas et al., 19 95 With permission.) ... Conditions 17 6 5 .1. 2 Eddy Coefficients 17 9 5 .1. 3 Bottom Shear Stress 18 2 5 .1. 3 .1 Effects of Currents 18 2 5 .1. 3.2 Effects of Waves and Currents 18 5 5 .1. 4 Wind Stress...

Ngày tải lên: 18/06/2014, 22:20

... 30 10 20 10 750 10 00 12 50 15 00 17 50 Engine speed, rpm 2000 2250 Fig Engine load-speed map for ETC 10 0 90 40 Engine load, % 80 30 70 60 20 50 40 10 30 frequency, WHTC 20 10 750 10 00 12 50 15 00 17 50 ... load-speed map with SCR system 10 0 16 80 Engine load, % 90 14 70 12 60 10 50 NOx matrix (gr), test10 40 30 20 10 750 10 00 12 50 15 00 17 50 Engine speed, rpm 2000 2250 Fig 10 Cycle NOx emissions to be ... flow meter) 8 Urban Transport and Hybrid Vehicles 10 0 90 Engine load, % 80 0.4 70 0.35 NOxmatrix gr/s, test15 60 0.3 0.25 50 0.2 40 0 .15 30 0 .1 20 0.05 10 750 10 00 12 50 15 00 17 50 Engine speed,...

Ngày tải lên: 20/06/2014, 12:20

... number and types of cultured stem cells have increased progressively (Table 66 -1) The cultured stem cells derived from resident stem cells are often called adult stem cells to indicate their adult ... neural stem (NS) cells, it is more appropriate to use the term, tissue stem cells Table 66 -1 Types of Cultured Stem Cells Name Source, Derivation, Maintenance, and Properties Embryonic cells (ES, ESC) ... Stem Cells Unequivocal identification of stem cells requires the separation and purification of cells, usually based on a combination of specific cell- surface markers These isolated stem cells,...

Ngày tải lên: 07/07/2014, 01:20

... small cell tumors (with paraneoplastic syndromes)

Ngày tải lên: 07/07/2014, 02:20

... is lost The clinical manifestations of all the plasma cell disorders relate to the expansion of the neoplastic cells, to the secretion of cell products (immunoglobulin molecules or subunits, lymphokines), ... must be present at a concentration of at least g/L (0.5 g/dL) to be detectable by this method This corresponds to ~10 9 cells producing the antibody Confirmation that such an M component is truly ... synthesized in slight excess, are secreted as free light chains by plasma cells, and are cleared by the kidney, but

Ngày tải lên: 07/07/2014, 04:20

... interaction of cell- surface molecules, termed selectins, on bone marrow endothelial cells with ligands, termed integrins, on early hematopoietic cells Human hematopoietic stem cells can survive ... Hematopoietic Cell Transplantation Hematopoietic cell transplantation can be described according to the relationship between the patient and the donor and by the anatomic source of stem cells In ~1% of ... lymphohematopoietic system, including all red cells, granulocytes, B and T lymphocytes, and platelets, as well as cells comprising the fixed macrophage population, including Kupffer cells of the liver, pulmonary...

Ngày tải lên: 07/07/2014, 04:20

... Appl Phys Lett 72, 16 56 16 58 Han, W., Lindsay, S M., and Jing, T (19 96) A magnetically-driven oscillating probe microscope for operation in liquids Appl Phys Lett 69, 411 1– 411 3 Garcia, R and San ... Cleveland, J P., and Hansma, P K (19 95) Improvement of thermally induced bending of cantilevers used for atomic force microscopy Scanning 17 , 11 7 12 1 Imaging Methods in AFM 13 Imaging Methods in Atomic ... force microscopy Rev Sci Instrum 64, 403–405 10 Meyer, G and Amer, N M (19 88) Novel approach to atomic force microscopy Appl Plrys Lett 53, 10 45 10 47 11 Baselt, D R., Clark, S M., Youngquist, M...

Ngày tải lên: 06/08/2014, 02:20

... 14 , 15 18 15 21 Sheng, S., Czajkowsky, D M., and Shao, Z (19 99) AFM tips: How sharp are they? J Microsc 19 6, 1 5 Artifacts in AFM 37 Taatjes, D J., Quinn, A S., Lewis, M R., and Bovill, E G (19 99) ... Tech 44, 312 –326 10 Dinte, B P., Watson, G S., Dobson, J F., and Myhra, S (19 96) Artefacts in noncontact mode force microscopy: The role of adsorbed moisture Ultramicroscopy 63, 11 5 12 4 11 Yang, ... S329–S332 14 Paredes, J I., Martinez-Alonso, A., and Tascon, J M (2000) Adhesion artefacts in atomic force microscopy imaging J Microsc 200, 10 9 11 3 15 Barrett, R C and Quate, C F (19 91) Optical...

Ngày tải lên: 06/08/2014, 02:20

... York, 14 th, pp 4 01 404 15 Hansen, K M., Ji, H F., Wu, G H., et al (20 01) Cantilever-based optical deflection assay for discrimination of DNA single-nucleotide mismatches Anal Chem 73, 15 67 15 71 16 ... Bioelectronics 17 , 17 3 17 9 Fritz, J., Baller, M K., Lang, H P., et al (2000) Translating biomolecular recognition into nanomechanics Science 288, 316 – 318 Berger, R., Delamarche, E., Lang, H P., et al (19 97) ... needs to be tracked continuously 3.2 AFM Imaging and F-d Analysis 3.2 .1. Imaging of Cells 3.2 .1. 1 FIXED OR DEHYDRATED CELLS When a cell is fixed, through cross-linking of the plasma membrane and/or...

Ngày tải lên: 06/08/2014, 02:20

... high-resolution imaging in cell biology Trends Cell Biol 2, 208– 212 Hong, X and Lei, Y (19 99) Atomic force microscopy of living cells: progress, problems and prospects Methods Cell Sci 21, 1 17 Bushell, G ... Invest Ophthalmol Vis Sci 41, 680–686 16 Meller, D., Peters, K., and Meller, K (19 97) Human cornea and sclera studied by atomic force microscopy Cell Tissue Res 228, 11 1 11 8 Corneal Tissue Observed ... interlinking network throughout the cornea (11 13 ) AFM has been recently introduced with success in the research of corneal surfaces and components (11 ,14 16 ) Compared with other forms of microscopy...

Ngày tải lên: 06/08/2014, 02:20

... example follows: Freq High 12 8. 410 kHz Low 12 5. 410 kHz Gain 1. 000 Vib Voltage 1. 511 V LPF 1. 0 kHz HPF 1. 0 kHz Time 5s Vib Freq 12 6.800 Amplitude 1. 068 V Peak Freq 12 6. 918 kHz ∆F 0.335 Q 379.273 ... Abe 11 Immerse the samples in 3-butyl-ethyl acetate solution for 15 12 Put a sample in a cage and perform critical point drying 3.2 Scanning Operation Method of Dynamic Force Mode (DFM) 10 11 12 ... mice (Fig 1; refs 10 ,11 ) These segments perform different roles in the process of sperm maturation (12 ) Spermatozoa are immature in segment I but mature in segment V (13 ) The epithelial cells in...

Ngày tải lên: 06/08/2014, 02:20

... problems (12 ,16 ,18 ) Since most cells are too large to observe them as a whole (Fig 5), only portions of the cells can be investigated Figs and 8A (pp 11 7, 11 8) show rare examples of cancer cells ... cantilevers are 0. 01 10 0 N/m and instrumental sensitivities for normal deflections are up to approx 0. 01 nm, the corresponding limits in force detection are 10 13 10 –8 N (12 ) Because of their ... Surface Structures With AFM 12 1 Fig 10 AFM observation of adherently growing cells (hypopharynx carcinoma) (16 ) (A) Viable cells investigated in noncontact mode The cells are displayed with an...

Ngày tải lên: 06/08/2014, 02:20

... Press Inc., Totowa, NJ 12 5 12 6 Ricci et al Materials 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Chick embryos (7–8 days (d) old) Chick embryo extract (Gibco 16 460- 016 ) Hanks’ balanced ... 65, 14 9 16 3 14 Bridgman, P C and Dailey M E (19 89) The organization of myosin and actin in rapid frozen nerve growth cones J Cell Biol 10 8, 95 10 9 14 0 Ricci et al 15 Bridgman, P C (19 91) Functional ... Bridgam P C (19 92) Nerve growth cone lamellipodia contain two populations of actin filaments that differ in organization and polarity J Cell Biol 11 9, 12 19 12 43 12 Da-Yu, W and Golberg, D J (19 93) Regulated...

Ngày tải lên: 06/08/2014, 02:20