translating studies in breast cancer models to clinical insights

... observed Initial DNA damage levels in breast cancer patients Radiation-induced apoptosis in breast cancer patients Initial DNA damage was determined as radiationinduced double-strand breaks (DSB) in ... subcutaneous late toxicity in our series This finding opens the possibility to develop new predictor assays taking into account the initial DNA damage and radiation-induced apoptosis levels, and introduces ... and radiation-induced apoptosis with normal tissue toxicity As previously published, increasing numbers of radiation induced DSB were related to severe late toxicity in breast cancer patients...

Ngày tải lên: 09/08/2014, 09:20

... binds to the hypoxiaresponsive element of target genes that encode proteins involved in various cellular processes, including inhibition of apoptosis Hypoxia has been reported to inhibit apoptosis ... Collectively, the data in Fig support the involvement of a TRAIL-dependent process in mediating hypoxiainduced changes in BRCA1 localization TRAIL, but not hypoxia, induces apoptosis in breast cancer cells ... truncated BRCA1 or induce apoptosis in a cell line expressing mutated BRCA1 The data in Figs and suggest that TRAIL can alter BRCA1 localization and induce apoptosis in breast cancer cells Therefore,...

Ngày tải lên: 30/03/2014, 03:20

... levels according to tumor depth (B) IL-6 levels according to LN metastasis (C) IL-6 levels according to the metastasis (D) IL-6 levels according to TNM staging The patients were divided into two groups ... invasion begins with inflammation around cancer cells Thus, it has been reported that serum CRP levels are higher in cases of invasive cancer than in cases of non-invasive cancer [8,9] The principal ... characteristics of breast tumour (A) IL-6 levels according to tumor depth (B) IL-6 levels according to LN metastasis (C) IL-6 levels according to the metastasis (D) IL-6 levels according to TNM staging Ravishankaran...

Ngày tải lên: 09/08/2014, 01:24

... washings, sections were incubated for 30 in the secondary biotinylated antibody (Multilink Swine anti-goat/ mouse/rabbit immunoglobulin; Dako Inc.) Following washings, Avidin Biotin Complex (Vector ... relationship of E-cadherin, α-catenin, β-catenin and γ-catenin in breast tumours to Nottingham Prognostic Index (NPI) (E-cadherin p = 0.094, αcatenin p = 0.144, β-catenin p = 0.378, γ-catenin p = 0.131) ... http://www.wjso.com/content/6/1/56 were incubated in the dark for minutes Sections were then counterstained in Mayer's haematoxylin and dehydrated in ascending grades of methanol before clearing in xylene and mounting under...

Ngày tải lên: 09/08/2014, 07:21

... patients, multiple routes leading to a single SN in 4%, a single route leading to multiple SNs in 10% and multiple routes leading to multiple SNs in 8% These results are in agreement with those reported ... Statman R: Sentinel lymphadenectomy in breast cancer J Clin Oncol 1997, 15:2345-2350 Minato M, Hirose C, Sasa M, Nishitani H, Hori A, Morimoto T: Axillary 3D CT imaging with lymphoscintigraphy is ... comparing radical mastectomy, total mastectomy, and total mastectomy followed by irradiation N Engl J Med 2002, 347:567-575 Giuliano AE: Sentinel lymphadenectomy in primary breast carcinoma:...

Ngày tải lên: 09/08/2014, 07:21

... important domains such as 1) the C-terminal protein kinase domain (PI3K-domain), 2) the substrate binding domain in the N-terminal of the protein necessary for activation of p53 in response to DNA damage, ... PI3K domain, substrate binding domains and ATPbinding domains, is illustrated in Figure Single marker associations Risk for developing breast cancer The association between any variant in the ATM ... binding Leucine zipper Val-82 to Ser-89 Val-1218 to Leu-1238 Exon Proline rich FAT domain Asp-1373 to Pro-1382 ATM protein NO controls NO BC American BC (± IBTR) Ser-1966 to Ala-2566 ATP-binding...

Ngày tải lên: 09/08/2014, 10:21

... Tissues were incubated in 10X penicillin/streptomycin/neomycin, minced in DMEM/F12 containing 1X penicillin/streptomycin/neomycin, 10% FBS, 10 μg/ml insulin, μg/ml fungizone, 100U/ml hyaluronidase ... penicillin/streptomycin, penicillin/streptomycin/neomycin, fungizone, Cyquant, Xgal, Alexa-Fluor antibodies (Invitrogen); soybean trypsin inhibitor, collagenase I, hyaluronidase 1-S, DMEM/ Ham’s F12, bovine ... marker studies can yield important insights into clinical samples Conclusions We have reported reduced senescence in tumour versus non-tumour breast primary cultures, and stepwise increases in the...

Ngày tải lên: 10/08/2014, 10:21

... fibronectin Other ligands, such as collagen and laminin are known to bind to α1, α2, α10 and α11 subunits of β1 integrin family that contain the αA-domain Laminin is also found to bind to non-αA-domain ... Similarly, integrin α4β1 interacts with VCAM-1 to mediate adhesion of renal cancer cells 64 and melanoma cells 65 to endothelium 1.2.4 Integrins in cancer Due to the signalling effects, integrins have ... and intrinsic, linked inflammation and cancer First, in the extrinsic pathway, inflammatory conditions in the body, as a consequence of disease or chronic infection, can increase the risk of cancer...

Ngày tải lên: 09/09/2015, 08:17

... laboratory to be a novel breast cancer metastasis-associated protein TRPV4 was found to be upregulated in invasive breast cancer cell lines and tumor breast tissues It has been shown that 4α-PDD induced ... carboxyl-terminal tail as illustrated in Figure 1.5C appears to be the docking site for at least two interacting sites including calmodulin (CAM) binding sites and region involved in microfilament-associated ... phosphatidylinositol-3,5-bisphosphate phosphatidylinositol 3-kinase phosphatidylinositol 3-phosphate phosphatidylinositol-4,5-bisphosphate phosphatidylinositol-3,4,5-trisphosphate protein kinase C phospholipase...

Ngày tải lên: 02/10/2015, 17:15

... MMP mTOR NF-κB PAK1 PALS1 Atypical protein kinase C Adenosine triphosphate Basic helix-loop-helix Binding protein Bovine serum albumin Cyclin-dependent kinase inhibitor 2B Cytokeratin-19 Cytoplasmic ... Transforming growth factor-β Transforming growth factor-β receptor Unidentified liver spot-35 unfolded protein response Upstream transcription factor X-box binding protein Zinc finger E-box binding ... protein-29 (ERp29) is a chaperone protein that functions in the unfolding and escort of secretory proteins Like other reticuloplasmins, ERp29 is believed to be involved in carcinogenesis In breast...

Ngày tải lên: 13/10/2015, 15:55

... age Clinical breast examination in primary care Each primary care team should include at least one practitioner who has had specific training in carrying out clinical breast examination (CBE) in ... with breast symptoms Women with symptoms which could be due to breast cancer should be referred to the breast care team Routine breast examination, including self-examination, for asymptomatic ... patients to maintain their independence and autonomy C Evidence Minimising delay Although relatively short delays are unlikely to affect the clinical course of the disease, the importance of minimising...

Ngày tải lên: 14/02/2014, 22:20

... screening should be established in line with the routine screening interval, taking into consideration that most programmes not succeed in keeping the routine screening interval for each individual ... screening should be established in line with the routine screening interval, taking into consideration that most programmes not succeed in keeping the routine screening interval for each individual ... ability to progress as one with continuing advances in technical and professional knowledge Certain principles remain just as important in diagnosis as they are in screening Training, multidisciplinary...

Ngày tải lên: 15/03/2014, 00:20

... National Cancer Institute (NCI) This is a pilot study intended to explore psychosocial factors and quality of life, preparatory to embarking on a larger scale, longterm study Finally, we discuss clinical ... threat to a woman’s self-image In addition, as the rate of breast cancer has increased in younger women, issues of femininity arising from the impact of disease on fertility are increasingly important ... take into account all of the complex factors involved in cancer The current literature lends support to the argument that a broader, more integrative framework, which includes psychosocial factors,...

Ngày tải lên: 22/03/2014, 17:20

... growth of breast cancer cells, to trigger the apoptotic signaling in breast cancers, thus switching the estrogenic hormones from potential mitogens to apoptosis inducers in breast cancers (Shi, ... efficient in apoptosis induction DNA-damaging agents like doxorubicin, etoposide, cisplatin or bleomycin may induce apoptosis via both extrinsic and intrinsic apoptotic pathways (Fulda & Debatin, 2006) ... domain originating from a protein toxin, Pseudomonas Exotoxin A (PEA), was embedded in the fusion protein to mediate a translocation of the apoptotic proteins from an intracellular vesicle to...

Ngày tải lên: 23/03/2014, 17:20

... progestin administration was thus associated with the beginning of growth inhibition by progestins GPR30 mRNA is regulated according to the dose of progestin, which also correlated with progestin-induced ... and C-C chemokine receptors Chemokines [27,28] and angiotensin II [29] have both shown growth-inhibiting properties with different cancers Interestingly, an orphan receptor (GPR41 in the rat), ... growth factors and steroid hormones Progestin administration causes an initial increase in cell proliferation lasting about 12–24 h and subsequent GPR30 and progestin (Eur J Biochem 269) 2489 inhibition...

Ngày tải lên: 24/03/2014, 00:21

... findings from the second phase of this study suggest that interventions to reduce delay behavior in help-seeking for breast symptoms should inform women of the diversity of breast cancer symptoms ... Fabbrocini G et al.: Assessment of delay in obtaining definitive breast cancer treatment in southern Italy Breast Cancer Res & Treat 2001, 66:209-215 Montazeri A, Haji-Mahmoodi M and Jarvandi S: Breast ... avoided if patients with breast cancer presented to a doctor earlier [28] Finally, it is worth noting that there were several limitations inherent in this study and the findings cannot be generalized...

Ngày tải lên: 28/03/2014, 14:20

... currently in clinical trials Based on results obtained in preclinical studies with animal models, which showed that systemic administration of growth factor inhibitors could restrain the growth ... bladder by oral administration of the epidermal growth factor receptor protein tyrosine kinase inhibitor 4,5-dianilinophthalimide Clin Cancer Res 3, 161–168 68 Bellmunt J, Hussain M & Dinney CP (2003) ... predictive indicators in primary breast cancer to improve patient selection for specific and individual treatments Moreover, it is important to develop new diagnostic methods to detect breast cancer...

Ngày tải lên: 30/03/2014, 15:20

... al.: Anti-colony-stimulating factor-1 antibody staining in primary breast adenocarcinomas correlates with marked inflammatory cell infiltrates and prognosis J Natl Cancer Inst 1994, 86:120-126 Tang ... adaptive and innate immune cells during breast cancer progression Breast Cancer Res 2007, 9:212 Balkwill F, Charles KA, Mantovani A: Smoldering and polarized inflammation in the initiation and ... p-value less than 0.05 Injection of free DiD resulted in no increase in fluorescent intensity within the tumor at any time point post-infusion Figure labeling (a) Optical imaging of DiD-labeled cells...

Ngày tải lên: 18/06/2014, 15:20

... measurement in randomized clinical trials in breast cancer – taking stock Journal of the National Cancer Institute 2003, 95(4):263-281 Montazeri A: Health-related quality of life in breast cancer ... international clinical trials in oncology J Natl Cancer Inst 1993, 85(5):365-376 Bottomley A, Aaronson NK: International perspective on health-related quality-of-life research in cancer clinical trials: ... oncology clinical research [29-31] It has also been validated [32] and found to be responsive [33] specifically in breast cancer patients and is widely used in breast cancer research investigating...

Ngày tải lên: 18/06/2014, 19:20

... growth of breast cancer cells, to trigger the apoptotic signaling in breast cancers, thus switching the estrogenic hormones from potential mitogens to apoptosis inducers in breast cancers (Shi, ... efficient in apoptosis induction DNA-damaging agents like doxorubicin, etoposide, cisplatin or bleomycin may induce apoptosis via both extrinsic and intrinsic apoptotic pathways (Fulda & Debatin, 2006) ... domain originating from a protein toxin, Pseudomonas Exotoxin A (PEA), was embedded in the fusion protein to mediate a translocation of the apoptotic proteins from an intracellular vesicle to...

Ngày tải lên: 27/06/2014, 11:20