trained by ofis on clinical cases

... different culture conditions The open bars in the graphs show the proportions of conditions neurones (%) in the indicated size ranges; the black insets show the proportions of neurones that express ... therefore, it is difficult to make firm conclusions about whether FLS cells exert trophic influences on neurones under non-inflammatory conditions However, comparison of the effects of FLS cells from ... that the expression of NK1 receptor-like IR in DRG neurones is upregulated by long-term addition of either PGE2 [43] or IL-6 [46] to the culture medium, we measured the concentration of PGE2 and...

Ngày tải lên: 09/08/2014, 10:20

... injection within one hour [8] Additionally, long term potentiation (LTP) was rapidly induced by afferent stimulation and lasted more than one hour in VTA slice [9] Moreover, a positive correlation ... provides more information than traditional measures -9- based on only peak ratios Specifically, as mentioned in the method section, we estimated areas under AMPA and NMDA curves on consecutive 50ms ... potentiation in dopamine neurons Nature 2001, 411: 583-587 Saal D, Dong Y, Bonci A, Malenka RC: Drugs of abuse and stress trigger a common synaptic adaptation in dopamine neurons Neuron 2003, 37: 577- 582...

Ngày tải lên: 19/06/2014, 08:20

... infection in the absence of CD4 recognition by gp120 projecting from the envelope of HIV virions [34-36] Additional interactions between virions and mucosal surfaces may be supported by host ... HIV infection in vitro [31,50] A detailed evaluation by fluorescence activated cell sorting (FACS) of co-receptor expression on tonsil cell suspensions provided valuable information relating ... suspensions from tonsil were prepared by forcing the tissue through a metal tissue sieve Viable mononuclear cells were further purified by banding on a ficoll gradient and then mixed cell populations,...

Ngày tải lên: 20/06/2014, 01:20

... progesterone receptors could be detected in any of our cases by means of well known immunohistochemical method using well documented monoclonal antibodies (Figure 2) In the control Figure demonstrable ... Labit-Bouvier et al 2000 69 none 10 10 Cafer et al 2008 59 none 100 11 Dalgorf et al 2008 none none 12 Jaiswal et al 2009 100 none none Page of (page number not for citation purposes) Journal of Negative ... retrospectively determine the expression of estrogen and progesterone receptors by means of immunohistochemical methods using monoclonal antibodies in 100 cases of vestibular schwannomas and also...

Ngày tải lên: 11/08/2014, 07:21

... production by cotton rat transfectant cells after HIV-1 infection Next, we infected the engineered cotton rat CD4 clones to define the time course of p24gag production (Fig 2) When the CCRT K4 clone ... isolation infected ratDetection of episomalsequence from HIV-1of cottoncotton Detection of episomal cDNA and isolation of cotton rat-HIV-1 chimeric DNA sequence from HIV-1 infected cotton rat ... and bone marrow) from infected cotton rats The virus induced a strong, specific and long-lasting immune response that was maintained up to year post-infection Although not demonstrated by direct...

Ngày tải lên: 12/08/2014, 04:21

... production by cotton rat transfectant cells after HIV-1 infection Next, we infected the engineered cotton rat CD4 clones to define the time course of p24gag production (Fig 2) When the CCRT K4 clone ... isolation infected ratDetection of episomalsequence from HIV-1of cottoncotton Detection of episomal cDNA and isolation of cotton rat-HIV-1 chimeric DNA sequence from HIV-1 infected cotton rat ... and bone marrow) from infected cotton rats The virus induced a strong, specific and long-lasting immune response that was maintained up to year post-infection Although not demonstrated by direct...

Ngày tải lên: 12/08/2014, 04:21

... among the groups (Figure 2b) The expression and distribution of VEGF in the lung tissues were examined with IHC VEGF expression in control and sham-operated animals was characterized by strong ... had no effects on cell viability and protein levels of VEGF and VEGFR1 in comparison with non-treated control Discussion The present study is a comprehensive report on the early responses of VEGF ... correlation studies are small; thus, these results should be interpreted with caution We noted a significant correlation of the VEGF concentrations with the total protein concentrations and with...

Ngày tải lên: 13/08/2014, 03:20

... neurons This may suggest a dual mechanism by glutamatergic input, one to inhibit NSPC proliferation and the other to maintain cell survival during neuronal maturation Upon migration to the region ... the rate of proliferation Multipotent Differentiation after Vitrification 2.4 Discussion 81 SEROTONERGIC FIBRES AND 5-HT IMPLICATIONS FOR NSPC PROLIFERATION 3.1 Introduction 92 3.2 3.2.1 3.2.2 ... the likelihood of direct serotonergic control of NSPC proliferation The notion of direct serotonergic control was further reinforced by findings that exogenous addition of 5-HT to cultured NSPCs...

Ngày tải lên: 11/09/2015, 10:15

... ice for additional 30 As a baseline control one tray was left on ice with LiCl incubation buffer without agonist The EC50 was determined by adding escalating concentrations of agonist (10)12 to ... Discussion Phosphorylation of serine or threonine residues in the C-terminus of GPCRs by second messenger kinases or specific GRKs is a requirement for receptor sequestration [23] However, the context ... experimental conditions, an amphipathic a-helix was formed in this region [31] By contrast, NMR studies of peptides representing the same region of rhodopsin in membrane and detergent-free solutions displayed...

Ngày tải lên: 07/03/2014, 16:20

... khauvinhcong@gmail.com k khauvinhcong@gmail.com khauvinhcong@gmail.com khauvinhcong@gmail.com ...

Ngày tải lên: 12/11/2013, 13:52

... described by a two-exponential decay phase model, whereas dissociation at low pH was best described by a one-exponential decay phase model Final concentrations of unlabeled ligands in the dissociation ... regression analyses of binding data gave dissociation constants (Kd), calculated from the reciprocal of the slopes Association data were fitted by either the one-phase exponential association equation ... association curves were well fitted by a twophase exponential association model, suggesting that surface binding has two components, one rapid and one slow The half-time for association of [125I]TCPCSK9...

Ngày tải lên: 14/02/2014, 14:20

... Accession numbers The NRs transcriptionally controlled by 20E, in an in vitro fat body culture system are indicated in the 20E response column NT, not tested; P, primary response; S, secondary response; ... termination of Vg expression [17], but the mechanism is still unknown The regulation of AaVg gene expression by 20E acts not only through members of the NR family, but also through other transcription ... and DmHR4 function as a switch that defines the larval–prepupal transition by arresting the early regulatory response to ecdysone at puparium formation, thus facilitating the induction of the DmbFTZF1...

Ngày tải lên: 18/02/2014, 13:20

... at high stringency Two BAC clones that hybridized strongly were later confirmed to be true positives by Southern hybridizations Direct sequencing on one of the BAC clones yielded the 3¢ and 5¢ ends ... polymerase, on an ABI PRISM 310 Genetic Analyzer, and found to be identical to the genomic sequence The expression construct contains one upstream in-frame methionine codon (immediately after the cloning ... thereby making it unlikely that cNPY would bind with higher affinity (they differ by only two conservative replacements as mentioned above, see Fig 10) Another observation in the same direction...

Ngày tải lên: 19/02/2014, 07:20

... concentration of 25 nM The dissociation constants were obtained by fitting the binding curves using the computer program ORIGINTM 6.0 (Northampton, MA, USA) based on the following equations: Kon ... removed by centrifugation and the supernatant was concentrated by ultrafiltration (Millipore, Bedford, MA, USA) The protein concentration was determined at A280 with a calculated extinction coefficient ... subcloned into the pGFN vector All these expression constructs were confirmed by DNA sequencing and transformed into the E coli BL21(DE3) expression host Protein expression and purification The...

Ngày tải lên: 19/02/2014, 12:20

... different conditions for separation of the samples As shown in Fig 2, samples were separated by centrifugation at 4500 g for min, centrifugation at 12 000 g for 10 min, or by using filtration (0.2-lm ... be caused by conformational changes in the proteins rather than changes in protein– protein interaction A combination of several methods seems therefore important for the demonstration of GPCR ... used by these authors, i.e the fusion of the receptor to a fluorescent protein, may have affected the conformation of these receptors Indeed agonist dose–response curves for the stimulation of...

Ngày tải lên: 21/02/2014, 00:20

... corresponding sequencer runs Electron microscopy of rotary shadowed proteins [34], CD, and matrix-assisted laser desorption ionization MS [35] followed standard protocols Construction of expression ... responsible for dimerization The native b1IV dimer was therefore ®rst modi®ed by partial reduction and pyridylethylation to protect the thiol followed by complete reduction under denaturing conditions ... ABC (Fig 2) and A,C but not by chondroitinase B or heparitinase (not shown) yielded the b1IV monomer and dimer bands, demonstrating the exclusive substitution by chondroitin sulfate The mean (SD)...

Ngày tải lên: 21/02/2014, 03:20

... detection was monitored at 214 nm Peptide purity was assessed using an analytical Vydac C18 column (250 · 0.46 mm) using the same elution conditions Disulfide bond formation and protein purification ... stimulation of isolated mouse hemidiaphragms, previously treated with formamide (to uncouple excitation-contraction coupling), elicited action potentials at junctional areas without contraction triggered ... by endplate potentials Addition of lM sWntx-5 to the standard solution did not block neuromuscular transmission, even after 30-min incubation In contrast, the potent a-cobratoxin used as a control,...

Ngày tải lên: 21/02/2014, 03:20

... peptide elongation, or coupling, was monitored by using a ninhydrin assay to check for residual free a-amine To facilitate coupling of difficult residues, combinations of the following conditions were ... Disulfide bond formation was achieved by Ó FEBS 2004 1750 T Sprules et al (Eur J Biochem 271) stirring the reaction mixture with elemental iodine (0.1 M in methanol) under argon for h The oxidation was ... protocol The fractions were analyzed by MALDITOF MS and also by SDS/PAGE (12%) to determine if they contained the expected MBP-PreCbnB2 fusion protein The appropriate fractions were combined and...

Ngày tải lên: 07/03/2014, 15:20

... reduction and alkylation produced only one band in SDS/PAGE with the expected molecular mass of the monomer Considering that dimerization could occlude the TCR binding site, we also constructed ... Vb5.2 concentrationdependent binding to both SSA species was observed The association rate constant (kass) was too fast to be accurately measured On the contrary, the dissociation rate constant ... Trials using higher concentrations were unsuccessful due to nonspecific aggregation of the Vb2.1 TCR Discussion Fig Dose-dependent T-cell proliferation by the different SAg preparations As indicated...

Ngày tải lên: 07/03/2014, 16:20

... count after incubation in the odorant dilutions, and also by monitoring the influence of odorant dilutions on the luciferase bioassay performed with S14 stimulation of a control yeast strain coexpressing ... final odorant concentration of · 10)5 m corresponds to a concomitant dilution of the organic solvent that is not deleterious to the yeast cells This was tested by cell shape examination and count ... response to odorant stimulation The yeast system was chosen for several reasons Firstly, S cerevisiae has been successfully used for functional expression of many GPCRs [22–28] Secondly, yeast constitutes...

Ngày tải lên: 07/03/2014, 16:20