tph or restoration of the lost activity of tph

... Cavitation: The formation and collapse of gas pockets or bubbles on the blade of an impeller or the gate of a valve; collapse of these pockets or bubbles drives water with such force that it ... Available Chlorine: A measure of the amount of chlorine available in chlorinated lime, hypochlorite compounds, and other materials used as a source of chlorine when compared with that of liquid or gaseous ... Confidential Statement of Formula (CSF): A list of the ingredients in a new pesticide or chemical formulation The list is submitted at the time for application for registration or change in formulation B...

Ngày tải lên: 24/04/2013, 14:40

... presence of the JNK inhibitor SP600125 or in control conditions (Fig 3C) Although the presence of the inhibitor had no significant effect on cell survival or caspase activity in control cells or after ... version of Itch together with Bid or tBid fused to green fluorescent protein (GFP) at the C-terminus We then immunoprecipitated FLAG–Itch and looked for the presence of GFP fusions in the immunoprecipitated ... required for many of the morphological changes that occur during apoptosis The mitochondrial release of cytochrome c and second mitochondria-derived activator of caspase (Smac ⁄ DIABLO) allows for the...

Ngày tải lên: 16/02/2014, 09:20

Ngày tải lên: 16/02/2014, 15:20

... report that the effect of these additives on the chaperone action of aB-crystallin is dependent on the target protein used, and therefore the results highlight the need to assess the activity of ... 7.4, for days either in (A) the absence or (B) the presence of aB-crystallin (0.4 mgÆmL)1) and either 250 mM of Gly (d), 250 mM of Lys-HCl ()), 250 mM of Arg-HCl ( ) or 100 mM of GdnHCl (h) The ... of the effect of these concentrations on the chaperone activity of aB-crystallin (Fig 4B,C) None of the compounds had an effect on the morphol- A C 940 B ogy of the amyloid fibrils formed (data...

Ngày tải lên: 18/02/2014, 16:20

... (a) the model of the membrane was oriented with each layer parallel to the xy plane so that all the phosphorus atoms of each layer had the same z coordinate and the z axis was perpendicular to the ... the center of the membrane (Fig 4); (b) the major axis of the dimeric RNase was made coincident with the z axis of the system, hence orthogonal to the membrane plane (Fig 4); (c) the dimeric ... to the attraction of the RNase to a membrane, as evidenced by the RNase negative EIEmax values As a measure of the RNase cytotoxicity we used the reciprocal of the IC50 parameter The values of...

Ngày tải lên: 19/02/2014, 06:20

... inhibitor for the binding of enzyme This theoretical inhibition was significantly higher than the experimental one, again confirming the above hypothesis The most important differences were found for the ... dissociation of the inhibitory complexes (Ki ¼ kdiss ⁄ kass) The complexes formed of native elafin or trappin and NE or Pr3 have similar kdiss values, which correspond to a half-life of dissociation of ... binding of the methionine sulfoxide residue at the S1¢ subsite of the active centers of NE and Pr3 The fact that the S1¢ subsite of Pr3 is significantly smaller than that of NE [21] might then explain...

Ngày tải lên: 19/02/2014, 07:20

... more than the catalytic activity of RTA R213D The X-ray structure of RTA R213D In the absence of structural data for each of the RTA variants discussed, it is possible that the reduction in activity ... between the base G4 and the sidechain of Asn122 In the second model (Fig 1B) the structure of the hexanucelotide is, on the whole, unchanged and the majority of the interactions that were seen in the ... different effects on the activity of RTA Whereas, one had no effect on the activity of the enzyme (R258A), others reduced activity by one (R213A, R258D, R48A) or by two (R213D) orders of Ó FEBS 2003...

Ngày tải lên: 19/02/2014, 12:20

... F is the normalized uorescence, [P] is the concentration of TNP-ATP, [E] is the concentration of the enzyme, c is the enhancement of uorescence intensity of the bound probe relative to the free ... component of q1 ẳ 11.3 ns and a shorter component of q2 ẳ 1.2 ns, seemed to satisfactorily t the collected data The shorter component is short enough to be ascribed to the wobbling of the uorophore ... due the formation of an hydrogen bond of the NO2-group of pNPP with the amino group of N398 The hydrophobicity of the binding pocket is achieved by W411 and L414 The phosphate group seems to form...

Ngày tải lên: 19/02/2014, 16:20

... that the oxygen atom of the northern ribose is indeed important for Ca2+ release [56] This situation is unique for the northern ribose since replacement of the oxygen by a carbocyclic bridge in the ... expression of either cytosolic or membrane-bound enzymes not related to CD38 or CD157 [18,27–31] None of these enzymes have been identified on the molecular level so far; however, some (or all) of them ... increases of [Ca2+]i can Second messenger function of cADPR be achieved by increasing the open probability of Ca2+ channels, either localized in the membranes of intracellular Ca2+ stores or in the...

Ngày tải lên: 20/02/2014, 01:20

... study therefore, we investigated how O2 modulates the expression of mRNAs for CytOX subunits and also the catalytic activity of the enzyme complex Our results show that the levels of some of the ... and alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes ... approximately correspond to 0.1 and 21% of oxygen, respectively Normally the cells were exposed to either normoxic or hypoxic conditions for a period of 6–12 h In each experiment, a group of cells...

Ngày tải lên: 20/02/2014, 23:20

... distortion of the porphyrin ring and a shift of the iron atom outside the plane of the porphyrin Binding of imidazole to the iron(III) of Fe(ToCPP), a,a-1,2- a,b-1,2-Fe(DoCPP), Fe(MoCPP) and to their ... of the porphyrin and, in the more favorable conformation, H2O2 binds to the iron on the same face of the porphyrin as the catalytic COOH residue, the imidazole binding to the iron on the opposite ... in the case of Fe(ToCPP) (2.70 ‹ 0.04 mM) (Table 2), that even the formation of the mono-imidazole complex of 13G10±Fe(ToCPP) is more dif®cult than the formation of the bis-imidazole complex of...

Ngày tải lên: 21/02/2014, 03:20

... factor of the Ets-family is involved in the regulation The members of the Ets-family of transcription factors bind to specific purine-rich sequences with a core motif of GGAA/T and control the ... inserted into the KpnI/SacI site of Fig Structures of the 5¢-flanking region of mouse a3 integrin gene The map (upper line) shows the organization of exons 1–3 and the 5¢-flanking region with the positions ... IV [57] The former enzyme is responsible for the synthesis of the b1–6 branch in N-acetyllactosamine units in cell surface N-glycans, and the latter enzyme is involved in the synthesis of cell...

Ngày tải lên: 21/02/2014, 03:20

... of the original activity after h The activity of N263D-Sendai decreased to 45% and 10% of the original after and h of incubation at pH 12, respectively N263D/A266Y-Sendai showed only 10% of the ... enzyme showed 63% of the original activity after h of incubation However, the single mutation Tyr269Ala showed only 1% of the original activity after alkaline treatment for h The other substituted ... wild-type ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability of the structure of the protein The difference of the increments...

Ngày tải lên: 21/02/2014, 03:20

... gives the readers of the journal the sort of words and the sort of thoughts they are used to—so, on a larger scale, the writers of an age, without thinking of it, give to the readers of the age the ... of any theory as to the nature of matter, or the nature of mind They are as true upon the theory that mind acts on matter—though separate and altogether different from it—as upon the theory of ... view of the primeval condition of the human race which is known as the Patriarchal Theory There is no doubt, of course, that this theory was originally based on the Scriptural history of the Hebrew...

Ngày tải lên: 06/03/2014, 13:20

... cleavage of the c-phosphate does occur in the NBD with the mutation at the IC [36,38,39] Moreover, these and other studies suggest that the IC residues are involved in the formation of the NBD ... represent < 1% of the activity of the wild-type enzyme We then determined, by photoaffinity labeling, whether any of the mutations altered the apparent binding affinity of Abcb1a for ATP Purified ... around the b-phosphate of bound nucleotide [22], the Walker B motif is responsible for coordinating the essential Mg2+ cofactor [22,24,25], the signature sequence contacts the c-phosphate of the...

Ngày tải lên: 07/03/2014, 06:20

... that phosphorylation of these sites might affect the DNA binding and /or dimerization activities of dHAND We first analyzed the effect of phosphorylation on the transcriptional activity of dHAND ... phosphorylation form, showed weak transcriptional activity (Fig 6B) The expression level of dHAND-Asp-X was lower than those of others in the absence of E47 But in the presence of E47, the expression ... Phosphorylation of dHAND did not affect the dimerization activity with E47 To clarify the mechanisms of the inhibition of dHAND activity by Akt phosphorylation, we examined whether dimerization activity...

Ngày tải lên: 07/03/2014, 16:20

... while the N-terminus served as the pharmacophore and hence the interactive domain with the bacterial target protein, the C-terminal half aided the delivery of the pharmacophore into the interior of ... pyrrhocoricin is in the order of 50 lM [6], is expected to be lower for the full-sized protein, and correlates well with the 5–10 lM antibacterial activity of the same fluoresceinlabeled pyrrhocoricin ... representing the unordered structure suggests the generation of a more ordered conformation Identification of the pyrrhocoricin binding surface on E coli DnaK Encouraged by the success of the Ala-scan...

Ngày tải lên: 08/03/2014, 10:20

... part account for differences in the IC50 values for bacitracin-mediated inhibition of PDI reductive activity Furthermore, on the basis of our results, the inhibitory activity of the bacitracin ... with the 95% confidence level range in parentheses inhibited 90% of the activity Smith et al [22] reported an IC50 value of 152 lm for bacitracin for the inhibition of PDI reductive activity The ... diminish the immunoblot signals for bacitracin at the site of PDI (Fig 4C) These data support the view of a robust covalent PDI–bacitracin interaction We hypothesized that the thiazoline ring of bacitracin...

Ngày tải lên: 14/03/2014, 23:20

... Lenders accept or reject the contract and choose whether or not to monitor Borrowers of type S then choose their action Given these assumptions, there is no signaling or other motive for borrowers ... since there is no benefit from monitoring types G and B The higher the cost of monitoring, the larger is the share of borrowers of type S needed in order for monitoring to occur B Monitoring does ... analyze these issues, I apply a theory of the firm’s choice of debt instrument from the corporate finance literature to the case of emerging market debt The model suggests that the choice of debt...

Ngày tải lên: 15/03/2014, 07:20

... cofactors We therefore characterized the effect of GPR30 on the expression of receptors and cofactors In our preliminary analysis, GPR30 affected the expression level of TIF2 mRNA, but not of ... of other cofactors (nuclear receptor corepressor, silencing mediator of retinoic acid and thyroid hormone receptor, p300/CBP-associated factor, Ras-related C3 botulinum toxin substrate 3) or receptors ... transcriptional activity of the glucocorticoid response element in hTERT-HME cells The finding that GPR30 down-regulated the expression of cofactor TIF2 led us to study whether GPR30 affects the transcriptional...

Ngày tải lên: 16/03/2014, 18:20