topics in nonlinear functional analysis

Ngày tải lên: 21/09/2013, 15:11

Ngày tải lên: 17/02/2014, 20:20

... N-terminal region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o f BMAL1 in a mammalian two-hybrid assay. Accordingly, a Fig. 5. Analysis of DEC1 mutants for binding to ... by DEC1 coincided with that for interaction with BMAL1 and binding to the E-boxes, indicating the importance of t his region in DEC1 for its suppressive activity against CLOCK/BMAL1- induced transcription. Materials ... Arg65, is responsible for t he E-box binding. Determination of the region in BMAL1 for binding to DEC1 To identify the region in BMAL1 required for the binding to DEC1, w e constructed e xpression...

Ngày tải lên: 19/02/2014, 16:20

... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out with myc antibody using detergent-soluble cell lysates containing the amount of protein indicated. ... protein. Tubulin serves as a loading control. The relative amount of mutant protein was calculated based on the ratio of the band intensities of myc-GALE to tubulin in the western blot and indicated ... measuring the intensity of the individual GALE and tubulin bands in the western blot using Scion Image (Scion Corporation, Frederick, MD, USA). Generation of stable cell lines and BrdU incorporation...

Ngày tải lên: 07/03/2014, 00:20

... also affects the binding of galactose at some point in the reaction. Modest changes in K m,ATP are seen in H44Y and R68C. That H44 in uences the binding of both substrates suggests that the binding sites ... change in the enzyme, which creates a functional binding site for galactose. Identifying the nature of this change and the residues involved in transmitting information through the protein will ... 2003 homoserine kinase, proton transfer is unlikely to play a major role in the rate-determining step of catalysis. Several of the disease-causing mutations are not soluble following induction in E....

Ngày tải lên: 08/03/2014, 02:20

... terpenes are produced in resin ducts [2,3]. Their biosynthesis is induced upon wounding [4–6], indicating their role in the defence against bark beetle infestation. For angiosperms many investigations ... after drying dissolved in 5 m M Tris, pH 6.8, 0.2% (w/v) SDS and 1% glycerol. Protein concentration was determined using the BCA Protein assay kit using BSA as protein standard reference, according ... to 200 °Cat5°CÆmin )1 with He gas flow at 3 mLÆmin )1 . The compounds of interest were transferred from GC1 to GC2 from 6.6 min to 7.1 min (a-pinene) and 10.2 min to 10.4 min (b-pinene). The fused silica...

Ngày tải lên: 08/03/2014, 23:20

... DNA-binding protein of the ferritin family 563 nt 20 052 Da Contains domains typical of the ferritin-like superfamily, such as: DNA-binding ferritin- like protein, ferritins and bacterioferritins Putative ... shows an increased level in the dmdR1 mutant, is a DNA-binding protein with domains typical of the ferritin superfamily. This protein might be involved in a cascade of iron regu- lation in response ... available regarding ferritin regulation in Streptomyces species. In addition to the 11 proteins listed in Tables 1 and 2, minor changes in other proteins were observed. These proteins may be involved in other...

Ngày tải lên: 16/03/2014, 18:20

... °Cor 22 °C), by using an alternative expression host {BL21(DE3)[pLysS]} or by including protease inhibi- tors in the solutions used during purification. Kinetic analysis of disease-causing mutations The ... these enzymes and inserted into the corresponding sites in the expression vector pET-21d (Merck, Nottingham, UK). The entire GALE coding sequence was verified by DNA sequen- cing. The recombinant plasmid ... stained with Coomassie blue. C, control lanes containing 50 l M GALE; P, GALE digested with 90 n M thermolysin for 30 min at 37 °C; U, GALE digested with 90 n M thermolysin for 30 min at 37 °C in...

Ngày tải lên: 23/03/2014, 11:20

... similarity to human uridine kinase, UMPK, and UDPK. Uridine kinases are downstream of UP in the pyrimidine salvage pathway. Homology searches revealed that several uridine kinases exist in the C. ele- gans ... is expressed in the hypo- dermis and intestine, both UP ⁄ UMPK in the hypo- dermis and OPRT ⁄ UMPK in the intestine are essential for mediating 5-FU function in C. elegans. It is also possible ... a control. In vitro enzymatic assays To induce the MBP–UPP1 fusion protein, 0.3 lm IPTG was added to the culture. Induced fusion proteins were purified using amylose resin (New England Biolabs), according...

Ngày tải lên: 30/03/2014, 01:20

... and there exist nonlinear terms in the objective function and constraints, then (1.1) is a mixed-integer nonlinear programming MINLP problem. The fundamentals of MINLP optimization ... multipliers in a constrained nonlinear optimization problem have a similar inter- pretation to the dual variables or shadow prices in linear programming. To provide such an interpretation, ... stationary points of the Lagrange function correspond to the minimum of (3.3). Illustration 3.2.2 Consider the following two-variable constrained nonlinear programming prob- lem in the ...

Ngày tải lên: 01/04/2014, 11:35

... ∞)and  ∞ t 0 1 ψ(φ −1 (s)) ds = ∞ , a(t), a(t) are defined as in Theorem 2.1, and a(0) > t 0 >0. If x Î C(R + , R + ) satisfies the following integral inequality containing multiple integrals φ(x(t)) ≤ a(t)+  α 1 (t) 0 f ... R + ), and a, j, ψ , ω, a, b, J, Y are defined as in Theorem 2.3. If u Î C(R + × R + , R + ) satisfies the following inte- gral inequality containing multiple integrals φ(u(x, y)) ≤a(x, y)+  β ( y ) 0  α ( x ) 0 [f ... R + ), i =1,2,anda, j, ψ, ω, J, Y are defined as in Theorem 2.3. If u Î C(R + × R + , R + ) satisfies the following integral inequality containing multiple integrals φ(u(x, y)) ≤ a(x, y)+  β ( y ) 0  α ( x ) 0 [f 1 (s,...

Ngày tải lên: 21/06/2014, 01:20

... 11T99 Abstract The main purpose of this paper is to show that many inequalities in functional analysis, probability theory and combinatorics are immediate corollaries of the best approximation theorem in inner ... of combinatorics 17 (2010), #R58 4 Proof. Since the total number s of (n − 1)-dimensional hyperplanes passing through the origin equals the total number of lines passing through the origin, s ... electronic journal of combinatorics 17 (2010), #R58 3 The main purpose of this paper is to show that many inequalities in functional analysis, probability theory and combinatorics are immediate...

Ngày tải lên: 08/08/2014, 12:22

Ngày tải lên: 11/08/2014, 11:21

Ngày tải lên: 12/08/2014, 03:21

Ngày tải lên: 12/08/2014, 05:20

Ngày tải lên: 05/09/2013, 16:10

Ngày tải lên: 07/09/2013, 13:48

Ngày tải lên: 07/09/2013, 13:48

... and retu o appear in the string following the number. use parseInt( ) and parseFloat( ) ber at the beginning n-numbers. parseInt( ) parse rs and floating-point numbers. If ) interprets it as ... behavior inherited from Perl: if the specified separator character is a single space, it discards any leading and trailing whitespace in the string before splitting the remainder of the string. being ... "NaN" As is false Number object Infinity "Infinity" As is true Number object Negative infinity "-Infinity" As is true Number object Any other String value of true Number...

Ngày tải lên: 05/10/2013, 12:20