... ⁄ 04297-8, 01 ⁄ 07544-9 and 04 ⁄ 04933-2), CNPq and CAPES, and NIH NS39892 and RR19325 (to MJG) PAGG and CCR were supported by studentships from FAPESP (02 ⁄ 09861-4 and 97 ⁄ 05500-7, respectively) ... Duoscan T1200 scanner and final composites were prepared using Adobe’s photoshop without modifying the original data Site-direct mutagenesis and protein expression Double-stranded site-directed ... performed independently and exhibiting similar results a hypothetical intermolecular interaction using calcium as a ligand was performed (Fig 7) The calcium is modeled in green and is hydrogen bonded
Ngày tải lên: 30/03/2014, 16:20
... antimicrobial peptide candidates for both human and nonhuman therapeutants from genomic sequences and will aid in understanding the evolution and transcriptional regulation of expression of these ... PCR-amplified bands corresponding to pleurocidins WF1, WF2, WF3 and WF4 [5]. Positively hybridizing clones were picked and replated until 100% purity was achieved and mapped using BamHI, SstI, XhoI and ... brain, muscle and skin (20–50 mg tissue) of an adult winter flounder, and from pooled samples of 20 whole larvae (hatch and 9 d.p.h.), 10 whole larvae (15, 20, 25 30 and 36 d.p.h.) and five whole
Ngày tải lên: 31/03/2014, 07:20
structure and written
... having Example Part B andles have been use since the prehistoric era A B C D The topics of the sentences in the Structure and Written Expression Section are usually academic, and many of them express ... facts What the Structure and Written Expression Section is designed to test Very good question, well, concentrate on noun, verb, adjective, prepositional and adverbial phrases, and subordinate ... section, the Structure and Written Expression section the Reading Comprehension section ♥ In addition to good listening skills, you need to have solid skills in grammar, vocabulary, and reading
Ngày tải lên: 04/12/2016, 12:21
Báo cáo khoa học: Gain of structure and IgE epitopes by eukaryotic expression of the major Timothy grass pollen allergen, Phl p 1 pdf
... comprising Zea m and Cyn d 1, a second consisting of Lol p 3, Dag g 3, Lol p 1, Phl p 1, Ory s and Tri a and a third cluster including Lol p 2, Hol l 1, Phl p and Pha a Although Phl p and Phl p are ... amplification Expression and purification of rPhl p from baculovirus-infected insect cells The expression of rPhl p in insect cells was optimized by infecting Sf9 cells with different amounts of virus and ... virus and by expression for various periods Aliquots of the culture supernatants and cell pellets were analyzed by SDS ⁄ PAGE and immunoblotting with a rabbit anti-Phl p antiserum and a monoclonal
Ngày tải lên: 16/03/2014, 18:20
Tài liệu Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB pdf
... [8] and for i ts absence i n T. pallidum and Borrelia [9,10]. Limited structural analysis suggests that several o ral treponemes (T. brennaborense and T. maltophilium [6], T. medium [11], and ... allow u s to p ropose the structures of oligosaccharides 1 and 2, as presented in Scheme 1. The o ligosaccharides were further analyzed by CE-MS and by CE-MS/MS (Figs 5 and 6). The mass spectra obtained ... out on Sephadex G-50 (2.5 · 95 cm) and Sephadex G -15 columns (1.6 · 80 cm) in pyridinium-acetate buffer, pH 4.5 (4 mL of pyridine and 10 mL of AcOH in 1 L of water), and the eluate was monitored by
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf
... attributed to the helical structure. The dottedandsolidtracesareexperimentalanddeconvoluteddata, respectively. Table 2. S econdary structure and helix orientation of IFP–wt and -F13 in DMPC/DMPG ... the membrane bilayer obliquely and possess substantial regular structures (a-helix and b-sheet) in t he membranous medium, in supp ort of the idea that balanced helix and b-sh eet structures are necessary ... IFP–wt and analogues for self-assembly in the membranous medium. Helical structure and insertion angle of the membrane-associated fusion peptide as measured by FT-IR spectroscopy The secondary structure
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf
... activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L 2 and Chlamydophila psittaci 6BC Holger Heine, Sven Mu¨ ller-Loennies, Lore Brade, Buko Lindner and ... C18:0, C20:0 and 3-OH-C20:0. MALDI-MS and ESI-MS analysis As a first step in the structural analysis, LPS and de-O- acylated LPS of C. trachomatis serotype E was analyzed by MALDI-TOF and ESI-FT-ICR ... (TSBP) which lacks the terminal Kdo, and tetrasaccharide 1-monophosphate (TS 1-MP). One-dimensional 1 H- and 31 P-, and two-dimen- sional homo- ( 1 H, 1 H-DQF-COSY) and heteronuclear ( 1 H, 13 C-, 1 H, 31 P-HMQC)
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... T and TG (T ⁄ TG), N and G (N ⁄ G), G and TG (G ⁄ TG), and N and T (N ⁄ T) structures are superimposed Effects of (A) L-glutamate (T ⁄ TG and N ⁄ G) and (B) Tris (G ⁄ TG and N ⁄ T) on the structure ... phasing model for the N structure The G and TG structures were solved using the N structure as a search model, and the TG structure was used in phasing of the T structure The structures were refined ... backbone atoms of Mglu and Bacillus glutaminase induced by ligand binding Displacement values for backbone atoms of the nonliganded and liganded structures are shown The average and SD of these conserved
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... three antiparallel strands (1, and 7) and strand 6, which is parallel to strand b-Strands are located between the four a-helices (a1, 278–294; a2, 305–313; a4, 374–381; and a5, 397–405), with ... elements: b-strands (b3, 329–335; b4, 339–344; and b5, 367–372) and a distorted a-helix (a3, 348–356) (Fig 3B,C) The three b-strands of the minidomain are all antiparallel, and form a single b -structure ... the structure of the protein core (residues 277–328 and 373–413) in solution and in the solid state, and indicates their similarity A superposition of the C-domain NMR structure on the crystal structure
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... we present here the apo structure of S. aureus EF-G. Overall structure and comparison with previous EF-G structures All five domains of S. aureus EF-G are ordered in our structure. The overall ... the A B C Fig. 1. EF-G structure. (A) Overall structure and structural domains of S. aureus EF-G (PDB 2xex). The switch regions are shown in black, with switch II facing domains II and III, and switch I ... helices A V and B V at the surface of domain V. Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA. The two helices are facing the ribosome, and the four-stranded
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... three-dimensional structure, and catalytic mechanism [5,6] Family 18 and family 19 glycoside hydrolases both contain chitinases Members of the two families have very different three-dimensional structures and ... with respect to its catalytic properties and crystal structure The crystal structures of ChiG and ChiC [18] permitted structural comparison between bacterial and plant enzymes, which provided a structural ... extended towards Cys183 in ChiC (and in the plant enzymes) Compared to the barley enzyme, ChiC and ChiG lack three loops (A, B and C) and a C-terminal extension (Figs and 4B) In addition, one other
Ngày tải lên: 07/03/2014, 11:20
THE MINE WATER POLLUTION THREAT TO WATER RESOURCES AND ITS REMEDIATION IN PRACTICE pdf
... RESOURCES AND ITS REMEDIATION IN PRACTICE PAUL L. YOUNGER Pollution by mine drainage is a major problem in many parts of the world. The most frequent contaminants are Fe, Mn, Al and SO 4 , ... Zn, Cu, As, Ni, Cd and Pb. Remedial options for such polluted drainage include monitored natural attenuation, physical intervention to minimise pollutant release, and active and passive water ... application of this decision-making process in practice: Wheal Jane and South Crofty (Cornwall), Quaking Houses (Co Durham), Hlobane Colliery (South Africa) and Milluni Tin Mine (Bolivia). In many
Ngày tải lên: 15/03/2014, 16:20
Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... black and grey bars represent the 0.9 and 1.5 kb tran- scripts, respectively. Error bars denote the standard error of the mean for three independent measurements. Genomic structure and expression ... of these have been purified and characterized as alkaline [12] and acidic RNases [13], and a third poly(U)- and poly(C)-specific ribo- nuclease has also been isolated and characterized [14,15]. ... signals are boxed with a white background, and the poly(T) tracts and ATTTA motifs in the 3¢ UTR are boxed with a gray background. Genomic structure and expression analysis of the Cc RNase T. N.
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
... Hemoglobin and Myoglobin and Their Reactions with Ligands Elsevier, North-Holland Publishing, Amsterdam, the Netherlands Dickerson, R.E & Geis, I (1983) Hemoglobin: Structure, Function, Evolution and ... the crystal structure of Pe metHbH2O [16] and as confirmed herein by solution 1H NMR of Pe metMbCN Proximal and distal residues are shown as rectangles and circles, respectively, and arrows connecting ... ligand to participate in H-bonding to the ligated cyanide The van der Waals surfaces for the Tyr32(B10) Og and the 10° tilted cyanide ligand are shown in Fig and establish that the Tyr Og and
Ngày tải lên: 23/03/2014, 17:22
Storage Structure and Relationships pdf
... size [...]... database can be created with a standard block size and up to four nonstandard block sizes • Block sizes can have any power-of-two value between 2 KB and 32 KB 9-12 Copyright © Oracle Corporation, ... utilization • Obtain storage structure information 9-32 Copyright © Oracle Corporation, 2002 All rights reserved Practice 9 Overview This practice covers identifying and obtaining information ... following: • Describe the logical structure of the database • List the segment types and their uses • List the keywords that control block space usage • Obtain storage structure information 9-3 Copyright
Ngày tải lên: 29/03/2014, 16:20
Báo cáo khoa học: Functional aspects of the solution structure and dynamics of PAF – a highly-stable antifungal protein from Penicillium chrysogenum pdf
... Asn12 and Lys17 (region 2) and between Lys34 and Asp39 (PAF) and Lys31 and Asp36 (AFP) (region 3). Two conserved GlyLys motifs are repeated in the structure, the glycines (i.e. Gly5 from b1 and ... adjacent Lys9 and Lys39. Similarly, Asp23 stabilizes the juxtaposed Lys15 and Lys17 side chains. The hydrophobic strand b4 is located between Cys43 and Asp46 and, as a central strand of sheet ... [14]. The 3D molecular structure of PAF consists of five b-strands connected by three small loops involving b-turn motifs (loops 1, 2 and 4) and the large loop 3 (Fig. 9). The b-strands create two orthogonally
Ngày tải lên: 29/03/2014, 23:20
Báo cáo khoa học: Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide pdf
... Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide Katarina Stark*, Miroslav Dostalek and F. P. Guengerich Department of Biochemistry and Center in ... epoxides (EETs) of ananda- mide [11]. Administration of anandamide to rats increased the levels of P450 in the 2C and 3A subfami- lies in rat liver and brain [30]. The in vivo formation and biological ... dog, rat, mouse and chimpanzee orthologs, respec- tively. Kidney, breast and aorta all expressed P450 4X1 mRNA at 5- and 10-fold higher levels than adult liver, and in prostate the expression was
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf
... and characterized, the stereochemistry of the reaction analyzed, and the gene encoding it cloned and sequenced [3–5] Furthermore, its crystal structure was recently determined [6] Molecular and ... competitive 0.92 and 518 versus noncompetitive 0.95 and 351; isocitrate, competitive 0.98 and 121 versus noncompetitive 0.99 and 58 Site-directed mutagenesis of ArCMLE1 From the crystal structure of ... EFGH and IJKL tetramers, and residues 89–104, which form a helix–loop structure on the surface Deviations between monomers of the two structures are in the same range as within independent structures
Ngày tải lên: 30/03/2014, 10:20
Aesthetic and Ethical Implications of Participatory Hypermedia Practice pdf
... non-being, into which you are thrust and to stand up demanding to be seen and heard to make yourself visible, to claim that your experience is just as real and normative as any other. (Invisible ... an understanding of real-time participatory hypermedia construction practice, but it is central to a full understanding of the role and its context Narrative... situations and point ... hypermedia authoring, and a grounded theory and critical incident analysis of in situ expert practice. I have constructed a preliminary taxonomy of practitioner “moves” and performed a deep analysis
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot
... 12% (d), 25% (e) and 50% (f). Ó FEBS 2004 Structure and expression of an ant neurotoxin (Eur. J. Biochem. 271) 2131 which is characteristic of a highly populated unordered random conformation ... letters and that of signal peptide is underlined. The final DNA products were digested with BamHI and EcoRI and inserted into the vector pFastBac, yielding, respectively, pFastBacSPx and pFastBacPx. ... of 2 H-enriched-TFE, H 2 O and 2 H 2 O was adjusted to pH 5.5. The standard COSY, TOCSY as well as 100 and 200 ms mixing time NOESY spectra were accumulated, processed by NMRPIPE [21] and analyzed by X - EASY program
Ngày tải lên: 30/03/2014, 13:20
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