tissue engineering of the liver
Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 1
... Preface This thesis is submitted for the degree of Doctor of Philosophy in the Graduate Program in Bioengineering at the National University of Singapore under the supervision of Professor Lee ... responses The development of microthin polycaprolactone (PCL) films raised the possibility of using these films as scaffolds to complement the cell sheet approach The aim of the present thesis was ... Secondly, previous successes in the engineering of large diameter blood vessels demonstrate the feasibility of vascular tissue engineering, although the engineering of small vessels are faced by...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 2
... Chapter Isolation and Characterisation Of Vascular Progenitors Figure 4-3: Primary culture of haemopoietic tissue in EGM Following culture in endothelial cell media, outgrowth colonies were ... cultured in the same medium, fibroblastic cells quickly grew out from fetal liver mononuclear cells, and failed to express endothelial phenotype 122 Chapter Isolation and Characterisation Of Vascular ... Progenitors (a) (b) (c) (d) (e) (f) Figure 4-4: CFU assay Adherent colonies appeared after ten days of culture, and were stained with crystal violet to facilitate visualization Cobblestone colonies...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 3
... resulted in the generation of a haemangioblast-like population However, these cells were incapable of further expansion In the light of these findings, UCB currently represent the best source of EPC ... previously reported 16% of fetal liver cells expressing CD133 and 8% expressing VEGR2 during the 18th week of gestation (Nava 2005), further suggesting the presence of EPC in the liver The discrepancies ... suggest the presence of EPC in the fetal liver, I then proceeded to study the possible selection of EPC using immunoselection CD133, a stem/progenitor cell marker, was chosen because of the reported...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 4
... and then the introduction of an N1speak following the conjugation of the CD34 antibody, indicating presence of the protein High resolution surveys of the C1s core spectra revealed the presence of ... conjugation of biomolecules These developments contribute towards improved biocompatibility of the tissue engineering scaffolds In the specific context of vascular tissue, two other important classes of ... analysis of the fetal liver cells suggests the presence of EPC Thus, I proceeded to use MACS enrichment of CD133 expressing cells from the fetal liver The CD133 enriched population was reminiscent of...
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HYDATID DISEASE OF THE LIVER IN CHILDREN ppt
... in the Journal, the copyright of the paper shall become the property of The Liverpool School of Tropical Medicine who may on request licence me/us to reproduce the paper of any part thereof, ... DISEASE OF THE LIVER IN CHILDREN ABSTRACT: Twenty one patients with hydatid disease of the liver were operated upon between 1986-1997 There were 10 males and 11 females The mean age of the patients ... drainage of the residual cavity was carried out One patient had introflexion of the cyst cavity (obliteration of the cavity by folding the cyst wall inside the cavity and suturing the outer surface...
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Báo cáo khoa học: Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger docx
... and in the range of the CV of the NADPH determination Discussion Obvious tendencies found by characterization of the wild-type In the exponential phase of the ammonium cultures all of the PPP ... [26] Overproduction of 6PGDH resulted in an increase of synthesis of pyridine nucleotide cofactors, as the total pool of these increased Whether the synthesis is regulated by the NADPH concentration ... Figure 4B shows the X-loading weights of the X-variables (measured variables other than NADPH) and the Y-loading of NADPH on the two PCs As NADPH is in the first quadrant of Fig 4B (the four quadrants...
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Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens potx
... concordance between the presence of the mRNA and of the A antigen in tissues Nevertheless, there were some discrepancies in tissues such as the kidney and the urinary bladder where the mRNA was easily ... that one of the two other known members of the family, namely the iGb3 synthetase and the Forssman synthetase, could have a dual specificity Alternatively, there could exist another gene in the rat ... the parietal cells of the gastric glands whereas the B antigen was present on the pits epithelial cells and the chief cells of the glands In the large intestine and the caecum, the A antigen was...
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Xơ gan (cirrhosis of the liver) docx
... Giảm chức gan Phân loại xơ gan Theo nguyên nhân bệnh sinh: Xơ gan cửa (vòng, Laennec, xơ gan Banti) Xơ gan rượu Xơ gan sau hoại tử Xơ gan sau viêm gan Xơ gan mật Theo hình thái: (kích thước hạt...
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Ung thư gan ( carcinoma of the liver) doc
... thấy chất nhày Di ung thư gan: - Ung thư gan thường di muộn đến hạch, tạng ổ bụng gần gan - Di theo đường máu thường đến phổi - Thời gian sống thêm năm sau chẩn đoán 10% Ung thư gan thể lan tràn ... trúc hình xà cừ; giúp phân biệt với u mạch máu, nốt tăng sinh, di gan - Chụp mạch máu: luồn catheter qua ĐM đùi vào ĐM gan, phát u cấu trúc giàu mạch máu tân sinh Có thể đồng thời làm tắc TM ... 3) XN phát di xa: X quang phổi, xạ hình xương, CT sọ não VI PHÂN LOẠI TNM - ĐÁNH GIÁ GIAI ĐOẠN THEO AJCC: o Khối u: - T1 : u đơn độc không xâm lấn mạch máu - T2: u đơn độc xâm lấn mạch máu hay...
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Báo cáo khoa học: " Imaging evaluation of the liver using multi-detector row computed tomography in micropigs as potential living liver donors" pdf
... human liver, in this study, our data suggest that the total liver volume of micropigs might be sufficient to support the functions of the human liver in terms of the liver volume needed for liver ... compare the data between micropigs and human, the total Imaging evaluation of the liver in micropigs 95 volume and the right lobe volume of the liver parenchyma were evaluated, because usally the ... significant Results The CT images of the liver parenchyma are illustrated in Fig 1, and the total liver and right lobe volumes were calculated (Table 1) The liver parenchyma of the micropigs showed...
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Báo cáo y học: "Tissue engineering in the rheumatic diseases" ppsx
... microfracture resulted in the migration of ovine MSCs into the implant and in the formation of cartilage-like repair tissue Controls treated with microfracture showed no formation of repair tissue ... spatial distribution of chondrocytes and the lack of initial mechanical stability, (b) the suture of the periosteal flap into the surrounding healthy cartilage and the necessity of a perifocal solid ... sources, the open question is whether their possible advantages justify the labor and cost-intensive launch of a new clinical product Tissue engineering and advanced threedimensional models Tissue engineering...
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báo cáo khoa học: "Inflammatory pseudo-tumor of the liver: a rare pathological entity" potx
... ulcerations of the epithelial cell layer Then the ongoing efflux of toxic bile into the portal field; together with activation of biliary epithelial cells, leads to proliferation and activation of periductal ... of the liver: clinicopathologic study and review of the literature Cancer 1990, 65:1583-1590 Shek T, Ng I, Chan K: Inflammatory pseudotumour of the liver: report of four cases and review of the ... proposed for the possible etiology of inflammatory pseudotumour of the liver We think that the significant inflammatory effect of the biliary steroid lithocholic acid is a major contributor in the formation...
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báo cáo khoa học: "Solitary fibrous tumor of the liver: a case report" docx
... Thus, the radiological findings are nonspecific and cannot distinguish between benign and malignant tumors Therefore, in the present case, the diagnosis of SFT of the liver was based on the association ... of SFT of the liver Correct interpretation of unique pathological findings and CD34 immunoreactivity plays a significant role in differentiating SFT from other spindle-cell neoplasms of the liver ... review of the literature revealed fewer than 40 reported SFTs of the liver to date [7,8,10] These tumors mainly occurred in women, at a ratio of 71.7 percent female to 28.3 percent male The mean...
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Báo cáo khoa học: "Primary undifferentiated embryonal sarcoma of the liver mistaken for hydatid disease" pptx
... contributions WF drafted the manuscript, NEM and DM participated in the design of the study, AcS assisted with the collection of data and conceived of the study, MK and AlS participated in the design and ... sarcoma of the liver in childhood resembling hydatid cyst of the liver Br J Radiol 1997, 70:314-316 Zaheer W, Allen SL, Ali SZ, Kahn E, Teichberg S: Primary multicystic embryonal sarcoma of the liver ... three reports of liver transplantation for UES in children [13-15] however the use of liver transplantation for primary hepatic sarcoma in adults is controversial There are no reports of liver transplantation...
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Báo cáo khoa học: "Highly malignant soft tissue sarcoma of the extremity with a delayed diagnosis" ppsx
... sarcoma were diagnosed Four of the seven cases of synovial sarcoma, two of the three cases of alveolar soft part sarcoma, two cases of myxofibrosarcoma, and one case each of high-grade leiomyosarcoma ... reasons, six of the seven were excised without sufficient biopsy or examination The characteristics of the alveolar soft portions of sarcomas are that they comprise only 0.5-1.0% of soft tissue sarcomas, ... study, one of the three cases of alveolar soft part sarcoma occurred in adolescence, and the other two occurred in young adults In two of these three cases, the chief complaint was of a painless...
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Báo cáo khoa học: "Primary carcinoid tumors of the liver" ppsx
... heterogenous depicting a ofof the left lobe of the liver solid mass in the Ultrasound 6.3 the abdomen; Ultrasound of the abdomen latUltrasound of the abdomen; Ultrasound of the abdomen depicting ... heterogenous solid mass in the lateral segment of the left lobe of the liver Microscopically, the tumor consisted predominantly of solid sheets and organoid nests of uniform, intermediatesized, ... other metastatic sites included a negative CT scan of the chest, upper and lower gastrointestinal endoscopy, and a Technetium-99m Figure of enhancing, the IV marginated mass CT scan of the the...
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Báo cáo khoa học: " Neoadjuvant chemoradiation compared to neoadjuvant radiation alone and surgery alone for Stage II and III soft tissue sarcoma of the extremitie" pps
... B: Phase II study of neoadjuvant chemotherapy and radiation therapy in the management of high-risk, high-grade, soft tissue sarcomas of the extremities and body wall: Radiation Therapy Oncology ... Merino MJ, Rosenberg SA: Randomized prospective study of the benefit of adjuvant radiation therapy in the treatment of soft tissue sarcomas of the extremity J Clin Oncol 1998, 16:197-203 O’Sullivan ... R: The treatment of soft -tissue sarcomas of the extremities: prospective randomized evaluations of (1) limb-sparing surgery plus radiation therapy compared with amputation and (2) the role of...
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Diseases of the Liver and Biliary System - part 1 potx
... to the skin into the liver substance and extracted This is the quick part of the procedure The tip of the needle is now placed on sterile paper and some of the remaining saline flushed through the ... 645 Other causes of extrinsic pressure on the common bile duct, 645 37 Tumours of the Gallbladder and Bile Ducts, 647 Benign lesions of the gallbladder, 647 Carcinoma of the gallbladder, 647 Other ... depends on the acinar location of damage [28] The liver cells (hepatocytes) comprise about 60% of the liver They are polygonal and approximately 30 mm in diameter The nucleus is single or, less often,...
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Diseases of the Liver and Biliary System - part 2 pot
... lesion in the left lobe of the liver (b) Following enhancement, dynamic scanning shows gradual infilling of the lesion which eventually became isodense with the remainder of the liver These are the ... palms) The hands are warm and the palms bright red in colour, especially the hypothenar and thenar eminences and pulps of the fingers (fig 6.12) Islets of erythema may be found at the bases of the ... describes the rate at which the axes of the protons move out of phase with each other because of the differing electromagnetic influence of adjacent protons Protein density simply depicts the number of...
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Diseases of the Liver and Biliary System - part 3 potx
... veins of the caval system Deviation of blood into these channels leads to varicosities in the submucous layer of the lower end of the oesophagus and fundus of the stomach (b) At the anus, the superior ... by the union of the superior mesenteric vein and the splenic vein just posterior to the head of the pancreas at about the level of the second lumbar vertebra It extends slightly to the right of ... attempting to bypass the block and return blood towards the liver These enter the portal vein in the porta hepatis beyond the block They include the veins at the hilum, venae comitantes of the portal vein...
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