timing of protein intake with exercise

... constancy of output of creatinine during the experiment, confirming in general the modern idea of the specificity of this metabolism ARTICLE: THE INFLUENCE OF PROTEIN INTAKE UPON THE FORMATION OF URIC ... output of uric acid merely as the expression of overwork, the result of the excessive activity of grandular cells in the digestion, assimilation and catabolism of the unusual input of protein ... experiments we had failed t’o observe any influence of moderate variations in the protein of the diet upon the uric acid output Evidently an unusual excess of protein input is required to provoke the phenomenon...

Ngày tải lên: 14/07/2015, 09:44

... variants of the human POLDIP3 gene encoding proteins of 421 amino acids with a molecular mass of 46 kDa and 392 amino acids with a molecular mass of 43 kDa, which differ only by an insertion of 29 ... secondary structure of ER which suggests that CK2 may regulate the activity of ER [2] The Xenopus ER was identified as one of the proteins interacting with dimerization cofactor of hepatocyte nuclear ... a band of a protein with electrophoretic mobility of approximately 46 kDa, which corresponds to the molecular mass of isoform ⁄ a of PDIP46 ⁄ SKAR (Fig 3C) The band of this protein was excised...

Ngày tải lên: 19/02/2014, 05:20

... cases of false predictions with high values of reliability index, by comparing with the presently available data base of interacting complexes the accuracy may be biased by the lack of knowledge of ... structure of some of the complexes or by other experimental means CONCLUSIONS We have analysed the possibility of predicting the residues forming part of protein protein interacting surfaces in proteins ... Proteins 41, 47–57 16 Valdar, W.S.J & Thornton, J.M (2001) Protein protein interfaces: analysis of amino acid conservation in homodimers Proteins 42, 108–124 Ó FEBS 2002 Predicting protein protein...

Ngày tải lên: 21/02/2014, 15:20

... interaction of HIP1 with normal Htt (a protein with fewer than 36 Gln) is stronger than that observed with the mutated Htt (a protein with more than 36 Gln residues) [10] On the basis of this observation, ... assay of pDED of HIPPI with the caspase-1 gene upstream sequences In panel I, typical results of EMSA with the 717 bp caspase-1 probe and 6X(HN)-pDED of Hippi and 6X(HN)-tagged N-terminal domain of ... concentrations of NaCl, the fluor3888 escence intensities of GST–HIPPI increased, indicating a lesser extent of interactions of GST–HIPPI with AA AGACATG This result indicated that the interaction of GST–HIPPI...

Ngày tải lên: 07/03/2014, 10:20

... analogs was confirmed by MALDI-TOF ⁄ TOF MS, with a 4800 MALDITOF ⁄ TOF Analyzer (AB SCIEX, Framingham, MA, USA) MALDI-TOF and TOF ⁄ TOF MS spectra were acquired with 800–1000 and 2000–2800 laser shots ... crossreacts with bovine PDI [27], was a generous gift from M Hubbard (University of Melbourne) Purification of bacitracin analogs and MALDI-TOF ⁄ TOF MS analysis The nomenclature of Ikai et al ... bond with free PDI cysteines Fig Disulfide bond formation between bacitracin and Cys345 on PDI (A) Scheme of thiol formation of the thiazoline ring of bacitracin A with subsequent formation of a...

Ngày tải lên: 14/03/2014, 23:20

... natural deconvolution of circular dichroism curves of proteins Protein Eng 4, 669–679 ´ Dousseau, F & Pezolet, M (1990) Determination of the secondary structure content of proteins in aqueous solutions ... error of all analyses are determined with Ôn À 1Õ degrees of freedom in PLS-1, and Ôn À (s À 1)Õ degrees of freedom for simultaneous methods, where n is the number of spectra and s is the number of ... average value Preprocessing of protein spectra for statistical analysis CD spectra of proteins were scaled based on the protein (amino acid residue) concentration of the sample used to collect...

Ngày tải lên: 16/03/2014, 18:20

... nuclei stained with the DNA dye Hoechst; GFP, indicates the localization of the green fluorescent protein; RFP, indicates the localization of the red fluorescent protein GFP + RFP, merging of green and ... because of the high stability of mature 18S rRNAs formed prior to the depletion of Utp25p Reaction with primer P3, which hybridizes downstream of site D in ITS1, also shows the accumulation 2842 of ... co-immunoprecipitated with ProtA–Utp25p, further indicating that this protein is associated only with the early prerRNAs This is in accordance with the hypothesis of the involvement of Utp25p in the...

Ngày tải lên: 22/03/2014, 21:21

... 4B) This agrees with the presence of a larger residue that makes stable contacts with this region of DNA The interaction of mutants at position 50, and particularly of I50K–HAKN1 with DNA, provides ... N-terminal arm of MATa2 is not able to interact with DNA within the context of the HAKN1 homeodomain as it does within MATa2 Poor binding may arise from incorrect folding of the chimeric protein or ... study, we investigated the interaction of the homeodomain of the KNOX protein HAKN1 with DNA As no structural studies on the interaction of any KNOX protein with DNA have been reported, ours constitutes...

Ngày tải lên: 23/03/2014, 13:20

... Detyrosination of microtubules by nonassociated carboxypeptidase in intact cells COS cells were treated with or without lM OA for h and subsequently with or without 10 lM taxol Time of taxol addition ... showed no increase of Glu-tubulin, and cells treated with taxol alone (+taxol) were detyrosinated faster than cells treated with OA and then with taxol Evaluation of the slope of the curves indicated ... Cleavage of structural proteins during the assembly of the head of bacteriophage T Nature (London) 227, 680–685 20 Towbin, H., Staehelin, T & Gordon, J (1979) Electrophoretic transfer of proteins...

Ngày tải lên: 23/03/2014, 15:21

... enhancement of the activity of endogenous E3s or the inhibition of proteasomal degradation of polyubiquitinated proteins Colocalization of RBCK2 with the 26S proteasome The subcellular localization of ... NESs and A B 6376 Fig Effect of overexpression of RBCK2 on accumulation of polyubiquitinated proteins (A) Schematic drawing of RBCK1 and RBCK2 Amino acid residue numbers of the N-termini and C-termini ... activities [9] RBCC protein interacting with protein kinase C2 (RBCK2), a splice variant of RBCK1 (Fig 1A), consists of 260 amino acids and shares the N-terminal 240 amino acids with RBCK1 RBCK2...

Ngày tải lên: 29/03/2014, 22:21

... discuss the role of a V-shaped structure of FKBP22 Results Design of monomeric mutant protein The monomeric mutant protein (NNC-FKBP22) was designed based on a model of the 3D structure of WT (SIB1 ... to that of WT without one arm of the ‘V’ A model of the 3D structure of NNC-FKBP22 is shown in Fig 1A Its primary structure is also schematically shown in Fig 1B in comparison with that of WT NNC-FKBP22 ... tertiary models of SIB1 FKBP22 and NNC-FKBP22 Numbers indicate the positions of the residues relative to the initiator methionine residue of the proteins without a His-tag The ranges of the N- and...

Ngày tải lên: 30/03/2014, 01:20

... which regions of the protein make contact with the ligand, we carried out a series of Structure and interactions of SlyD A turn B C Fig Structural alignment between the PPIase domains of SlyD and ... analysis of the changes in the far-UV CD spectra of the protein upon titration with nickel ions (Fig 6) Dramatic changes in the molar ellipticity of SlyD were in fact observed up to a : nickel ion protein ... process of target peptides, consistent with what has been suggested by Weininger et al [31] As expected by comparison with other FKBP proteins, key catalytic residues within the PPIase domain of SlyD...

Ngày tải lên: 30/03/2014, 01:20

... amounts of proteins were incubated overnight at °C with lg of antibodies to STAT5B and to c-Jun (Santa Cruz Biotechnologies, Santa Cruz, CA, USA) Immune complexes were precipitated with 20 lL of protein ... expression of the AGT gene in VSMCs stimulated with Ang II, the effect of Ang II on the phosphorylation of STAT5 and c-Jun was measured Ang II stimulated the phosphorylation of STAT5B and c-Jun, with ... complemented our in vitro data with the studies on protein protein and protein DNA interactions and demonstrated that the requirement for the interaction of AP-1 with STATs for the AGT promoter...

Ngày tải lên: 30/03/2014, 02:20

... Characterization of bHLH-PAS-A of NPAS2 Y Mukaiyama et al Table Resonance Raman spectra of Fe(II)–CO complexes of the basic helix–loop–helix (bHLH)-PAS-A and PAS-A domains of neuronal PAS domain protein ... consisting of 100 mM Tris (pH 7.5) All proteins were His-tag-free Table Optical absorption spectra of isolated basic helix–loop– helix (bHLH)-PAS-A and PAS-A domains of neuronal PAS domain protein ... examine the nature of the heme environment of the bHLH-PAS-A domain of NPAS2, we analyzed the resonance Raman spectra of the Fe(III), Fe(II) and Fe(II)–CO complexes The spectra of the Fe(III) and...

Ngày tải lên: 30/03/2014, 11:20

... (1993) Molecular mechanism of inhibition of mammalian protein synthesis by some four-chain agglutinins: proposal of an extended classification of plant ribosome-inactivating proteins (rRNA N-glycosidases) ... comparison with the present structure Weak diffraction is often associated with high solvent content and a large unit cell However, Krauspenhaar et al [26] recently reported the structure of MLI with ... RIPs, the number of matched Ca atoms for MLI and ricin was 469 (91.8% out of ˚ ˚ 511) with rmsd 0.92 A, and 479 (93.7%) with rmsd 1.05 A ˚ for MLI and abrin-a, using a 3.0-A cut-off distance As...

Ngày tải lên: 31/03/2014, 01:20

... were linear with time and protein Determination of NAD content The content of NAD (the sum of its reduced and oxidized forms) in Xenopus tissues was determined according to the method of Nisselbaum ... inhibition of [125I]T3 binding to xCTBP/ xALDH1 was achieved with T3 and D-T3 at a concentration of 18 nM, with T4 at 450 nM and with Triac at 15 lM (Fig 1A) ALDH1 catalyzes the formation of retinoic ... at 24 °C with lg of xCTBP/xALDH1 in the presence of various concentrations of T3 The inset illustrates the Hill plot, log[vc/vi)1] vs the logarithm of T3 molar concentration, the slope of which...

Ngày tải lên: 31/03/2014, 21:21

... composed of the following: µl of first-strand cDNA, 70 µl of deionised water, 10 µl of 10 × Advantage PCR Buffer, µl of 50 × dNTP mix, µl of 5' PCR Buffer, µl of 3' PCR Buffer, 10 µl of GC-Melt ... fusion protein in the two separate yeast strains Through the analysis of sequences of positive clones, only one had a library insert that was in frame with the activation domain of GAL4 protein ... calculated cut-off of 0.179, thus indicating the presence of HBsAg The results of this assay run were valid as the criteria for the quality control had been met The mean A450 of the negative...

Ngày tải lên: 19/06/2014, 08:20

... composed of the following: µl of first-strand cDNA, 70 µl of deionised water, 10 µl of 10 × Advantage PCR Buffer, µl of 50 × dNTP mix, µl of 5' PCR Buffer, µl of 3' PCR Buffer, 10 µl of GC-Melt ... fusion protein in the two separate yeast strains Through the analysis of sequences of positive clones, only one had a library insert that was in frame with the activation domain of GAL4 protein ... calculated cut-off of 0.179, thus indicating the presence of HBsAg The results of this assay run were valid as the criteria for the quality control had been met The mean A450 of the negative...

Ngày tải lên: 20/06/2014, 04:20

... tesno eht gniwollof evren citaics eht ni desaercni yltneisnart si KNJ fo mrof detalyrohpsohp eht fo noisserpxe eht taht yduts tneserp eht ni demrifnoc ew ,deredisnoc si noitamrofni eht fo lla ... gnidrocca demrofrep ,gnilebal -dne kcin yb detceted saw noitatnemgarf AND utis ni ytivitcaeronummi001S dna 1DE ,KNJ-p dna sisotpopa fo gniniats elbuoD llec emas eht ni ezilacol-oc ot 1DE rof ydobitna ... tnatanrepus ehT yassa tolbonummi eht rof desu saw )ASU ,gnilangiS lleC ;sulPohpsohP( tik ydobitna KNJ/KPAS ehT detsevrah saw tnatanrepus eht hcihw retfa ,nim 02 rof mpr 000,21 ta degufirtnec dna sebutorcim...

Ngày tải lên: 07/08/2014, 18:21

... minute of cells cultured with peptide divided by the counts per minute of cells cultured in medium only) Statistics AA was induced by intradermal injection at the base of the tail with 0.1 ml of ... shows the possible usefulness of MMP peptide immunotherapy in arthritis, based on modulation of the T-cell response However, a clear understanding of proper timing of MMP peptide-based immunotherapy ... Miltenburg AM: Induction of tolerance with intranasal administration of human cartilage gp-39 in DBA/1 mice: amelioration of clinical, histologic, and radiologic signs of type II collagen-induced...

Ngày tải lên: 09/08/2014, 03:24