thermal modulation of radiation induced dna damage responses

... demonstrate that apoptosis induced by agarose is a component of total DNA breakage, and that the inhibition of apoptosis allows for more proper estimation of radiation- induced DNA DSB repair and apoptosis ... absence or presence of zvd-fmk (10 μM) Optical density was normalized to radiationinduced initial damage in absence of, or presence of zvd-fmk In absence of zvd-fmk, spontaneous DNA fragmentation ... hours of incubation time: 3.44% of cell population), a finding that also precludes a substantial radiationinduced origin of apoptosis and corroborates the participation of agarose in spontaneous DNA...

Ngày tải lên: 09/08/2014, 09:20

... apoptosis N Bitomsky & T G Hofmann Fig Regulation of DNA damage -induced cell death by p53 and HIPK2 Genotoxic stress -induced DNA damage facilitates activation of the DNA damage- activated protein ... by the extent of DNA damage, which in turn determines whether DNA lesions can, or cannot, be repaired As unrepaired DNA damage is characterized by constant activity of the DNA damage checkpoint ... extent of DNA damage – which determines whether or not damage is reparable – the activated DDR can trigger different cellular responses Mild DNA damage is usually handled through induction of cell...

Ngày tải lên: 16/03/2014, 00:20

... cisplatin -DNA damage involves the induction of DNA repair proteins to initiate DNA repair [10] We determined if NaAsO2 and hyperthermia modulated the expression of XPC, a platinum -DNA damage recognition ... include formation of oxidative DNA damage [26], activation of the Fas pathway [27], inhibition of DNA repair [28,29], and causation of mitotic arrest and induction of apoptosis in the mitotic cells ... enhancement of cisplatin -induced cell death The goal of this study is to determine how sodium arsenite and hyperthermia modulate mechanisms of cisplatin resistance in vivo We developed murine models of...

Ngày tải lên: 20/06/2014, 07:20

... intervals After two cures of chemotherapy, the size of the Pathological findings The histopathological evaluation of the incisional biopsy material showed a tumor growth of mesenchymal character ... chest CT image of the patient The chest CT image of the patient Ionized radiation is clearly known to trigger sarcoma formation [6] Cahan et al in 1948 defined the criteria for Page of (page number ... mediastinal invasion or lesions of extremely large sizes In our patient, the size of the lesion was 50% smaller and the findings of mediastinal invasion regressed upon two cures of neoadjuvant chemotherapy...

Ngày tải lên: 09/08/2014, 07:22

... regulated the immune responses to CII in vivo and attenuated the development of arthritis Mechanism of suppression We have reported that a major component of the mechanism of action for the synthetic ... Synovial injections The hind ankle joints of DBA/1 mice were injected intraarticularly with 10 ul of adenoviral vector × 107 pfu of adenovirus, containing the DNA for either luciferase, rCB11-A9, ... and analyzed using Metamorph software (Universal Imaging Co., Dowlington, PA, USA) Measurement of the incidence and severity of arthritis The incidence and severity of arthritis were determined...

Ngày tải lên: 12/08/2014, 14:22

... these DNA structures generates single-stranded DNA, the salient intermediate in the DNA- damage checkpoint response In fact, double-strand DNA breaks can also lead to stretches of single-stranded DNA ... Article 233 Cagney et al 233.3 of gene products are involved in DNA replication during the normal cell cycle, and the state of these proteins at the particular time of DNA damage may influence their ... and Rnr4), which is induced in response to DNA damage [18] In total, 341 such pairings These results suggest that the architecture of the transcriptional response to DNA damage is complex, if...

Ngày tải lên: 14/08/2014, 17:22

... all their support during the ‘darkest days’ of my life i TABLE OF CONTENTS Page List of Figures iv List of Tables v List of Abbreviations vi Summary of Thesis viii Chapter 1: Introduction Chapter ... of glutamate and GABA by HPLC 49 5.2 Time course of changes in glutamate output induced by SISE and SISE + post- treatment with LY367385 and diazepam 51 5.3 Effects of reverse microdialysis of ... release of glutamate and GABA from the piriform cortex 54 5.4 Time course of changes in glutamate and GABA output induced by reverse microdialysis of 5mM LY367385 in the piriform cortex of SISE...

Ngày tải lên: 26/11/2015, 10:50

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Ngày tải lên: 07/08/2014, 18:21

... Cisplatin -induced DNA damage response At sites of DNA damage, the Ser129-phosphorylated H2AX derivative, cH2AX, forms foci for the recruitment of factors involved in repair of DNA damage and ... Authors Journal compilation ª 2008 FEBS ´ C Vazquez-Martin et al Cisplatin -induced DNA damage response Cisplatin DNA damage DNA damage H2AX- P ( H2AX) Recovery H2AX H2AX- P ( H2AX) Psy4 Pph3 MMS Recovery ... roles of Pph3p–Psy4p–Psy2p in recovery from cisplatin- and MMS -induced DNA damage Pph3p–Psy4p–Psy2p forms a stable complex with cH2AX via Psy4p During recovery from cisplatin- or MMS -induced DNA damage, ...

Ngày tải lên: 30/03/2014, 04:20

... defined as the percentage of total PBLs death induced by the radiation dose minus the spontaneous cell death (control, Gy) Page of DNA damage assay Data related to initial DNA damage were obtained ... and incubated at 37°C for 24 hours The study of initial DNA damage was completed in the University of Granada (Spain) Initial radiation- induced DNA damage in PBLs was measured as previously described ... of the cell to detect and repair DNA damages DSB can be induced by a variety of DNA damaging agents, such as x-rays [20] Differences in cell survival may be related to the number of initial DNA...

Ngày tải lên: 09/08/2014, 09:20

... the initial number of DNA double-strand breaks (DSB) induced by x-rays in peripheral blood lymphocytes (PBL) and radiation- toxicity [17,18] Thus, increasing numbers of radiation induced DSB were ... account for the correct interpretation of data It has been observed that DNA DSB in residual foci and relaxation of DNA- loops may be linked to induction of radiation- induced apoptosis in lymphocytes ... susceptibility of normal cells against ionizing radiation It has been suggested that the radiation- induced damage measured on lymphocytes could be proportional to the acute damage evaluated on the skin of...

Ngày tải lên: 09/08/2014, 09:20

... consistent with the paradigm of “selective” immunomodulation of inflammatory responses by HDP and IDR-peptides (that is, suppression of excessive activation of NF-B in the presence of exogenous infectious/inflammatory ... itself induced the gene expression of IL-1RA by > 10-fold, and did not suppress the IL-1b -induced gene expression of IL-1RA (Figure 2c) Similarly, gene expression of another negative regulator of ... with the paradigm of the selective anti-inflammatory mechanism of host defense and IDR peptides [3,15,16] We explored the molecular mechanism of regulation of IL-1b -induced responses by IDR-1002...

Ngày tải lên: 12/08/2014, 17:22

... analysis patterns in the damage -induced gene set The four major expression patterns in the damage -induced gene set revealed by cluster analysis For each of the 112 damage -induced genes, the fold ... context, the type of DNA lesion, or the extent of damage, as p53-independent induction of this pathway was observed in other studies [19,20] interactions induction in response to DNA damage was abrogated ... use of RNA interference (RNAi) for targeted silencing of specific genes [5-7] The combination of global gene-expression profiling and RNAimediated silencing of key regulatory genes appears to offer...

Ngày tải lên: 14/08/2014, 14:21

... the impact of influenza infection and inflammation on DNA damage and DNA damage responses By studying chromatin phosphorylation as a measure of DNA damage, we show that the level of DNA damage increases ... respond by eliciting DNA damage responses (DDR), which include activation of cell cycle arrest, DNA repair, senescence or cell death, depending on the cell type and severity of DNA damage [11, 12] ... that DNA damage is induced in cells exposed to influenza both in vitro and in vivo, and the in vivo kinetics are consistent with dual roles for direct induction of DNA damage, as well as DNA damage...

Ngày tải lên: 09/09/2015, 08:16

... ANALYSIS OF ADAPTIVE RESPONSE TO DNA DAMAGE CHECKPOINT INDUCED ARREST YIO WEE KHENG B.Eng.(Hons.), M.Sc., NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY Acknowledgements Professor ... mechanism, the DNA damage checkpoint, inhibits cell cycle progression in response to DNA damage and causes cells to arrest in G2/M until the offending lesions are repaired A failure to repair DNA damage ... discussion of DNA damage checkpoint control and events associated with it, it is useful to begin with a brief description of the general regulatory landscape of the cell cycle in which the DNA damage...

Ngày tải lên: 14/09/2015, 08:23

... INFLUENZA INFECTION INDUCES DNA DAMAGE AND ROBUST DNA DAMAGE RESPONSES IN VIVO 66 3.1 Introduction 66 3.1.1 Investigation of DNA damage as a potential mechanism of influenza pathogenesis ... disease DNA damage response (DDR) pathway is a complex signaling network that has evolved to sense for DNA damages, activate cell signaling and repair DNA lesions DNA damage are surveyed via DNA damage ... following DNA damage DNA repair mechanisms are critical to neutralize genotoxic DNA damage, and they form an important component of DDR pathway Due to a wide diversity in DNA lesions, several DNA repair...

Ngày tải lên: 30/09/2015, 06:20

... sites of DNA damage, and its role might be important in the presence of physiological levels of DNA damage or at the sites of a specific subset of DNA lesions In addition to the phosphorylation of ... recruitment of checkpoint sensors to specific types of DNA damage Both DNA damage and the activation of checkpoint can lead to changes in the chromatin structure at the sites of DNA damage (see ... Although ssDNA plays a crucial role in the recruitment of ATR-ATRIP, ssDNA alone is not sufficient to elicit the checkpoint responses, suggesting that additional DNA structures induced by DNA damage...

Ngày tải lên: 25/10/2013, 21:20

... multitude of functions in the DNA damage response, one of which is to promote high fidelity repair of damaged DNA Regulation of these functions is complex and involves a variety of mechanisms, one of ... with the DNA damage pathways [53,54] Blockade of EGFR signaling results in alteration of the DNA damage response [53–56] In particular, erlotinib has been shown to decrease irradiation -induced ... irradiation and persists 50 h after irradiation This redistribution of BRCA1 follows a dose-dependent manner and utilizes a CRM1-dependent mechanism Additionally, as irradiation -induced DNA damage...

Ngày tải lên: 06/03/2014, 22:21

... thus appears to be important for DNA damage responses mediated by ATM, ATR and DNA- PKcs Role of Nbs1 in DNA damage checkpoint control The similar clinical phenotypes of AT, NBS and ATLD suggested ... of nuclear foci that form rapidly after exposure of cells to ionizing radiation (IR) and which represent sites of ongoing sensing or repair of DNA double-strand breaks Hypomorphic mutations of ... to the contribution of Nbs1 to the G1 or G2 ⁄ M checkpoints Dual role of Nbs1 in ATM-dependent DNA damage signaling The rapid localization of the MRN complex to the region of DNA strand breaks,...

Ngày tải lên: 16/03/2014, 13:20

... difference spectra of DNA photolyase and DNA (A) Oligo-(dT)18 DNA photodamage with UV radiation in the absence of photolyase (B) Photoreactivation of DNA photolyase followed by DNA photorepair (after ... environmental change of carbonyl groups during photoreduction is excluded Photoactivation of DNA photolyase Photodamage of DNA Red-light irradiation selectively induced the one-electron reduction of the blue ... an elucidation of a detailed molecular picture of the light-driven DNA repair process Results Construction of a DNA photolyase E109A mutant MTHF, the second cofactor of E coli DNA photolyase,...

Ngày tải lên: 16/03/2014, 18:20