therapeutic effects of nitric oxide synthase inhibition in neuronal injury

... and inducible (iNOS) isoforms of nitric oxide synthase (NOS) in the ovaries of pigs The intensity of staining is indicated by (-), where staining was absent, up to (+++), for maximal staining ... localization of inducible and endothelial nitric oxide synthase in the rat ovary Effects of gonadotropin stimulation in vivo J Clin Invest 1995, 96, 2719-2726 15 Xie QW, Nathan C The high-output nitric oxide ... The involvement of nitric oxide in the ovulatory process in the rat Endocrinology 1994, 135, 2287-2290 11 Takesue K, Tabata S, Sato F, Hattori MA Expression of nitric oxide synthase- 3 in porcine...

Ngày tải lên: 07/08/2014, 18:21

... Nitric oxide mediates interleukin-1 induced inhibition of glycosaminoglycan synthesis in rat articular cartilage Mediators Inflamm 1995, 4:107-111 48 Murrell GAC, Jang D, Williams RJ: Nitric oxide ... effectiveness in reducing the progression of experimental OA in the ACL dog model [53] The same study also demonstrated that iNOS inhibition reduced the synovial inflammation, a finding that is well in ... JC, Manning PT, Connor JR, Currie MG, Martel-Pelletier J: Selective inhibition of inducible nitric oxide synthase reduces progression of experimental osteoarthritis in vivo: possible link with...

Ngày tải lên: 09/08/2014, 14:20

... pharmacological inhibition of iNOS on edema in the lung Effect of2 Effect of genetic or pharmacological inhibition of iNOS on edema in the lung The injection of bleomycin in iNOSWT mice elicited an inflammatory ... administration using iNOSKO mice and iNOSWT mice In addition, we have investigated the effects of the systemic administration of GW274150 in iNOSWT mice subjected to bleomycin-induced lung injury In particular, ... by inflammation [26] Induction of lung injury by bleomycin Mice received a single intratracheal instillation of saline (0.9%) or saline containing bleomycin sulphate (1 mg/kg body weight) in...

Ngày tải lên: 12/08/2014, 18:21

... factors binding to it in LPS-induced gene expression Although involvement of octamer-binding factor-1 (Oct-1), another octamer-binding protein, cannot be excluded in the LPSinduced expression of iNOS ... Regulation of the expression of inducible nitric oxide synthase Biol Chem 384, 1343–1364 10 Kleinert H, Pautz A, Linker K & Schwarz PM (2004) Regulation of the expression of inducible nitric oxide synthase ... Src-related kinases, mitogen-activated protein kinases and phosphoinositide 3-kinase (PI3K), downstream of the TLR4-signaling pathway [3–5] Of the LPS-induced genes, those coding for inducible nitric oxide...

Ngày tải lên: 22/03/2014, 17:20

... inducible nitric oxide synthase (iNOS) by calpain I Biochim Biophys Acta 1568, 216– 224 23 Su Y & Block ER (2000) Role of calpain in hypoxic inhibition of nitric oxide synthase activity in pulmonary ... right amount of nitric oxide Am J Physiol Renal Physiol 285, 178–190 In vivo degradation of NOS and HSP90 by calpain Kone BC (2000) Protein–protein interactions controlling nitric oxide synthases ... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no...

Ngày tải lên: 30/03/2014, 04:20

... indicate differential effects of CaM binding on full-length wild-type NOS and fulllength F1395S NOS, suggesting interplay between CaM binding, nicotinamide binding and F1395 in coenzyme binding/FAD ... approach in studying the isolated FAD domain of nNOS has enabled us to analyse in detail the effects of these mutations in the absence of other in uences (most notably those induced by CaM binding ... [35,37]) In particular, domain dissection has facilitated precise determination of the midpoint reduction potentials of flavin cofactors, and also of the haems in the case of NOS and P450 BM3 In spectroelectrochemical...

Ngày tải lên: 30/03/2014, 14:20

... forebrain cholinergic neurons [57] and direct TNFα administration in the brain of mice results in an impairment of basal forebrain cholinergic function [58] However, whether induction of iNOS and ... cytokines of the inducible nitric oxide synthase promoter in insulin-producing cells Diabetologia 1998, 41:1101-8 Kong GY, Peng ZC, Costanzo C, Kristensson K, Bentivoglio M: Inducible nitric oxide ... TNFα in the presence A: absence blots detecting iNOS in total NGF in A: Western blots detecting iNOS in total protein extracts from PC12 cells treated for 24 hr with increasing concentrations of...

Ngày tải lên: 19/06/2014, 22:20

... forms of nitric oxide synthase in autoimmune encephalomelitis 27 28 29 30 31 32 33 34 35 36 37 38 Induction of nitric oxide synthase activity in human astrocytes by interleukin-1beta and interferon-g ... iNOS may be involved in the process of apoptosis [40] In conclusion, our results showed that the three isoforms of NOS including nNOS, eNOS, and iNOS were increased in the initiation of EAE and ... expression of both nNOS and eNOS in the spinal cords of rats with EAE, small amounts of iNOS were identified in the normal spinal cords but its expression slightly increased in the spinal cord of 5CFAtreated...

Ngày tải lên: 07/08/2014, 14:22

... CaM-binding peptide reported in [21] Amino acids in bold represent conserved residues in the respective CaM-binding domains CaM-binding domain CaM amino acids in contact with eNOS peptide eNOS iNOS ... containing the N-terminal EF hand pair produced higher yields of iNOS, indicating better protection of the CaM binding region than provided by the C-terminal EF pair Visible spectra of iNOS kDa ... studies on the structures of peptides derived from the calmodulin-binding domains of inducible and endothelial nitric- oxide synthase in solution and in complex with calmodulin J Biol Chem 272, 23050–23056...

Ngày tải lên: 16/03/2014, 13:20

... release of the synthase from its association with caveolin-3, a protein that maintains eNOS in an inhibited state [14,15] HSP90 can also favour the insertion of the heme group in the synthase ... calpain of both NOS by protecting the cleavage of the peptide bond close to the CaM binding site This explains why both NOS are inactivated by calpain in the absence of HSP90 The removal of the ... Bree P & Rabelink TJ (2003) Involvement of the proteasome in activation of endothelial nitric oxide synthase Life Sci 73, 2225–2236 Musial A & Eissa T (2001) Inducible nitric- oxide synthase is...

Ngày tải lên: 23/03/2014, 07:20

... groups were ip injected with L-NAME, a NO synthase inhibitor, at a dose of 10 mg/kg in saline, 60 minutes before the beginning of the bleeding period The doses and timing for L-NAME administration ... according to previous data from the literature [33,23,25] One hour after the beginning of the intravenous infusion of saline, and 15 after the administration of 300 IU of heparin, rats were continuously ... Nω-nitro-L-arginine methyl ester at a dose of 10 mg/kg in saline, 60 minutes before the beginning of the bleeding period) The right upper panel (III) shows the pulsatile pattern of prolactin in rats...

Ngày tải lên: 10/08/2014, 09:20

Ngày tải lên: 13/08/2014, 08:21

... the inhibition of inducible NOS expression by L-NAME administration prevented an increase in nitrogen intermediates and GABA release, but not in glutamate release [41] Also, recent results indicate ... anesthetized by intraperitoneal injection of pentobarbital sodium (45 mg/kg b.w.) before intrahippocampal administration of the following: (1) control group (n = 8) treated with 10 μl of 0.9% saline solution; ... Discussion The injection of AlCl3 into the CA1 sector of the rat hippocampus resulted in significant increases in nitrite levels, ∙O2 production, MDA concentrations and GSH contents in the forebrain cortex...

Ngày tải lên: 07/08/2014, 23:22

... localization of iNOS to apical domains of intestinal epithelial cells Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, ... which, in intestinal cells, could be bacteria present in the intestinal lumen This hypothesis is supported by positioning of iNOS on the apical side of intestinal crypts Recently PDZ-binding a2 ... association of nitric oxide synthase- 2 with Rac isoforms in activated murine macrophages Am J Physiol 281, F326–F336 Darling KEA & Evans TJ (2003) Effects of nitric oxide on Pseudomonas aeruginosa infection...

Ngày tải lên: 19/02/2014, 16:20

... Schotanus K, Steinbusch HW, Schoffelmeer AN, Berkenbosch F: Gradual inhibition of inducible nitric oxide synthase but not of interleukin-1 beta production in rat microglial cells of endotoxin-treated ... Nakasono I: Induction of nitric oxide synthase by traumatic brain injury Forensic Sci Int 2001, 123:142-149 Endoh M, Maiese K, Wagner J: Expression of the inducible form of nitric oxide synthase ... DJ: Norepinephrine suppresses inducible nitric oxide synthase activity in rat astroglial cultures J Neurochem 1993, 60:1945-1948 Feinstein DL: Suppression of astroglial nitric oxide synthase...

Ngày tải lên: 19/06/2014, 22:20

... was recognized in neurons, some inflammatory cells in perivascular cuffings Counterstained with hematoxylin, Magnification: ×132 D: Immunostaining of neuronal NOS in brain sections of goats with ... observed in inflammatory microabscesses Counterstained with hematoxylin Magnification: ×132 C: Immunohistochemical staining of inducible NOS in the caprine brain with natural listeriosis shows that iNOS ... of nNOS and iNOS in the nonneurological control brain Both nNOS and iNOS immunoreactivity was recognized in the brain of control goats No inflammatory cells were found in the brain sections of...

Ngày tải lên: 07/08/2014, 14:23

Ngày tải lên: 07/08/2014, 15:20

... Figure Janus kinase (JAK)2 inhibition blocks leptin/IL-1-induced nitric oxide (NO) production and nitric oxide synthase (NOS) type II protein expression expression Effect of tyrphostin AG490 and ... accumulation in the culture supernatant of human primary chondrocytes (Fig 1c) and ATDC5 cells (Fig 1d) Janus kinase-2 inhibition blocks leptin/IL-1 induced nitric oxide production and nitric oxide synthase ... were incubated for 48 hours with both cytokines in the presence of the NOS type II inhibitor aminoguanidine (1 mmol/l), added hour before cytokine administration Aminoguanidine completely inhibited...

Ngày tải lên: 09/08/2014, 06:22

... coworkers [70] in A549 cells (human alveolar type II lung carcinoma cell line) stimulated by R922 a mixture of cytokines containing interferon-γ, TNF-α and IL-1β Inhibition of iNOS by actinomycin and ... ankyrin repeat containing a serine/threonine protein kinase that interacted with the cytoplasmic domain of β1 integrin [51] Furthermore, Liu and Lioté [37] found that, within a few minutes, MSU crystals ... protein kinases and tyrosine kinases on IL1-induced NF-κB activation and iNOS expression in bovine articular chondrocytes Nitric Oxide 2002, 6:35-44 66 LaPointe MC, Isenovic E: Interleukin-1β...

Ngày tải lên: 09/08/2014, 06:23

... mins B: Representative gels (inset) and quantified data showing changes in the protein level of active form PKG-I in NTS of the rat at 30 after receiving bilateral microinjections into NTS of ... Neuronal nitric oxide synthase activation is involved in insulin-mediated cardiovascular effects in the nucleus tractus solitarii of rats Neuroscience 2009, 159:727-734 Prast H, Philippu A: Nitric ... Ser1416 [21] As illustrated in Figure 3A, ADM induced a significant increase in protein levels of phospho-nNOS (Ser1416) in NTS The ADM-induced increase in phosphorylation of nNOS at Ser1416 maximized...

Ngày tải lên: 10/08/2014, 05:21