the zinc finger protein egr 1

... G→T GCCTG(G/T)GCGAG G1 31 C 5 /11 8 (4.2%) ZFP36 *12 PCD (ex.2) 211 2 C→T GCCTT(C/T)TCTGC F232→F 4/97 (4 .1% ) ZFP36 *13 PCD (ex.2) 218 4 C→A AGGGC)CA_ACTCC A256→A 10 /94 (11 %) ZFP36 *15 PCD (ex.2) 3059 A→C ... [23] The arrow indicates the migration position of the ARE probe (lanes 1 6) and the deadenylated product of probe ARE-A50 (lanes 9, 11 , 12 , 14 , 16 and 17 ) (b) The extracts used in lanes 7 13 of ... zinc finger mutant (C124R), CMV.cMG1.tag (cMG1), CMV.mTis11D.tag (mTis11D), 0 .1 µg of human poly(A) exonuclease (hPARN) expression plasmid CMV.hPARN.flag (hPARN), or plasmid DNA alone (BS+) The...

Ngày tải lên: 09/08/2014, 01:24

... NZAP-Zeo -actin B 63 13 83 93 10 3 11 3 Nm63 Nm3 Nm13 Nm93 C SIN titer (LOG pfu/ml) Nm83 Fold inhibition Nm133 Nm103 13 23 53 63 83 93 10 3 11 3 13 3 15 3 WT 18 16 14 12 10 ZAP mutant Tet Nm 113 254 NZAP-Zeo ... Retrovirology 2 010 , 7 :19 http://www.retrovirology.com/content/7 /1/ 19 Fold inhibition A Page of 10 0 90 80 70 60 50 40 30 20 10 NZAP-Zeo mutant 13 23 43 53 63 83 93 10 3 11 3 12 3 13 3 15 3 16 3 18 3 203 223 ... 2003, 77 :11 555 -11 562 Guo X, Ma J, Sun J, Gao G: The zinc- finger antiviral protein recruits the RNA processing exosome to degrade the target mRNA Proc Natl Acad Sci USA 2007, 10 4 :15 1 -15 6 Buttner...

Ngày tải lên: 12/08/2014, 23:23

... evolution of the Prdm9 speciation gene across diverse metazoan taxa PLoS Genet 2009, 5:e1000753 doi :10 .11 86/gb-2 010 -11 -2 -10 4 Cite this article as: Neale MJ: PRDM9 points the zinc finger at meiotic ... hotspots On the basis of the wider (30-40 bp) context flanking the 13 bp motif, and of its apparent bp periodicity, these authors proposed that the motif was likely to be bound by a zinc- finger protein ... Neale Genome Biology 2 010 , 11 :10 4 http://genomebiology.com/2 010 /11 /2 /10 4 modification: Prdm9 is expressed during early meiosis; its disruption...

Ngày tải lên: 09/08/2014, 20:21

... replaced by the acetate moiety The protein coordinates zinc with the Nd atom of His12, one of the Od atoms of Asp15 and the Ne atom of His 113 His 113 protrudes from helix a4 whereas His12 and Asp15 both ... with the same residues coordinating a zinc ion These residues plus an additional conserved motif (Fig 7B) in the immediate neighborhood of the active site (His 110 , Pro 111 , Asp 112 , His 113 in the ... with the protein encoded by bc1534 [4,5] Furthermore, bc1534 also has a homologue in the B cereus genome, the bc34 61 gene, with 25% identity, at the amino acid level The protein encoded by bc1534...

Ngày tải lên: 19/02/2014, 00:20

... 64, 314 – 319 Zinc- binding protein in the sea urchin 20 Brooks JM & Wessel GM (2002) The major yolk protein in sea urchins is a transferrin-like, iron binding protein Dev Biol 245, 1 12 21 Yokota ... part of the zinc in the immature gonads is bound to MYP The elution patterns of zinc were similar in the ovary and testis, but the zinc peak in the immature ovary was larger than that in the immature ... 43 .1 ± 1. 7% of the MYP was CFMYP at stage and 18 .3 ± 2.6% at stage In the testes, the proportions of CFMYP were 17 .7 ± 5.3% at stage and 6.9 ± 3.5% at stage The higher the proportion of CFMYP, the...

Ngày tải lên: 16/03/2014, 05:20

... 10 1, that GAMyb is a GA-regulated transcription factor 18 1 19 1 required for transcriptional activation of the high-pI Ó FEBS 2004 A new zinc- finger protein from rice (Eur J Biochem 2 71) 2955 15 ... in the protein This His192 abolished the binding of the protein to the O2S conclusion is supported by the observations that the RAMY sequence completely 2954 R Peng et al (Eur J Biochem 2 71) ... sites of the flanking the GAL4 activating domain and the ADC1 vector pALEX Ó FEBS 2004 A new zinc- finger protein from rice (Eur J Biochem 2 71) 29 51 32 Fig The rice RAMY cDNA sequences and the predicted...

Ngày tải lên: 16/03/2014, 18:20

... Journal 277 (2 010 ) 42 41 4253 ª 2 010 The Authors Journal compilation ª 2 010 FEBS 42 51 ´ I Frechette et al CUX1 represses PLZF transcriptional activity 10 11 12 13 14 15 16 17 18 19 20 gene expression ... p200Cux1 (e.g p 110 Cux1) as well as alternative splicing (e.g p75Cux1) [12 ] Many in vitro gene targets of CUX1 are implicated in the control of the cell cycle [10 ,13 ,14 ] Genetic deletion of Cux1 in ... validated for the absence of CUX1 interaction did not compete, as illustrated for sites 8, 10 , 12 , 13 , 14 and 15 in Fig 2C The addition of affinity-purified polyclonal CUX1 antibodies to the binding...

Ngày tải lên: 29/03/2014, 21:20

... ớt hn so vi nóo (12 , 22) (Logothesis et al., 19 88) Cỏc dimer 12 v 13 cú kh nng kớch thớch hot tớnh ca adenylate cyclase kiu II mnh gp 10 ln so vi 11 (Iniguez Lluhi et al., 19 92) Cỏc tiu n v ... C (Blacklund et al., 19 90; Fung et al., 19 90; Maltese v Robinshaw, 19 90; Mumby et al., 19 90a; Yamane et al., 19 90; Sanfod et al., 19 91) Nhúm farnesyl 15 C trờn (Lai et al., 19 90) ớt k nc hn nhúm ... et al., 19 87) Mt vi khỏng th iu khin chng li cỏc peptit vựng u C cng khụng ghộp cp vi th th t protein G (Simon et al., 19 89; Gutowski et al., 19 91) Peptit photorhodopsin i din cho 11 gc cui...

Ngày tải lên: 23/10/2013, 18:20

... nuôi 10 12 CT CT CT CT 3 .11 0.91a 2.92 0.93a 2.62 0.86bc 2.85 0.84ac 4.98 1. 71a 4.66 1. 21a 4.68 1. 58a 4. 81 1. 33a 6 .14 3.66a 6.67 2.27a 6.48 2 .18 a 6.56 1. 87a 8.26 2.55b 8.60 1. 96a ... 8.26 2.55b 8.60 1. 96a 8.80 2.67a 8.89 2.55a 10 .333.22b 11 .033.07a 11 .05 3.32a 10 .94 2.94a 10 .86 3.28b 11 .5 3.34a 11 .68 4.52a 11 .67 3.47a (Theo cột nghiệm thức có chứa chữ giống sai khác ... (g/con) 41, 7 18 ,6 24 cá Hệ thống nuôi Protein (%) 31- 34 23 20-50 Bể nớc Ao nớc Bể xi măng Tác giả Hanlay, 19 91 Green, 19 92 Wee an Tuan, 19 88 35 13 ,53 300-600 8 -10 15 -20 >4 Lồng nớc 19 ,87 Ao nớc...

Ngày tải lên: 18/12/2013, 20:33

... Cao 02 4 ,10 1, 60 5,99 1, 60 8 ,10 1, 60 24 4 ,14 1, 62 6,30 1, 62 8,03 1, 62 4-6 4,34 1, 67 6,26 1, 67 8 ,14 1, 67 6-8 4,48 1, 68 6,26 1, 68 8 ,15 1, 68 - 10 4,49 1, 65 6,28 1, 65 8 ,17 1, 65 (Trong ... a a a 2,58 0 ,14 2,58 0 ,16 2,57 0 ,15 2,58a 0 ,15 10 ,34a 0, 81 10,27a 0,80 10 ,24a 0,82 10 ,11 a 0,95 18 ,46a 2,89 18 ,22a 2,22 18 ,00a 2 ,12 17 ,93a 2,23 25,06a 4,49 24,79a 4, 31 24,70a 4,38 ... trờng nớc .12 1. 3 Chất lợng nớc yếu tố môi trờng ảnh hởng tới cá Rô phi 12 1. 3 .1 Nhiệt độ 12 1. 3.2 Oxy hoà tan (DO) 13 1. 3.3 Độ pH 13 1. 3.4 Hyđrosulfide...

Ngày tải lên: 18/12/2013, 20:56

... DAPK -1 gene Its coding region starts from the 10 th base pair on exon 15 of the DAPK -1 gene, and shares the same splicing as full-length DAPK -1 through the rest of exons 15 , 16 , 17 , 18 , 19 and ... s-DAPK -1 s coding region stops at the 12 6th base pair of intron 20– 21 of the DAPK -1 gene, and the 3¢-UTR extends through the middle of intron 20– 21 (B) Comparison of the protein sequences of DAPK -1 ... underlying the bleb formation [11 ] Furthermore, microtubule-associated protein 1B interaction with the kinase domain of DAPK -1 stimulates membrane blebbing and autophagy [12 ] The roles of its other...

Ngày tải lên: 18/02/2014, 17:20

... human genome Cell 11 7, 699– 711 14 Van Vactor D (19 98) Protein tyrosine phosphatases in the developing nervous system Curr Opin Cell Biol 10 , 17 4 18 1 15 Stoker A & Dutta R (19 98) Protein tyrosine ... UK) in lL of 10 mm NH4HCO3 The pieces were then overlaid with 10 mm NH4HCO3 (10 lL) and incubated for 16 h at 37 °C The samples were centrifuged at 16 000 g on a Heraeus Biofuge and the supernatant ... 22, 215 9– 217 0 O’Grady P, Thai TC & Saito H (19 98) The laminin– nidogencomplex is a ligand for a specific splice isoform of the transmembrane protein tyrosine phosphatase LAR J Cell Biol 14 1, 16 75 16 84...

Ngày tải lên: 19/02/2014, 05:20

... Tyr 311 , Tyr 316 , Tyr330 or Tyr3 31, for the five tyrosine-phosphorylated peptides, ephrinB2(3 01 333)-pY304, ephrinB2(3 01 333)-pY 311 , ephrinB2(3 01 333)-pY 316 , ephrinB2(3 01 333)-pY330 and ephrinB2(3 01 333)-pY3 31 ... and each of the other five peptides (i.e ephrinB2(3 01 333), ephrinB2(3 01 333)pY 311 , ephrinB2(3 01 333)-pY 316 , ephrinB2(3 01 333)pY330 and ephrinB2(3 01 333)-pY3 31) were larger than 500 lM These results ... (m) and YEEI-pY304 (r) (Fig 3) EphrinB2(3 01 333) series WT pY304 pY 311 and pY 316 pY 311 or pY 316 pY304 and pY 311 pY304 and pY 316 pY330 or pY3 31 EphrinB2(3 01 309) series YEKV-pY304 YEEV-pY304 YEKI-pY304...

Ngày tải lên: 19/02/2014, 12:20

... 20:4 18 :1, 18 :2 + 18 :0, 22:6a + 16 :1, 18 :1a 16 :0, 18 :2 + 18 :0, 18 :3a 16 :0, 16 :1 18:0, 22:5 18 :0, 20:4 17 :0, 18 :2 + 16 :1, 17 :0a 18 :1, 18 :1 16:0, 18 :1 + 18 :0, 18 :2a 16 :0, 16 :0 + 18 :0, 22:4a 18 :0, 18 :1 ... species 16 :1, 20:4 18 :2, 20:4 + 16 :0, 20:5a 16 :2, 18 :2 18 :2, 18 :2 + 18 :1, 18 :3a + 16 :1, 20:3a 16 :1, 18 :2 + 16 :0, 22:6a + 14 :0, 14 :0a 16 :1, 16 :1 + 14 :0, 18 :2a + 14 :0, 16 :1a 16 :1, 22:4 + 18 :0, 20:5a 16 :0, ... PKCf-immunoprecipitate 95 .1 148 218 .5 249 .1 91. 6 17 1.7 16 1.4 18 8.3 15 1.5 19 9.7 ND ND ND ND ND ND ND 2.26 ± 0 .14 ND 2 .13 ± 0. 21 ± ± ± ± ± ± ± ± ± ± 10 34 19 d 40c 15 29 18 40 14 29 Assay with Ca2+ and the epidermal...

Ngày tải lên: 20/02/2014, 02:21

... pGAD10.Ikaros2 Other plasmids are pGAD10.Eos1 01 5 31, pGAD10.Eos1 01 5 31. mut, pGAD10 Eos1 01 364, pGAD10.Eos1 01 3 31, pGAD10.Eos1 01 2 31, pGAD10.Eos364– 518 , pGAD10.Eos364– 518 .mut, pGAD10.Ikaros1– 81, pGAD10.Ikaros1– 81. mut, ... - 1. 0µg - 1. 0µg - 0 .1 g + 81 81 X 0 .1 g 81 81 Ikaros 10 15 Fold repression DISCUSSION B Gal4DBD CtBP + 0.5µg - 1. 0µg 518 364 518 364 - 1. 0µg X 364 364 - 518 0 .1 g + 518 0 .1 g Eos 10 15 20 Fold ... CtBP + - 1. 0 µg - 1. 0 µg - 0 .1 g + Gal4DBD 0.5µg 0 .1 g 10 68 12 81 1068 X 12 81 1068 12 81 1068 12 81 TRPS1 10 20 30 40 50 Fold repression Fig CtBP binding domains of Ikaros, Eos and TRPS1 act as...

Ngày tải lên: 21/02/2014, 01:21

... of LPS alone (10 mgÆmL )1) or IFN alone (10 0 UÆmL )1) induced the NO productions but were very feeble as 1. 1-fold and 1. 4-fold of control, respectively These results indicate that the detectable ... in VSMC The concentrations of LPS and IFN were 10 lgÆmL )1 and 10 0 UÆmL )1, respectively (A) NO was measured using the Griess reagent as nitrite The amount of TS added alone was 10 lM The doses ... of 10 lgÆmL )1 and 10 0 UÆmL )1 were also added to the dishes for induction of NO production Then, 48 h after the addition of LPS/IFN, the cells were subjected to various assays Nitrite analysis The...

Ngày tải lên: 22/02/2014, 04:20

... Journal 278 (2 011 ) 17 79 17 89 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 17 87 Interaction of TnrA with GlnK and GS A Kayumov et al were used Initially, the proteins (10 nm each protein) were ... Pi (4.3 mm Na2HPO4, 1. 8 mm KH2PO4, 13 7 mm NaCl, 2.7 mm KCl, pH 8.0) The beads were harvested by short centrifugation (11 500 g, 30 s, FEBS Journal 278 (2 011 ) 17 79 17 89 ª 2 011 The Authors Journal ... 2-oxoglutarate [12 ] Other nucleotides, such as ADP, AMP, and GTP, at concentrations of FEBS Journal 278 (2 011 ) 17 79 17 89 ª 2 011 The Authors Journal compilation ª 2 011 FEBS A Kayumov et al 1 3 mm had...

Ngày tải lên: 06/03/2014, 00:21

... (2 010 ) 14 53 14 64 ª 2 010 The Authors Journal compilation ª 2 010 FEBS ` P Lores et al 10 11 12 13 14 15 16 17 18 19 20 Identification of SWI.SNF complex subunit BAF60a as a determinant of the transactivation ... myc-tagged GTPases RhoA, Rac1 and Cdc42, either wild-type (Rac1WT and Cdc42WT), inactive mutant form (Rac1N17) or activated mutant forms (RhoAL63, Rac1L 61, Cdc42L 61) .The expression level of GTPases ... Journal 277 (2 010 ) 14 53 14 64 ª 2 010 The Authors Journal compilation ª 2 010 FEBS 14 61 ` P Lores et al BAF60b ubiquitination is controlled by Rac and Unkempt For western blotting, the secondary...

Ngày tải lên: 06/03/2014, 09:22

... Subramanian et al D108 -1 (HMGN2–/–) Nh43 (HMGN1a–/–/N2–/–) Bp5 (HMGN1a–/–/N2–/–) UV– Propidium iodide Propidium iodide 10 000 10 00 10 0 10 10 000 UV+ 10 00 10 0 10 10 10 0 10 00 10 000 Annexin V–FITC ... G2-M BrdU G1-G0 45.6 14 .6 32.6 27.3 18 .9 22.4 10 000 27.5 10 00 11 .3 16 .8 UV+ 10 0 10 200 22.7 33.9 31. 0 600 400 200 400 21. 1 53.6 600 200 57.5 400 600 Propidium iodide B 70 c G1-G0 G2-M 40 f a b ... cells 60 e c 20 10 DT40 C DT40 D108 -1 D108 -1 Nh43 + UV + UV Nh43 + UV n UV mi h h h h h No 30 10 24 48 72 DT-40 D108 -1 Nh43 Phospho-Chk1 Ser 345 DT-40 D108 -1 Nh43 DT-40 D108 -1 Fig UV-induced...

Ngày tải lên: 07/03/2014, 00:20

... [3,4 ,12 16 ] Moreover, Tec and other members of this group show a high degree of conservation across species [11 ,17 ,18 ] Previous studies have shown that Tec plays important roles in regulating the ... lengths of the mouse Tec promoter were generated (Fig 1A) To determine the activity of the Tec promoter, the reporter constructs were introduced into the B-cell line A20 As shown in Fig 1C, the shortest ... H (19 97) Tec protein- tyrosine kinase is involved in the thrombopoietin ⁄ c-Mpl signaling pathway Exp Hematol 25, 211 – 216 27 Honda H, Ozawa K, Yazaki Y & Hirai H (19 97) Identification of PU .1 and...

Ngày tải lên: 07/03/2014, 00:20