the teratoma assay an in vivo assessment of pluripotency

... as the binding constants and the extent of autoregulation The regulatory proteins may reside either in the nucleus or in the cytoplasm or in both, thus controlling the network The existence of ... model provide insights into the importance of their roles in the overall performance of the network A similar experimental evaluation of the roles of individual elementary mechanisms in vivo is very ... mechanism that controls the protein expression in the wild-type is the shuttling of Gal80p Increasing the amount of Gal80p in the cytoplasm by increasing the distribution coefficient (K) causes the...

Ngày tải lên: 07/03/2014, 16:20

... harvested, cleaned of soft tissue, and fixed in 70% ethanol The femur bone was trimmed above and below the ends of the implant, cleaned of soft-tissue, and fixed in 70% alcohol The specimen was further ... developed intestinal obstruction after ingesting surgical dressing and was euthanized days before schedule The femora were harvested from this rabbit and included in the SEM analysis The details of ... (with an acid resistant mask) and chemical milling with nitric and hydrofluoric acid The details regarding the chemical texturing process and the osseointegration of chemical-textured implants...

Ngày tải lên: 20/06/2014, 04:20

... in the delineation of the brachytherapy catheters during treatment planning due to partial volume effects in the planning CT Retraction of the catheters within the catheter tracks during transfer ... from the coordinates of the catheter skin entry site and the catheter tip in the treatment planning CT If more than one catheter was implanted, an average MA+ Lesion Page of 10 coordinate from the ... mm and of interstitial edema (T2-w images) -5 ± 6.0 mm in plane, indicating a shift of the irradiation effect volume against the corresponding tolerance dose volume in the direction of the catheter...

Ngày tải lên: 09/08/2014, 09:21

... involved in joint inflammation during an acute response that may offer insight into early proinflammatory cytokine release in the initial phases of the inflammatory response This is often lost by using ... per the manufacturer's instructions Blots were scanned and analyzed for band intensities by using UN-SCAN-IT version 5.1 software (Silk Scientific, Orem, UT, USA) Transient transfection of human ... killed, and joints and serum were collected at 24 and 48 hours after zymosan administration Only hind joints were used in the study Joints were removed directly below the hairline and snap frozen in...

Ngày tải lên: 12/08/2014, 14:22

... Science Council in Taiwan (grant NSC94-2320-B-009-003) and the Bureau of Animal and Plant Health Inspection and Quarantine Council of Agriculture in Taiwan (grants 95AS-13.3.1-BQ-B1 and 95AS-13.3.1-BQ-B6) ... microscopy can detect GNPs by measuring this enhancement Ex vivo diffusion of GNPs into liver tissues was also performed to verify the enhancement of the Raman signal Localized enhancement of the anti-Stroke ... diffusion into the liver, lung, and spleen 123 862 Fig ELISA of GNPs using anti-endotoxin IgG ELISA was performed by using anti-endotoxin IgG against various sizes of GNPs synthesized in the lab...

Ngày tải lên: 22/06/2014, 00:20

... among the five strains There are nine unique amino acids in the RNase/ RT of BLV ARG 38 relative to all of the other BLV strains, including BLV ARG41 There are no differences between the integrases ... promoters of RNA transcription, the polyadenylation site (PAS), the CAP site and the tRNA proline primer binding sites are as shown The env leader peptides and the transmembrane gp30 env proteins of ... ends of the various proteins are indicated by the up arrow Stop codons are indicated by asterisks Only a portion of the BLV A Tax is shown, and the rest is indicated by the an wavy lines In the...

Ngày tải lên: 12/08/2014, 04:21

... among the five strains There are nine unique amino acids in the RNase/ RT of BLV ARG 38 relative to all of the other BLV strains, including BLV ARG41 There are no differences between the integrases ... promoters of RNA transcription, the polyadenylation site (PAS), the CAP site and the tRNA proline primer binding sites are as shown The env leader peptides and the transmembrane gp30 env proteins of ... ends of the various proteins are indicated by the up arrow Stop codons are indicated by asterisks Only a portion of the BLV A Tax is shown, and the rest is indicated by the an wavy lines In the...

Ngày tải lên: 12/08/2014, 04:22

... overall incidence of PTLD is - 2% The incidence of the disease in renal and liver transplant is – 5%, in heart and heart-lung transplant is – 15%, and in intestinal transplant is 10 – 15% 125 The incidence ... with the type of organ transplanted, the age of the patient and the intensity of the immunosuppressive regime used The highest rate of PTLD is seen in the first year post- transplant124 The overall ... cells in solid organ transplant patients and from recipient cells in bone marrow transplant patients A hypothetical role of latent EBV proteins in the pathogenesis of PTLD has been explained in the...

Ngày tải lên: 10/09/2015, 09:22

... interpretation of the factors and then processing of the completed questionnaire and reporting The second part of the work also includes the validation and discussion on the results obtained Scheme of the ... for Individual Linkage 4.4.1 The Goal The goal of the single problem is primarily considered to identify the strength of the individual linkage by determining the relative importance of the linkage ... Different Characteristics of University and GRI units 13 3.1 An example of in and out flow of factors in the innovation triangle linkage 21 3.2 Type of Linkages in the Innovation Triangle 23 4.1 Budget...

Ngày tải lên: 23/04/2013, 10:29

... containing the helper plasmid pKD46 Transformants were incubated h at 37 °C and overnight at room temperature in SOC medium and then plated on Luria–Bertani agar plates containing kanamycin Kanamycin ... Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was collected and used ... between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing determined amounts of the purified UP12 were separated by SDS/PAGE along with the cell...

Ngày tải lên: 22/02/2014, 07:20

... take the survey but, because of the small number of diabetic patients that we can find within a facility (39 in our case) and the difficulty of repeating coordinated activities of this type in the ... bearing in mind that some inmates had to abandon the study due to different reasons Recruiting them afterwards for the study would have meant misrepresenting the results as a result of the inmates ... studies indicate that social inequality can be responsible of increases in the prevalence of some diseases such as DM2 while, on the other hand, the provision and quality of primary care services can...

Ngày tải lên: 05/03/2014, 22:21

... turnover of airway dendritic cells is in uenced by the in ammatory state of the lungs [24] and that these cells play an essential role both in the induction and maintenance of allergen-driven eosinophilic ... that the clearance and further metabolism of the allergen was altered as a result of the in ammation in the lungs of sensitized animals Up to now there are few data available on the fate of an ... proportion of radioactivity was detected in the lungs of sensitized mice than in nonsensitized animals The distribution of radioactivity in the other investigated organs did not differ due to the sensitization...

Ngày tải lên: 07/03/2014, 21:20

... 1) are on the transside of the thylakoid, or that they are shielded from trypsin digestion on the cis-side of the membrane The A B 4004 Fig Determination of the topology of PSI-G in the thylakoid ... plants expressing similar levels of PSI-G, and analysed by immunoblotting, using antibodies to PSI-G and PSI-F, as an indicator of the relative content of PSI in the samples Plants lacking PSI-G ... trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D (extrinsic membrane protein,...

Ngày tải lên: 07/03/2014, 21:20

... that these inhibitory domain–domain interactions are relieved by binding of the metal to the N-terminus of the ATPase To further study the role of the Ccc2 N-terminus in the overall function of the ... [36,37] The current hypothesis involves domain–domain interactions between the N-terminus and other domains of the ATPase Indeed, in vitro studies of the purified N-terminus and catalytic loop of the ... that these two domains interact in the absence of copper and that their interaction is diminished by copper binding to the N-terminus [38] Such a domain–domain interaction has been proposed to hinder...

Ngày tải lên: 23/03/2014, 05:22

... calpain degradation Results Levels of HSP90 and NOS isozymes in rat brain and aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting ... provide a new insight into the understanding of the mechanisms involved in modulating NO production In such a case, the availability of adequate amounts of HSP90 becomes the limiting factor Our ... quantitative by the use of known amounts of proteins submitted to SDS-PAGE and staining with the appropriate antibody The bands were then scanned, and the areas of the peaks obtained were used to create...

Ngày tải lên: 30/03/2014, 04:20

... reported as the final molar concentration in the organ bath Endothelium-independent vasorelaxation was tested after mechanical endothelium removal of the endothelial layer Surgical Induction of Hindlimb ... and slides were counterstained with a standard mixture of hematoxylin and eosin [4] Quantitative analysis was done by counting the total number of endothelial cells, identified by lectin staining ... we examinated the in vivo effects of a VEGF165 mimetic, named QK, modeled on the region of the VEGF protein responsible for binding to and activating the VEGFRs that are known to trigger angiogenesis...

Ngày tải lên: 18/06/2014, 15:20

... Dif- Page of 13 ferently, using the anti-ErbB-2 antiserum cross reacting with the murine receptor resulted in a faint stain of the cytoplasm and rarely in staining of the membrane of the tumor ... demonstrating the inverse correlation of the expression of the two tumor relevant molecules and implying their functional link Discussion The understanding of the mechanism responsible for the in vivo ... positive staining ranging from moderate to intense staining In the context of the potential association of htid expression with the biology of human breast cancers of different subtype the immunohistochemical...

Ngày tải lên: 18/06/2014, 16:20

... the ATFL and CFL in- vivo The objective of the current study was to measure the change in apparent length of the ATFL and CFL, in- vivo, in uninjured healthy ankles during a dorsiflexion-plantarflexion ... during an inversion injury to the ankle.[23] These cadaveric studies have provided fundamental knowledge for the understanding of function of these ligaments and injury mechanisms However, the in- vivo ... Epidemiology of sprains in the lateral ankle and foot Foot Ankle Int 1994, 15(2):72-4 Freeman MA: Instability of the foot after injuries to the lateral ligament of the ankle J Bone Joint Surg Br...

Ngày tải lên: 20/06/2014, 01:20

... increasing resonance frequencies The graph change in shape and position is more important at the beginning of the insertion when the stem displacement is important as well At the end of the insertion, ... corroborated and further elucidated by recent advanced finite element analyses by our group [25,29] During the insertion of the uncemented stem the FRF change is influenced by the stiffness of the implant-femur ... insertion stages and canal and reinserting the prosthesis The FRF had a normal evolution during the reinsertion and the graphs corresponding to the final two stages, labelled as stage 4a and stage 5a,...

Ngày tải lên: 20/06/2014, 01:20

... gastrointestinal tract of animals However, not all strains of Bacillus are equally resistant to the environment in the gastrointestinal tract and antimicrobial activity varies between strains (Chang ... through the small intestine (Huang and Adams 2004) The experimental results of Casula and Cutting (2002) suggest that B subtilis germinates in the jejunum and ileum The adverse conditions in the ... Faeces from the negative control and the diet containing spores that performed best during the performance trial were analyzed Statistical analysis In the screening experiment, the counts of viable...

Ngày tải lên: 07/07/2014, 19:20