... expression of these genes in theintestinalepitheliumofzebrafish at a level of single cell The main aim of this thesis is to produce a detailed study and analysis ofthezebrafishintestinalepithelium ... epitheliumrenewal Chapter The spatial orientation ofzebrafishintestinalepitheliumrenewalThe spatial orientation ofzebrafishintestinalepitheliumrenewal by positive marking of epithelial ... Chapter The spatial orientation ofzebrafishintestinalepitheliumrenewal 30 The spatial orientation ofzebrafishintestinalepitheliumrenewal – by positive marking of epithelial...
... μl of PBS in the left ear DTH response was calculated as the percentage swelling (the difference between the increase of thickness ofthe right ear and the left ear, divided by the thickness of ... the co-cultures The addition of these inhibitors resulted in the abrogation ofthe inhibition of Tcell proliferation by wild-type MSCs (Figure 3e) The addition ofthe IDO inhibitor 1-methyl-DL-tryptophan ... μg of CII in the right ear and vehicle in the left ear Delayed-type hypersensitivity responses were measured as the percentage of swelling (100 × [(thickness ofthe right ear – thickness of the...
... ATL, HCs – ATL) The zero value of tax gene transcripts was observed in 60.7% of HAM/TSP patients (34 out of 56), 71.1% of HCs (27 out of 38) and 30.0% of ATL patients (3 out of 10) The medians are ... at least the biclonal integration of HTLV-1 proviral DNA The incidence of multibands in our cases (two out of ten: 20%) was comparable with the data by Kamihira et al (17.8%) The Page of 11 (page ... load = value of tax/value of HPRT HTLV-1 HBZ mRNA load = value of HBZ/value of HPRT We used aliquots ofthe same standard MT-2 cDNA preparation for all assays and the correlation values of standard...
... deleted from the C terminus of GagPol Figure 1B shows Western blots of lysates ofthe viruses produced from the different transfections, probed with anti-CA, and shows that all forms of GagPol deletion ... sequences including the thumb domain of RT are deleted (∆581 and ∆715) To measure the amount of tRNALys3 annealed in vivo to the viral RNA genome, total viral RNA was used as the source of primer/template ... Figure 2B The left side of panel A shows that there is a linear increase in the reverse transcription signal over an almost 10 fold change in the amount of BH10.P- viral genomic RNA used in the reac-...
... monitoring the therapeutic effect of HSV-TK, U87MG cell clones stably expressing the HSV-TK-Luc fusion protein were generated by selection of transfected cells with geneticin Comparison of 18 of these ... mouse prior to sacrifice The tumor was cut in the middle and placed with the cut side facing the CCD camera One part ofthe tumor was obviously hemorrhagic while the other part looked vital Photon ... conversion of prodrug by HSV-TK was however not measured in either ofthe above studies Ponomarev et al [24] did not present data on the correlated expression ofthe three reporters within the triple...
... major contributor for the clearance of NPs, thus the reducing rate of MPS uptake could be considered as the best strategy for prolonging the circulation of NPs (Li & Huang, 2008) Therefore, PA/EPI ... respectively The bioavailability of free EPI was 100%, the BAR of PA/EPI calculated through the equation above by the AUC0–24h was 189%, much higher than the free drug According to the previous ... toxicity ofthe PANs was found in the experimental animals after i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI...
... comparison, the amount of GroEL was determined in the same extracts using anti-GroEL Ig It has been previously shown that at the beginning ofthe stationary phase the rate of synthesis of heat-shock ... respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing determined amounts ofthe purified UP12 were separated ... Taken together, these observations indicate that maintenance ofthe correct folding state of GatY in E coli probably requires the assistance of two chaperone systems The identification of UP12 as...
... PP The concentrations of porphyrins used in the growth medium are given in Table In the case of Ru-meso, a low concentration had to be used because ofthe toxicity of this compound Production of ... suggested that the metal ion ofthe porphyrin is removed during transport ofthe porphyrin from the medium to the catalase protein in the cytoplasm Alternatively, but less likely, the metalloporphyrin ... 700 nm (C) Spectra of catalases produced in the presence of Cu-PP, Mg-PP, and PP The intensities of these spectra have been normalized with respect to the Soret band absorption The inset in (C)...
... constrain the operation ofthe network However, the response for a specific system is constrained by the connection between these elements The response is also influenced by the relative dominance of these ... as the binding constants and the extent of autoregulation The regulatory proteins may reside either in the nucleus or in the cytoplasm or in both, thus controlling the network The existence of ... example, the operation ofthe switch is constrained by the number of binding sites and the amounts of regulatory proteins Further, numerous elements such as stoichiometry (dimerization), number of...
... modifications of chromatin [9] Little is known, however, ofthe functional role ofthe remaining subclass members In this study, the yeast protein SPP1 (alias cps40), a member ofthe COMPASS complex ... constitute CGBP homologues The presence of a TFS2M domain is also indicative of a possible function for the DIDO1 long isoform (Figs and S2) It is the second domain ofthe elongation factor, TFSII ... in the tree (Fig 1), the branch ofthe FEBS Journal 272 (2005) 3505–3511 ª 2005 FEBS A M Rojas et al fungal sequences is clearly distant from the other branches, which was indeed predicted The...
... from the administered [75Se]Der p2, indicate together with its own remaining intact levels (the 16 kDa band) that the clearance and further metabolism ofthe allergen was altered as a result ofthe ... conclude that the inflammatory state ofthe lung influence on the rate of metabolism and clearance of Der p Thus, an allergic response may lead to prolonged retention ofthe allergen in the airways ... detected in the lungs of sensitized mice than in nonsensitized animals The distribution of radioactivity in the other investigated organs did not differ due to the sensitization Thus, the allergen-induced...
... of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G in the transformed knock-out line does not reach the level ofthe ... topology where the loop is in the thylakoid lumen, and the N-and C-termini face the stroma, opposite to that of PSI-K On the other hand, the second scenario implies a stromal loop, like that of PSI-K, ... integration ofthe subunit into the PSI complex A protein band smaller that the tagged versions of PSI-G is present in the lanes with the loop-tagged PSI, but is also seen faintly in the lanes with the...
... A-deficient rats In the liver of rats with hypervitaminosis the results showed a decrease in the amount of p53 and an increase ofthe c-Jun The figure shows that the expression of p53 is time course ... analyzed by the Levene test; in those cases in which the variances were unequal, the data were adequately transformed before the ANOVA The null hypothesis was accepted for all the values of these sets ... explain the importance of keeping vitamin A ofthe incidence of cancer, cardiovascular disease, or death status within the normal range, because p53 tumor 21 from all causes [37] The failure of these...
... effects of EBI compounds on the plasma membrane H+-ATPase of U maydis Furthermore, the utilization of two different targets in the same biosynthetic route permitted us not only the confirmation ofthe ... in the indirect effects of sterol modification, namely, the activation of plasma membrane H+-ATPase through changes in the fatty acid profile There have been numerous reports on the influence of ... sterols plus, in the case of EBI-treated sporidia, other collateral effects of these compounds [33] In U maydis, changes in the plasma membrane 18 : 2/18 : ratio followed the inhibition of growth rate,...
... the fluorescence dye from the quenching molecule by the Dicer enzyme, which cleaved the synthetic let-7 precursor miRNA (Fig 1D) [19] In the absence ofthe Dicer protein, the emission energy of ... present there is cleavage ofthe end ofthe pre-miRNA, and activated fluorescence signals are observed, which implies that the fluorescence beacon system is useful for the detection ofthe functional ... abnormalities occurring during each ofthe steps of miRNA biogenesis Second, the oncogenic characteristics ofthe miRNAs regulate the progression of cancer by preventing the tumor suppressor genes from...
... aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially expressed isoform, together ... occurred in the brain of NMS rats, although a small fraction ofthe native 160 kDa synthase was converted into the still active 130 kDa form (Fig 4A) The level of HSP90 remained unchanged during the ... following the addition of IgG1-HSP90 antibody to crude extracts of brain or aorta, NOS was immunoprecipitated, indicating a specific association ofthe two proteins We then determined the amount of each...
... daily The concentration is reported as the final molar concentration in the organ bath Endothelium-independent vasorelaxation was tested after mechanical endothelium removal ofthe endothelial ... http://www.translational-medicine.com/content/7/1/41 Figure Diagram 3of the kinetics of wound closure (A) Diagram ofthe kinetics of wound closure (A) VEGF165 and QK accelerate the closure of full thickness punch biopsy wounds ... published by the National Institutes of Health in the United States (NIH Publication No 85-23, revised 1996) and approved by the Ethics Committee for the Use of Animals in Research of "Federico...
... to the logarithm ofthe initial amount ofthe target DNA in the sample The relative concentration of one target with respect to another is reflected in the difference ofthe cycle number, the ... point of view very attractive, the analysis ofthe correlation ofthe expression levels ofthe two forms L and I in the tumor samples not support it at present Decrease in the level ofthe L ... sample) The SD of ΔΔCt is the same as the SD ofthe ΔCt The ΔCt values of both the tumor and normal samples are standardized to HGPRT used as endogenous control The values defined as increase of expression...
... position of AJC motion There was no bony interference with the path ofthe ATFL and CFL The position ofthe AJC during MR scanning was defined as the neutral reference position The length ofthe ATFL ... study the average length ofthe ATFL was 15.8 ± 2.9 mm and the average length ofthe CFL was 27.7 ± 2.7 mm in the neutral position The study of Burks and Morgan measured the length ofthe longest ... distances between the centroids ofthe digitized attachment areas on talus, calcaneus and fibula Once the position ofthe AJC was reproduced in the modeling software, the length ofthe ATFL and CFL...
... and therefore on the final strength ofthe bone-implant structure Therefore the per-operative characterization ofthe primary stem-femur contact and the assessment of primary stem stability in the ... more intricate deformation modes ofthe femur in combination with deformation modes ofthe prosthesis In the case of simple bending modes ofthe femur the prosthesis stem acts just like an added ... ofthe surgical team as presented in Figure (right) and Figure The FRF changes were used as indicators ofthe evolution ofthe stiffness ofthe implant-bone structure and, as a consequence, the...