the temporal dimension of zebrafish intestinal epithelium renewal

... expression of these genes in the intestinal epithelium of zebrafish at a level of single cell The main aim of this thesis is to produce a detailed study and analysis of the zebrafish intestinal epithelium ... epithelium renewal Chapter The spatial orientation of zebrafish intestinal epithelium renewal The spatial orientation of zebrafish intestinal epithelium renewal by positive marking of epithelial ... Chapter The spatial orientation of zebrafish intestinal epithelium renewal 30 The spatial orientation of zebrafish intestinal epithelium renewal – by positive marking of epithelial...

Ngày tải lên: 10/09/2015, 09:25

... μl of PBS in the left ear DTH response was calculated as the percentage swelling (the difference between the increase of thickness of the right ear and the left ear, divided by the thickness of ... the co-cultures The addition of these inhibitors resulted in the abrogation of the inhibition of Tcell proliferation by wild-type MSCs (Figure 3e) The addition of the IDO inhibitor 1-methyl-DL-tryptophan ... μg of CII in the right ear and vehicle in the left ear Delayed-type hypersensitivity responses were measured as the percentage of swelling (100 × [(thickness of the right ear – thickness of the...

Ngày tải lên: 12/08/2014, 11:23

... ATL, HCs – ATL) The zero value of tax gene transcripts was observed in 60.7% of HAM/TSP patients (34 out of 56), 71.1% of HCs (27 out of 38) and 30.0% of ATL patients (3 out of 10) The medians are ... at least the biclonal integration of HTLV-1 proviral DNA The incidence of multibands in our cases (two out of ten: 20%) was comparable with the data by Kamihira et al (17.8%) The Page of 11 (page ... load = value of tax/value of HPRT HTLV-1 HBZ mRNA load = value of HBZ/value of HPRT We used aliquots of the same standard MT-2 cDNA preparation for all assays and the correlation values of standard...

Ngày tải lên: 12/08/2014, 23:20

... deleted from the C terminus of GagPol Figure 1B shows Western blots of lysates of the viruses produced from the different transfections, probed with anti-CA, and shows that all forms of GagPol deletion ... sequences including the thumb domain of RT are deleted (∆581 and ∆715) To measure the amount of tRNALys3 annealed in vivo to the viral RNA genome, total viral RNA was used as the source of primer/template ... Figure 2B The left side of panel A shows that there is a linear increase in the reverse transcription signal over an almost 10 fold change in the amount of BH10.P- viral genomic RNA used in the reac-...

Ngày tải lên: 13/08/2014, 13:20

... monitoring the therapeutic effect of HSV-TK, U87MG cell clones stably expressing the HSV-TK-Luc fusion protein were generated by selection of transfected cells with geneticin Comparison of 18 of these ... mouse prior to sacrifice The tumor was cut in the middle and placed with the cut side facing the CCD camera One part of the tumor was obviously hemorrhagic while the other part looked vital Photon ... conversion of prodrug by HSV-TK was however not measured in either of the above studies Ponomarev et al [24] did not present data on the correlated expression of the three reporters within the triple...

Ngày tải lên: 14/08/2014, 19:22

... major contributor for the clearance of NPs, thus the reducing rate of MPS uptake could be considered as the best strategy for prolonging the circulation of NPs (Li & Huang, 2008) Therefore, PA/EPI ... respectively The bioavailability of free EPI was 100%, the BAR of PA/EPI calculated through the equation above by the AUC0–24h was 189%, much higher than the free drug According to the previous ... toxicity of the PANs was found in the experimental animals after i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI...

Ngày tải lên: 23/04/2013, 21:38

... comparison, the amount of GroEL was determined in the same extracts using anti-GroEL Ig It has been previously shown that at the beginning of the stationary phase the rate of synthesis of heat-shock ... respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing determined amounts of the purified UP12 were separated ... Taken together, these observations indicate that maintenance of the correct folding state of GatY in E coli probably requires the assistance of two chaperone systems The identification of UP12 as...

Ngày tải lên: 22/02/2014, 07:20

... PP The concentrations of porphyrins used in the growth medium are given in Table In the case of Ru-meso, a low concentration had to be used because of the toxicity of this compound Production of ... suggested that the metal ion of the porphyrin is removed during transport of the porphyrin from the medium to the catalase protein in the cytoplasm Alternatively, but less likely, the metalloporphyrin ... 700 nm (C) Spectra of catalases produced in the presence of Cu-PP, Mg-PP, and PP The intensities of these spectra have been normalized with respect to the Soret band absorption The inset in (C)...

Ngày tải lên: 06/03/2014, 11:20

... constrain the operation of the network However, the response for a specific system is constrained by the connection between these elements The response is also influenced by the relative dominance of these ... as the binding constants and the extent of autoregulation The regulatory proteins may reside either in the nucleus or in the cytoplasm or in both, thus controlling the network The existence of ... example, the operation of the switch is constrained by the number of binding sites and the amounts of regulatory proteins Further, numerous elements such as stoichiometry (dimerization), number of...

Ngày tải lên: 07/03/2014, 16:20

... modifications of chromatin [9] Little is known, however, of the functional role of the remaining subclass members In this study, the yeast protein SPP1 (alias cps40), a member of the COMPASS complex ... constitute CGBP homologues The presence of a TFS2M domain is also indicative of a possible function for the DIDO1 long isoform (Figs and S2) It is the second domain of the elongation factor, TFSII ... in the tree (Fig 1), the branch of the FEBS Journal 272 (2005) 3505–3511 ª 2005 FEBS A M Rojas et al fungal sequences is clearly distant from the other branches, which was indeed predicted The...

Ngày tải lên: 07/03/2014, 21:20

... from the administered [75Se]Der p2, indicate together with its own remaining intact levels (the 16 kDa band) that the clearance and further metabolism of the allergen was altered as a result of the ... conclude that the inflammatory state of the lung influence on the rate of metabolism and clearance of Der p Thus, an allergic response may lead to prolonged retention of the allergen in the airways ... detected in the lungs of sensitized mice than in nonsensitized animals The distribution of radioactivity in the other investigated organs did not differ due to the sensitization Thus, the allergen-induced...

Ngày tải lên: 07/03/2014, 21:20

... of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G in the transformed knock-out line does not reach the level of the ... topology where the loop is in the thylakoid lumen, and the N-and C-termini face the stroma, opposite to that of PSI-K On the other hand, the second scenario implies a stromal loop, like that of PSI-K, ... integration of the subunit into the PSI complex A protein band smaller that the tagged versions of PSI-G is present in the lanes with the loop-tagged PSI, but is also seen faintly in the lanes with the...

Ngày tải lên: 07/03/2014, 21:20

... A-deficient rats In the liver of rats with hypervitaminosis the results showed a decrease in the amount of p53 and an increase of the c-Jun The figure shows that the expression of p53 is time course ... analyzed by the Levene test; in those cases in which the variances were unequal, the data were adequately transformed before the ANOVA The null hypothesis was accepted for all the values of these sets ... explain the importance of keeping vitamin A of the incidence of cancer, cardiovascular disease, or death status within the normal range, because p53 tumor 21 from all causes [37] The failure of these...

Ngày tải lên: 08/03/2014, 08:20

... effects of EBI compounds on the plasma membrane H+-ATPase of U maydis Furthermore, the utilization of two different targets in the same biosynthetic route permitted us not only the confirmation of the ... in the indirect effects of sterol modification, namely, the activation of plasma membrane H+-ATPase through changes in the fatty acid profile There have been numerous reports on the influence of ... sterols plus, in the case of EBI-treated sporidia, other collateral effects of these compounds [33] In U maydis, changes in the plasma membrane 18 : 2/18 : ratio followed the inhibition of growth rate,...

Ngày tải lên: 08/03/2014, 22:20

... the fluorescence dye from the quenching molecule by the Dicer enzyme, which cleaved the synthetic let-7 precursor miRNA (Fig 1D) [19] In the absence of the Dicer protein, the emission energy of ... present there is cleavage of the end of the pre-miRNA, and activated fluorescence signals are observed, which implies that the fluorescence beacon system is useful for the detection of the functional ... abnormalities occurring during each of the steps of miRNA biogenesis Second, the oncogenic characteristics of the miRNAs regulate the progression of cancer by preventing the tumor suppressor genes from...

Ngày tải lên: 16/03/2014, 01:20

... aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially expressed isoform, together ... occurred in the brain of NMS rats, although a small fraction of the native 160 kDa synthase was converted into the still active 130 kDa form (Fig 4A) The level of HSP90 remained unchanged during the ... following the addition of IgG1-HSP90 antibody to crude extracts of brain or aorta, NOS was immunoprecipitated, indicating a specific association of the two proteins We then determined the amount of each...

Ngày tải lên: 30/03/2014, 04:20

... daily The concentration is reported as the final molar concentration in the organ bath Endothelium-independent vasorelaxation was tested after mechanical endothelium removal of the endothelial ... http://www.translational-medicine.com/content/7/1/41 Figure Diagram 3of the kinetics of wound closure (A) Diagram of the kinetics of wound closure (A) VEGF165 and QK accelerate the closure of full thickness punch biopsy wounds ... published by the National Institutes of Health in the United States (NIH Publication No 85-23, revised 1996) and approved by the Ethics Committee for the Use of Animals in Research of "Federico...

Ngày tải lên: 18/06/2014, 15:20

... to the logarithm of the initial amount of the target DNA in the sample The relative concentration of one target with respect to another is reflected in the difference of the cycle number, the ... point of view very attractive, the analysis of the correlation of the expression levels of the two forms L and I in the tumor samples not support it at present Decrease in the level of the L ... sample) The SD of ΔΔCt is the same as the SD of the ΔCt The ΔCt values of both the tumor and normal samples are standardized to HGPRT used as endogenous control The values defined as increase of expression...

Ngày tải lên: 18/06/2014, 16:20

... position of AJC motion There was no bony interference with the path of the ATFL and CFL The position of the AJC during MR scanning was defined as the neutral reference position The length of the ATFL ... study the average length of the ATFL was 15.8 ± 2.9 mm and the average length of the CFL was 27.7 ± 2.7 mm in the neutral position The study of Burks and Morgan measured the length of the longest ... distances between the centroids of the digitized attachment areas on talus, calcaneus and fibula Once the position of the AJC was reproduced in the modeling software, the length of the ATFL and CFL...

Ngày tải lên: 20/06/2014, 01:20

... and therefore on the final strength of the bone-implant structure Therefore the per-operative characterization of the primary stem-femur contact and the assessment of primary stem stability in the ... more intricate deformation modes of the femur in combination with deformation modes of the prosthesis In the case of simple bending modes of the femur the prosthesis stem acts just like an added ... of the surgical team as presented in Figure (right) and Figure The FRF changes were used as indicators of the evolution of the stiffness of the implant-bone structure and, as a consequence, the...

Ngày tải lên: 20/06/2014, 01:20