... Mitsuhashi A, Yamazawa K, Nagai Y, Tanaka N, Mat-sui H & Sekiya S (2004) Correlation between MUC5ACexpression and the prognosis of patients with adenocar-cinoma ofthe uterine cervix. Ann ... by the mAbsagainst M1.Immunoreactivity of mAbs against M1 towards a recombinant MUC5AC C-terminal cysteine-richpartTo test the reactivity ofthe mAbs against M1towards the human MUC5AC C-terminal ... part of the figure. The GDPH-cleavage site as well as the epitopes for the mAbs against myc (amyc) and His5(aHis5) are indicated. M-C1,N-terminal cleavage fragment; C2-H, C-terminal cleavage...
... quota may take place via a variety of administrative and market-based mechanisms including private sales and quota exchanges. MS are able to determine whether transfers take place at national, ... 12,312,020,617,019,923,910,46,711,710,114,824,825,08,814,116,817,315,710,88,915,915,128,725,217,613,733,133,50,05,010,015,020,025,030,035,040,0BelgiumBulgariaCzek Rep.DenmarkGermayEstoniaIrelandGreeceSpainFranceItalyCyprusLatviaLithuaniaLuxembourgHungaryMaltaNetherlandsAustriaPolandPortugalRomaniaSloveniaSlovakiaFinlandSwedenUnited ... quotas can be transferred to another producer through either the transfer of an entire farm, the leasing or purchase of quota, or the allocation of quota from a national reserve. The transfer of...
... H-bonddonor leads to rapid O2off-rates [19]. Some ofthe mostunusual Hbs characterized to date are those from nematodesand trematodes (mammalian parasites) such as the nematodeAscaris suum (As) [11,12] ... away from the heme normal (z¢ axis), a is the angle between the projection ofthe tilt ofthe z axis on the x¢,y¢ plane (defined direction of tilt of z and the x¢ axis), and j % a + c defines the location ... [55] had proposed a distal Tyr at position E7 as the source ofthe H-bond to ligand on the basis of a partial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree...
... between that of the monomeric and the dimeric form ( see above) inmutant PufX54*. As in these last two mutants a short lagwas observed (see Table 2), apparently the presence of a stable dimer ... via the plasmid pRKX (in trans) into the host Rb. s phaeroides DQx/g, deprived ofthe chromosomal copy ofthe puf operon.Table 1. Bacterial strains and plasmids. The plasmid host strain in all the ... time ofthe flash was varied stepwise:for each lag period the amplitude and r ate constant of the exponential function were optimized using a nonlinear v2minimization routine [26] an d a plot of...
... at d 2.01–2.10.These data together suggest that the tetrasacchariderepeating unit ofthe polysaccharide consists of two residues of GlcNAc and one residue each of GalNAc, GlcA andAlaLys. The 1Hand13C ... gel-per-meation chromatography on Sephadex G-50. Analysis of the polysaccharide using an amino-acid analyzer revealed the presence of GlcN and GalN in the ratio 2 : 1 as well asanother amino component. ... O-polysaccharide of Pr. alcalifaciens O23 [23], and an amide ofthe same aminoacid withD-galacturonic acid (structure 2) in the O-polysac-charides of P. mirabilis O13 [24]. An amide of D-galacturonicacid...
... substitution of Arg46on intracellular signalling The stimulation of accumulated InsPs by increasingconcentrations of AVP, was assayed for each ofthe 19mutant constructs and the dose–response characteristicscompared ... substituted at position 46 and the biologicalcharacteristics ofthe mutant receptors assessed. None of the other amino acids could replace Arg46 whilst still main-taining the wildtype pharmacological ... UK).Mutant receptor constructsMutation of Arg46 to each ofthe 19 encoded amino acidswas made by a PCR approach. Mutant sense oligonucle-otides (5¢-GGGGGCCTTAGGGGACGTAXXXAATGAGGAGCTGG-3¢) contained...
... had an estimated Tm of 82 °C. The reverse flanking primer sequence, 5¢-ATATACATATGAAAGAAAG-3¢, had a calculated Tm of 42 °C. The mutagenic primers for each variant are: P1 9A (5¢-AGAGCTGCTAGCAGAGTTG-3¢) ... Each of these variants retains a catalytic activity against yeast RNA comparable with that of parent mBS, indicating that a native conformation ispresent. A further indication ofthe similarity ... introduction of Ala for Pro19 makes mBS more similarto RNase A, which has a thermal stability higher than that of mBS. However, P1 9A/ L28Q-mBS has the same thermalstability ofthe parent protein,...
... 11507–11512.Supplementary material The following supplementary material is availableonline:Fig. S1. The SARS N protein is phosphorylated bySRPK1 but not by Clk1 and PKA.This material is available as part ofthe ... environmental stress,mRNA metabolism is reprogrammed to adapt tostress-induced damage. Translationally stalled mRNAstogether with a number of translation initiation factorsand RNA-binding proteins are ... Institute of Molecular Medicine, National Tsing Hua University, Hsin-Chu, TaiwanAn outbreak of severe acute respiratory syndrome(SARS) occurred primarily in Asia in 2003. The causa-tive agent of SARS...
... 3.Deamination ofthe de-O,N-acylated LPS and preparation of oligosaccharides 4 and 5 The mixture of oligosaccharides obtained after alkalinedeacylation ofthe LPS (200 mg) was treated with 300 mgNaNO2in ... from other Pectinatus strains show a lowmolecular mass band ofthe same mobility as in the strain E-82164, and a ladder-like pattern, characteristic of the presence ofthe O-chain. No bands analogous ... 1.Deamination ofthe products of complete deacylation of the LPS led to the oligosaccharides 4 and 5, representingundecasaccharide and pentasaccharide fragments of oligo-saccharides 1a and/or 2. These...
... Results are the mean and standard deviation of triplicateassays. For the determination of apparent kcatvalues, experimentswere performed at a saturating concentration of NADPH (200 lM),over a ... potentials.Steady-state enzyme activities The catalytic activity ofthe NR1-FAD/NADPH domainwas examined and compared with the CPR FAD/NADPHdomain. The CPR FAD/NADPH domain retains trans-hydrogenase ... 2A) . In the case ofthe NR1-FAD/NADPH domain, a second 2¢,5¢-ADP-Sepharose affinity step was also incor-porated in the purification scheme, taking advantage of itsnucleotide binding capacity of...
... countries about the content of these annual reports.TABLE 3RESULTS OFTHE COURT’S ANALYSIS OFTHE CONTENT OFTHE LAST ANNUAL REPORT AVAILABLE AT THE TIME OFTHE AUDITSubject Argentina Israel IndiaNew ... countries‘2. Each Member State shall inform the other Member States and the Commission of each authorisation granted pursuant to this Article, including information on the production standards and control ... organic products3861. The Commission’s analysis of annual reports is not standardised (e.g. no checklists or standard report formats are used) and the analysis does not lead to specific actions...
... e1–219variants (e1–220 with additional Pro), the forward pri-mer 5¢-GGTGTAGAATTCAAGAACGAGGAACTGCG-3¢was combined with (a) 5¢-ATAGTTTAGCGGCCGCTTACTTCCGGCGGATGATGAGCGAG-3¢ for e1–219,(b) 5¢-ATAGTTTAGCGGCCGCTTAGTGATGGTGATGGTGATGCTTCCGGCGGATGATGAGCGAG-3¢ ... solution ofthe structure of the mammalian AChR molecule. A prerequisite forthis is the availability of large amounts of native, sol-uble AChR molecules, a target that can be partiallyachieved ... 5¢-ATAGTTTAGCGGCCGCTCAATGGTGATGGTGATGGTGCTTGCGGCGGATGATGAG-3¢. For the c1–218 variants (some with an additional C-terminal Progiving c1–219), the forward primer 5¢-GGTGTAGAATTCCGGAACCAGGAGGAG...